Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

수지상 내생균근 Glomus Etunicatum의 접종이 석탄폐광지 토양에 식재한 아까시나무의 생육 및 생리에 미치는 영향

홍승진,임경민,박용우,구창덕,우성민 忠北大學校 農業科學硏究所 2012 農業科學硏究 Vol.28 No.2

본문참고

Genetic algorithm을 이용한 supply chain network에서의 최적생산 분배에 관한 연구

임석진,정석재,김경섭,박면웅 한국경영과학회 2003 한국경영과학회 학술대회논문집 Vol.- No.1(1)

Recently, a multi facility, multi product and multi period industrial problem has been widely investigated in Supply Chain Management (SCM). One of the key issues in the current SCM research area involves reducing both production and distribution costs. The purpose of this study is to determine the optimum quantity of production and transportation with minimum cost in the supply chain network. We have presented a mathematical model that deals with real world factors and constraints. Considering the complexity of solving such model, we have applied the genetic algorithm approach for solving this model computational experiments using a commercial genetic algorithm based optimizer. The results show that the real size problems we encountered can be solved in reasonable time.

치아 우식부의 기계화학적 제거 효과에 대한 연구

임순빈,최경규,박상진 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.3

Mechanical removals in decayed teeth have been performed using drill and sharp hand instruments. These methods have some disadvantages such as pain, local anesthesia and overextended cavities Therefore chemo-mechanical excavation of dentin carious lesions has been introduced. The purpose of this study was to evaluate the efficacy of traditional mechanical methods using burs and chemo-mechanical methods (Carisolv) of caries dentin. Mechanical caries removal was carried with low speed round bur Chemo-mechanical caries excavation was performed with Carisolv (Medi-team), using the Carisolv hand instruments. The mean time to remove caries with two different methods was evaluated and the data analyzed with SPSS software (ver 11.5) by t-test (p < 0.05). For histomorphometry of caries removal were also carried with mechanical or chemo-mechanical (Carisolv) methods from 20 extracted caries permanent molars. Complete caries removal was verified with a #23 sharp explorers, Caries Detector (Kuraray Co. Japan), and standard apical radiography. 1. Chemo-mechanical method was taken more times than mechanical method (1.5 fold) (p < 0.05) 2. Excavation for caries took more time for molar lesion than premolar lesion, and the least time was taken to remove the caries in incisor lesion (p < 0.05). 3. There were no significant differences to remove the caries between the maxilla and mandible (p > 0.05). 4. The remaining carious dentin was detected after the ckemo-mechanical removal of the carious dentin, and no smear layer were seen after the mechanical and chemo-mechanical removal of the carious dentin. 본 연구는 우식의 기계적 제거와 기계화학적 제거의 효과를 비교, 평가하고자 하였으며, 기계적 제거방법은 저속의 round bur로, 기계 화학적 제거 방법은 Carisolv로 우식을 제거한 후, 잔존 우식을 확인하여 소요시간을 측정하였고, 또 실험실에서 발치한 우식 대구치 20개를 위 두 가지 방법으로 우식을 제거 후, Villanueva bone stain액으로 염색하고 표본을 제작 후 관찰하여 다음의 결론을 얻었다; 1.우식제거에 평균소요시간은 기계화학적 제거가 기계적인 제거보다 1.5배 더 길게 나타났다 (P < 0.05). 2, 우식제거에 평균소요시간은 소구치보다 대구치에서 더 길었고, 전치에서 가장 적은것으로 나타났다 (P < 0.05) 3. 상 · 하악에서는 차이가 없었다 (P > 0.05) 4. 기계화학적 방법으로 우식 상아질을 제거 시 잔존 우식 상아질이 관찰되었으며 두 방법 모두 우식 상아질을 제거한 표면에서는 도말층을 관찰할 수 없었다.

표준 셀 라이브러리 P&R 포팅과 테스트 칩의 설계

임호민,김남섭,김진상,조원경 한국항행학회 2003 韓國航行學會論文誌 Vol.7 No.2

본 논문에서는 최신의 미세공정인 0.18㎛ CMOS 공정을 이용한 표준 셀 라이브러리를 설계하고, 이를 P&R(Placement and Routing) CAD 틀에 사용할 수 있도록 포팅한다 제작결과를 검증하기 위하여 간단한 테스트칩을 제작하였으며 설계에 사용된 표준 셀 라이브러리는 0.18㎛ 아남반도체의 공정이다. 이러한 설계 및 제작과정을 통하여 최신의 미세공정을 이용하여 디지털 시스템의 자동설계가 가능함을 확인하였다. In this paper, we design standard cell libraries using the 0.18um deep submircom CMOS process, and port them into a P&R (Placement and Routing) CAD tool. A simple test ship has been designed in order to verify the functionalities of the 0.18um standard cell libraries whose technical process was provided by Anam semiconductor. Through these experiments, we have found that the new 0.18um CMOS process can be successfully applied to automatic digital system design.

백서의 실험적 치아이동시 connexin 43의 발현에 관한 연구

임정현,강경화,이종진,김은철,김상철 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.5

치아이동시 골세포간 활성 조화에서 세포간 교통의 중요성이 차츰 인식되고 있으며 세포간 교통을 위한 교통반의 존재가 확인되고 있다. 동물실험 모델에서 실험적 치아이동을 통해 조성된 압박 및 견인측 치주인대에서 교통반 단백의 일종인 connexin 43의 발현을 관찰함으로써 인접 연조직 및 골 조직 내에서 세포 신호 전달 양상의 한 부분을 파악하고자 하였다. Sprague-Dawley계 백서 27마리를 대조군(3마리)과 실험군(24마리)으로 나누었으며, 실험군은 견인력(75g)을 가한 후 12시간, 1일, 4일, 7일, 14일, 28일이 경과한 후 각각 4마리씩 희생시켜, connexin 43의 발현을 면역조직화학적으로 관찰한 바 다음과 같은 결과를 얻었다. 1.대조군에서의 connexin 43 발현은 치은, 상아질, 치주인대, 치수, 골세포에서 경미하였다. 2.교정적 치아이동에 반응하는 치수, 치주인대, 골모세포, 파골세포, 골세포부위에서 connexin 43의 발현이 많았으나 치은, 상아질, 상아모세포에서의 발현은 견인력 적용기간에 관계없이 대조군과 큰 차이가 없었다. 3.치수에서 connexin 43의 발현은 교정력을 가한 4일에서부터 증가하여 7일째까지 크게 증가한 후 14일째부터 감소되었으며 28일째에는 대조군과 차이가 없었다. 4.치주인대에서 connexin 43의 발현은 주로 혈관을 중심으로 치조골 면을 따라 압박측에서 주로 나타났는데 교정력을 가한 12시간에서부터 증가하여 4일에서 가장 많은 발현을 보인 후 7일부터 감소하기 시작하였다. 5.골모세포 및 골세포에서 발현은 실험 1일째부터 출현해 4일째에 가장 많은 발현을 보였고 7일 이후에는 크게 감소하였으며 파골세포에서의 발현은 골모세포와 골세포보다 실험 4일째에 더 많이 관찰할 수 있었다. Bone remodeling in response to force requires coordinated actions of osteoblasts, osteoclasts, osteocytes, and periodontal ligament cells. Coordination among these cells may be mediated, in part, by cell-to-cell communication via gap junctions. This study was designed to evaluate the expression of gap junction, connection 43 in periodontal tissue during the experimental movement of rat's incisors, by LSAB(labelled streptavidine biotin) immunohistochemical staining fort connexin 43. Twenty seven Sprague-Dawley rats were divided into a control group(3 rats), and 6 experimental groups(24 rats) where 75g of force was applied from helical springs across the maxillary incisors. Rats of experimental groups were sacrificed at 12 hours, 1, 4, 7, 14 and 28 days after force application, respectively. And the tissues of a control group and experimental groups were studied immunohistochemically. The results were as follows : 1.In control group, the expression of connexin 43 was rare in gingiva, dentin, cementum, periodontal ligament and bone cells. 2.In experimental group, the expression of connexin 43 was increased in pulp, periodontal ligament, osteoblasts, and osteoclasts, comparing to that in control. And it was rare in gingiva, dentin, and odontoblasts regardless of the duration of force application, which was not different from that of control group. 3.The expression of connexin 43 in pulp of experimental group began to increase in 4-day after force application and got to the highest degree at 7-day. And it decreased after 14-day to be similar to that of control group at 28-day. 4.The expression of connexin 43 in periodontal ligament was noted in small capillaries adjacent to alveolar bone, showing higher intensity of immunolabelling after 4-day. And it was stronger in the pressure side than in tension side of periodontal ligament. After 7-day, decrease in connexin 43 expression was observed. 5.The expression of connexin 43 in alveolar bone began to increase 1-day, reached to the highest degree at 4-day, and decreased at 7-day. And the expression in osteoclasts was more than that in osteoblasts or osteocyte at 7-day.

물의 構造에 미치는 尿素의 影響

林龍進,민경은 慶北大學校 1978 論文集 Vol.25-26 No.-

The solubilities of benzene in water and aqueous urea solution were determined by an ultraviolet method at various temperatures between 0°and 40℃. Solubility minimums, ΔH_sol _min corresponding to a zero heat of solution appeared in 1∼3 M aqueous urea solution as well as in water, and they were shifted to lower temperature side in accordance with the increase of urea concentration. Calculated transfer thermodynamic parameters of benzene from water to urea solution and solubility mininmums were interpreted in terms of iceberg formation in solution and also support the concept that urea acts like a structure breaker in water.

심정지환자에서 epinephrine투여시 혈중 catecholamine의 변화

임경수,황성오,이부수,이강현,이진웅,임종천,김영식,김선만,김 현 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Cardiac arrest is a potent stimulus for the release of endogenous catecholamine and high plasma catecholamine concentration has been reported during cardiopulmonary resuscitation(CPR). Few data were present about the clinical effect of high plasma catecholamine and endogenous catecholamine response to exogenous epinephrine administration during CPR. Purpose : This study was designed to evaluate endogenous catecholamine response to cardiac arrest and exogenously administered epinephrine during CPR. Method : In 21 cardiac arrest victims, blood sample for epinephrine and norepinephrine was drawn before and 1 minute after each epinephrine administration during CPR. 1mg of epinephrine was administered immediately after intravenous access and 3 minute interval during CPR. Plasma catecholamines were quantitated by high-performance liquid chromatography. Result : Baseline plasma epinephrine concentraqtion was higher in patients with cardiac arrest than normal controls (985±1627 vs 194±173 pg/ml, p<0.05). Baseline plasma norepinephrine concentration was also elevated in patients with cardiac arrest than normal controls, although statistically insignificnat(1965±4915 vs 360±250 pg/ml). Plasma epinephrine concentration in cardiac arrest patients was significantly elevated after exogenous administration of epinephrine, but its response was blunted after the third does of epinephrine. Plasma norepinephrine concentration in cardiac arrest patients was also elevated, but its response was observed only after the first epinephrine administration. Plasma epinephrine concentration was persistently higher in patients with return of spontaneous circulation (ROSC) than patients without ROSC. there was no significant difference of plasma norepinephrine concentration whether spontaneous circulation was restored or not. Endogenous epinephrine response to exogenous epinephrine was not observed in patients having prolonged arrest time over 20 minutes. Conclusion : Results of this study suggests that significant amount of catecholamine is released by exogenous epinephrine administration in spite of high plasma catecholamine concentration, and degree of endogenous catecholamine response influences resuscitation outcome.

저작근 공간에서 재발된 치성각화낭

임수연,허경회,이원진,최현배,최순철 대한구강악안면방사선학회 2008 Imaging Science in Dentistry Vol.38 No.2

The odontogenic keratocyst (OKC) is a developmental odontogenic cyst typically occurring in the jaws. Since the first description of OKC was published in 1956, the lesion has been of particular interest because of its specific histopathologic features, high recurrence rate, and aggressive behavior. Recurrences most commonly arise within bone at the site of the original cyst. However, as lining cells may find their way into surrounding tissues either from implantation during surgery or from cortical perforation recurrences may arise at a distance from the original cyst. Here, we report a rare case of recurrent OKC which was first developed in mandible and recurred within the masticatory space.

Sertoli 세포의 생리기능 및 호르몬에 의한 조절

임규,박청,윤경아,윤은진,박종일,박승길,황병두 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.2