Effect of Light with Different Wavelengths on Nostoc flagelliforme Cells in Liquid Culture

( Yu Jie Dai ),( Jing Li ),( Shu Mei Wei ),( Nan Chen ),( Yu Peng Xiao ),( Zhi Lei Tan ),( Shi Ru Jia ),( Nan Nan Yuan ),( Ning Tan ),( Yi Jie Song ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4

The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under 27 μmol·m-2·s-1 of light intensity and 25oC on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.

Taguchi Approach for Anti-heat Stress Prescription Compatibility in Mice Spleen Lymphocytes In Vitro

Xiao-yu Zhu,Gui-lin Cheng,Feng-hua Liu,Jin Yu,Yu-jie Wang,Tong-quan Yu,Jian-qin Xu,Ming Wang 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7

Heat stress (HS) may induce immunosuppression as well as inhibit the proliferation of lymphocytes. This study evaluated the effects on immune function of our prescription on splenic lymphocytes under HS as well as its compatibility. The effects of four herbal extracts from Agastache rugosa, Atractylodes lancea, Cortex Phellodendri, and Gypsum Fibrosum on heat treated splenic lymphocytes were investigated and the compatibility of the prescription was also explored by using the Taguchi method. This study revealed changes in proliferation by traditional Chinese medicines of splenic lymphocytes after HS. Proliferation in the HS group was significantly lower than the control group. Under HS, the effects of higher concentrations of Agastache rugosa (100 and 200 μg/mL), Atractylodes lancea (100 and 200 μg/mL), Cortex Phellodendri (50 and 100 μg/mL) and Gypsum Fibrosum (100 and 200 μg/mL) caused a significant increase on ConA/LPS-induced proliferation of lymphocytes than lower concentrations. We, therefore, conclude that the prescription of traditional Chinese medicines may recover splenic lymphocytes from the immunosuppression induced by HS. The Taguchi design, which allows rapid and high efficiency for the selection of the best conditions for our prescription on HS-treated splenic lymphocytes, demonstrated that Agastache rugosa (200 μg/mL), Atractylodes lancea (200 μg/mL), Cortex Phellodendri (100 μg/mL) and Gypsum Fibrosum (100 μg/mL)were the optimal conditions for the prescription. The validation experiment confirmed that our composition in optimum extraction conditions enhanced effects on ConA or LPS-stimulated lymphocytes under HS. The results showed that the Taguchi optimization approach is a suitable method for optimization of the composition of prescription.

Clinicopathological Features and Prognosis of Gastroenteropancreatic Neuroendocrine Tumors: Analysis from a Single-institution

Zeng, Yu-Jie,Liu, Lu,Wu, Heng,Lai, Wei,Cao, Jie-Zhi,Xu, He-Yang,Wang, Jie,Chu, Zhong-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: The gastroenteropancreatic neuroendocrine neoplasm (GEP-NEN) is the most common type of neuroendocrine neoplasm. We summarized data in our centre to investigate the clinicopathological features, diagnostic methods, therapeutic approaches and prognosis for this neoplasm to increase knowledge of this disease in Asian populations. Method: A total of 122 patients treated at Sun Yet-san Memorial Hospital of Sun Yat-sen University between January 2000 and December 2011 were analyzed retrospectively. Results: Pancreas was the most common site of involvement (65/122, 53.3%); this disease has no special symptoms; positive rates of chromogranin A (CgA) and synaptophysin (Syn) were 81.1% and 87.7%, respectively. The positive rate of Syn had statistical difference among the three grades, but not CgA. Some 68 patients had G1 tumors, 32 G2 tumors and 22 G3 tumors, and Chi-square test showed that higher grading was correlated with worse prognosis (${\chi}^2=32.825$, P=0.0001). A total of 32 patients presented with distant metastasis, and 8 cases emerged during following up. Cox proportional hazards regression modeling showed that the tumor grade (P=0.01), lymphatic metastasis (P=0.025) and distant metastasis (P=0.031) were predictors of unfavorable prognosis. The overall 5-year survival rate was 39.6%, the 5-year survival rate of G1 was 55.7%, and the G2 and G3 were 34.2% and 0%, respectively. Conclusions: The incidence of gastroenteropancreatic neuroendocrine tumors has risen over the last 12 years. All grades of these diseases metastasize readily, and further research regarding the treatment of patients after radical surgery is needed to prolong disease-free survival.

“The strength to bear my wounds”: Mazeppa and the Quest for Vigor in Adversity

( Yu Jie-ae ) 대한영어영문학회 2009 영어영문학연구 Vol.35 No.2

The purpose of this paper is to investigate the multifaceted sides of Mazeppa’s physical and mental sufferings, revealed by the symbolic ramifications of his compelled expedition into the dark, vast woods. It will examine how Byron’s Mazeppa depicts the procedure in which Mazeppa undergoes his physical and mental torments, unveiling his diverse and complex attitudes toward the adverse states of distress and misery. We will scrutinize the dynamic and subtle layers of Mazeppa’s thoughts and actions, which feature his various responses to the ordeals, and his attempts to resist or embrace them through his willpower. The essay will also consider how Mazeppa’s spiritual journey, accompanying his stoical search for vitality and relief, is enriched with an extensive range of unexpected and ironic consequences, which pave the way for the heroes of Beppo, The Island, and Canto III of Don Juan. (Changwon National University)

John Donne’s Devotions upon Emergent Occasions Ⅱ

Yu, Jie-Ae(유지애) 새한영어영문학회 2015 새한영어영문학 Vol.57 No.2

This article examines how John Donne reflects on the diverse aspects of vicissitudes in Devotions upon Emergent Occasions II. A number of textual passages enumerate the author’s contemplations into changeability of the world in general and his personal life in particular. Through the structure of “Meditation,” “Expostulation,” and “Prayer,” he investigates the thematic concerns with capriciousness, the ironic boundaries of human competence, and his spiritual enlivening into the perpetual presence and working of the Almighty, which remains beyond the mutable realm of human affairs and enterprises.

The Regulation of Root Pruning and Soil Water Content to Apple Leaf Water Use Efficiency

Yu-Ling Jie,Hong-Qiang Yang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

“Under this bizarre transience of life”: The Vicissitude and Irony of Human Endeavors in Byron`s Childe Harold`s Pilgrimage IV

( Jie Ae Yu ) 한국현대영어영문학회 2010 현대영어영문학 Vol.54 No.2

The main purpose of this paper is to examine how Byron`s Childe Harold`s Pilgrimage IV depicts the mutability and ironic results of man`s diverse cultural activities and achievements. A number of textual evidences clearly reveal one of the recurrent motifs, which features Childe Harold`s unswerving meditations on the changeable, unexpected consequences of man`s artistic or literary pursuits and productions. The poet gradually unfolds the multiple layers of the protagonist`s subtle and complex observations into the diverse and mutable aspects of human enterprises, deploying efficient structure, imagery, and tonal complexity to elaborate such thematic concerns. The paper also aims to scrutinize how Childe Harold searches for the undeminishing power and influence of the imperishable spirit which persistently underlies art, nature, and the human mind. Indeed, Childe Harold undertakes a spiritual journey in which he struggles to surpass the transitory sides of man`s efforts and attainments embodied in the external cultural products. (Changwon National University)

Selective Adsorption and Reusability for Pb<SUP>2+</SUP> of Chitosan-based Microporous Polymer

Jie Yu,Jidong Zheng,Quanfang Lu,Xing Wang,Xiaomin Zhang,Qizhao Wang,Wu Yang 한국고분자학회 2017 폴리머 Vol.41 No.3

A green and simple glow-discharge electrolysis plasma (GDEP) technique was used to synthesize the chitosan/attapulgite/poly(acrylic acid-co-2-acrylamido-2-methyl-1-propanesulfonic acid) (CS/ATP/P(AA-co-AMPS)) microporous polymer. The results showed that the optimum pH for the adsorption of Pb<SUP>2+</SUP>, Cd<SUP>2+</SUP>, Co<SUP>2+</SUP>, and Cu<SUP>2+</SUP> is 4.8, and time of adsorption equilibrium is 60 min. Adsorption kinetics fits well to the pseudo-second-order model. The maximum adsorption capacities for Pb<SUP>2+</SUP>, Cd<SUP>2+</SUP>, Cu<SUP>2+</SUP> and Co<SUP>2+</SUP> are 500.0, 301.7, 180.0 and 151.7 mg g<SUP>-1</SUP>, respectively. The microporous polymer has higher adsorption selectivity toward Pb<SUP>2+</SUP> with the coexistence of Cd<SUP>2+</SUP>, Co<SUP>2+</SUP>, and Cu<SUP>2+</SUP>. The CS/ATP/P(AAco-AMPS) possesses promising regeneration and reusability using 2.0 g L–1 ethylenediamine tetraacetic acid tetrasodium salt (EDTA-4Na) solution as eluent. CS/ATP/P(AA-co-AMPS) can be used as a very promising absorbent for the separation purification and selective recovery of Pb<SUP>2+</SUP> in aqueous solution containing Cd<SUP>2+</SUP>, Co<SUP>2+</SUP>, and Cu<SUP>2+</SUP> ions.

Matthew Arnold’s Sohrab and Rustum : Unfurling Characterization and Thematic Manifestations

Yu, Jie-Ae 한국중앙영어영문학회 2021 영어영문학연구 Vol.63 No.4

This article examines how Arnold’s narrative poem Sohrab and Rustum manifests unique features of two main characters in reference to concepts of unknown irresistible power and man’s relentless defiance. This poem, published during the early stage of Arnold’s literary career in 1853, resonates Sophoclean concepts of the clash between fate and human pride. While dealing with Arnoldian awareness of the Greek dramatic allusion, this paper also highlights his anticipation of later figures, for instance, Thyrsis and poetic speakers who reflect the spirit of Sohrab and Rustum but show their own unique personalities. It also argues that Arnold’s characterization in Sohrab and Rustum establishes key concepts of the bounds of human wishes and enterprises against their adverse forces. While scrutinizing this aspect, the paper relates this aspect to Arnold’s earlier works. In addition, it demonstrates that his portrayals of the characters foreshadows a wide range of the subsequent construction of his literary oeuvre which associates with the speaker’s elegiac lyric sensitivity to the era of spiritual turmoil and the author’s dynamic portrayals of the paradox and irony of man’s life and the world.

Purification and Identification of Antioxidant Peptides from Enzymatic Hydrolysate of Spirulina platensis

Yu, Jie,Hu, Yuanliang,Xue, Mingxiong,Dun, Yaohao,Li, Shenao,Peng, Nan,Liang, Yunxiang,Zhao, Shumao The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7

The aim of this study was to isolate antioxidant peptides from an enzymatic hydrolysate of Spirulina platensis. A novel antioxidant peptide was obtained by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography, with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay used to measure the antioxidant activity, and the sequence was determined to be Pro-Asn-Asn (343.15 Da) by electrospray ionization tandem mass spectrometry. This peptide was synthesized to confirm its antioxidant properties, and it exhibited 81.44 ± 0.43% DPPH scavenging activity at 100 μg/ml, which was similar to that of glutathione (82.63 ± 0.56%). Furthermore, the superoxide anion and hydroxyl free-radical scavenging activities and the SOD activity of the peptide were 47.84 ± 0.49%, 54.01 ± 0.82%, and 12.55 ± 0.75%, respectively, at 10 mg/ml. These results indicate that S. platensis is a good source of antioxidant peptides, and that its hydrolysate may have important applications in the pharmaceutical and food industries.