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Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604
Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3
A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.
박지숙,이민진,정재원,김미혜,이서연,지윤미,한준호,김유학,공명석,한광현,노희명 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2
It is important to interpret the soil chemical properties in order to effectively manage the farmland. This study was conducted to investigate the soil chemical properties of upland soybean field according to the soil series at 32 upland fields located in the parts of Gangwon-do. Soil sampling sites were selected by using the National Statistics DB and KSIS DB that is a soil environment information system provided by the Rural Development Administration. Soil samples were collected from 0~15 cm of top soil before seeding or transplanting, air-dried, passed through a 2 mm sieve, and analyzed for soil pH, EC, organic matter, available phosphorus (P2O5), exchangeable potassium, exchangeable calcium and exchangeable magnesium. The average chemical properties have exceeded the each nutrient optimum range level of RDA, except the organic matter in experimental soil 2015. And despite application of conventional fertilization, each soil series nutrient contents were change respectively. The results of this study suggest that conventional fertilization should be applied based on the analysis about the amount of accumulated nutrient contents in the soil.
자일리톨 섭취에 따른 Streptococcus mutans의 글루칸 생성관련 유전자 발현 억제효과
김지혜,이영은,안상헌,최연희,남순현,송근배 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.4
본 연구에서는 장기적인 자일리톨의 섭취가 Streptococcus mutans의 대표적인 독성인자 중 하나인 글루칸 생성에 미치는 영향을 알아보기 위하여 글루칸 합성효소인 glucosyltansferase의 mRNA 발현을 실시간 역전사 중합효소 연쇄반응을 통해 평가하여 다음과 같은 결과를 얻었다. 1. 24개월 동안 자일리톨껌을 섭취한 군에서 타액 내 Streptococcus mutans의 colony 수는 통계적으로 유의하게 감소하였다(p<0.05). 2. 비수용성 글루칸 합성에 관여하는 유전자인 gtfB, gtfC의 발현은 자일리톨껌을 섭취한 군에서 시간이 지남에 따라 유의 하게 감소하였다(p<0.05). 특히 gtfB의 발현은 12개월과 24개월째 대조군에 비하여 통계적으로 유의하게 낮았고, gtfC의 발현은 24개월째 대조군에 비하여 통계적으로 유의하게 낮았다(p<0.05). 3. 수용성 글루칸 합성에 관여하는 유전자인 gtfD의 발현 역시 자일리톨껌을 섭취한 군에서 시간이 지남에 따라 유의하게 감소하였다(p<0.05). 또한 gtfD의 발현은 12개월과 24개월째 대조군에 비하여 통계적으로 유의하게 낮았다(p<0.05). 이상의 결과들을 종합해 보았을 때, 자일리톨의 섭취는 구강 내 Streptococcus mutans의 글루칸 합성 관련 유전자들의 발현을 억제시킴으로써 Streptococcus mutans의 수적인 감소를 가져오는 것으로 생각된다. Xylitol has the ability to reduce the adherence of Streptococcus mutans(S. mutans), which can make it easier to remove plaque, decrease acid production and inhibit dental caries. There are few reports on the effects of xylitol on the expression of the virulence related genes in S. mutans. This study examined the inhibitory effect of chewing gum containing xylitol on glucan synthesis related gene expression of S. mutans. Participants were voluntarily recruited for a women's oral health prevention program, classified into two groups(a control and a xylitol group), and then followed for 2 years. Twenty salivary samples were randomly selected from each group. Colony count and real-time reverse transcription polymerase chain reaction were used to analyze the characteristics of S. mutans. The following results were obtained: The S. mutans counts decreased steadily in the xylitol group over the study period(p<0.05). The expression of the virulence related genes (gtfB, gtfC and gtfD) was significantly lower in the xylitol group than in the control groups (p<0.05). In conclusion, these results suggest that chewing xylitol gum for a long period of time may reduce the expression of the genes associated with S. mutans virulence, which can result in a decrease growth of S. mutans colonies as a result.
소규모사업장 보건관리기술지원사업 지원여부에 따른 사업장내 의사소통 비교
정최경희,박혜숙,김정연,이경용,현성민,오지영,김수근,김현주,하은희 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2
목적: 소규모사업장 보건관리기술지원사업 지원여부에 따라 사업장내 위험의사소통채널이 마련되었는지와 이 채널이 기능을 하는지, 그리고 의사소통채널마련과 사업장내 위험의사소통 활성화의 기본적이고 중요한 배경이 되는 사업주의 산업보건에 대한 관심이 차이가 있는지에 대해 살펴보고자 하였다. 방법: 서울, 안산, 대전, 광주, 포항 지역에서 보건관리기술지원사업을 받은 사업장과 받지 않은 사업장을 대상으로 비례층화무작위표본추출법을 사용하여 추출한 후 1991년 12월 6일부터 10일간 우편설문조사를 실시하였다. 설문대상은 각 사업장의 남녀 각 1명, 보건업무담당자, 사업주였으며, 설문내용은 사업장 및 응답자의 일반적 특성 및 사업장내 위험의사소통이었다. 설문에 응답이 온 463개 사업장의 자료를 자료처리하여 총 228개 사업장(단해년도지원 사업장 189개, 연속2년지원 사업장 13개, 비지원사업장 26개)을 대상으로 분석하였다. 결과: 사업장내 의사소통에 대한 단변량분석에서는 보건업무 담당자의 선정여부만이 통계적으로 유의한 차이가 있었다. 소규모사업장 보건관리기술지원사업을 지원받지 않은 사업장과 지원받은 사업장의 사업장내 위험의사소통을 비교하기 위한 로지스틱회귀분석 결과, 모든 항목에서 통계적으로는 유의한 차이를 보여주지 않았다. 그러나 사업주의 관심(OR ; 1.63, 95% CI ; 0.38~7.01)과 보건업무담당자의 선정(OR ; 3.67, 95% CI ; 1.00~13.44) 및 결정권한(OR ; 1.48, 95% CI ; 0.25~8.91), 사업주의 산업보건문제 소통(OR ; 3.07, 95% CI ; 0.81~11.71), 보건업무담당자의 정보요청경험(OR; 4.48, 95% CI ; 0.97~20.57)에서는 지원받지 않은 사업장에 비해 지원받은 사업장에서 교차비가 증가하는 양상을 보여주었다. 반면 노동자의 산업보건문제 소통가능성여부는 오히려 교차비가 0.53(95% CI ; 0.11~2.44)로 감소하는 경향을 보여주었다. 결론: 소규모사업장 보건관리기술지원사업을 의사소통의 측면에서 평가해보면, 사업주나 보건업무담당자에게는 다소 효과가 있었지만 노동자가 지속적으로 참여할 수 있도록 의사소통문화를 바꾸어내는 데에는 한계가 있었다. 개선을 위해서는 노동자 중심의 접근 프로그램의 개발 및 시행이 시급하다 할 수 있다. Objectives :This study was to evaluate the effectiveness of the government-funded subsidized occupational health program for small-scale enterprises(GSOHP_SSE) by occupational health com-munication in workplace. Methods : We sampled the 1,835 enterprises of study by proportional stratified random sampling among industries of supported and not supported by GSOHP_SSE in Seoul, Ansan, Daejeon, Kwang-joo and Pohang. Information on the general characteristics of enterprises and respondants and occupational health communication in the workplace was collected on self-reported questionnaires by post-survey between December 6 and 15 in 2001. We received answers from 463 enterprises and analyzed 228 enterprises through data-cleaning by logistic regression to evaluate effectiveness of GSOHP-SSE about occupational health communication in the workplace. Results : By multiple logistic regression analysis, GSOHP_SSE turned out not to be statistically significant factor in all dependent variables about occupational health communication in the work-place. The concern of owner(odds ratio (below OR) ; 1.63, 95% confidence interval(below 95% CI) ; 0.38-7.01), formation(OR ; 3.67, 95% CI ; 1.00-13.44) and empowerment(OR ; 1.48, 95% CI ; 0.25-8.91) of health manager, request about occupational health problem of health manager(OR ;4.48, 95% CI ; 0.97-20.57) and occupational health communication of owner (OR ; 3.07, 95% CI ; 0.81-11.71) had the trend increasing OR in the industries supported GS-OHP_SSE relative to the industries not supported. But in recognition of possibility on occupational health communication of laborers, OR of GSOHP_SSE was decreased in 0.53(95% CI ; 0.11-2.44). Conclusions: This study found that the GSOHP_SSE was some effective to the side of employer and health manager, but had a limitation about change of communication culture useful to the laborers. We suggested the laborer-centered health training programs or the empowerment-based health training programs for effective occupational health management in the workplace.
이지은,장혜경,오지영,유윤경,김현지,임숙인,연숙희,강진숙,최귀령 한국병원약사회 2003 병원약사회지 Vol.20 No.1
In an aging society, there is an increasing possibility of the duplication of the drugs given to patients because they take many kinds of drugs. Among the prescriptions given to the patients who was treated at multiple ambulatory clinics in St. Mary's hospital for one month of September, 2002, in which drugs that has identical or similar effects are prescribed, we analysed and evaluated them by patients' characteristics, kinds of medication, severity of side effects on a case by case basis. More, we assessed the potential additional costs. As a result of this study, the duplication rate turned out to be 6.69% and it was shown that the proportion of the elderly patients over 60 was high. The gastro-intestinal medications took up a large part and most of the cases showed that the danger caused by double taking of medicine was slight, but there were some examples in which the serious side effects were predicted. Expected additional costs were the average 11.349 won and it ranges from the minimum of 56 won to the maximum of 135,720 won. In conclusion, the management of drug histories of the patients who need the plural treatments is very important and the necessity of the individual and professional guidance of taking medicines for the elderly patients is emerged, too.
Ji-Youn Kim,Mi-Sol Park,박정은,Seung-Sook Yang,Min-Jeong Kim,Sun-Ae Mok,Hye-Ji Kim,Chan-Ho Kim,Ki-Jung Kim,Ja-Won Cho 대한예방치과학회 2019 International Journal of Clinical Preventive Denti Vol.15 No.1
Objective: This study was carried out to investigate the effect of dentifrice containing green tea extract, sodium monofluorophosphate and calcium carbonate on dental plaque and gingivitis by periodic measurements. Methods: Oral examination items were measured by papillary marginal attached (PMA) index, Talbott’s gingival index, patient hygiene performance (PHP) index, and Turesky’s plaque index. Results: PMA index showed statistically significant difference in the control group (p<0.05). Talbott’s gingival index measurements showed statistically significant differences in the control group from 8 weeks after the start of the study (p<0.05). As a result of PHP index measurement, statistically significant differences were observed in the experimental group from the control group after 4 weeks (p<0.01). And Turesky’s plaque index measurement, statistically significant differences were observed in the experimental group from the control group after 4 weeks (p<0.01). Conclusion: These results suggest that dentifrice with green tea extract, sodium monofluorophosphate and calcium carbonate can be effective in reducing gingivitis and dental plaque.