Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

박목월의 시 <나그네>의 예술가곡화 연구

이지연 ( Lee Ji-yeon ),김용범 ( Kim Yong-bum ) 한민족문화학회 2016 한민족문화연구 Vol.56 No.-

본 논문은 박목월의 시 <나그네>의 예술가곡화에 대한 연구이다. 예술가곡이란 18세기 독일어권에서 완성된 음악 양식으로, 시인의 시와 작곡가의 작곡 그리고 성악가에 의한 가창으로 표현되는 예술로 문학과 음악이라는 두 가지의 예술 장르가 합일된 문화융합의 산물이다. 이러한 예술가곡이 우리나라에 도입정착 된 후 1920년에 우리나라 최초의 가곡 홍난파의 <봉선화>가 작곡되지만 한국예술가곡의 효시 작품에 대한 논란이 존재하였고 독일 예술가곡의 개념과 정의 및 조건에 입각한 필요충분조건을 모두 갖춘 한국최초의 예술가곡으로는 정지용 작시, 채동선 작곡의 <고향>(1932)으로 비정하고 있다. 특히 1920년대는 음악이나 시문학이 아직은 확고하게 예술장르로 자리 잡지 못한 시기였음으로 시인과 작곡가가 협업을 통해 창작해야하는 예술가곡의 시기로서는 상호 불완전했었다 할 수 있었고 한국문학은 1930년대에 이르러 시인들이 자신들의 창작 행위에 대한 뚜렷한 자의식을 갖게 된다. 그 중 본고가 주목하는 것은 한국 시문학사의 독보적 위상을 차지하고 있는 시인 박목월과 그의 시를 가사로 한 예술가곡들이다. 박목읠의 초기시가 가지고 있는 서정성과 율성 즉 음악성은 작곡가들에게 주목받아 김연준을 비롯하여, 김성태, 김순애, 이수인, 윤이상 등에 의해 많은 예술가곡이 탄생하였는데 예술가곡 작품들을 일별하는 과정에서 유독 <나그네>를 대상으로 작곡된 작품이 많았음을 발견하고 음악적, 문화융합론적, 언어기호학적 관점에서 그 이유를 분석 및 규명해보았다. 시의 정형리듬을 형성하려는 계산에서 이루어진(artificial const-ruction)박목월의 <나그네>는 예술가곡의 작곡에서 조성, 박자, 악절과 결합하기에 최적의 조건이었고 두드러진 유음의 사용은 성악의 발성을 용이하게 하는 요소로 작용된다. 따라서 시인의 시와 작곡가의 작곡뿐 아니라 예술가곡의 필요충분조건의 하나인 노래 불려 져야 한다는 가창의 조건으로 볼 때 나그네가 가지는 예술가곡의 적합성은 예술가곡성립의 필요충분조건과 자질을 모두 갖추고 있음을 알 수 있다. 한 시인의 특정한 작품에 14명의 작곡가가 곡을 붙였다는 것은 우리 예술가곡사 상 특이한 사례에 속하며 이는 박목월 <나그네>의 문학적 가치를 넘어선 문화융합의 새로운 가치를 의미하는 것이다. This study aims to examine the capability of Mok-wol Park`s poetry, "Naguenae", as an art song. The art song, originally developed in Germany in the eighteenth-century, is a genre of art that unites poetry and music; the composers put a melody on poems so that singers could sing the songs. As an outcome of cultural convergence, the two independent forms of art, literature and music, came to converge into a completely single genre, heralding a birth of a new musical style. Korean poetry began to mature in the 1930s and Mok-wol Park, the most representative poet throughout the history of Korean literature, shows lyrical and rhythmical features on his early poems. This musicality was valuable to be used as text for art songs by many contemporary composers such as Yeon-jun Kim, Sung-tae Kim, Sun-ae Kim, Soo-in Lee, and I-sang Yoon. In the process of categorizing their art songs, we discovered that there are numerous songs using particularly "Naguenae" as a text. This study mainly explores and analyzes reasons from musical and glossematic perspectives, as well as from viewpoint of the theory of cultural convergence. Mok-wol`s "Naguenae" written based on a calculation of its regular rhythmic patterns has the optimum condition as a text for the musical setting in which a tonal center, rhythm, and phrasing can be combined without any disturbances. Moreover, his use of liquid sound aids a singer`s vocalization. Therefore, in view of the fact that a song is to be sung, which is a necessary and sufficient condition for an art song, together with a text and musical setting, "Naguenae" qualifies all conditions. The fact that one particular poem written by one poet was used as a text for an art song by fourteen different composers is unusual case in Korean history of art songs. In addition, Mok-wal Park`s "Naguenae" is not only literary valuable, but means a new step of cultural convergence.

가평 전주이씨묘 출토복식 고찰

최연우,박윤미,김윤경,김지희,박양희,이선우,차서연,편나영,황혜남,Choi, Yeon Woo,Park, Yoon Mee,Kim, Yoon Gyung,Kim, Ji Hee,Park, Yang Hee,Lee, Seon U,Cha, Seo Yeon,Pyeon, Na Young,Hwang, Hye Nam 한국의류학회 2017 한국의류학회지 Vol.41 No.5

In 1995, the costumes were restored in an unknown woman's tomb at the Jeonju Lee's family cemetery in Gapyeong, Gyeonggi-Do, and there are currently nine items remaining. In this study, we first introduced these 9 relics to academia and analyzed the morphological characteristics of the costumes. We also estimated the time and person of burial compared with costumes unearthed from other burials. Jeogories (short jacket) are all four items. One of these items was unusual in shape, and the upper part of the seop (gusset) was wrinkled and shaped. This type of Jeogori appears only till the 1520s in other tombs. There are also two skirts, one of which is characterized by a superimposed pattern. The top and bottom / middle part of the skirt were rolled up, and the skirt, which was double-rolled up in this way, was first discovered. An analysis of the shape of Jeogori and skirt indicated that the person buried in the Jeonju Lee's family tomb was believed to have survived until the early 16th century. Based on these estimated periods, as a result of looking at the genealogy of Jeonju Lee's family, it was concluded that the tomb was probably a woman named Lee Geum Myeong (李金命) born in the mid-15C and died between the late 15C and the early 16C.

태아의 하악에서 발견된 이상결절 1례의 연구

이치연,이동주,조이수,김승인,이지연,김은철 원광대학교 생체재료·매식연구소 1996 원광생체재료·매식 Vol.5 No.2

Dens evaginatus is a rare developmental anomaly in which an enamel-covered tubercle projects from the occlusal surface of a tooth. usually lower second premolar. When the tubercle fractures, the pulpal extension into the tubercle may be exposed, which results in early pulpal necrosis, periapical infection and even osteomyelitis. We found the dens evaginatus in 16-week-old fetal mandible. Microscopically, we observed the protrusion of dental papilla into the dental organ and hard tissue formation which will be the tubercle by serial section. This anomaly was reported to be very rare in tooth development of fetus, so we reported this finding with brief review of literatures.

한국예술가곡 효시에 관한 연구

이지연 ( Ji-yeon Lee ),공정배 ( Jeong-bae Kong ),김용범 ( Yong-beom Kim ) 한국사상문화학회 2016 韓國思想과 文化 Vol.85 No.-

본 연구는 독일어권에서 비롯된 예술가곡 리트(Lied)의 한국적 전이과정을 통해 한국예술가곡의 효시를 규명하고자하는 목적이다. 예술가곡은 시문학과 음악의 합일된 예술장르로서 독일과 오스트리아를 포함한 독일어권에서 발달된 가곡으로, 시인의 시에 작곡가가 선율을 붙이고 이를 성악가가 부르는 음악 양식이다. 그 효시를 슈베르트의 `실 잣는 그레첸(Gretchen am Spinnrade)`로 비정하고 있다. 작곡가의 임무란 좋은 시를 널리 알리는 것이었기 때문에 음악은 시에 종속되어 있었다. 그러나 예술가곡은 상호매체성에 의한 문화융합결과물로서 시와 음악이 서로가 각기 독립된 예술이었던 상태로부터 벗어나 완전히 하나로 융합되면서 또 하나의 새로운 양식으로 탄생한다. 슈베르트 이후 가곡의 작곡자는 더 이상 시의 언어 음율을 선율적으로 고양시키는 낭음자가 아니라 가사에서 말로 표현될 수 없는 영역을 음악적으로 표출하는 시의 자유로운 해석자의 역할을 하게 된다. 음악을 단순히 시를 쉽게 이해하는 보조수단에 국한시킨 이전의 가곡미학을 극복하고자 하는 새로운 경향들이 나타나면서 이전까지 작법인 유절가곡이 아닌 통절 작곡 형식이 새롭게 떠오르기 시작하였다. 이렇게 완성된 예술가곡은 1835년경 이후부터 독일을 벗어나 다른 나라로도 전파되어, 각 나라의 음악적 특징에 맞는 독자성을 지니며 민족음악으로 자리잡는다. 그리하여 그 갈래의 하나가 한국에 이르게 된다. 독일 예술가곡의 개념과 정의 및 조건에 맞는 한국 최초의 예술가곡이 어떤 작품인가는 학자들의 견해에 따라 논란이 존재 한다. 그러나 이 관점은 예술가곡을 시와 음악의 등가의 결합을 전제로 보았을 때 우리 시문학사적 위상과 일치하는 시기이다. 한국 시문학사는 1930년대에 이르러서 성숙함이 묻어나면서 시인들은 창작 행위에 대한 확고한 자신의 색깔을 드러내기 시작했다. 이 시기에 전문적인 시인의 등장이 활발해지는데 이때 정지용을 대표적으로 꼽을 수 있다. 1932년 7월 동방평론 4호에 발표한 정지용의 시 `고향`에 작곡가 채동선이 곡을 붙였고, 채선엽이 독창을 맡았으며 반주는 김애식이 맡았다. 작곡가 채동선은 독일에서 정통으로 작곡을 공부하고 귀국하여 <고향>을 작곡하였고 시기적으로는 물론 예술가곡의 완성의 필요충분조건은 모두 갖춘 한국 최초의 예술가곡 그 효시로 비정할 수 있다. This study aims to clarify the beginning of the Korean art song by reviewing how the German art song "lied" was traditionally transferred and spread in Korea. As a genre of art that unites poetry and music, the art song was originally developed in German-speaking countries including Germany and Austria; the then composers put a melody on poems so that singers(vocalists) could sing the songs. Gretchen am Spinnrade by Franz Peter Schubert is considered the beginning of such a style of music. Since promulgating good poems was one of the composer`s missions then, music was viewed as subordinate to poems. As an outcome of cultural convergence through intermediality, however, the two independent forms of art, poetry and music, came to converge into a completely single genre, heralding a birth of a new music style. Since Shubert, composers were no longer a mere reciter exalting poems by putting melodies on rhythmical poetic language; instead, they served as a free interpreter of poems to express the sentiment beyond the lyrics musically. In an attempt to overcome the stereotype that regarded music simply as an aid to better understand poems, a new trend such as through-composed songs, away from traditional strophic songs, emerged. Those German art songs were widely disseminated beyond Germany since 1835, and took root in many areas as folk music combined with each country`s traditional musical hallmarks. Korea was no exception. With Korean art songs originated from Germany, scholars in the field have long disputed over which one is the first Korean art song that complied with the concept, definition, and conditions of the German lied. When we consider the art song as a fair combination of poetry and music, a hint can be found from the history of Korean poetry. Korean poetry began to mature in the 1930s with poets clearly expressing their own signature colors in their creations. Professional poets actively broke onto the art scene, and Ji-yong Jeong was one of them. Mr. Jeong released a poem titled "Home (Gohyang)" on No. 4 Dongbang Critique in July, 1932, and Dong-sun Chae, a composer, put a melody on it; later it was sung by Sun-yub Chae with the piano accompaniment by Ae-sik Kim. After studying composition officially in Germany, Dong-sun Chae returned to Korea and composed the song, meeting all of necessary and sufficient conditions of the German lied. In this sense, "Home (Gohyang)" can be regarded as the first Korean art song.

한라산 주변에 발생하는 Karman Vortex Street 재현

권지혁(Kwon Ji-hyuk·Lee),이연원(Lee Yeon-won),김영덕(Kim Young-duk),박광열(Park Kwang-yul) 한국풍공학회 2006 한국풍공학회지 Vol.10 No.1

늦가을과 초본 사이 제주도 후면에 Karman vortex street가 발생한다. 이러한 vortex가 발생하기 위해서는 기온 역전층과 5 ~ 13 m/s의 지속적인 바람이 필요하다는 것을 밝히기 위한 많은 연구들이 있었다. 이러한 vortex clouds는 발생 후 주 풍향의 방향을 이동하게 된다. Karman vortex street는 기상학에서 매우 흥미로운 주제이다. 본 연구에서는 LES 난류 모델을 이용하여 Karman vortex street에 관한 해석을 수행하고 수치해석을 통해 얻은 결과와 기상 데이터가 일치함을 보였다. From late autumn to the beginning of the spring, the phenomena of the Karman vortex street have been frequently observed in the wake of Mt. Halla in the Jeju island. Some researches revealed that the necessary conditions for development of these vortex clouds are a strong low-level inversion of temperature and a persistent wind speed of 5 to 13m/s. After the cloud is formed, the vortex clouds are shed from alternating sides of Jeju island and then move downwind with main airflow stream. The Karman vortex street in the wake of the Jeju island was one of the interesting topics in meteorology. In this study, CFD using LES turbulence model was developed to investigate the Karman vortex street and the results of CFD showed a good agreement with weather data.

대학생의 우울정도 : 간호학전공과 타전공대학생을 중심으로

김지연,류나은,이소라,이지희,정윤정,주지연,황인혜 이화여자대학교 간호과학대학 2012 이화간호학회지 Vol.- No.46

Purpose: This study was designed to investigate sample’s general characteristics and compare depression degree among sample’s general characteristics and students’ major(nursing vs. other major). Methods: The subjects consisted of 318 university students. Data was collected by self-reported questionnaires, which were constructed BDI score. Data was analyzed by the SPSS/PC WIN 19.0 program. Results: The depression of nursing students and other major students was not significantly different according to BDI score. Statistically significant difference was identified among sleeping. Conclusion: Specific study focused on the practice time should be done to confirm the depression of nursing major and other majors. Detailed support programs which specifically deal with sleeping should be developed to effectively reduce the harmful effects of individual vulnerability. Therefore, prevention and management system to reduce depression degree for university students is needed.

노인의 영적 간호요구

최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1

This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.

경부외측종괴의 감별진단 및 접근에 대한 고찰

이경호,박현진,강지연,최희원,조용석,김경원,양수남 大韓顎顔面成形再建外科學會 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.6

Most neck masses of specific cause occur in rather predictable locations within typical age groups. This allows a systematic approach to developing a working diagnosis and a differential diagnosis and management plan for the patient presenting with a neck mass. Pathologic masses of the neck will be divided into the following segment: 1. Masses of nonspecific location. 2. Masses in the submandibular region, 3. Masses in the parotid region, 4. Masses in the median-paramedian region, 5. Masses in the lateral neck region. The lateral neck region is the area posterior to the hyolaryngotracheal conduit, below the posterior belly of the digastric muscle and tip of the parotid gland, and extends down to the clavicle. The contents of this region include the large vessels, nerves and greatest number of lymph nodes of the neck, and lymph node metastasis in this region is usually from a primary lesion at the base of the tongue or elsewhere in the oropharynx. Common lateral neck masses include branchial cleft cyst, carotid body tumor, neurogenic tumor, pan-neck infection, cystic hygroma, hemangioma, lipoma and lymphoma. Especially lymphosarcoma. Hodgkin's disease, cystic hygroma, hemangioma and branchial cleft cyst and more common and often manifest in the young patients. Many head and neck disease processes manifest as neck masses. These conditions are treated by surgical excision, except for some inflammatory masses, and often those also must be excised before a diagnosis can be made. We retrospectively reviewed 5-year experiences in the treatment of lateral neck masses at our department to define better clinical characteristics of the masses and the optimal diagnostic and therapeutic approaches to these rare diseases.

객체지향 개념 모델링에서 OCL에 의한 상속성 원리

이지연,최병문,이영환 대전대학교 기초과학연구소 2003 自然科學 Vol.14 No.1

OOA/D에서는 클래스의 일반화와 특수화의 계층구조 사이에서 상속성의 개념이 어디까지 보장되는지 명확한 규칙이 없다. 예를 들어 상위 클래스의 제약조건이 하위 클래스에도 상속되는지, 상위 클래스의 상태 다이어그램이 하위 클래스의 상태 다이어그램에도 상속되는지, 하위 클래스의 행위는 상위 클래스의 행위를 제한시키는 것인지 확장시키는 것인지 등의 문제가 명확하지 않다. 또한 역할과 하위 클래스 설정 문제도 혼동이 일어난다. 본 논문에서는 Liskov의 치환 규칙에 의하여 정적인 면과 동적인 면을 동시에 고려한 상속성 원리를 OCL을 사용하여 정립한다. 이에 따라 역할, 일반화, 특수화, 집합체와 합성에 관한 개념의 가이드라인도 제시한다. There are no explicit rules in OOA/D to indicate whether or not the generalization and specialization hierarchy on a superclass is inherited by its subclasses. For example, we have relevant questions: ls a constraint on a suerclass is inherited by its subclasses? Does subclasses inherit the state diagram of the superclass? Can it refine this state diagram by adding. removing or redefining states, transitions, or events? Can it restrict the behavior of the generalization classes or extend it both? Many methods do not answer these questions. Also concepts of role and subclass are confused in the problem of structure. In this paper, we give an OCL formalization of inheritance by Liskov's Substitution Principle and propose a general guide-lines of concepts of role, generalization, specialization, aggregation, and composition.