Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

기업 윤리프로그램에서 반추가 조직구성원의 비윤리적 행동의 변화와 그 효과의 지속성에 미치는 영향

이지예(Lee, Ji-Ye),장영균(Chang, Young-Kyun),김상수(Kim, Sang-Soo) 한국경영교육학회 2020 경영교육연구 Vol.35 No.4

[연구목적] 많은 기업에서 다양한 기업 윤리프로그램을 실시하고 있지만 그 실효성에 관해서는 많은 의문점이 제기되어 왔다. 교육학 등에서 주로 조명을 받아온 반추(reflection)는 조직의 내 · 외부적 이슈들과 관련하여 올바른 의사결정을 위해 어떻게 행동해야 하는지에 대한 표준을 능동적으로 학습할 수 있는 중요한 과정으로 여겨지고 있다. [연구방법] 본 연구에서는 기업 윤리프로그램에 반추과정을 적용하여 그 효과성을 살펴보고자 하였다. 이를 위해 총 339명을 대상으로 3개의 집단으로 구분하여 실험연구를 수행하였다. 윤리교육을 실시한 후 그 내용에 대한 반추의 과정을 거치는 실험집단1, 윤리교육만을 실시하는 실험집단2, 그리고 윤리교육과 반추의 과정 모두 실시하지 않는 통제집단으로 구분하여 반추의 효과성을 분석하였다. 또한 앞서 참여한 응답자를 대상으로 3주 후 다시 한번 동일한 측정문항으로 조사를 실시하여 그 효과의 지속성을 분석하였다. [연구결과] 실험 결과 윤리교육을 받은 실험집단1과 2에서 통제집단에 비하여 비윤리적 행동경향이 낮게 나타났는데, 특히 반추의 과정을 거친 실험집단1은 그렇지 않은 실험집단2에 비하여 비윤리적 행동 경향이 추가로 더 감소한 것으로 나타났다. 그리고 시간이 지남에 따라 윤리교육으로 인한 효과가 차차 소멸되기는 하지만 반추의 과정을 거칠 경우 그 효과가 더 지속되는 것을 알 수 있었다. [연구의 시사점] 이러한 결과를 통해 윤리프로그램에서 반추의 역할을 조명함과 동시에 기업의 윤리프로그램 효과성 제고에 실무적으로도 기여할 수 있을 것으로 기대한다. [Purpose] Reflection, usually getting more research attention in education, is a necessary component of ethics training because it encourages employees to be more proactive in learning the norms that guide their decision-making regarding various issues. [Methodology] This study designed an experiment by embedding reflection in an ethics training program to examine the effectiveness of such training. A total of 339 participants were allocated to two experimental groups (Group 1 and Group 2) and one control group. [Findings] Findings from the experiment showed that Group 1 and Group 2 are less inclined toward unethical behavior than the control group. Between Group 1 and Group 2, Group 1 that went through the reflection phase shows much less inclination toward unethical behavior than Group 2. The results further indicated that the impact of ethics training gradually diminished over time, but having reflection was found to help sustain the training impact. [Implications] Along with its theoretical contribution that sheds light on the role of reflection in ethics training programs, this study may be worthwhile by providing companies with practical implications that reinforce the effectiveness of ethics training.

상악동저 골이식술 후 임플란트 수에 따른 혼합골 이식재의 수직

박미정,김명래,김지연,김예미,김선종 이화여자대학교 의과학연구소 2012 EMJ (Ewha medical journal) Vol.35 No.1

Objectives: This study was performed to evaluate whether there is the difference of graft height changes in radiographic evaluation in single implants and multiple implants after maxillary sinus floor augmentation with an auto-xenograft mixture. Methods: The study group comprised 29 patients (16 men, 13 women; mean age, 56.5±12.4 years; range, 27 to 87 years) treated at the implant clinic of the Ewha Womans University Mokdong Hospital. They were treated with the sinus floor augmentation of lateral window technique and simultaneous placement of dental implants. Their implants have been evaluated for 42.4±10.5 months. To evaluate the height of grafted material, the real implant length was compared with that of panoramic radiography evaluated by a digital caliper (PACS program), and then the real height of grafted material was assumed. SPSS 19.0 was used in statistical analysis. Wilcoxon singed rank test was utilized for P value and Mann-Whitney for statistical significance in the changes of grafted height between single implants and multiple implants. The level of statistical significance was defined as P<0.001. Results: The evaluated graft height in single implants' group decreased to 0.60±0.54 mm at the time of delivery (after 7.5 months, P=0.001), in multi-unit implants' group to 0.96±0.84 mm (after 6.5 months, P<0.001). The evaluated graft height in single implants' group decreased to 1.11±0.75 mm at the last follow-up (after 43.5 months, P=0.001), in multi-unit implants' group to 1.52±0.99 mm (after 41.8 months, P<0.001). Mann-Whitney test was used for the difference between single implants and multi-unit implants. There was no difference in two groups at the time of delivery and the last follow-up (P>0.001). Conclusion: There was no statistical significance of radiographic graft height after maxillary sinus floor augmentation between single implants and multiple implants.

만성 견비통에 대한 동씨침 치료의 무작위 대조군의 임상 연구

김찬영,권나현,신예지,남동우,김건형,김종인,최도영,이윤호,이재동 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

Objectives : To observe the effect of acupuncture treatment in chronic shoulder pain patients. Methods : 36 voluntary patients were randomly assigned to an acupuncture treatment group(E GrouP, n=18) and a control group(C GrouP, n=18). The E Group patients received acupuncture treatment on LI_(15), TE_(14), GB_(21) and Master Dong's acupuncture points, Shin-gwan and Gyun-joong, twice a week for four weeks. The C Group patients received no treatment. All patients in both groups were instructed to practise self exercise in their daily lives. Evaluations were made at baseline and after four weeks of study. The Constant Shoulder Assessment(CSA), Shoulder Pain and Disability Index(SPADI) and the patient's subjective pain was measured by Visual Analogue Scale(VAS). The obtained data was analyzed. Results '. The E Group showed significant(p<0.05) improvement in CSA, SPADI and VAS after four weeks of treatment. The C Group showed significant(p<0.05) improvement in CSA, but the change of SPADI and VAS was insignificant(P>0.05). CSA and SPADI of E Group significantly(p<0.05) improved compared to the C GrouP, but the difference of VAS change in the two groups was insignificant(p>0.05). Conclusions : Four weeks of acupuncture treatment significantly improved CSA, SPADI and VAS. The improvement of CSA and SPADI was significant(p<0.05) compared to untreated patients.

근피로 후 세 가지 회복 방법

이예영,김지은,서예은,정다연,강현주 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Muscle fatigue is a symptom that decreases your muscles’ ability to perform over time. It can be associated with a state of exhaustion, often following excessive sports activity or exercise. While exercise is a common cause of muscle fatigue, this symptom can be the result of other health conditions. Muscle fatigue caused by excessive activity limits activities of daily living and difficult to maintain a stable posture. Therefore, the purpose of this study was to investigate the effect of stretching, icing, and sports massage on muscle fatigue recovery.

충북지역 대학생들의 음주정도와 식생활지침 이행도와의 관계

김기남, 김지현, 정예지 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

The purpose of this study was to investigate the association of alcohol drinking and the practices of dietary guidelines among university students in Chungbuk. Questionnaires were distributed to 351 university students and 348 (203 males and 145 females) were collected from May 21 to June 28, 2012. "Soju" was the most frequently consumed alcohol by university students. As for drinking amount, 32.7% of the students drank "ten glasses of soju or more" at one time. The frequency and the amount of drinking were significantly greater in male students than female students. While many students practiced "handling food hygienically when cooking" and "choosing clean ones when purchasing food or dining out", less students practiced "controlling energy consumption according to the activity level" and "removing fat when consuming meat." Those who drank four times per week or more responded a lower rate of practice than those who drank less frequently. As for drinking motive, those who drank "habitually" also recorded a lower rate of practice than those who drank "to fit in the mood." In conclusion, alcohol drinking had negative influences on the dietary practice of university students. Campaigns or nutrition education should be designed to help to establish a healthy drinking culture in university

합병증을 동반한 편두통 1례

김예성,김지선,정성해,김재문 대한두통연구회 2003 두통 Vol.4 No.2

임부의 연령에 따른 보완대체요법에 관한 인식 태도 경험 비교 연구

김연진,김지연,노경민,신혜리,유예지,이지민,장희조,정아인,최소정,강숙정,이향연,민혜영 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Purpose: This study aims to test if the age of pregnant women affects the perception, experience, and attitude towards complementary alternative therapy (CAT), by investigating the perception, experience, and attitude of pregnant women under the age of 35, and above 35 (Advanced Maternal Age). The study will 1) find the general characteristics of pregnant women under and over the age of 35 2) compare the perception, behavior, and experience of pregnant women under and over the age of 35, and 3) find the comparison of the perception, behavior, and experience according to the general characteristics of pregnant women under and over the age of 35. Method: The subjects of this study are composed of 44 women under the age of 35 and 44 women above the age of 35, totaling 88 pregnant women visiting midwiferies, obstetrics and gynecology, and baby fairs in Seoul and Gyeong-Gi area. Data for this study was collected from November 28, 2015 to January 6, 2016 for 40 days. The collected data analyzed mean, standard deviation, t test, ANOVA, and chi-square by using SPSS 23.0. Result Results show that there is a significant difference in the perception, attitude, and experience of the two-sample groups in the use of CAT. The question of “what is most necessary when applying CAT in a hospital clinical environment” for the perception criteria came out with the results of t=9.980, p<.05. For the attitude, results showed that the answers of pregnant women under the age of 35 displayed a more positive attitude towards CAT. (t=2.421, p<.05) Lastly, in the experience section, pregnant women under the age of 35 showed more CAT use than the other sample group. Conclusion Results have shown that there is a difference in the perception, behavior, and experience between the two sample groups. Therefore further research on the efficacy and the adverse effects of CAT needs to be done.

중증 화상 환자에서 영양지원

박지영,목혜숙,신현선,노순예,김애심,백금선,김도헌,허준,전욱 대한화상학회 2003 대한화상학회지 Vol.6 No.2

정신지체 특수학교 직업과 교육과정 분석 : 장애인 고용 실태와의 비교를 바탕으로

임예지,김호연 한국장애인고용공단 고용개발원 2012 장애와 고용 Vol.22 No.2

This study was intended to analyze the vocational education curriculum based on the job status of people with disabilities. For this purpose, secondary data from statistical data of 'survey of the disabled employment reality - in-depth survey' implemented by Korea Employment Agency for the Disabled were used to reveal the job status of people with disabilities. In addition, vocational education curriculum of three special schools was analyzed. In this study, following issues are suggested. First, vocational education for students with intellectual disabilities should focus the adaption ability at working sites and provide field oriented education which is for enhancing job skills. Second, by emphasizing working site-based education, an opportunity of being able to apply occupational skill and knowledge obtained with an aim of responsible duty performance and achievement of duty goals that are required at working sites should be provided diversly. Third, in order to share an information for man-recruit as well as job-seeking between school and the job sites efficiently, a communication channel is required in a governmental perspective. 본 연구는 장애인 근로자의 직업의 실태와 직업현장에서 요구하는 요구사항을 분석하고, 특수학교에서 운영되고 있는 기본교육과정 직업교과와 전공과 교육과정을 비교하여 연계성을 찾고 이를 통해 문제점을 도출하여 정신지체학생의 직업교육의 방안을 제시하고자 하는데 그 목적이 있다. 본 연구를 위하여 한국고용공단에서 실시한 장애인 고용실태의 자료의 일부를 활용하였고, 3개의 정신지체 특수학교의 직업교육 및 전공과 교육과정이 분석되었다. 연구 결과를 종합하여 보면 현재 운영 되고 있는 정신지체 특수학교 직업 교육과정은 학교 선택 직종과 취업 현장의 직무는 연계성은 있으나 취업 전형, 사업체에서 필요로 하는 신체적 기능, 직무 능력, 업무 수행능력 등과 관련된 교육 내용이 적은 것으로 나타났다. 따라서 정신지체 직업 교육은 정신지체인의 직업 현장 적응력을 높이고 사업체의 장애인 근로자에 대한 만족을 높일 수 있도록 직업 영역과 제재를 포함하고 관련된 지식과 기능을 늘릴 수 있도록 시수 등을 보강하는 노력을 해야 할 것이다. 또한 학교와 사업체 간의 구인과 구직에 관한 정보를 효율적으로 공유할 수 있도록 정부적인 측면에서 의사소통의 장이 필요하다. 끝으로 연구의 제한점과 후속연구에 대한 제언이 포함되었다.