Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

심혈관계 질환으로 관상질환 집중치료실에 입원한 환자들의 혜후 측정을 위한 APACHEⅢ점수 체계의 임상적 유용성

이공섭,이창선,최현주,홍종서,강지향,김도형,이민영,오인균,김병엽,김상억,김학찬,한승혜,이재용,지영우,안경주 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

Sarcomatoid Carcinoma Arising from Mature Cystic Teratoma

Ho-Chang Lee1, Seung-Myoung Son, Yong-Moon Lee, Ji Hae Koo, Song-Yi Choi, Ok-Jun Lee, Eun-Hwan Jeong 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2

Malignant transformation of mature cystic teratoma (MCT) is rare. Sarcomatoid carcinoma is a neoplasm comprising malignant mesenchymal cells and a conventional carcinomatous area. Here, we report on a case of sarcomatoid carcinoma arising from an MCT in the left ovary of a 45-year-old female. A unilocular cyst consistent with MCT was observed; however, a nodule within the cyst was confirmed from the resected ovary. Microscopically, the nodule showed both squamous cell carcinoma and pleomorphic sarcomatous components admixing with each other. Lining epithelial cells at the periphery of the main tumor showed squamous metaplasia. When a sarcomatous component is observed in the ovary tumor, it is important to find a squamous cell component, either benign or malignant.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

직무 스트레스 평가를 위한 측정도구 개발 및 표준화 연구(Ⅱ) : 인구통계학적 변인의 영향 및 규준 수립 Demographic Variables and Norm

이동수,김지혜,한우상,우종민,강동우,고영건,연병길,김이영 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: 본 연구에서는 Osipow와 Spokane(1992)의 직무 스트레스 모형에 기초한`직무 스트레스 검사(Occupational Stress Inventory)`를 표준화하고 규준을 작성하기 위해서 성별, 연령, 직업기간, 직종 등의 인구통계학적 변인에 따라서 직무 스트레스의 경험이 어떤 차이가 있는지를 확인하기 위한 분석을 실시하고, 이에 따라 규준을 작성하였다. 방 법: 한국직업사전 직업분류에 따라서 7개 직종군의 805명(남성 76.5%, 여성 23.5%)에게 직무 스트레스 검사를 실시하였다. 성별, 연령, 연봉, 직업기간, 일반직종, 직급 차이, 일반직/특수직, 사무직/근로직에 따라 직무 스트레스의 경험과 반응이 어떻게 달라지는지를 살펴보았다. 이후, 이러한 결과에 기초하여 남성, 여성 집단 각각의 규준을 작성하였다. 결 과: 1) 남성과 여성은 직무 스트레스의 경험, 스트레스에 대한 반응, 대처 행동에서 서로 차이가 있었다. 전반적으로 여성은 직무 자체의 특성에 따른 스트레스 수준이 높았는데, 남성집단에 비해 역할 불충분(RI), 역할 모호성(RA), 사회적 지지(SS), 직무 긴장(VS) 척도의 수준이 높았으며, 남성은 여성에 비해 역할 과부하(RO), 책임감(R) 척도의 수준이 높았다. 2) 다음으로 연속변인으로 측정한 연령, 연봉, 직업기간과 직무 스트레스와의 상관분석을 실시한 결과, 일반적으로 연령이 많고, 연봉이 높고, 직업에 종사한 기간이 길수록 직무로 인한 스트레스와 그에 따른 문제가 적고, 적절한 대처자원을 가지고 있었다. 3) 일반적인 직종에 따라서 직무 스트레스의 경험에 차이가 있는지를 확인하기 위해서 연령, 연봉, 직업기간을 통제한 공변량분석(ANCOVA)을 실시하였는데, 남성집단, 여성집단 모두에서 일반적인 사무직종 내에서는 직무 스트레스의 경험이 유사한 것으로 나타났으며, 동일한 직종 내에서 직급에 따른 고유한 효과는 없었다. 다음으로 일반 사무직종 이외에 경비회사 직원, 직업군인(장교)과 같은 특수 직종에 종사하는 사람들의 직무스트레스 경험이 일반 회사원과 차이가 있는지를 확인하기 위해서 공변량분석을 실시하였을 때, 직종간 유의미한 차이가 존재하였으며, 일반 사무직과 기능 근로직에 대한 비교에서도 직종에 따른 차이가 유의미하게 나타났다. 이러한 결과를 종합하여 보면, 일반 사무직종 내에서 직종에 따른 직무 스트레스 경험의 차이는 적지만, 특수직종과 기능직을 비교하였을 때는 직종에 따라서 전반적으로 유의미한 차이가 있음을 확인할 수 있었다. 마지막으로 앞선 분석결과에서 가장 의미 있는 차이가 나타난 변인인 성별을 기준으로 한국판 직무 스트레스 검사의 규준을 작성하였다. 결 론: 직무 스트레스에 대한 개인의 경험과 반응은 성별, 연령, 연봉, 직업기간, 직종, 직급 등의 여러 인구통계학적인 변인에 따라 서로 유의미한 차이가 있는 것으로 나타났다. 이러한 결과는 한 개인의 스트레스 경험과 반응을 객관적으로 이해하기 위해서는 그 개인의 인구 통계학적인 특성에 대한 폭넓은 탐색이 필요하다는 점을 시사한다. 한편, 본 연구를 통해서 개발된 규준을 이용하여, 개인의 스트레스 경험을 상대적으로 비교할 수 있으며, 검사의 결과를 적절한 임상적 개입을 위한 지표로 사용할 수 있을 것이다. Objectives: This study examined the effects of demographic variables on the job stress experience and established the norms of the Korean version of Occupational Stress Inventory. Methods: The normative group consisted of 805 adult workers who represent seven major job class in Korea. K-OSI were administered to normative group and data were analyzed by subjects demographic variables. Results: To explore the effects of demographic variables on K-OSI, we considered several factors such as age, sex, duration of employment, job class, job position and so on. The results were as follows : 1) We found significant sex difference in K-OSI. Compared with men, women showed higher scores in Role Insufficiency, Role Ambiguity, Social Support, Vocational Strain Scales and men showed higher scores in Role Overload, Responsibility Scales. 2) Generally, a person who was older, earned higher salary, more duration of employment experienced job stress and had more coping resources. 3) In general, white-collar workers, job classes and job position had little effects on job stress. But other workers such as security guards, military officers were different from general white-collar workers in K-OSI. Conclusions: Because men and women showed significant difference in K-OSI, we constructed norms according to sex. K-OSI would be an useful instrument to identify one`s job stress experience and develop intervention plan.

정신분열병환자의 뇌량에 대한 신경병리적 고찰 : 확산텐서영상(Diffusion Tensor Image)기법의 적용

이경진,신용욱,하태현,이종민,김인영,김선일,문원진,정은철,박해정,오정은,박지영,강경훈,권기원,권준수 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.4

Objectives : Diffusion tensor imaging (DTI) is a new technique that can be used to visualize and measure the diffusion of water in brain tissue. It is particularly useful for evaluating white matter abnormalities. In this paper, we investigated the neuropathology of the corpus callosum in patients with schizophrenia through the new methods, diffusion tensor imaging. Methods : Diffuse tensor imaging was performed in 19 schizophrenic patients and 26 healthy controls. One complementary measure, fractional anisotropy (FA), which is considered to be sensitive indices of axonal integrity, was obtained from regions of interest in the five areas of the corpus callosum. Neuropsychological measurement also underwent for measurement of frontal lobe function in two groups. Results : There were no significant differences of FA in the DTI measures for either the schizophrenic patients compared with controls. FA was significantly decreased in women compared with men. Neuropsychological measurements such as Wisconsin card sorting test were correlated with FA of patients with schizophrenia. Conclusion : FA measures revealed no differences between schizophrenia and controls. The findings suggest that the structural integrity of white matter in patient with schizophrenia was not disrupted. The results are discussed in relation to previous studies reporting positive and negative findings. It is concluded that further studies using DTI in larger samples, improved and standardized methods of data acquisition and analysis are needed.

전화상담이 치매노인 간호제공자의 소진경험, 우울, 삶의 질, 지각된 건강상태에 미치는 영향

이해정,김기련,서지민 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: The purpose of this study is to identify the effects of telephonic counseling on burnout, depression, life satisfaction, and perceived physical health among family caregivers of older adults with dementia. Method: Subjects were randomly assigned into telephonic counseling group (n=21) and the comparison group (n=32). A weekly telephone counseling was conducted by research assistants for 12 weeks. T-test were used to answer the research questions. Result: 1) There were no significant differences between the two groups on the level of burnout, depression, life satisfaction, and perceived physical health after telephone counseling. 2) Spouse caregivers under the telephone counseling tended to report higher perceived physical health than comparison group at the post-test (t=-1.88, p=.08). Spouse caregivers under the telephone counseling tended to report higher emotional exhaustion and lower feeling of self achievement. 3) Daughter-in-law caregivers under telephone counseling showed increased feeling of self achievement, improved physical health condition, and decreased depression. Conclusion : This study showed potential effects of the problem-solving telephone counseling to improve perceived physical health and to reduce the level of burnout and depression. The findings suggest the necessity of screening most vulnerable subgroups of caregivers to increase the effectiveness of nursing intervention such as telephone counseling.

신체화장애 환자의 스트레스 대처방식 및 심리적 특성

이주연,이무석,윤진상,양종철,문지웅,정해원,은성종 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.6

Objectives : The purpose of this study was to investigate the stress coping strategies and psychological charactehstics, such as combined psychopathology and tendency of symptom interpretation, in patients with somatization disorder. Methods : Thirty patients meeting the criteria of DSM-IV somatization disorder were compared with thirty controls. We evaluated the subjects using Symptom Checklist-90-Revised (SCL-90-R), Somato-Sensory Amplification Scale (SSAS), Symptom Interpretation Questionnaire (SIQ), and The Ways of Stress Coping Questionnaire (SCQ). Independent t-test and Pearson correlation analysis were used. Results : From the results of SCL-90-R subscales, the scores of somatization, obsession-compulsion, depression, anxiety, and psychoticism were significantly higher in patients with somatization disorder than nonnal controls. Somatization disorder patients had greater amplification of physical sensation in SSAS and significantly higher score in physical interpretation of SIQ compared with psychological or environmental interpretation, In the SCQ of somatization disorder patients, we observed generallylower levels of total coping scores than the control group and significant positive correlation between passive coping style of SCQ and psychological interpretation of SIQ. Conclusion : These results show that patients with somatization disorder have various psychopathology, greater amplification of physical sensation, physical interpretation tendency of symptoms, and insufficient copying strategy.

직무스트레스가 정신과적 증상에 미치는 영향 : 성별에 따른 자존감의 매개 효과를 중심으로

이동수,김지혜 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.2

연구목적: 본 연구는 직무스트레스가 정신과적 증상에 미치는 영향과 이에 있어서 남녀에 따라 자존감의 매개 효과에 차이가 있음을 알아보는 것을 목적으로 하였다. 방법: 종합병원과 벤처기업 등의 회사에서 일반사무직으로 근무하는 남성 226명과 여성 119명을 대상으로 직무스트레스 검사(OSI)의 일부와 BDI(Beck's Depression Index), STAI(State-Trait Anxiety In-ventory)의 state anxiety inventory, SCL-90-R(Symptom Check List-90-Revised)와 RSES(Rosenberg's Self-Esteem Scale)를 실시하였다. 변인들간의 관계를 알아보기 위하여 상관 분석을 실시하였으며, 직무스트레스가 정신과적 증상에 미치는 영향과 여기에 미치는 자존감의 매개 효과의 성별에 따른 차이를 알아보기 위해서 남녀 각각에 대해서 변량분석을 실시하였다. 결과: 직장 남성의 경우 역할 경계 변인과 역할 불충분 변인이, 직작 여성의 경우에는 물리적 환경과 역할 과부하 변인이 우울, 불안, 신체화 증상에 영향을 미치는 주요 변인으로 나타났으며, 자존감은 남성의 경우에만 직무스트레스와 상기 증상에 매개 변인 효과를 갖는 것으로 나타났다. 결론: 직장 남성의 경우에는 각 개인의 역할 경계를 명확히 하고 주어진 역할에 적절한 직무를 부과하며 개인의 능력 수준에 어울리는 충분한 수준의 직무를 부과하는 것이 정신과적 증상을 예방할 수 있음을 시사한다. 한편 여성의 경우에는 직장 내에서 유해한 작업환경에 노출되는 정도를 줄여 주고 각 개인의 역량과 여건을 초과하지 않는 정도의 직무를 부과하는 것이 정신과적 증상을 줄일 수 있는 방법이 될 것으로 생각된다. 또한 남성의 경우에는 자존감의 수준이 높으면 직무 스트레스 변인이 정신과적 증상에 미치는 영향을 감소시키는 효과가 있는 것으로 나타났으므로 자신에 대해서 일반적으로 긍정적으로 느끼는 정도인 자존감을 고취시키는 것이 직무스트레스의 부정적인 효과를 줄임으로써 정신과적 증상을 사전에 예방할 수 있는 방법이 될 수 있음을 알 수 있다. Objective: The purpose of this study was to identify the mediation effect of gender difference and self-esteem level between job stress and psychiatric symptom. Method: Male and female subjects participated in this study and filled out the Korean version of Job Stress Inventory(K-OSI), Beck's Depression Index(BDI), State-Trait Anxiety Inventory(STAI), Symptom Check List-90-Revised(SCL-90-R) and Rosenberg's Self-Esteem Scale(RSES). To explore the relationships between job stress and psychiatric symptom, a series of correlational analysis and ANOVAs were conducted to identify mediation effects self esteem level to job stress experience. Results: In males, the perceived job stress level of role boundary scale was positively correlated with depressive, anxiety, psychosomatic symptoms. And role insufficiency was positively correlated with depressive and anxiety symptoms, too. In addition, the interaction effect of self-esteem was significant and the interaction effect of self-esteem was significant. It means high level of self-esteem had buffering effect of job stress on psychiatric symptoms. In females, the perceived job stress level of physical environment scale was positively correlated with depressive symptoms and role overload was positively correlated with psychosomatic symptoms. There was no significant interaction effect of self-esteem identified. Conclusions: The perceived job stresses related to role boundary and role insufficiency were important factors which result in psychiatric symptoms in males, while physical environment and role overload were important factors in females. In addition, high self esteem had buffering effects of job stresses on psychiatric symptoms, only in males not in females