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( Ok-hee Kim ),( Hyojung Kim ),( Jinku Kang ),( Dongki Yang ),( Yu-hoi Kang ),( Dae Ho Lee ),( Gi Jeong Cheon ),( Sang Chul Park ),( Byung-chul Oh ) 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.1
Accumulation of tissue macrophages is a significant cha-racteristic of disease-associated chronic inflammation, and facilitates the progression of disease pathology. However, the functional roles of these bone marrow-derived macrophages (BMDMs) in aging are unclear. Here, we identified age-dependent macrophage accumulation in the bone marrow, showing that aging significantly increases the number of M1 macrophages and impairs polarization of BMDMs. We found that age-related dysregulation of BMDMs is associated with abnormal overexpression of the anti-inflammatory interleukin-10. BMDM dysregulation in aging impairs the expression levels of pro-inflammatory cytokines and genes involved in B-cell maturation and activation. Phagocytosis of apoptotic Jurkat cells by BMDMs was reduced because of low expression of phagocytic receptor CD14, indicating that increased apoptotic cells may result from defective phagocytosis of apoptotic cells in the BM of aged mice. Therefore, CD14 may represent a promising target for preventing BMDM dysregulation, and macrophage accumulation may provide diagnostic and thera-peutic clues. [BMB Reports 2017; 50(1): 43-48]
Molecular Cloning and Expression of DMRT Gene in Protogynous Wrasse, Halichoeres tenuispinis
Jeong Hyung-Bok,Park Ji-Gweon,Park Jin-Young,Jin Young-Jun,Yang Myung-Cheon,Hyun Kyung-Man,Kim Gi-Ok,Kim Se-Jae 한국발생생물학회 2003 한국발생생물학회 학술발표대회 Vol.2003 No.1
The sex differentiation of fishes occurs under the control of genetic and various environmental factors. DM-domain containing genes are novel zinc finger transcription factors and play key roles in sex determination. In order to isolate the wrasse DMRT (wDMRT) cDNA from the protogynous wrasse (Halichoeres tenuispinnis), the wrasse testis cDNA library was screened using the P-labeled PCR products, which were amplified with the degenerate primers from conserved DM-domain regions of several DMRT genes. Among a few positives obtained through screening, the full length wDMRT cDNA of 2.9kb size encoding a predicted 300 amino acid residues was isolated. The sequence analysis exhibited 60%, 43% sequence identity with rainbow trout and tilapia DMRT1, respectively. RT-PCR assay showed that wDMRT was expressed specifically in male testis. Also, wDMRT gene was strongly expressed in May during reproductive season, when the reproductivity of wrasse is most active. This results suggested that wDMRT gene function in testis differentiation The conserved DM-domain regions were amplified using PCR from DMRT genes of several species among Labridae, and their sequences were determined. The sequence of DM-domain region of Halichoeres. tenuispinis was identical to those of Pseudolabrus japonicus, Pteragogus flagellifera, and showed 94% identity with that of Halichoeres poecioptrerus.
Yang, Angel,Park, Jong-Hyouk,Abd El-Aty, A.M.,Choi, Jeong-Heui,Oh, Jae-Ho,Do, Jung-Ah,Kwon, Kisung,Shim, Ki-Hoon,Choi, Ok-Ja,Shim, Jae-Han Elsevier 2012 FOOD CONTROL Vol.28 No.1
<P><B>Abstract</B></P><P>The principal objective of this study was to investigate the effect of household processing, including washing and cooking on pesticide residue levels in various food samples. For this study, 31 food materials were selected and 44 pesticide residues were monitored using the “quick, easy, cheap, effective, rugged, and safe” QuEChERS extraction-based and liquid chromatography–tandem mass spectrometry (LC–MS/MS) methods. Eight pesticides, including acetamiprid, azoxystrobin, fenobucarb, fosthiazate, iprobenfos, lufenuron, propiconazole, and trifloxystrobin were detected in nine food samples including colored rice, glutinous rice (white rice), glutinous rice (unpolished rice), green chili, ginger, butterbur, chinamul, spinach, and perilla leaf. Results indicated that residue levels in positive food commodities declined substantially following washing and cooking. However, the residual level of acetamiprid increased in green chilis after boiling and stir-frying. In sum, household processing (washing and cooking) tended to substantially reduce or eliminate pesticide residues in a synergistic manner. These applications are necessary to protect consumers from the negative health effects of pesticide residues detected in food commodities.</P> <P><B>Highlights</B></P><P>► The effects of household processing on pesticide residue levels were investigated. ► Residue levels were substantially declined following washing and cooking. ► The household processing tend to remove the residues in a synergistic manner.</P>
Jeong Hoon Yang,Joo Myung Lee,Taek Kyu Park,Young Bin Song,Joo-Yong Hahn,Jin-Ho Choi,Seung-Hyuk Choi,Cheol Woong Yu,Woo Jung Chun,Ju Hyeon Oh,Bon-Kwon Koo,Jin-Ok Jeong,Hyo-Soo Kim,Hyeon-Cheol Gwon 대한심장학회 2019 Korean Circulation Journal Vol.49 No.6
Background and ObjectivesThere are limited data regarding the clinical efficacy of the proximal optimization technique (POT) in the treatment of coronary bifurcation lesions. We investigated the influence of POT on the clinical outcomes of patients with coronary bifurcation lesions. MethodsWe enrolled a total of 1,191 patients with a bifurcation lesion with a side branch (SB) diameter ≥2.5 mm treated with a drug-eluting stent from 18 centers between January 2003 and December 2009. The primary outcome was major adverse cardiac events (MACEs: cardiac death, myocardial infarction or target lesion revascularization [TLR]). We performed one-to-many (1:N) propensity score matching with non-fixed matching ratio. ResultsPOT was performed in 252 patients. During follow-up (median 37 months), the incidence of MACE was lower in the POT group than it was in the non-POT group (adjusted hazard ratio, 0.43; 95% confidence interval [CI], 0.24–0.79; p=0.006). After propensity score matching, these were 0.34; 95% CI, 0.17–0.69; p=0.003 for MACE and 0.37; 95% CI, 0.17–0.78; p=0.01 for TLR. The use of POT was associated with significantly lower TLR in patients treated without kissing ballooning, but was not in those who underwent kissing ballooning (p for interaction=0.03). ConclusionsIn coronary bifurcation lesions with a large SB, POT may be beneficial to improve long-term clinical outcome, particularly in patients treated without kissing ballooning during the procedure. Trial RegistrationClinicalTrials.gov Identifier: NCT01642992
Yang, Taek Ho,Kim, Tae Wan,Kang, Hye Ok,Lee, Sang-Hyun,Lee, Eun Jeong,Lim, Sung-Chul,Oh, Sun Ok,Song, Ae-Jin,Park, Si Jae,Lee, Sang Yup Wiley Subscription Services, Inc., A Wiley Company 2010 Biotechnology and Bioengineering Vol.105 No.1
<P>For the synthesis of polylactic acid (PLA) and its copolymers by one-step fermentation process, heterologous pathways involving Clostridium propionicum propionate CoA transferase (Pct<SUB>Cp</SUB>) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1<SUB>Ps6-19</SUB>) were introduced into Escherichia coli for the generation of lactyl-CoA endogenously and incorporation of lactyl-CoA into the polymer, respectively. Since the wild-type PhaC1<SUB>Ps6-19</SUB> did not efficiently accept lactyl-CoA as a substrate, site directed mutagenesis as well as saturation mutagenesis were performed to improve the enzyme. The wild-type Pct<SUB>Cp</SUB> was not able to efficiently convert lactate to lactyl-CoA and was found to exert inhibitory effect on cell growth, random mutagenesis by error-prone PCR was carried out. By employing engineered PhaC1<SUB>Ps6-19</SUB> and Pct<SUB>Cp</SUB>, poly(3-hydroxybutyrate-co-lactate), P(3HB-co-LA), containing 20–49 mol% lactate could be produced up to 62 wt% from glucose and 3HB. By controlling the 3HB concentration in the medium, PLA homopolymer and P(3HB-co-LA) containing lactate as a major monomer unit could be synthesized. Also, P(3HB-co-LA) copolymers containing various lactate fractions could be produced from glucose alone by introducing the Cupriavidus necator β-ketothiolase and acetoacetyl-CoA reductase genes. Fed-batch cultures were performed to produce P(3HB-co-LA) copolymers having 9–64 mol% of lactate, and their molecular weights, thermal properties, and melt flow properties were determined. Biotechnol. Bioeng. 2010; 105: 150–160. © 2009 Wiley Periodicals, Inc.</P>
Fabrication of a Thermopneumatic Valveless Micropump with Multi-Stacked PDMS Layers
Jeong, Ok-Chan,Jeong, Dae-Jung,Yang, Sang-Sik The Korean Institute of Electrical Engineers 2004 KIEE International Transactions on Electrophysics Vol.4C No.4
In this paper, a thermopneumatic PMDS (polydimethlysiloxane) micropump with nozzle/diffuser elements is presented. The micropump is composed of nozzle/diffuser elements as dynamic valves, an actuator consisting of a circular PDMS diaphragm and a Cr/Au heater on a glass substrate. Four PDMS layers are used for fabrication of an actuator chamber, actuator diaphragm by a spin coating process, spacer layer, and nozzle/diffuser by the SU-8 molding process. The radius and thickness of the actuator diaphragm is 2 mm and 30 ${\mu}{\textrm}{m}$, respectively. The length and the conical angle of the nozzle/diffuser elements are 3.5 mm and 20$^{\circ}$, respectively. The actuator diaphragm is driven by the air cavity pressure variation caused by ohmic heating and natural cooling. The flow rate of the micropump in the frequency domain is measured for various duty cycles of the square wave input voltage. When the square wave input voltage of 5 V DC is applied to the heater, the maximum flow rate of the micropump is 44.6 ${mu}ell$/min at 100 Hz with a duty ratio of 80% under the zero pressure difference.
Affinity Maturation of an Anti-Hepatitis B Virus PreS1 Humanized Antibody by Phage Display
Yang, Gi-Hyeok,Yoon, Sun-Ok,Jang, Myung-Hee,Hong, Hyo-Jeong The Microbiological Society of Korea 2007 The journal of microbiology Vol.45 No.6
In a previous study we generated an anti-Hepatitis B Virus (HBV) preS1 humanized antibody (HzKR127) that showed in vivo HBV-neutralizing activity in chimpanzees. However, the antigen-binding affinity of the humanized antibody may not be sufficient for clinical use and thus affinity maturation is required for better therapeutic efficacy. In this study, phage display technique was employed to increase the affinity of HzKR127. All six amino acid residues (Glu95-Tyr96-Asp97-Glu98-Ala99-Tyr100) in the heavy (H) chain complementary-determining region 3 (HCDR3) of HzKR127 were randomized and phage-displayed single chain Fv (scFv) library was constructed. After three rounds of panning, 12 different clones exhibiting higher antigen-binding activity than the wild type ScFv were selected and their antigen-binding specificity for the preS1 confirmed. Subsequently, five ScFv clones were converted to whole IgG and subjected to affinity determination. The results showed that two clones (B3 and A19) exhibited an approximately 6 fold higher affinities than that of HzKR127. The affinity-matured humanized antibodies may be useful in anti-HBV immunotherapy.