신장세포암에서 WT1과 IGF2 유전자의 발현

이정호,김응석,김용섭,장성익,윤환중 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

IGF2 유전자는 제 11번 염색체 단완 (11p15.5)에 위치해 있으며 아버지로부터 유전된 대립형질만 기능을 하는 genomic imprinting되는 유전자이다. 이 유전자는 정상세포에서는 세포증식을 촉진시키는 작용을 하고 몇가지 암에서는 LOI에 기인한 과발현이 있는 것으로 알려져 있다. 한편 WT1 유전자는 제 11번 염색체 단완 (11p13)에 위치해 있으며 어머니로부터 유전된 대립형질만 기능을 하는 즉 IGF2 유전자와는 정반대의 genomic imprinting 되는 유전자이다. 이 유전자는 비뇨생식기관의 발생에 매우 중요한 유전자이며 암화과정에서는 암억제 유전자로서 기능한다. 소아신장에서 발생되는 Wilms 종양에서는 IGF2 유전자와 WT1 유전자의 고장이 주원인으로 생각하고 있다. 이에 근거하여 성인에서 발생되는 신장세포암에서도 Wilms 종양에서의 결과와 같을 것인지의 여부를 알기 위하여 12명의 신장세포암 환자 ( 남자 6명, 여자 6명)로부터 얻은 조직을 이용하여 이들 유전자의 발현정도를 조사하였다. 환자의 연령은 남자에서는 43세에서 56세까지였고 여자에서는 23세에서 61세까지였다. 신장세포암에서 IGF2 유전자와 WT1 유전자의 발현정도는 다같이 성별이나 연령과는 무관하였다. IGF2 유전자는 50%에서 발현되었으며, 그 발현정도는 저발현에서부터 중등도 그리고 과발현까지 다양했으며 주로 신세뇨관 부위에 있는 암세포에서 발현되었다. WT1은 56%에서 발현되었으며 과발현과 중등도 발현이 대부분이었다. IGF2 유전저의 발현과 WT1 유전자의 발현은 각 예에서 상이하게 나타나서 두 유전자간의 작용은 일치하지 않았다. IGF2 gene which was located on chromosome 11p15.5 is expressed by the paternal allele. It stimulates the cell proliferation in normal cells, however, it is over expressed in some cancers due to LOI (loss of genomic imprinting). In contrast, WT1 gene which was located on chromosome 11p13 shows maternal specific monoallelic expression. It is a very much important gene in developing urogenital system, besides, it acts as tumor suppressor gene in some cancers. IGF2 and WT1 genes are the main causative genes in Wilms tumor which is developed in pediatric kidney. To understand the status of IGF2 and WT1 expressions in renal cell carcinoma which is developed in adult life, tissues from 12 cases patients (male 6 cases ; female 6 cases) were detected by immunohistochemistry with antibodies from IGF2 and WT1 genes. To distinguish the different ages between Wilms tumor and renal cell carcinoma, the patients age in renal cell carcinoma was ranged in 43-56 years old in man and 23-61 years old in female. No direct correlation was, in general, between gene expression of IGF2 and WT1 and age or sex of patients in renal cell carcinoma. Expression of IGF2 gene revealed six of twelve (560%) and expression of WT1 gene showed seven of twelve (56%) in renal cell carcinoma, respectively. The status of IGF2 gene expression was varied from low to high expression. In contrast, the status of WT1 gene expression was higher than in IGF2 gene expression. However, there was no apparent correlation between expressions in each cases. In conclusion, IGF2 and WT1 genes are very important in carcinogenesis of renal cell carcinoma, however interaction between two genes was obscure.

랫드에서 단일시험법을 이용한 반하 물추출물의 생식독성시험

이기남,유욱준,백인정,연정민,남상섭,이범준,남상윤,김윤배,강현규,안병우,김대중,황방연,노재섭,홍정섭,성하정,김순선,조대현,윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

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췌장의 악성 글루카곤종: 1예 보고

윤지영,정종길<SUP>1<.SUP>,정웅길<SUP>2<.SUP>,박미옥<SUP>3<.SUP>,Ji Young Yun,Jong Gill Jeong,M.D.<SUP>1<.SUP>,Ung Gill Jeong,M.D.<SUP>2 <.SUP>and Mi-Ok Park,M.D.<SUP>3<.SUP> 대한갑상선-내분비외과학회 2002 The Koreran journal of Endocrine Surgery Vol.2 No.2

Glucagonomas are rare pancreatic tumors of islet ahpha-2 cells. Less than 430 cases have been reported worldwide and 210 cases are malignant tumors. In generally, the tumors typically present with a characteristic constellation of symptoms including necrolytic migratory erythema of the skin, weight loss, non-insulin-dependent diabetes mellitus, anemia, cheliosis, stomatitis, and an increased thrombotic tendency. Since pancreatic glucagonomas are predominantly located in the tail and findings of radiographic or sonographic examination can remain unspecific, patients often present already metastasis when diagnosis is first established, and can be difficult to differentiate from the other pancreatic tumors. We report the case of a 59-year-old woman with an malignant glucagonoma of the pancreas infiltrating already the spleen and presenting metastatic lesion in perirenal lymph nodes, and that the tumor was not assocated with the characteristic skin rash. The pateint with a past history of a diabetes mellitus and hypertension for 9 years was admitted with cramp-like left lower abdominal pain, watery diarrhea, and nausea. A solid tumor of tail of the pancreas revealed by ultrasonography and abdominal computed tomography and distal pancreatectomy, radical nephrectomy, and splenectomy were performed. Immunohistochemial examination of the tumor did show glucagon-reactive tissue and electron microscopy revealed many secretory granules, 180 to 300 nm in diameter in granulated cells. After pancreatic tumor resection, the patient had normalization of plasma glucagon and blood sugar. (Korean J Endocrine Surg 2002;2:120-123)

갑상선 수술의 해부학적 지표로서의 Zuckerkandl 결절의 중요성

윤지섭,정종주<SUP>1<,SUP>,이용상<SUP>1<,SUP>,남기현<SUP>1<,SUP>,정웅윤<SUP>1<,SUP>,장항석<SUP>1<,SUP>,박정수<SUP>1<,SUP>,Ji-Sup Yun,Jong Ju Jeong,<SUP>1<,SUP>,Yong Sang Lee,<SUP>1<,SUP>,Kee Hyun Nam,<SUP>1<,SUP>,Woong You 대한갑상선-내분비외과학회 2007 The Koreran journal of Endocrine Surgery Vol.7 No.4

Purpose: Zuckerkandl's tubercle (ZT) of the thyroid gland is a well-documented anatomical structure. This study evaluated the anatomical relationship of the ZT in terms of the recurrent laryngeal nerve (RLN) and the superior parathyroid gland (SP). Methods: The study included 325 patients (ten patients with benign tumors and 315 patients with malignancies) who underwent thyroid surgery between February and June 2007. Tubercles were classified according to size: Grade 0 (unrecognizable), Grade I (≤ 5 mm), Grade II (6∼10 mm) and Grade III (>10 mm). The incidence and size of the ZT and its positional relationship to the RLN and SP were investigated during thyroid surgery. Results: ZTs were identified in most patients (right thyroid 89.3%, left thyroid 85.6%). The percentageof tubercles according to grade and location was as follows: Grade 0, right thyroid 10.7% and left thyroid 14.4%; Grade I, right thyroid 7.9% and left thyroid 11.1%; Grade II, right thyroid 43.5% and left thyroid 38.5%; Grade III, right thyroid 37.9% and left thyroid 35.9%. The most common RLN course was in a groove between the ZT and the main body of the thyroid. Most of the SPs are situated cranial to the ZTs and were located at the 1 or 2 o'clock position (96.1%) in the left thyroid and at the 10 or 11 o'clock position (95.2%) in the right thyroid. A greater distance between the ZT and the SP was seen with a decreasing size of the ZT. Conclusion: The ZT was identified during most thyroidectomies, and there was a constant relationship between the ZT and either the RLN or SP. Therefore, identification of the ZT and an understanding of the relationship between the ZT and either the RLN or SP are essential for the performance of safe thyroid surgery. (Korean J Endocrine Surg 2007;7:237-241)

Effect of Surrogate Aggregates on the Thermal Conductivity of Concrete at Ambient and Elevated Temperatures

Yun, Tae Sup,Jeong, Yeon Jong,Youm, Kwang-Soo Scientific World, Inc 2014 The Scientific World Journal Vol.2014 No.-

<P> The accurate assessment of the thermal conductivity of concretes is an important part of building design in terms of thermal efficiency and thermal performance of materials at various temperatures. We present an experimental assessment of the thermal conductivity of five thermally insulated concrete specimens made using lightweight aggregates and glass bubbles in place of normal aggregates. Four different measurement methods are used to assess the reliability of the thermal data and to evaluate the effects of the various sensor types. The concrete specimens are also assessed at every 100°C during heating to ~800°C. Normal concrete is shown to have a thermal conductivity of ~2.25Wm<SUP>-1</SUP> K<SUP>-1</SUP>. The surrogate aggregates effectively reduce the conductivity to ~1.25Wm<SUP>-1</SUP> K<SUP>-1</SUP> at room temperature. The aggregate size is shown not to affect thermal conduction: fine and coarse aggregates each lead to similar results. Surface contact methods of assessment tend to underestimate thermal conductivity, presumably owing to high thermal resistance between the transducers and the specimens. Thermogravimetric analysis shows that the stages of mass loss of the cement paste correspond to the evolution of thermal conductivity upon heating. </P>

Synergistic Integration of Mesenchymal Stem Cells and Hydrostatic Pressure in the Expansion and Maintenance of Human Hematopoietic/Progenitor Cells

Kang, Yun Gyeong,Jeong, Jee-Yeong,Lee, Tae-Hee,Lee, Ho Sup,Shin, Jung-Woog Hindawi 2018 Stem cells international Vol.2018 No.-

<P>Ex vivo expansion of hematopoietic stem/progenitor cell (HSPC) has been investigated to improve the clinical outcome of HSPC transplantation. However, ex vivo expansion of HSPCs still faces a major obstacle in that HPSCs tend to differentiate when proliferating. Here, we cocultured HSPCs with mesenchymal stem cells (MSCs) and divided the HSPCs into two fractions according to whether they came into adherent to MSCs or not. Additionally, we used hydrostatic pressure (HP) to mimic the physical conditions <I>in vivo</I>. Even nonadherent cells expanded to yield a significantly larger number of total nucleated cells (TNCs), adherent cells maintained the HSPC phenotype (CD34<SUP>+</SUP>, CD34<SUP>+</SUP>CD38<SUP>−</SUP>, and CD133<SUP>+</SUP>CD38<SUP>−</SUP>) to a greater extent than nonadherent cells and had superior clonogenic potential. Moreover, applying HP significantly increased the number of TNCs, the frequency of the immature HSPC phenotype, and the clonogenic potential. Furthermore, the genetic markers for the HSPC niche were significantly increased under HP. Our data suggest that the nonadherent fraction is the predominant site of HSPC expansion, whereas the adherent fraction seems to mimic the HSPC niche for immature cells. Moreover, HP has a synergistic effect on expansion and functional maintenance. This first study utilizing HP has a potential of designing clinically applicable expansion systems.</P>

근접장 광 저장 장치를 위한 렌즈 조립

신윤섭 ( Yun Sup Shin ),박진무 ( Jin Moo Park ),이정욱 ( Jeong Uk Lee ),정미현 ( Mi Hyun Jeong ),서정교 ( Jeong Kyo Seo ),최인호 ( In Ho Choi ) 정보저장시스템학회 2005 추계학술대회논문집 Vol.2005 No.-

Air gap control method using a conventional actuator for near field recording technology has been reported. Toachieve good performance in the air gap servo and further experiments, the assembling between OL and SIL is the critical procedure. A lens set with NA 1.45 at 405 nm has been assembled and tested by using a Twyman-Green interferometer. The tolerance of assembling is very tight and the designing and adjusting adjusting procedure must be carefully controlled.

A study on sterilization of ballast seawater by the electrolytic-reduction treatment system

Yong-Sup Yun,Bok-Soo Kim,Jeong-Heum Yun,Jun-Yong Park 한국표면공학회 2013 한국표면공학회 학술발표회 초록집 Vol.2013 No.11

생식기능 장애 환자에서의 세포유전학적 이상

윤정섭 ( Jeong Sup Yun ),장성규 ( Sung Kyoo Jang ),최욱환 ( Ook Hwan Choi ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.5

목적: 본 연구는 염색체 이상과 남성과 여성의 생식능 장애와의 연관성을 알아보고 9번 염색체의 역위의 표현형과의 연관성을 확인하고자 시행하였다. 방법: 1995년 1월부터 2003년 12월까지 본원 세포유전 검사실에 의뢰되어 검사를 시행한 1713명의 염색체 분석 결과를 분석하였다. 연구 대상은 불임인 658명의 남성과 18명의 여성군, 반복 자연유산을 경험한 65명의 남성과 109명의 여성군, 일차성 무월경인 78명의 여성군, 61명의 이차성 무월경군으로 생식능 장애가 없는 남성 382명과 여성 342명을 대조군으로 정하였다. 대상군에서의 9번 염색체 역위의 빈도를 대조군과 통계학적으로 비교하였다. 결과: 염색체 이상은 남성 불임군에서 110예 (16.7%), 여성불임군에서 2예 (11.1%), 반복자연유산군의 남성과 여성에서 각각 3예 (4.6%), 15예 (13.8%), 일차성 무월경군에서 29예 (37.2%), 그리고 이차성 무월경군에서 10예 (16.4%)로 나타났다. 여성불임 환자와 이차성 무월경 환자에서는 생식능 장애와 염색체 이상 소견과의 관련성은 없었다. 반복자연유산을 하는 여성에서 6예 (5.5%)는 상염색체 전위, 그리고 7예 (6.4%)에서는 9번 염색체의 역위를 보였다. 일차성 무월경 환자에서는 다른 연구들에서와 같이 대부분의 염색체 이상은 터너 증후군을 포함한 성염색체의 이상이었다. 총 1713명의 염색체 분석에서 33예의 9번 염색체 전위가 발견되었다 (1.93%). 남성 불임의 경우, 10예 (1.52%)에서 9번 염색체의 전위가 발견되었고, 이는 남성 대조군과 큰 차이를 보이지 않았으나, 반복자연유산을 하는 여성군에서의 7예 (6.42%)의 9번 염색체 역위는 여성 대조군에 비해 유의하게 높았다 (p<0.05). 결론: 생식능 장애가 있는 많은 환자들은 다양한 세포유전학적 이상을 가지고 있기 때문에, 염색체 분석은 불임, 반복자연유산과 무월경을 평가하는 검사로 고려되어야 할 것으로 생각된다. 그리고 9번 염색체의 역위가 여성의 반복자연유산에서 많은 빈도를 보인다는 것을 알 수 있었다. Objective: The aim of this study was to investigate the relationship between chromosome abnormalities and male or female reproductive dysfunction and to be convinced of the role of pericentric inversion of chromosome 9 (inv (9)) on human phenotypes. Methods: Between Jan. 1995 and Dec. 2003, results of 1713 chromosomal analyses which were referred to our cytogenetic laboratory were analyzed. Study groups consisted of 658 cases of men and 18 cases of women with unexplained infertility, 65 cases of men and 109 cases of women with history of recurrent spontaneous abortion, 78 cases of women with primary amenorrhea, 61 cases of women with secondary amenorrhea and, 382 cases of men and 342 cases of women with no reproductive dysfunction (control group). The incidence of inv (9) among each group was compared with control group. Results: Chromosomal abnormalities were found in 110 cases (16.7%), 2 cases (11.1%), 3 cases (4.6%), 15 cases (13.8%), 29 cases (37.2%) and 10 cases (16.4%) in each group. The incidence of chromosomal abnormalities in male infertility was higher than previous reports, and 10 cases of inv (9) were detected in male infertility group. In cases of women with infertility and secondary amenorrhea, we couldn`t find the relevance between reproductive dysfunction and chromosomal abnormality. In cases of women with recurrent spontaneous abortion, 6 cases (5.5%) had autosomal translocation and 7 cases (6.42%) had inv (9). In cases with primary amenorrhea, most chromosomal abnormalities found were related to sex chromosome such as Turner`s syndrome, similar to other investigations. Thirty three cases of inv (9) was detected among the whole 1713 chromosomal analyses (1.93%). In cases of male infertility, 10 cases (1.52%) had inv (9), not significantly different with male control group. But 7 cases (6.42%) of inv (9) in women with recurrent spontaneous abortion were significantly higher than female control group (p<0.05). Conclusion: Because considerable proportion of patients with reproductive dysfunction had various cytogenetic abnormalities, the chromosomal analysis should be considered as a diagnostic tool in the evaluation of reproductive dysfunction such as infertility, recurrent spontaneous abortion, and amenorrhea. We also found that Inv (9) had a significantly increased incidence in female recurrent spontaneous abortion.

잠재적 생균제로서 식물 젖산균의 분리 및 특성

김정도 ( Jeong Do Kim ),박성보 ( Sung Bo Park ),이나리 ( Na Ri Lee ),정진하 ( Jin Ha Jeong ),이희섭 ( Hee Seob Lee ),황대연 ( Dae Youn Hwang ),이종섭 ( Jong Sup Lee ),정성윤 ( Seong Yun Jeong ),손홍주 ( Hong Joo Son ) 한국미생물생명공학회(구 한국산업미생물학회) 2011 한국미생물·생명공학회지 Vol.39 No.3

Plant lactic acid bacteria were isolated from plant-associated fermentative foods and crops, and their probiotic properties were investigated. Isolates K27 and O2 were isolated from Kimchi and onion, and identified as Lactobacillus plantarum on the basis of 16S rRNA gene analysis. The two strains were highly resistant to acid (an MRS broth at pH 2.5), where the survival rates of L. plantarum K27 and L. plantarum O2 were 90.2% and 97.3%, respectively. L. plantarum K27 and L. plantarum O2 also showed high bile resistance to 0.5% oxgall, with a more than 70% survival rate. They showed an inhibitory effect against pathogenic strains of Escherichia coli KCCM 40880 and Pseudomonas aeruginosa ATCC 10145. The antibacterial effect of the two strains was probably due to the presence of lactic acid. ACE inhibitory activities of the two strains ranged from 72.8% to 80.6% in MRS broth. Notably, the two strains showed high ACE inhibitory activity (89.2~98.2%) in MRS broth containing 10% skim milk. Antioxidant activity was tested by DPPH radical scavenging activity, with antioxidant activities of the strains being in the range of 56.8~61.5%. The results obtained in this study suggest that L. plantarum K27 and L. plantarum O2 may be potential probiotic starter cultures with applications with fermentative products.