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Jeong Sheop Shin(申正燮),Seung Jae Lee(李承再),Kuen Woo Park(朴權瑀) 한국육종학회 1995 한국육종학회지 Vol.27 No.1
A total of 39 genotypes of watermelon were chosen to identify the frequency of RAPD polymorphisms and to group into sub-populations. Of the 15 primers tested, 14 primers except primer 275 showed the polymorphic pattern. A total of 162 bands were generated across all 39 genotypes. Among them, 100(62%) were polymorphic and the remaining 62(38%) were monomorphic. The mean 1-F(genetic distance) was 0.114, and this value corresponded to the mean value of 0.117 detected in 36 genotypes of sorghum. The hightest value was 0.309 and the smallest one was 0.012. The 100 polymorphic fragments of total 162 bands were proved to be very reliable and confident, and selected as useful polymorphic markers. The mean value in this comparison was 0.24 and the highest onewas 0.69. Eight RAPD markers were expected to be utilized in the unique variety discrimination of eight watermelon genotypes. Using DNA polymorphisms, the two major clusters were resolved and one of these was subdivided into three cluster. Each sub-group were characterized and identified again with morphological and genetic characteristics of respective genotype.
Nonsense-mediated mRNA decay factors, UPF1 and UPF3, contribute to plant defense.
Jeong, Hee-Jeong,Kim, Young Jin,Kim, Sang Hyon,Kim, Yoon-Ha,Lee, In-Jung,Kim, Yoon Ki,Shin, Jeong Sheop Japanese Society of Plant Physiologists 2011 Plant & cell physiology Vol.52 No.12
<P>In Arabidopsis, the NMD-defective mutants upf1-5 and upf3-1 are characterized by dwarfism, curly leaves and late flowering. These phenotypes are similar to those of mutants showing constitutive pathogenesis-related (PR) gene expression, salicylic acid (SA) accumulation and, subsequently, resistance to pathogens. The disease symptoms of upf1-5 and upf3-1 mutants were observed following infection with the virulent pathogen Pst DC3000 with the aim of determining whether the loss of nonsense-mediated mRNA decay (NMD) is involved in disease resistance. These mutant plants showed not only enhanced resistance to Pst DC3000, but also elevated levels of endogenous SA, PR gene transcripts and WRKY transcripts. UPF1 and UPF3 expression was down-regulated in Pst DC3000-infected Arabidopsis plants, but the expression of various NMD target genes was up-regulated. The expression of 10 defense-related genes was elevated in cycloheximide (CHX)-treated plants. The transcriptional ratios of eight of these 10 defense-related genes in CHX-treated to non-treated plants were lower in NMD-defective mutants than in the wild-type plants. These eight defense-related genes are possibly regulated by the NMD mechanism, and it is clear that an alternatively spliced transcript of WRKY62, which contains a premature termination codon, was regulated by this mechanism. Taken together, our results suggest that UPF1 and UPF3, which are key NMD factors, may act as defense-related regulators associated with plant immunity.</P>
Jeong Sheop Shin(申正燮) 한국육종학회 1992 한국육종학회지 Vol.24 No.3
Genomic single or low copy number DNA clones from random genomic DNA libraries using the plasmid vector pBR322 and the phage λgtll were constructed using DNA from a barley cultivar, Sacheon #6. This work was done to develop the barley RFLP DNA markers and to saturate the linkage groups. In the initial screening, 25 clones, which were expected to have single or low-copy sequence, were selected from 1,200 bacterial colonies and 50 genetically unique phage plaques. Of these, 10 clones showed polymorphisms. To do these works, DNAs that were isolated from the barley lines and cultivars, MMS, Apex, Olbori, Sacheon #6, were digested with 3 restriction enzymes, Bam HI, Hind Ⅲ and Eco RI to prepare the genomic blot. Chemiluminescence as well as nick-translation was utilized to label the probe.
Genetic analyses on rice blast resistant alleles derived from mutant rice lines of Namil
Ji-Ung Jeung,Kyung-Ho Kang,Young-Seop Shin,Jeong-Sheop Shin 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Narrow genetic diversity of Korean commercial rice lines have been a major limit factor in breeding new breeding lines having resistance and tolerance against biotic and abiotic stresses. Introducing novel favorable allele types could be possible through crossing with wild relatives, it demands additional tedious efforts to restore the unique genetic background of the recurrent parents, which determine commercial value in the market. Our study is preliminary based on the mutation breeding, by which agronomic traits could be acquired with the least impact on the unique haplotype of the wild type. Through screening and evaluating more than 7,000 mutant lines of Namil, a high yielding Korean japonica cultivar, several dozens of mutant lines expressing improved performances in terms of resistance or tolerance against biotic or abiotic stresses. One Sodium azide treated mutant line, Namil(SA)M2-1063-11-1-1-1-1-1-1, designated as ‘Namil(SA)-bl5’ performed high level of resistance against rice blast as well as reduced culm length. Two mapping populations, to dissect genetic basis of the blast resistance and short culm length, were constructed by using F2 progenies derives crosses between Namil(SA)-bl5 and Milyang23 and Namil(wild type) and Milyang23. Each progenies were evaluated in terms of DNA marker genotype as well as basal agronomic traits including blast resistance by using F2:3 seeds. Association analysis between marker genotype and evaluated phenotype of progeny lines were adopted to localize the putative chromosomal locations involved to culm length and blast resistance. The putative locations unique to Namil(SA)-bl5 were then elucidated through the comparisons with those of Namil x Milyang23 reference population. Tentatively, the genetic factors for reduced culm length and blast resistance were identified on chromosome 7 and chromosome 12, respectively.