느타리버섯 신품종 육성 연구 : 저온성 느타리버섯 신품종 '설안'

지정현 외 한국버섯학회 2006 한국버섯학회지 Vol.4 No.3

느타리버섯의 품종 다양화를 위헤 단핵균주교잠에 의해 육성된 설안느타리버섯의 주요특성은 다음과 같다. 가. 균사생장적온은 26~30℃이고 버섯발생 및 생육온도는 10~15℃ 이다. 나. 갓색은 회색이고 얕은 깔대기형이며 발이수가 많다. 다. 병재배시 배양일수는 20℃에서 25일, 초발이 소요일수는 6일이며 대형태는 굵고 짧은형으로 병당수량은 138.8g 이었다. 라. 봉지재배시 배양일수는 20℃에서 23일, 초발이 소요일수는 5일이며 대형태는 굵고 짧은형이었고 봉지당 수량은 250.9g을 나타냈다. 마. 버섯의 균일성에 있어서 갓색과 갓형태의 이형개체 발생은 없었고, 균사생장량 및 종균배양기간에서도 지역 간 균일한 결과를 나타내었다. 바. 세균성 갈변병과 푸른곰팡이병에 대해서는 저항성이 약하나 재배 환경관리가 적정 할 경우 병해의 발생은 없었다. 사. DNA다형성을 비교 분석 한 결과 OPC8, URP7, URP8 등의 모든 primer에서 대조구인 원형느타리 1호와는 완전히 다른 밴드양상을 나타내어 품종간의 구분이 확실하였고, URP7 primer에서는 교잡계통과도 다른 밴드양상을 나타내었다. 아. 균사는 활력이 강하고 병, 봉지재배 적응성이 높고 발이량이 많은 평이다. 자. 생육중의 환기요구량은 적은 편으로 환기량이 많을 경우 대가 짧고 갓이 조기 개산하므로 품질이 떨어질 우려가 있다. 'Seolan', a new variety of oyster mushroom was developed by Mushroom Research Institute, Gyonggi Province Agricultural Research and Extension Services in 2005. It was bred with mating between monokaryotic strains isolated from KME35124 and F·X3. The major characteristics of the mushroom was shown a lot of primordia, the gray-colored and bunch pileus. The optimum temperature for the mycelial growth was around 26~30℃ and that for the primordia and growth of fruit-body was around 10~15℃. Incubation period was required amund 25 days with bottle culture and about 23 days in Poly Prophylene(P.P) plastic bag culture. The yield was shown high by 138.8g/bottle and 250.9g/P.P bag.

국가정책체제변화의 이론과 실제 : 경제개발 정책체제에서 사회개발 정책체제로 from the Economic-Developmental Policy Regime to the Social-Developmental Policy Regime

丁相賢,安致順 又石大學校 1992 論文集 Vol.14 No.-

큰양송이 Portobella 균주의 배양조건 및 형태적 특성

지정현 외 한국버섯학회 2003 한국버섯학회지 Vol.1 No.1

국내외에서 수집된 큰양송이(포토벨라)의 균주와 양송이 ASI707을 공시하여 균사체 배양조건과 균주별 자실체의 형태적 특성을 조사한 결과는 다음과 같다. 퇴비추출배지에서 균사생장이 우수하였고, 최적배양온도는 25℃, pH는 7.0 이었다. 균사생장에 가장 적합한 탄소원으로 Sucrose와 Starch, 질소원으로는 Malt extract가 선발되었고, 최적 C/N율은 10이었다. 인공재배한 자실체는 공시균주간에 갓크기, 갓두께 등에서 큰 차이가 없었으나 개체중은 25.8~33.8g 내외로 균주간에 다소 차이를 보였다. 공시균주 중 KME59007은 다발성으며 평당 수량도 44.6kg으로 가장 많았다. The compost dextrose(CD) medium was selected as an optimum liquid culture media for Portobella(Agaricus brunescens). The optimal conditions for the mycelial growth of Agaricus brunescens were 25 and pH 7.0. Sucrose and soluble starch were favorable to mycelial growth. Malt extract and ammonium nitrate appeared to be more favorable than the other kinds of nitrogen sources. The optimum C/N ratio was about 10.When the collected Portobella strains were compared with growth characteristics, KME 59007 appeared the highest of 44.6kg/3.3m².

항혈청 투여에 따른 돼지 전염성 위장염 예방효과 : Ⅰ. 혈청학적 결과, RT-PCR 검사, 형광항체검사 Ⅰ. Serological Results, Rt-PCR for Fecal and Small Intestin, FA Test

지영철,한정희,권혁무,한태욱,정현규,박봉균 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against transmissible gastroenteritis virus (TGEV) infection in piglets by administration of the TGEV antiserum orally at 5 hrs, 24 hrs and 36 hrs after birth. five piglets adiminstered the antiserum were experimentally infected with TGEV at four-day-old. Control group were four piglets infected with TGEV only. Serum antibody titers against TGEV were examined by serum neutralization (SN) test, dectection for TGEV or TGEV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the TGEV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of TGEV in feces and small intestines by RT-PCR were 24.5% and 20.0% in TGEV antiserum treated group and 44.0% and 75.0% in control group, respectively. 3. The detection rate of TGEV antigen in the small intestine by IFA were 26.7% in TGEV antiserum treated group and 75.0% in control group, respectively. It was concluded that oral administration of antiserum against TGEV to piglets was effective in preventing TGEV infection.

Identifying the more suitable nostril for nasotracheal intubation using radiographs

Chi, Seong In,Park, Sookyung,Joo, Li-Ah,Shin, Teo Jeon,Kim, Hyun Jeong,Seo, Kwang-Suk The Korean Dental Society of Anesthsiology 2016 Journal of Dental Anesthesia and Pain Medicine Vol.16 No.2

Background: One nostril must be selected for nasotracheal intubation. In some cases, structural anomalies within the nasal cavity hinder the insertion of the tube or complications, such as epistaxis, develop. This study examined the possibility of using radiography to select the nostril that would induce fewer complications. Methods: Four hundred and five patients who underwent nasotracheal intubation under general anesthesia were studied. A 7.0-mm internal diameter nasal right angle endotracheal (RAE) tube and 6.5-mm internal diameter nasal RAE tube were inserted into men and women, respectively. Complications were considered to have developed in cases in which insertion of the tube into the nasal cavity failed or epistaxis occurred. The tube was inserted into the other nostril for insertion failures and hemostasis was performed in cases of epistaxis. The degree of nasal septal deviation was determined from posteroanterior skull radiographs or panoramic radiographs; the incidence of complications was compared depending on the direction of the septal deviation and the intubated nostril. Results: The radiographs of 390 patients were readable; 94 had nasal septum deviation. The incidence of complications for cases without nasal septum deviation was 16.9%, that for cases in which the tube was inserted into the nostril on the opposite side of the deviation was 18.5%, and that for cases in which the tube was inserted into the nostril with the deviation was 35.0%, showing a high incidence of complications when intubation is performed through the nostril with septum deviation (chi-square test, P < 0.05 ). Conclusions: Although there were no differences in the incidence rates of complications between intubation through the left nostril and that through the right nostril, radiological findings indicated that incidence of complications significantly increased when the tube was inserted into the nostril with the septum deviation.

항혈청 투여에 따른 돼지 유행성 설사병 예방효과 : Ⅱ.임상증상, 병리조직학적 검사, 면역조직학적 검사 Ⅱ.Clinical sign, Histopathological Lesion and Immunohistochemical Finding

지영철,한정희,권혁무,정현규,이함희 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against porcine epidemic diarrhea virus (PEDV) infection in piglets by administration of the PEDV antiserum orally at 2 hrs, 24 hrs and 36 hrs after birth. six piglets adiminstered the antiserum were experimentally infected with PEDV at five-day-old. Control group were four piglets infected with PEDV only. Clinical signs and gross, histopathological lesion and immunohistochemical findings were examined. The results obtained were as follows; 1. In clinical signs, piglets of the control group appeared the typical signs of severe watery diarrhea, depression and anorexia but piglets of the PEDV antiserum treated group recovered progressively. In mortality, control group showd 75%, but PEDV antiserum treated group showed 16.7%, respectively. 2. In gross findings, piglets of the control group appeared the typical findings of congestion, distension of lumen, contaning curdes of undigested milk in stomach. But piglets of the PEDV antiserum treated group appeared milder than those of control group. 3. In histopathological findings, piglets of the control group appeared the typical findings of villous atrophy and fusion, congesion, exfoliation, vacuolation, squamation, loss of cilia and proliferation of crypt. But piglets of the PEDV antiserum treated group appeared milder than those of control group. 4. In immunohistochemical findings, piglets of the PEDV antiserum treated group showed more intensive in reaction for IgG and IgG than those of control group. The recation for IgA was stronger than that of IgG. It was concluded that oral administration of PEDV antiserum to piglets was effective in preventing PEDV infection and reduced their mortality.

항혈청 투여에 따른 돼지 전염성 설사병 예방효과 : Ⅰ.혈청학적 결과, RT-PCR 검사, 형광항체검사 Ⅱ.Serological Results, Rt-PCR for Fecal and Small Intestin, FA Test

지영철,한정희,권혁무,한태욱,정현규,박봉균 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against porcine epidemic diarrhea virus (REDV) infection in piglets by administration of the PEDV antiserum orally at 2 hrs, 24 hrs and 36 hrs after birth. six piglets adiminstered the antiserum were experimentally infected with PEDV at five-day-old. Control group were four piglets infected with PEDV only. Serum antibody titers aginst PEDV were examined by serum neutralization (SN) test, dectection for PEDV or PEDV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the PEDV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of PEDV in feces and small intestines by RT-PCR were 26.2% and 16.7% in PEDV antiserum treated group and 48.1% and 75.0% in control group, respectively. 3. The detection rate of PEDV antigen in the small intestine by IFA were 0% in PEDV antiserum treated group and 50.0% in control group, respectively. It was concluded that oral administration of antiserum against PEDV to piglets was effective in preventing PEDV infection.

느타리버섯 신품종 육성 연구(I) : 병재배용 신품종『진미』느타리버섯의 특성

지정현,주영철,최종인 한국버섯학회 2004 한국버섯학회지 Vol.2 No.4

병재배 느타리버섯의 품종 다양화를 위해 단핵균주교잡에 의해 육성된 진미느타리버섯의 균사생장적온은 25∼30℃, 버섯발생 및 생육온도는 15±1℃이고 갓색은 회색이며 얕은깔대기형으로 발이수가 많았다. 배양일수는 20℃에서 22일, 초발이 소요일수는 4일이며 가늘고 긴형으로 농가실증시험에서 병당수량은 145.9g으로 높은 편이었다. 버섯의 갓색과 갓형태, 이형개체 발생,, 균사생장량, 종균배양기간 등에 대한 균일성도 양호하였고, 세균성 갈변병에 대한 저항성은 소정도로 나타났으나 푸른곰팡이병은 발생되지 않았다. 또한 균사 활력이 강하고 15 이하에서는 진한회색을 나타내며 육질이 부드럽고 대가 백색으로 깨끗한 직립형의 특성을 보유하고 있다. Jinmi oyster mushroom(Pleurotus ostreatus) were bred in Mushroom Research Institute, Gyonggi Province A.R.E.S in 2003. This oyster mushroom were bred and cultivated one after mating single spores collected from K3-2 and ASI2018-249. The major characteristics of the mushroom are showing a lot of pinheadings, the gray-colored and infundibuliform pileus. The optimum temperature for the mycelial growth was around 25∼30℃ and that for the pinheading and growth of fruitbody was around 15±1℃. Around 22 days at 20℃ were required for incubation of Jinmi and the yield was shown high by 145.9g/bottle.

부산광역시 승용자동차 주행중 전신진동 및 소음도 평가

옥치상,모현주,정재열 高神大學校保健科學硏究所 1997 보건과학연구소보 Vol.7 No.-

There are many vibrations and noixes during vehicle use in urban although pavement road. Those are from engin vibarations, tire and wheel related vibrations. And the vibrations of vehicle use cause frequently las variation of road state. This study investivated the whole-body vibration and noise caused as variation of road state and speeds during automobile duiving in Pusan City, Korea Based on the results, exposure to whole body vibration and noise during vehicle use was higher at 70km/h than 50km/h in speed. On the floor of vehicle at speed 50km/h and 70km/h, the the mean values of energy equivalent acceleration and permissible exposure time by vebrational performance were 99.2cm/s²(1512.4 min), 200.3cm/s²(1939.5 min)in x axis ; 132.3cm/s²(1035.9 min),182.3cm/s²(1278.2 min) in y axis;87.1cm/s²(2774.6 min),234.8cm/s²(4196.4 min) in z axis, respectively. And on the seat of vehicle at speed 50km/h and 70km/hr,82.0cm/s²(597.1 min),63.2cm/s²(757.4 min), 157.2cm/s²(1286.8 min) in z axis, respectively. Permissible noise exposure time(PNET) caused during vehicle use were 80hr, 184hr in speed 50km/h and 57hr, 116hr in speed 70kn/h when doors were closed and open,respectively.

우관상동맥 개구부 완전 폐쇄가 동반된 대동맥 판상부 협착증 1례

임상현,김종진,김희열,진승원,전희경,노태호,채장성,김재형,홍순조,최규보,윤정섭,김치경 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4