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고정환 ; 윤옥현 ; 박홍기 ; 전기환 ; 김상민 ; 김학민 ; 박희룡 김천대학교 1999 김천대학교 논문집 Vol.20 No.-
The social education that is provided for the people will be an important part of the national growth in the 21 century. This study was taken to present the desirable direction that Kimcheon College Continuing Education has to make progress as a central organization with perceiving the importance of continuing education for the Kimcheon citizen. To achieve the objective of the study, examined a sample of 592 citizen and students from Kimcheon City and Kimcheon College. The survey revealed the following results : 1. Offer the effective operation of education policies and curriculums. 2. Perform the role of foreign language education center. 3. Introduce the saving credit system. 4. Operate the special lectures for the students of the high school graduating class after the national scholastic achievement test for university and college entrance. 5. Suppert the special skills education for high school students after-school hours. 6. Administer the new employment program. These results showed the desirable direction that the Kimcheon College Continuing Education has to take some actions for the Kimcheon citizen.
Achievements and vision of the global network for cancer biomarker development
Jeong-Heon Ko 한국당과학회 2011 한국당과학회 학술대회 Vol.2011 No.1
Our center, Daejeon-KRIBB-FHCRC Research Cooperation Center, was established to develop ‘cancer biomarkers’ in collaboration with Fred Hutchinson Cancer Research Center (FHCRC) in Feb. 2005 with supports from the Daejeon Metropolitan City. Cancer is often difficult to achieve early diagnosis, which is, however, a decisive requisite for favorable outcome in cancer treatments. In order to develop the cancer biomarkers, we’ve tried to pinpoint cancer cells-originating aberrant glycoproteins which would be a well-grounded approach to cancer biomarker discovery ultimately using blood. One of the glycosyltransferases responsible for aberrant glycosylation in cancer is N-acetylglucosaminyltransferase V (GnT-V), which catalyzes an addition of b1,6-N-acetylglucosamine (GlcNAc) to the core N-glycan, and many lines of evidence have demonstrated the role of N-acetylglucosaminyltransferase V (GnT-V) in cancer development. Tissue inhibitor of metalloproteinase-1 (TIMP-1) and protein tyrosine phosphatase kappa (PTPk) were good models involved in cancer malignancy upon aberrantly glycosylation. Basically by adopting multi-lectins enrichment strategy, candidate proteins showing high sensitivity and specificity during the discovery phase were selected and the panel of biomarker candidates is currently under in-depth analyses for validation. Validation is a time-consuming step for biomarker developments requiring confirmation of the biomarker candidates through multiple pairs of clinical samples. That is why a sensitive, multiplexing validation method is necessary. To address this challenge, we are developing as a DNA-tagged antibody-based ‘New’ validation method, enabling a multiplexed validation of biomarkers. Furthermore, the collaboration with the FHCRC leads to an expansion of the ‘epigenetic’ studies to ‘glycogenome’, requiring incessant collaborations. Recently, Daejeon Metropolitan City became a ‘sister city’ for Sapporo City in Japan. It’s noteworthy that these relationships prompt to establish a ‘north pacific network of cancer biomarker development’ among the KRIBB, FHCRC and Sapporo Medical University.
Development of cancer glyco-biomarkers and its applications
Ko, Jeong Heon 한국당과학회 2016 한국당과학회 학술대회 Vol.2016 No.07
Cancer is often difficult to achieve early diagnosis, which is, however, a decisive requisite for favorable outcome in cancer treatments. Cancer biomarkers have been sought for several purposes preferably in blood and pinpointing cancer cells-derived aberrant glycoproteins would be a well-grounded approach to cancer biomarker discovery. One of the glycosyltransferases responsible for aberrant glycosylation in cancer is N-acetylglucosaminyltransferase V (GnT-V), which catalyzes an addition of b1,6-N-acetylglucosamine (GlcNAc) to the core N-glycan, and many lines of evidence have demonstrated the role of N-acetylglucosaminyltransferase V (GnT-V) in cancer development. Tissue inhibitor of metalloproteinase-1 (TIMP-1) and protein tyrosine phosphatase kappa (PTPk) were suggested to be involved in cancer malignancy upon aberrantly glycosylation by GnT-V. In addition to GnT-V, several glycosyltransferase co-works to render the altered glycan structures in cancer cells including sialyl Lewis antigen and core-fucosylation, which prompted us to mine serological biomarker candidates in cancer sera. Immunodepleted on an immune-LC column, serological proteins were enriched by carbohydrate beads conjugated with lectins like L-PHA, DSA, E-selectin, AAL, Con-A. The fraction refractive to lectin enrichments was resolved on an SDS-PAGE gel, and fractionated by molecular mass. Both the captured glycoproteins and gel-separated proteins were tryptic-digested for sequence determination in an LTQ-FTICR mass spectrometer. Candidate proteins showing high sensitivity and specificity during the discovery phase were selected and the panel of biomarker candidates is currently under in-depth analyses for validation.
Jeong, Hyun-Ja,Lee, Ju-Young,Kim, Joon-Bae,Go, Hoyeon,Ko, Seong-Gyu,Seo, Young-Wan,Jeong, Sejin,Park, Jinhan,Na, Ho-Jeong,Um, Jae-Young,Kim, Hyung-Min,Hong, Seung-Heon Gordon and Breach 2008 INTERNATIONAL JOURNAL OF NEUROSCIENCE - Vol.118 No.10
<P>KI0477959 (Herbkines) has been used for the purpose of development of physical strength in wasting diseases, like cancer. In the present study, apoptosis-inducing activities of butanol fraction of KI0477959 were studied in human leukemia cell line, HL-60 cells. KI0477959 increased cytotoxicity but had less effect on human peripheral blood mononuclear cells. KI0477959-induced apoptosis was accompanied by activation of caspase-3 and specific proteolytic cleavage of poly-ADP-ribose polymerase. Increased apoptosis was reduced by treatment with p38 and extracellular signal-regulated protein kinase (ERK) inhibitors. These results suggest that KI0477959 induces apoptosis through activation of caspase-3, p38, and ERK in HL-60 cells.</P>
Glyco-biomarkers and glycan humanized model mouse
Ko, Jeong Heon 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.07
Glycosylation ts one of the most frequent post-translational modification, playing an essential role m the normal development and physiology of cells. Altered expression of glycans and glycoconjugates has been associated with numerous pathologies, including congenital disorders and cancer. To achieve early diagnosis, cancer biomarkers have been sought for several purposes preferably in blood and pinpointing cancer cells-derived aberrant glycoproteins would be a well-grounded approach to cancer biomarker discovery. One of the glycosyltransferases responsible for aberrant glycosylation m cancer lS N-acetylglucosaminyltransferase v (GnT-V), which catalyzes an addition of betal ,6-N-acetylglucosamine (GicNAc) to the core N-glycan, and many lines of evidence have demonstrated the role of N-acetylglucosaminyltransferase V (GnT-V) in cancer development. Tissue inhibitor of metalloproteinase-1 (TIMP-1) and protein tyrosine phosphatase kappa (PTPk) were suggested to be involved in cancer malignancy upon aberrantly glycosylation by GnT-V. Although cancer biomarker candidates were drived with many lectins and mass spectrometry, an efficient validation method based on a sensitive and multiplexed platform was hampered. Although ELISA-based analytical methods are very powerful for detecting specific proteins, the lectin/antibody sandwich method, which simultaneously recognizes proteins and glycans, is difficult to apply because the antibodies themselves are glycoproteins. To overcome the associated hurdles in this study, antibodies were tagged with oligonucleotides with T7 promoter and then allowed to form a complex with corresponding antigens. An antibody-bound specific glycoform was isolated by lectin chromatography and quantitatively measured on a DNA microarray chip following production of fluorescent RNA by T7-trascription. This tool ensured measurement of targeted glycoforms of multiple biomarkers with high sensitivity and multiplexity, but there are difficulties in cost and reproducibility due to the many steps. Ultimately, in order to overcome this, we have made a mouse that produces a non-glycosylated antibody through a genome editing method, and a recent study on a glycan humanized mouse is also introduced -