Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

허혈심근에서의 퓨린성 및 콜린성 작동제의 심근보호 작용

김재하,박상덕,주정민,국현,조정관,박옥규 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.7

Platelet-Derived Growth Factor가 백서두개관 세포군의 증식 및 교원합성에 미치는 영향

김기수,고성희,백정화,민병무,김관식,정동균 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.2

To study the effect of platelet-derived growth factor(PDGF) on the replication and collagen synthesis of rat calvarial cells, five bone cell populations(I-V) were prepared from fetal rat calvaria by sequential enzyme digestion. After primary culture for 6-7 days, each bone cell population was collected and then population Ⅰ and Ⅱ, Ⅳ and Ⅴ were pooled together. And the cells were resuspended at 6-8×10^4 cells/㎝^2 and cultured for 2-3 days. The medium was changed to serum-free medium prior to addition of growth factor. The effect of PDGF on the cell proliferation was measured by the incorporation of [^3H]thymidine into DNA. Protein synthesis was determined by measurement of [^3H]proline incorporation into collagenase-digestible protein(CDP) and noncollagenous protein(NCP) according to the method of Peterkofsky and Die-gelmann(1971). The observed results were as follows. 1. PDGF at 10 ng/㎖ significantly increased the [^3H]thymidine incorporation into DNA in all bone cell populations. 2. PDGF at 30 ng/㎖ significantly increased the synthesis of NCP in population Ⅰ, Ⅱ and Ⅳ, Ⅴ. 3. PDGF had no effect on the synthesis of CDP but percent collagen synthesis was decreased significantly in population Ⅳ, Ⅴ. Taken together, the increase of protein synthesis by PDGF in rat calvarial cells was due to the incraese of NCP synthesis.

GIS 및 RS기법을 활용한 산사태 취약성 평가

김경태,정성관,박경훈,오정학 한국지리정보학회 2005 한국지리정보학회지 Vol.8 No.1

본 연구는 금호강 유역을 대상으로 GIS와 윈격탐사기법을 활용하여 산사태 취약성의 예측과 지도화를 수행하자 한다. 산사태 영향인자인 사면경사, 사면방4향, 지질, 토지이용도, 식생지수(NDVI)의 공간데이터베이스는 30m×30m 해상도로 구축하였다. 산사태 취약성은 중첩분석과 한산평가 매트릭스 방법으로 예측하였고, 6개 범주(안정, 매우 낮음, 낮음, 중간, 높음, 매우 높음)로 구분한 산사태 취약성 지도를 제작하였다. 분석결과에 따르면, 산사태 취약성이 '매우높은' 지역은 전체 대상지의 약 0.3% 정도를 차지하며, 이들 지역은 주로 높은 경사TA와 낮은 식생지수를 가지는 산림지역에 분포하는 것으로 나타났다. This study aims at predicting and mapping of the landslide susceptibility in the Geumho river watershed using GIS and Remote Sensing techniques. We constructed the spatial database of affecting factors such as slope angle, slope aspect, lithology, landuse, and vegetation index (NDVI) at a 30mx30m resolution. The landslide susceptibility of the study area was predicted through overlay analysis and adding up estimation matrix, and the predicted map of landslide susceptibility with six categories (stable, very low, low, moderate, high, very high) was constructed. As the results, it showed that the very high susceptibility zones made up approximately 0.3% of the total study area, and these zones were mainly distributed in the forest area with the high slope angle and low vegetation index.

구강점막섬유화에 의한 개구제한 : 증례보고 A CASE REPORT

김 훈,김수관,하정완 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

Oral submucous Fibrosis(OSF) is an insidious chronic disease affecting the mucosa of the oral cavity and occasionally extending into the pharynx and esophagus. It is associated with a genetic predisposition and alterations, infectious and viral agents, carcinogens, nutritional and immunological factors. the etiology is obscure but it is correlated with a hypersensitivity to capsaicin, secondary to chronic iron and/ or vitamin B complex deficiencies or exposure to cashew kernel oil. We report a patient who had a month opening limitation caused by a fall when he was 8 years old despite several treatments at other hospitals. Excellent results were obstained by releasing the scar tissue using a standard split-thickness skin graft(STSG) which obtained from the thigh. It is important to recognize as a precancerous condition and to inspect the excision site and the remaining oral mucosa during long-term follow-up on a regular basis.

온도가 파밤나방의 발욕에 비치는 영향

고현관,김정화 충북대학교 농업과학기술연구소 1991 農業科學硏究 Vol.9 No.1

Threshold temperature and effective day- degrees were calculated at constant temperature conditions. Egg periods were 5.4, 4.0, 3.0 and 2.0 days at 20℃, 23℃, 27℃ and 30℃, respectively. Larval periods were 36.8, 25.6, 15.8 and 14.5 days and pupal periods were 16.4, 11.2, 6.6 and 5.7 days at above temperature. Preovipositional periods were 2.3, 2.3, 1.3 and 0.8 days and female longevity were 9.0, 6.3, 5.3 and 4.5 days at 20℃, 23℃, 27℃ and 30℃, respectively. Relationships between constant temperature and the developmental velocity of egg, larva and pupa were linear. the calculated threshold temperatures of development were 14.4℃ in egg, 13.7℃ in larva, 15.1℃ in pupa and 14.2℃ in egg-pupa. Total effective day-degrees were 33.4 in egg, 229.5 in larva, 83.1 in pupa and 344.1 in egg- pupa.

Soft Agar Assay를 이용한 배양골조직과 골세포의 Transforming Growth Factor-β유리에 관한 연구

백정화,김관식,정동균 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.2

To study the effect of PTH, osteotropic hormone, on the TGF-β activity in conditioned medium prepared from bone explants, fetal rat ulnae and radii were removed at 19-day of gestation and organ cultured for 24 hours. Then media were changed with fresh BGJb media for control group or PTH-supplemented media (50 or 200 ng/㎖) for experimental group respectively and conditioned media were prepared by 2-day culture of explants. To study the cellular orgin of matrix-associatied TGF-β and the effect of PTH on the TGF-β activity in conditioned media prepared from bone cells, five bone cell populations were prepared from fetal rat calvaria by sequential enzyme digestion. After primary culture, each bone cell population was collected and resuspended as Ⅰ-Ⅱ, Ⅲ and Ⅳ-Ⅴ groups. After 24 hours, media were changed with fresh minimum essential medium(MEM) or 600 ng/㎖ PTH-supplemented media and conditioned media were prepared by 2-day culture. For activation of latent TGF-β activity in bone cell-conditioned media, media were acidified and then neutralized. TGF-β activities of conditioned media were measured by anchorage-independent growth of NRK fibroblasts using modified Todaro soft agar assay method(1987) and the number of colonies ≥ 50 ㎛ was counted. The observed results were as follows. 1. TGF-β, without 2 ng/㎖ EGF, did not induce colony formation in soft agar suspensions. 2. With 2 ng/㎖ EGF, TGF-β induced NRK cells to form large colonies in soft agar suspensions and colony numbers increased proportionally to TGF-β concentrations from 50 pg/㎖ to 1600 pg/㎖. 3. When ulnae and radii were incubated with 50 ng/㎖ PTH, TGF-β activity in conditioned media was not significantly different from control conditioned media. In contrast, TGF-β activity in conditioned media prepared from culture with supplementation of 200 ng/㎖ PTH, was significantly increased(p < 0.01). 4. TGF-β activity was detectable in all bone cell populations and the amount was not significantly different between cell populations. 5. When bone cell populations were incubated with 600 ng/㎖ PTH. TGF-β activity in conditioned media from each population was not significantly different from control conditioned media. These results suggest that all cell populations isolated from calvaria synthesize TGF-β and PTH has no apparent effects on the TGF-β production of bone cell populations. In addition, increased TGF-β activity in bone explant conditioned media by 200 ng/㎖ PTH appears to be due to the enhanced release of TGF-β from bone matrix secondary to bone resorptive process.

혈소판 풍부혈장의 임상적 응용

하정완,김수관,조세인,이철우,정태영,김수흥,김영균 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.5

Platelet-rich plasma is an autologous source of platelet-derived growth factor and transforming growth factor beta that is obtained by sequestering and concentrating platelets by gradient density centrifugation. We have used platelet-rich plasma for bone graft, especially allobone graft, at implant surgery, sinus lift procedure, and cyst enucleation. This article is retrospective study from October 1999 to November 2000. All cases were good healing and clinical success.

하악 무치악 인공치아매식술시 하치조신경 전위술

최의환,하정완,김수관,정태영,김수흥 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

Modified surgical technique for transposition of the inferior alveolar nerve followed by immediate placement of endosseous implants in mandibles with moderate to severe atrophy are presented. Five transpositions of the inferior alveolar nerve together with the installation of 10 implants were performed in four patients. The mean postoperative follow-up time was 17 months, with a range of 8 to 20 months. All implants with functioning pontics remained stable, with no mobility or symptoms of pain and infection during the follow-up period. Neurosensory evaluation was performed using the two-point discrimination test. Two patients had objective neurosensory dysfunction at postoperative, but all the nerve function were reported as normal by the patients 4 months postoperatively.