Curcumin protects retinal pigment epithelial cells against oxidative stress via induction of heme oxygenase-1 expression and reduction of reactive oxygen

Woo, Je Moon,Shin, Da-Yong,Lee, Sung Ju,Joe, Yeonsoo,Zheng, Min,Yim, Jin Ho,Callaway, Zak,Chung, Hun Taeg Molecular Vision 2012 Molecular vision Vol.18 No.-

<P><B>Purpose</B></P><P>To determine whether curcumin induces expression of the defensive enzyme heme oxygenase-1 (HO-1) and protects cells against oxidative stress in cultured human retinal pigment epithelial cells.</P><P><B>Methods</B></P><P>Effective concentrations and toxicities of curcumin were determined after 3 h of curcumin treatment with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Confluent human retinal pigment epithelium cell lines (ARPE-19) were preincubated with curcumin and oxidatively challenged with H<SUB>2</SUB>O<SUB>2</SUB>. HO-1 expression was determined with western blot analysis. To confirm the protective role of HO-1 in oxidative stress, small interfering RNA (siRNA) against HO-1 or inhibitor of HO-1 was treated with curcumin in retinal pigment epithelium cells. Intracellular levels of reactive oxygen species (ROS) were measured with flow cytometry and confocal microscopy. Apoptosis was evaluated with Annexin V-fluoroscein isothiocyanate staining.</P><P><B>Results</B></P><P>Curcumin had little cytotoxicity at concentrations less than 30 μM, and HO-1 expression was the highest at the 15 μM concentration. At this concentration, curcumin also increased the cytoprotective effect against the oxidative stress of H<SUB>2</SUB>O<SUB>2</SUB> through the reduction of ROS levels in human retinal pigment epithelial cells. Curcumin’s effect on the reduction of ROS was mediated by the increase in HO-1 expression.</P><P><B>Conclusions</B></P><P>Curcumin upregulated the oxidative stress defense enzyme HO-1 and may protect human retinal pigment epithelial cells against oxidative stress by reducing ROS levels.</P>

골수이식 후 골밀도 및 골대사의 변화 : 2년 추적관찰 2-Year Prospective Study

이원영,강무일,오은숙,오기원,한제호,손현식,윤건호,차봉연,이광우,손호영,강성구,신완식,민우성,김춘추 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

착화제와 유기산이 Wistar rat체내의 Sr 분포에 미치는 영향

이기호,이제호,박상윤,이승훈,유용운,윤택구 대한방사선 방어학회 1990 방사선방어학회지 Vol.15 No.2

Wistar rat에 85SrCl2를 꼬리 정맥에 주사하여 체내 기관과 혈액 내 분포, 잔존율을 조사하였고 착화제와 유기산을 투여하여 혈장 단백질에 결합하는 Sr양의 변화를 측정하였다. 혈액내에서 Sr은 혈장에 60%, 세포에 40%부착되어 이동하였다. 혈장에 존재하는 Sr중 약 50%정도는 혈장 단백질과 결합한 상태였고, 세표에는 세포 표면에 가볍게 부착되어 있었다. Erythrocyte나 granulocyte보다 lymphocyte에 많은 양의 Sr이 부착되어 있었다. 투여후 초기 1시간 이내에 혈액 내에서 급격히 감소하여 뼈에 침착되었다. 이때 각 기관에서도 Sr의 잔존율은 24시간 이내에 크게 감소하였고, 뼈로 침착된 Sr은 24시간 이후에 서서히 감소하였다. 착화제 EDTA, EGTA 및 DTPA를 투여한 경우, 혈장 단백질에 결합하는 Sr의 양은 대조군의 57%에서 27-33%로 감소하였으며 citrate 및 oxalate의 투여시는 이값이 19%와 40%로 각각 감소하였다. 85SrCl2 was injected to the tail vein of Wistar rats and investigated its distribution and clearance in the tissues and blood. We also measured the changes in Sr binding to the blood plasma protein by administrating chelating agents and organic acids. For the blood, 60% of the Sr occurred in the plasma and 40% on the cell membrane. Fifty percent of Sr in the blood plasma was bound to plasma protein. Sr on the cell membrane seemed to be bound loosely. The binding in the lymphocyte was higher than in the erythrocyte and granulocyte. Within one hour Sr was quickly disappeared from the blood stream, to be accumulated in the bone. Twenty four hours after the injection, Sr decreased rapidly in the organs of soft tissue, but slowly in the bone. The binding of Sr to plasma protien decreased from 57% of the control to 27-33% in the group treated with chelating agents. EDTA. EGTA and DTPA and to 19% and 40% in the groups treated with organic acids, citrate and oxalate, respectively.

조혈모세포이식술이 골수기질세포의 기원 및 조골세포로의 증식 및 분화에 미치는 효과

강무일,조성원,오은숙,백기현,이원영,오기원,김혜수,한제호,윤건호,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

Treacher Collins 증후군 환아의 증례보고

박지현,김승혜,송제선,김성오,이제호 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.3

Treacher Collins 증후군(TCS)은 25,000명에서 50,000명당 1명의 빈도로 비교적 드물게 발생하는 난치성 질환이다. 이 질환은 정상적인 부모에게서 돌연변이로 발생하거나 상염색체 우성으로 유전된다고 알려져 있다. 하악과 상악, 협골의 발육 부전, 부정교합, 양측성 이개 기형, 안검렬의 반몽고증 사면(antimongoloid slant)이 특징적이며, 종종 구순열, 구개열, 외이도 폐쇄, 중이와 내이의 기형에 의한 청각소실, 왜소증, 심장과 골격의 이상 등을 동반한다. 또한, 인두의 형성부전, 소구, 거설증, 악골의 기형에 기인하여 수면 중 무호흡증, 만성 폐쇄성 무호흡증 등의 호흡 장애가 있을 수 있으며, 전신마취 유도 시에 기관내 삽관이 어렵다고 보고된 바 있다. 치과의사는 관련 신드롬에 대한 충분한 이해를 통해 환자에게 보다 안전하게 치료를 제공해야 하며 구강 건강 관리 및 선천성 안면 기형에 대한 문제 해결을 돕기 위해 적절한 치료 및 안면 수술에 관련된 가이드를 제시해야 한다. 본 증례는 전반적인 치아우식을 주소로 연세대학교 치과병원 소아치과에 내원하여 외래에서 치아우식 치료를 받은 TCS 환아에 대한 것으로, 문헌 고찰을 통해 얻은 다소의 지견을 함께 보고하는 바이다. Treacher Collins syndrome(TCS) is a rare, incurable condition occurring in approximately 1 of 25,000 to 50,000 births. It may occur as a spontaneous mutation out of genetically normal parents or it may be manifested as an autosomal dominant pattern. TCS is characterized by facial deformities such as, underdevelopment of the maxilla, mandible and zygoma, malocclusion, bilateral deformities of auricles, antimongoloid slant of the palpebral fissures. The syndrome is often associated with cleft lip and palate, ear malformations and hearing loss, short stature, and anomalies of the heart and skeleton. Respiratory difficulty associated with air way obstruction may also be observed, and there is considerable difficulty in airway management during general anesthesia. It is necessary that dentists provide safe dental treatments and guidelines to TCS patients by providing adequate understanding about the characteristics of the syndrome and proper ways of managements. The purpose of this study is to report the dental and medical characteristics of the patient who visited the Department of Pediatric Dentistry, Yonsei University for multiple dental caries treatment and to review the literatures of TCS.

동종 골수이식을 시행받은 환자에서 폐색성 세기관지염에 의하여 발생한 자발성 기종격동과 피하 기종

이병환,이제중,이연경,안재숙,김여경,황호인,박무림,조상희,정익주,김형준 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

저자들은 만성골수성백혈병으로 동종 골수이식과 이식편 부전으로 인하여 추가적인 말초혈액 조혈모세포이식을 시행 받은 환자에서 만성 이식편대숙주질환과 그 폐 합병증인 폐색성 세기관지염에 동반된 자발성 기종격동과 피하 기종이 병발한 1예를 경험하였기에 이를 보고하는 바이다. Obstructive lung disorders following after allogeneic bone marrow transplantation (BMT) in association with graft- versus-host disease (GVHD) contribute significant morbidity and mortality. We report a case of a 28-year-old man who developed spontaneous pneumomediatinum and subcutaneous emphysema complicating bronchiolitis obliterans after allogeneic BMT. He received an allogeneic BMT for chronic phase of chronic myeloid leukemia. Five months after BMT, he was boostered by allogeneic peripheral blood stem cells from the same donor due to graft failure. One month after the boostering, chronic GVHD developed and were treated with cyclosporine and steroid. The patients developed spontaneous pneumomediatinum and subcutaneous emphysema secondary to severe bronchiolitis obliterans 4 months after boostering donor cells. The air-leak syndromes were recovered by conservative management, including high-flow oxygen.

다공질 실리콘 카바이드의 제작 및 광발광 성질

이기환,오제직,최석남,박병빈,이태호,김영유 공주대학교 사범대학 과학교육연구소 1998 과학교육연구 Vol.29 No.1

p-type α-SiC(4H, 6H, 15R polytype)를 사용하여 다공질 실리콘(porous silicon; PS)의 제작방법과 유사하게 HF/H₂O/C₂H??OH 용액에서 전기화학적으로 에칭을 하여 광발광 특성을 가지는 다공질 실리콘 카바이드(porous silicon carbide; PSC)를 제작하였다. PSC의 다공질의 분포는 전류밀도가 증가할수록 증가하고 다공질의 크기는 감소하였으며 광발광(photoluminescence; PL) 스펙트럼의 분석을 통해 전극 표면에서의 산화반응은 공급되는 전자의 양과 전류밀도에 의존하며 전류밀도가 증가할수록 전극의 산화반응은 억제된다는 결과를 얻었다. Porous silicon carbide(PSC) having photoluminescence(PL) can be prepared by a technique very similar to that used in porous silicon preparation, by electrochemical etching of crystalline p-type α-SiC(4H, 6H, 15R polytype) in ?? solution. The results show that porous distribution increase with increasing of the current densities while pore size decrease with that. Through the PL spectra analysis anodic oxidation of electrode surface was dependent on the amount of provided electrons and the current densities and inhibited with increasing of the current densities.

영광원자력발전소 주변의 식생과 식물현존량 추정

이호준,양효식,전영문,정흥락,강재구,방제용 建國大學校基礎科學硏究所 1996 理學論集 Vol.21 No.-

영광원자력발전소 주변의 식생에 대한 식물사회학적 조사를 실시하였으며, 순 1차 생산량과 식물현존량을 추정하였다. 또한 상관에 의해 현존식생도 및 녹지자연도를 작성하였다(1:50,000). Z-M 방식에 의한 삼림식생은 소나무군락과 곰솔군락으로 구분되었다. 전체적으로 교목층에 소나무와 곰솔, 아교목층에 졸참나무와 곰솔, 관목층에 털진달래와 신갈나무, 초본층에 털대사초와 그늘사초 등의 피복지수가 우세하게 나타났다. 토양의 pH는 4.65∼4.67, 유기물 함량은 7.28∼7.53%의 범위였다. 현존식생도에 의한 분포비율은 경작지(67%), 소나무(21.9%) 및 곰솔군락(9.1%)의 순으로 나타났으며, 녹지자연도는 등급 2가 67%(경작지), 7등급(이차림(A))과 6등급(조림지)이 각각 29.2%와 1.8%로 나타났다. Montreal model에의한 소나무림과 곰솔림의 식물현존량은 657,817.2와 177,367.0ton으로 각각 산정되었다. The forest vegetation and estimation of phytomass and net primary production in the vicinity of Yonggwang Atomic Power Plant were analyzed on the basis of the phytosociological data. Depending on physiognomy, the actual vegetation map and the degree of green naturality(DGN) were also drawn in the scale of 1:50,000. According to Z-M method, the forest vegetation was classified into two communities; Pinus densiflora and Pinus thunbergii communities. It was generally shown that Pinus densiflora and Pirus thunbergii in tree-1 layer, Quercus serrata and Pinus thunbergii in tree-2 layer, Rhododendron mucronulatum var. ciliatum and Quercus serrata in shrub layer, and Carex ciliato-marginata and Carex lanceolata in herb layer had high coverage index. The pHs and contents of organic matter of the forest soils collected in each sites were in the 4.64 to 4.67 and 7.28% to 7.53% in average, respectively. The distribution area of each vegetationindex. The pHs and contents of organic matter of the forest soils collected in each sites were in the 4.64 to 4.67 and 7.28% to 7.53% in average, respectively. The distribution area of each vegetation type in the actual vegetation map was 67% cultivated land, 21.9% in Pinus densiflora community and 9.1% in pinus thunbergii community. The distribution of the degree of green naturality(DGN) was 67% in the second-grade (cultivated land), 29.2% in 7th grade (second forest(A)) and 1.8% in 6th grade (plantation). It was estimated that phytomass of Pinus densiflora and Pinus thunbergii in the study sites, which was calculated by Montreal model, was 657,817.2 and 177,367.0 ton, respectively.

高X線 吸收유리에 있어서 BaO, SrO의 含量變化가 液相溫度에 미치는 影響

李卿喜,梁在翊,金德文,姜元浩 明知大學校 産業技術硏究所 1987 産業技術硏究所論文集 Vol.6 No.2

The liquidus temperature was observed according to the change of content BaO 6 W/O ~ 12W/O and SrO 1 W/O~4W/O in R₂O-PbO-Al₂O₃- (BaO+SrO+SiO₂) glass system. The liquidus temperature varied between 810°C ~ 950°C in these glasses. The effect of BaO & SrO content to the liquidus temperature of the glasses are as follows. 1. The variation of the liquidus temperature were remarkable by the content of SrO in the range of BaO contents from 6 W/O to 8 W/O. 2. The minimum liquidus temperature is located at the composition of BaO 8 W/O SrO 4 W/O SiO₂ 68 W/O.

고교 야구 선수들의 견관절 외회전 및 내회전근의 근력 및 그 특성

이제운,윤승호 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

The imbalance of rotator muscles of shoulder frequently causes shoulder injury in many baseball players. This study was intended to evaluate the strength characteristics of external and internal rotator muscles in high school baseball players. Isokinetic tests of external/internal rotators were performed using Cybex Ⅱ^+ Isokinetic dynamometer in nine pitchers, thirteen nonpitchers and twenty normal control subjects. And the flexibility of external rotator muscles of shoulder was evaluated by the hand-to-shoulder-blade Test. The results were as follows : 1) In pitchers, mean peak torque ratios of external/internal rotators were significantly high in nondominant side at 180 and 300 deg/sec, mean peak torque and mean peak torque body weight ratios of external rotators were significantly high in nondominant side at 180 deg/sec, 180 and 300 deg/sec respectively, mean peak torque of internal rotators were significantly high in dominant side at 300 deg/sec. 2) In nonpitchers, peak torque ratios of external/internal rotators were significantly high in nondo minant side at 180 deg/sec, but peak torque and peak torque body weight ratios of internal rotators were both significantly high in dominant side at 180 and 300 deg/sec. 3) In comparison between pitchers and nonpitchers, peak torque ratios of external/internal rotators in dominant side were significantly high in nonpitchers at 180 and 300 deg/sec, but there were no significant differences in nondominant side. 4) In flexibility tests of external rotators, pitchers showed statistically significant tightness in external rotators than nonpitchers and control group in dominant side, but there were no statistically significant differences in nondominant side. In conclusion, pitchers showed weakness and tightness in shoulder external rotators, which may lead to major shoulder injury. Therefore evaluation of muscle power and flexibility was thought to be one of the very useful screening tests for the prevention of shoulder injury.