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Staphylococcus aureus와 Coagulase-Negative Staphylococcus Species에 대한 Arbekacin의 시험관내 항균력
위성헌,강진한,허동호,이동건,김상일,김양리,최정현,김종현,유진흥,허재균,신완식,강문원 대한감염학회 2001 감염 Vol.33 No.4
Background : Most strains of methicillin-resistant Staphylococcus aureus (MRSA) now exhibit high-level resistance to various antibiotics, such as β -lactam antibiotics, aminoglycosides, macrolides, tetracyclines and quinolones. Recent reports describing the therapeutic failure of vancomycin for MRSA infections have arisen considerable concerns regarding the emergence of MRSA strains, which will require new therapeutic agents. Arbekacin, an aminoglycoside antibiotic, has antibacterial activity against both gram-positive and gram-negative bacteria and is stable in the presence of aminoglycoside inactivating enzymes produced by S. aureus. In this study, we compared the antibacterial activity of arbekacin with those of vancomycin, gentamicin, and amikacin against Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CNS). Methods : For a collection of 549 S. aureus and 251 CNS isolates from three Catholic University Hospitals in Korea, minimum inhibitory concentrations (MICs) of arbekacin, vancomycin, amikacin and gentamicin were determined by agar dilution method using Mueller-Hinton agar according to NCCLS (National Committee for Clinical Laboratory Standards, USA)criteria. Results : Among 549 S. aureus isolates, 278 isolates were MRSA and 271 isolates were methicil sensitive S. aureus (MSSA). MIC50 & MIC90 of arbekacin against 549 S. aureus were 0.5 & 1 ㎍/mL, and MIC50 & MIC90 of vancomycin were 1 & 1 ㎍/mL. MIC of arbekacin against 549 S. aureus isolates ranges from 0.03 to 4 ㎍/mL, and MIC of vancomycin against 549 S. aureus ranges from 0.25 to 2 ㎍/mL. MIC90 of amikacin against 549 S. aureus was 32㎍/mL, and that of gentamicin was 128 ㎍/mL. MICs of amikacin and gentamicin were variable, ranging from 0.125 to 256, and otherwise arbekacin and vancomycin revealed relatively narrow range of MICs. MIC90 of arbekacin against 278 MRSA isolates & 271 MSSA were 1 & 0.5 ㎍/mL, and those of vancomycin against MRSA & MSSA were 1 & 1 ㎍/mL. MIC90 of amikacin against 278 MRSA & 271 MSSA isolates were 32 & 4 ㎍/mL, and that of gentamicin against MRSA & MSSA isolates were 128 & 32 ㎍/mL respectively. Among 251 CNS isolates, 122 isolates were MRCNS and 129 were MSCNS. MICSO & MIC90 of arbekacin against 251 CNS isolates were 0.25 & 2 ㎍/mL, and those of vancomycin were 1 & 2 ㎍/mL. MIC of arbekacin against 251 CNS isolates ranges from 0.015 to 32 ㎍/mL, and that of vancomycin isolates ranges from 0.25 to 2 ㎍/mL, MIC90 of arbekacin against 122 MRCNS & 129 MSCNS isolates were 2&0.3 ㎍/ML, and those of vancomycin were 2&s ㎍/ML. MIC90 of amikacin against 251 CNS isolates was 32 ㎍/ML, and that of gentamicin was 128 ㎍/ML for CNS. MIC90 of amikacin against 122 MRCNS & 129 MSCNS isolates were 128 & 8㎍/mL, and those of gentamicin ere 256 & 32 ㎍/mL. Conclusion : Considering above results, arbekacin can be useful agent against most strains of MRSA and MRCMS, which exhibit high-level resistance to amikacin and gentamicin. (Korea J Infect Dis 33:254~260, 2001)
Alteration of The Quaternary Structure of Human UDP-Glucose Dehydrogenase by a Double Mutation
( Jae Wan Huh ),( Seung Ju Yang ),( Eun Young Hwang ),( Myung Min Choi ),( Hyun Ju Lee ),( Eun A Kim ),( Soo Young Choi ),( Jene Choi ),( Hea Nam Hong ),( Sung Woo Cho ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.5
Alteration of The Quaternary Structure of Human UDP-Glucose Dehydrogenase by a Double Mutation
Huh, Jae-Wan,Yang, Seung-Ju,Hwang, Eun-Young,Choi, Myung-Min,Lee, Hyun-Ju,Kim, Eun-A,Choi, Soo-Young,Choi, Jene,Hong, Hea-Nam,Cho, Sung-Woo Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
There are conflicting views for the polymerization process of human UDP-glucose dehydrogenase (UGDH) and no clear evidence has been reported yet. Based on crystal coordinates for Streptococcus pyogenes UGDH, we made double mutant A222Q/S233G. The double mutagenesis had no effects on expression, stability, and secondary structure. Interestingly, A222Q/S233G was a dimeric form and showed an UGDH activity, although it showed increased $K_m$ values for substrates. These results suggest that Ala222 and Ser233 play an important role in maintaining the hexameric structure and the reduced binding affinities for substrates are attributable to its altered subunit communication although quaternary structure may not be critical for catalysis.
( Jae Hyun Bae ),( Yong Ho Lee ),( Yu Jung Yun ),( Gyuri Kim ),( Eun Yeong Choe ),( Kyu Ha Huh ),( Yu Seun Kim ),( Byung Wan Lee ),( Chul Woo Ahn ),( Bong Soo Cha ),( Hyun Chul Lee ),( Myoung Soo Kim 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Dipeptidyl peptidase-4 (DPP-4) inhibitors have widely used in kidney- transplant patients with diabetes. Some of them are metabolized via cytochromeP450 pathway, which is also responsible for the immunosuppressants metabolism. However, there are no suffi cient studies regarding drug interactions between DPP-4 inhibitors and immunosuppressants. The aim of this study was to compare the effect of three DPP-4 inhibitors on blood cyclosporine levels in kidney-transplant recipients with diabetes. Methods: A total of 46 renal allograft recipients with diabetes who began to take DPP-4 inhibitors and cyclosporine after transplantation were retrospectively analyzed. Changes in blood cyclosporine levels were assessed in patients treated with one of three DPP-4 inhibitors, vildagliptin, sitagliptin, and linagliptin for two months. The glucose-lowering effi cacy of three DPP-4 inhibitors were also compared using glycosylated hemoglobin (HbA1c) after treatment for three months. Results: Blood cyclosporine level was signifi cantly increased in the sitagliptin group (19.27 ± 30.76 ng/mL), compared with vildagliptin and linagliptin group after two months of treatment (-21.0 ± 27.76 ng/mL, and -8.64 ± 40.76 ng/mL, p=0.011). After adjustment with multiple comparison, difference between vildagliptin and sitagliptin group was statistically signifi cant (p = 0.01). There were no signifi cant differences in HbA1c levels among vildagliptin, sitagliptin, and linagliptin treatment (0.04 ± 1.05% vs. -0.17 ± 0.54% vs. -1.19 ± 1.46%, p=0.115). Conclusions: Blood cyclosporine levels may be increased with sitagliptin co-treatment. Physician should consider drug interaction when prescribing sitagliptin in kidney-transplant patients receiving cyclosporine as an immunosuppression. Further investigation with a larger sample and long-term follow-up data is needed.
Wan Soo Kim,Jong Wook Kim,Jae Hak Lee,Sung Hoe Huh 한국해양과학기술원 2008 Ocean science journal Vol.43 No.2
Laboratory bioassays were conducted to test the acute toxicity effects of sudden exposure to sodium cyanide (NaCN) on the endogenous rhythm of the oxygen consumption rate (OCR) in the black rockfish Sebastes schlegeli. The OCR of the black rockfish (n = 14, total length = 20.4 ± 1.16 cm, wet weight = 158 ± 25 g) was measured with an automatic intermittent-flow-respirometer. OCR decreased significantly when experimental fish were exposed to NaCN. When exposed to 10 ppb NaCN, fish were able to recover their OCR rhythmic activities. When fish were exposed to 20 ppb, however, the metabolic activity rhythms were not recovered. These results suggest that exposure to NaCN concentrations over 20 ppb cause severe physiological damage to the endogenous rhythms of black rockfish.
Huh, Jung-Bo,Lee, Jeong-Yeol,Jeon, Young-Chan,Shin, Sang-Wan,Ahn, Jin-Soo,Ryu, Jae-Jun The Korean Academy of Prosthodonitics 2013 The Journal of Advanced Prosthodontics Vol.5 No.2
PURPOSE. The aim of this study was to evaluate the stability of arginine-glycine-aspartic acid (RGD) peptide coatings on implants by measuring the amount of peptide remaining after installation. MATERIALS AND METHODS. Fluorescent isothiocyanate (FITC)-fixed RGD peptide was coated onto anodized titanium implants (width 4 mm, length 10 mm) using a physical adsorption method (P) or a chemical grafting method (C). Solid Rigid Polyurethane Foam (SRPF) was classified as either hard bone (H) or soft bone (S) according to its density. Two pieces of artificial bone were fixed in a customized jig, and coated implants were installed at the center of the boundary between two pieces of artificial bone. The test groups were classified as: P-H, P-S, C-H, or C-S. After each installation, implants were removed from the SRPF, and the residual amounts and rates of RGD peptide in implants were measured by fluorescence spectrometry. The Kruskal-Wallis test was used for the statistical analysis (${\alpha}$=0.05). RESULTS. Peptide-coating was identified by fluorescence microscopy and XPS. Total coating amount was higher for physical adsorption than chemical grafting. The residual rate of peptide was significantly larger in the P-S group than in the other three groups (P<.05). CONCLUSION. The result of this study suggests that coating doses depend on coating method. Residual amounts of RGD peptide were greater for the physical adsorption method than the chemical grafting method.
허재완 延世大學校經濟硏究所 1996 延世經濟硏究 Vol.3 No.2
그 동안 지방교부세제도는 재정력 불균형의 조정기능 및 일정행정수준 유지를 위한 재정보장기능에 중점을 두어 왔으며 상대적으로 징세노력 유발기능에는 별다른 관심을 기울이지 않았다. 그러나, 취약한 지방재정을 강화하기 위해서는 우선적으로 지방자치단체들이 지방예산을 절약하거나 징세노력을 강화하는 등 건전한 지방재정운영을 위한 자구노력을 기울여야 한다. 본 연구의 기분 목적도 지방교부세의 지방자주재원 보장 및 재원 형평화 기능이라는 본래 취지를 살리면서 자구노력을 하는 자치단체에 '인센티브'를 부여하고 징세노력 정도를 지방교부세 결정에 적용하는 보다 현실적인 방법을 제안하고 이의 유용성을 실증분석을 통하여 검증하는데 있다.