일부 산모에서 산모혈, 제대혈, 모유 및 태반에서의 환경 에스토레겐성 화합물(PCBs) : Environmental estromgen compounds(PCBs) in Maternal Blood, Cord Blood, Human Milk and Placenta of Korea women

이강숙,임현우,노영만,구정완,조재천,박정일,김진홍 가톨릭대학산업의학센타 산업의학연구소 2003 韓國의 産業醫學 Vol.42 No.2

The production and intensive use of organochlorine chemicals, both in agriculture and industry, have led to wide spread contamination of the environment. There may be biological effects of organochlorine contaminated breast milk even when levels are below those concentrations that are currently regarded as tolerable. To evaluate the exposure of to fetus PCBs, we measured the sum of 13 congeners of PCBs in maternal blood, cord blood, breast milk and placenta of 30 Korean women by gas chromatograpy/electron capture detector. The concentration of total PCBs of maternal blood, cord blood and breast milk were 7.16±4.29 ㎍/ℓ, 5.16±3.12 ㎍/ℓ, 1.77±2.87 ㎍/ℓ respectively and they have no significant correlation. PCBs in placenta was not defected. However there were no significant differences of PCBs according to age, education, body mass index, parity, breastfeeding, smoking and drinking.

간내 간충병에 대한 간절제술 1예

심민철,김홍진,권광보,노성균,이헌주,이태숙,장재천 영남대학교 의과대학 1990 Yeungnam University Journal of Medicine Vol.7 No.1

영남대학교 의과대학 외과학교실에서는 1989년 11월에 한국에서 발병한 간흡충병의 여섯 번째 증례로, 특히 간내 담관에 발생한 첫 증례를 우측 간엽 절제술 및 담낭 적출술로 좋은 결과를 얻었으며, 간내 담관에 발생한 경우에는 간암과의 구별이 요구되어야 한다는 점을 상기 시키면서 증례보고와 더불어 문헌적 고찰을 하였다. Human Fasciola hepatica infection is a rare entity involving infestation of the liver and biliary tree with adult flukes, which can result in hepatitis, cirrhos is and biliary tract inflammation, obstruction and lithiasis. The patient had the typical diagnostic tetrad of fever, eosinophilic leukocytos is, tender hepatomegaly and fluke ova in the stools. Theatment consistes of Emetine hydrochloride administration for hepatic involvement and common bile duct exploration for removal of flukes, with cholecystectomy for associated cholelithiasis. The combination of medical and surgical therapy cal be expected to produce an arrest of this infection. The removed liver revealed eggs of the fasciols species in the intrahepatic bile duct. The clinical history, pathological findings and treatment of this case were described.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

Regulatory Perspectives on Pharmacogenomics

( Jae Sook Roh ) 한국응용약물학회 2006 학술대회 Vol.2006 No.2

난소내 혈관상피성장인자 ( Vascular Endothelial Growth Factor ) 의 발현 양상

노재숙(Jae Sook Roh),강지연(Ji Yeon Kang),지일운(Ill Woon Ji),정은환(Eun Hwan Jeong),안치석(Chi Seok Ahn),김학순(Hak Soon Kim) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.12

N/A Objective: The ovarian cycle is characterized by repeating patterns of cellular proliferation and differentiation that accompany follicular development and the formation and regression of the corpus luteum (CL). That angiogenesis may play an important role in this process. Angiogenesis is supposed to be regulated by vascular endothelial growth factor (VEGF). The goal of the present investigation, therefore, was to determine whether the expression of VEGF was changed in the normally cycling human ovary. We also investigated VEGF expression in the regressed CL (ie, nonfunctiong CL) of normal term pregnancy to define the association with steroidogenic activity. To our knowledge there is no report available on VEGF expression in the CL of term pregnancy. Methods: We assessed VEGF expression in ovaries obtained from, 26-42 yr of age, and from patients undergoing hysterectomy and salpingo-oophorectomy for nonendocrinological or nonovarian disorders. Tissue samples from premenopausal women included specimens from follicular (n=4) and luteal (n 4) phases. In addition, we studied ovarian specimens from pregnant women (n=3). Immunohistochemical analysis for VEGF was performed using a rabbit polyclonal antibody directed against human VEGF. Results: These data demonstrate a development-related VEGF expression in the follicle and indirectly show that VEGF expression may be up to the existence of LH-receptor. And also, VEGF was overexpressed in the regressed CL of pregnant women compared with the functioning CL of nonpregnant cycles Conclusion: This study suggests that the intensity of VEGF expression is not correlated with steroidogenic activity, although both of them are stimulated by LH.

Implication of Oxygen Level on the regulation of follicle growth in cultured mice ovaries in vitro

노재숙 ( Jae Sook Roh ),Teresa K Woodruff 대한산부인과학회 2008 대한산부인과학회 학술대회 Vol.94 No.-

Regulation of Wilms` tumor suppressor gene, WT1, FSH and FSH receptor in the immature rat granulosa cells(초)

노재숙 ( Jae Sook Roh ),( Mi Hyeon Jeong ) 대한산부인과학회 2009 대한산부인과학회 학술대회 Vol.95 No.-

진행성 자궁경관무력증에 있어서 응급자궁경부봉축술의 임상적 유용성

노재숙(Jae Sook Roh),정은환(Eun Hwan Jeong),김학순(Hak Soon Kim) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.12

N/A To evaluate the clinical efficacy of emergency cervical cerclage in the treatment of advanced cervical incompetence, a prospective clinical trial was performed in 18 patients with advanced cervical incompetence at 20 to 25 weeks gestation. Modified McDonalds operation with or without transabdominal amniocentesis was used for cervical cerclage. Pregnancy was prolonged for a sufficient time to deliver viable fetuses in 56.3% of patients. The median procedure-to-delivery interval was 8.6 (range 0.1 to 20.5) weeks for the entire group, and 13.9 (range 6.5 to 20.5) weeks for 9 patients who achieved live birth. The median gestational age at delivery was 38.6 (range 28.3 to 41.0) weeks for the live infants. The median birthweight of the live infants was 3062 g (range 1050 to 3620 g). The lack of significant matemal morbidity combined with the results for the fetus-infant supports further efforts in this area.

사람의 난관 상피세포에서 인슐린유사 성장인자 - II 의 발현

노재숙(Jae Sook Roh),성노현(Ro Hyun Sung),신중식(Joong Sik Shin),유중배(Jung Bae Yoo),황윤영(Youn Yeung Hwang) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.9

연구목적 : 인슐린유사성장인자-II가 여성의 자궁이나 난소조직의 주기적인 변화에 관여하는 것은 잘 알려져 있으나, 난관 상피세포의 주기적인 변화와도 관련이 있는지는 명확치 않다. 본 연구는 난관 상피세포에서 IGF-II가 발현되는지를 알아보고, 발현되는 경우 생식주기에 따른 차이를 보이는지 비교하였다. 연구대상 및 방법 : 다양한 부인과 질환으로 개복수술을 받았던 월경주기가 규칙적인 16명의 가임기 여성과 4명의 폐경기 여성에서 난관조직을 얻어 IGF-II에 대한 면역조직염색을 시행하였다. 연구결과: IGF-II는 주로 난관 상피세포에서 강하게 발현되었고 기질세포와 근육층에서는 약하게 발현되었다. 상피세포 중에서 섬모가 있는 세포와 없는 세포간에 차이는 없었다. 난관 상피세포에서 IGF-II의 발현정도는 생식주기에 따른 차이를 보여, 증식기 중,후기와 분비기 초,중기가 증식기 초기 및 분비기 후기와 비교하여 강하게 발현되고 폐경기에서는 발현되지 않았다. 결 론 : 본 연구결과 가임기 여성의 난관 상피세포에 IGF-II가 발현된다는 것을 알 수 있었다. 이는 IGF-II가 직, 간접적으로 난관 기능에 관여한다는 것을 의미하며, 또한 주기에 따른 발현 정도의 차이는 IGF-II가 난소에서 분비되는 스테로이드 호르몬의 영향을 받는다는 것을 의미한다. Objective : The involvement of IGF-II in reproductive tissues, especially uterine and ovarian, there is very little information is available concerning their presence in human tubal epithelium. To elucidate this, the present study was undertaken to determine the pattern of expression and cyclic changes of IGF-II in human tubal epithelium during various reproductive stages including postmenopause. Methods : Tube samples from 16 normally cycling women, as well as from 4 postmenopausal women were obtained. The expression pattern of IGF-II was examined using immunohistochemistry. Results : The primary site of immunoreactivity for IGF-II in fallopian tube tissue was in the epithelial lining of the tubes, with substantially lower intensity in the stromal cells and smooth muscle layer. The immunostaining was associated with both cilicated and nonciliated tubal epithelial cells without substantial differences in their intensity. Immunostaining intensity of IGF-II in tubal epithelial cells was cycle-dependent and considerably higher in mid-late proliferative and early-mid secretory compared to early proliferative and late secretory phases of the menstrual cycle, with no immunostaining in the postmenopausal period. Conclusion : This study demonstrates the presence of IGF-II in the human fallopian tube during various stages of the menstrual cycle. These data suggest an autocrine or paracrine role for the IGF-II in fallopian tube function, and their cyclic dependency further implies ovarian steroidal regulation.

Gene Expression Profiling of Non-Small Cell Lung Cancer

Mee Sook Roh,Hyuk Chan Kwon,Jin Sook Jeong,Dae Cheol Kim,Choon Hee Son,Soo Keol Lee,Phil Jo Choi,Jae Ik Lee,Ki Nam Lee,Hyo Jin Kim,Jin Han Yoon,Tae Ho Hwang 대한암학회 2003 Cancer Research and Treatment Vol.35 No.2

Purpose: cDNA microarray provided a powerful alternative,with an unprecedented view scope, in monitoringgene expression levels, and led to the discoveryof regulatory pathways involved in complicated biologicalprocesses. This study w as performed to gainbetter understanding of the molecular mechanismsunderlying the carcinogenesis and progression oflung cancer.Materials and Methods: Using a cDNA microarray,representing 4,600 cDNA clusters, we studied the expressionprofiles in 10 non-small cell lung cancer (NSCLC)samples and the adjacent noncancerous lung tissuesform the same patients. The alterations in the levelsof gene expression were confirmed by reverse-transcriptionPCR in 10 randomly selected genes.Results: Genes that were differently expressed in thecancerous and noncancerous tissues were identified.One hundred and nine genes (of which 68 were known)and 69 cDNAs (of which 32 were known) were up- anddown-regulated in 〉70% of the NSCLC samples, respectively.In the cancerous tissues, the genes related tothe cell cycle, metabolism, cell structure and signaltransduction, were mostly up-regulated. Furthermore,we identified a few putative tumor suppressor genesthat had previously been proposed by other workers.Conclusions: These results provide, not only a newmolecular basis for understanding the biological propertiesof NSCLC, but also useful resources for thefuture development of diagnostic markers and therapeutictargets for NSCLC. (Cancer Res Treat. 2003;35:154-160)