물류 원가 ABC 시스템을 이용한 다기준 활동 원가 분석 연구

양광모,김창식,박재현,강경식 한국물류학회 2006 물류학회지 Vol.16 No.1

黃蓮解毒湯의 製劑化 및 品質管理에 관한 硏究

양재헌,박현구,김영일 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

A traditional prescription, Hwangryean-haedog-tang(HRHT) was composed of Coptidis Rhizoma, Phellodendri Cortex, Scutellariae Radix, Gardeniae Fructus(1.5:1.5:3.2) and it has been used in the treatment of hypertension. insomnia, mental disorder, medicinal poisoning, crapulence. The precipitation was formed during the preparation of decoction of HRHT. This precipitatiion was very slightly soluble in water. Because this precipitated reaction decrease the effect of a gerbal medicine, the content of berberine and baicalin was determined in the precipitated product and supernatant. Berberine which active ingredient of Coptidis Rhizoma, Phellodendri Cortex was identified and determined by HPLC using u-Bondapak C_18 column with acetonitrile:pH 5.2 phosphate buffer(60:40v/v). Baicalin which active ingredient of Scutellariae Radix was identified and determined by HPLC using u-Bondapak C_18 column with 0.5% phosphoric acid:acetonitrile(73:27v/v). The content of erberine in the coprecipitated product and supernatant were 11.6% and 1.5% and the content of baicalin were 20.7% and 2.4% respectively. To ingibit the precipitated reaction of HRHT. Coptidis Rhizoma and Phellodendri Cortex were mixed and extracted whth hot water, it were made into gastric-coated granule, Scutellariae Radix and Gardeniae Fructus mixed and extracted, it were made into enteric0coated granule.

Effects of epigallocatechin gallate on CoCl_(2)-induced apoptosis in PC12 cells

Yang, Kyu-Ho,Mo, Hyun-Chul,Choi, Nam-Ki,Kim, Seon-Mi,Kim,Won-Jae 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Neuronal apoptotic events, consequently resulting in neuronal cell death, are occurred in hypoxic/ischemic condition. This cell death has been shown to be accompanied with the production of reactive oxygen species (ROS), which can attack cellular components such as nucleic acids, proteins and phospholipid. However, the underlying mechanisms of apoptosis induced in hypoxic/ischemic condition and its treatment methods are unsettled. Cobalt chloride (CoCl_(2)) has been known to mimic hypoxic condition including the production of ROS. Epigallocatechin gallate (EGCG). a green tea polyphenol, has diverse pharmacologial activities in cell growth and death. This study was aimed to investigate the apoptotic mechanism by CoCl_(2) and effects of EGCG on CoCl_(2)-induced apoptosis in PC12 cells. Administration of CoCl_(2) decreased cell survival in dose- and time-dependent manners and induced genomic DNA fragmentation. Treatment with 100 µM EGCG for 30 min before PC12 cells were exposed to 150 µM CoCl_(2), being resulted in the cell viability and DNA fragmentation being rescued. CoCl_(2) caused morphologic changes such as cell swelling and condensed nuclei, whereas EGCG attenuated morphologic changes by CoCl_(2). EGCG suppressed the apoptotic peak and a loss of Δψ_(m) induced by CoCl_(2). CoCl_(2) decreased Bcl-2 expression but Bax expression was not changed in CoCl_(2)-treated cells. EGCG attenuated the Bcl-2 underexpression by CoCl_(2). CoCl_(2) augumented the cytochrome c release from mitochondria into cytoplasm and increased caspase-8, -9 and caspase-3 activity, a marker of the apoptotic executing stage. EGCG ameliorated the incruement in caspase-8, -9 and -3 activity, and cytochrome c release by CoCl_(2). NAC (N-acetyl-cysteine), a scavenger of ROS, attenuated CoCl_(2)-induced apoptosis in consistent with those of EGCG. These results suggest, that CoCl_(2) induces apoptotic cell death through both mitochondria- and death receptor-dependent pathway and EGCG has neuroprotective effects against CoCl_(2)-induced apoptosis in PC12 cells. 신경세포자멸사는 저산소 및 허혈환경에서 일어나며 이러한 세포죽은 reactive oxidant species (ROS) 생성을 동반함이 알려져있다. 그러나, 저산소 및 허혈환경에서 일어나는 세포자멸사의 기전 및 그 치료방법은 아직 정립되어 있지 않다. CoCl_(2)는 ROS를 생성하는 등 저산소환경과 유사한 조건을 초래하는 것으로 알려져 있다. Epigallocatechin gallate (EGCG)는 녹차의 polyphenol 성분으로서 세포성장과 죽음에 다양한 약리학적 효과를 나타냄이 알려져 있다. 본 연구는 PC12 세포에서 CoCl_(2)에 의한 세포자멸사기전을 밝히고 이에 미치는 EGCG의 효과를 조사하는데 목적이 있다. Cell viability는 MTT 측정으로 조사되었고, DNA fragmentation은 DNA laddering으로 조사되었다. Bcl-2와 Bax발현 정도는 RT-PCR로, caspase-3와 -9의 활성은 spectrophotometer, caspase-8의 활성은 flow cytometry에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 western blot으로, 분해된 DNA 양과 미토콘드리아 세포막전위 (Δψ_(m))는 FACScan으로 조사되었다. CoCl_(2)투여로 PC12 세포수는 용량 및 시간 의존형태로 감소하였고, genomic DNA fragmentation이 발생하였다. CoCl_(2)투여로 야기된 cell viability의 감소와 DNA fragmentation은 EGCG 전처치에 의해 억제되었다. CoCl_(2)은 세포용적팽창과 condensed nuclei 같은 형태적 변화를 일으켰으며, apoptotic peak, Δψ_(m)감소 및 cytochrome c 유리를 야기하였다. EGCG는 CoCl_(2)에 의한 세포형태변화, apoptotic peak, Δψ_(m)소실 및 cytochrome c 유리를 억제시켰다. CoCl_(2)는 Bcl-2 발현을 감소시켰지만, Bax 발현에는 영향을 미치지 않았다. EGCG는 CoCl_(2)에 의해 야기된 Bcl-2 발현 감소를 억제시켰다. CoCl_(2)는 caspase-3, -8, 그리고 -9의 활성을 증가시켰으며, EGCG는 CoCl_(2)에 의한 세포자멸사를 억제시켰다. 본 실험결과는 PC12 세포에서 CoCl_(2)가 미토콘드리아 의존 및 death receptor의존 기전으로 세포자멸사를 일으키며, EGCG는 세포자멸사기전을 억세지킴으로 신경보호기능을 가짐을 시사하였다.

공리설계를 적용한 고객관리 시스템 구축에 관한 연구

양광모,박재현,강경식 明知大學校 産業技術硏究所 2002 産業技術硏究所論文集 Vol.21 No.-

With ever-change and increasingly competitive business environments, firms strive to employ a variety of marketing strategies and execution in order to survive in the market. such effects would be paid off in the right way only when management of the firms perform marketing activities focusing on long term effectiveness, which would drive company profits up and keep them for long. Demands of customers are being changed and varied. And in this circumstance, it become a main issue of management that the company should produce and sell products according to the customer demands. With these trends, each company has been concentrating effects on generalization of product development technique and distinction of service for customer. In this result with the advantage of mass marketing and database marketing have been drawing attentions from company. Also the internet connected around the world completely diminished the limit of time and distance and company have enveloped keen competition out of each nation and continent in the world market. To fulfill these demands of customer, they need a concept of eCRM(Web based Customer Relationship Management), and go from selling products and services, or gathering customer requests, up to the phase of solving customer's problem by real time or previous action, With the help of internet, the frequency and speed of the problem solving has improved greatly. In this paper provide concept of eCRM and Axiom Design. And with the target company of study, it was built eCRM system using Axiom design to insert design for customer request.

^40Ca(d,p)^41Ca 직접 핵반응에서의 결합채널방정식에 의한 편극율 계산

양재춘,김성현 충남대학교 기초과학연구소 1995 忠南科學硏究誌 Vol.22 No.2

The polarization ratio of ^40Ca(d,p)^41Ca direct reaction was calculated by coupled channel method with Woods-Saxon optical potential. And the polarization ratio for incident particle energy of 10.8 and 20.8 MeV were calculated in the ground state f and first excited state p of ^41Ca nucleus respectively. The result of coupled channel method was compared with experimental data and a result of distorted wave Born approximation method. It was found that the result of calculation by coupled channel equation agreed with the experimental data and that it was shown CC calculation to be better than DWBA calculation.

QUAL2E모델에 의한 대청댐 상류하천의 수질예측

양현주,허재영 大田大學校 産業技術硏究所 1994 산업기술연구소 論文集 Vol.1994 No.8

The water quality in the Taechong dam basin was simulated for some conditions of flow and pollutant load with QUAL2E model verified to be available in several examples. The changes of water quality in 1996, 2001 and 2006 were predicted to evaluate the pollutant load contributions and aid to establish the water quality control plan. The flow rate for environment maintenance of upstream basin of Kum river was also proposed.

혐기-무산소-호기공정에 의한 도시하수의 질소·인제거에 관한 연구

양용운,김재현 啓明專門大學 産業開發硏究所 1999 啓明硏究論叢 Vol.17 No.2

본 연구는 질소와 인을 동시에 제거할 수 있는 생물학적 질소-인제거 공정인 혐기-무산소-호기공정을 산업폐수가 포함된 도시하수에 적용한 경우의 유기물 및 질소·인의 제거특성에 대하여 조사하였다. 원수의 특성은 유기물의 농도가 CODCr 120∼230mg/ι로 평균 178mg/ι을 나타내었으나 무기성 탄소원의 농도가 매우 높았으며 이에 따라 생물학적 제거 가능한 유기물의 비율은 낮아 충분한 질산화와 탈질화가 이루어지지 않았다. CODCr/NH_(3)-N이 평균 10이상으로 높았지만 무기성 탄소원이 높아 탈질에 필요한 추가적인 에너지원의 투입을 고려해야 하며, 또한 alkalinity/NH_(3)-N의 비가 2.3정도로 낮게 나타나 완전한 질산화를 위해서는 alkalinity의 공급이 필요한 것으로 조사되었다. 유기물에 대한 평균 제거율은 BOD 84%, CODCr 53%를 나타내었으며, NH_(3)-N의 제거율은 평균 76%이었고 T-N의 제거율은 60%이었다. 그리고 T-P 제거율은 50%정도를 나타내었다. The anaerobic-anoxic-aerobic process was used for biological nitrogen and phosphorus removal of municipal sewage. The CODCr concentration of municipal sewage was 120∼230mg/ι and its average CODCr concentration was 178mg/ι. But because of the high inorganic concentration of municipal sewage, the biological nitrification and denitrification were not accomplished successfully. The alkalinity/NH_(3)-N was 2.3 and under those conditions the additional carbon source and lime should be added The removal efficiency BOD and CODCr were 84% and 53% respectively. The removal efficiencies T-N and T-P were 60% and 50% respectively.

QFD 전개를 이용한 전자파 차단도료 고객만족도 평가

양광모,박재현,강경식 明知大學校 産業技術硏究所 2002 産業技術硏究所論文集 Vol.21 No.-

Developing a new commercial product, it is need to connect the end users demand of quality to the industrial technology of company. for the reason, this study is to build up the users demand for imminent marketing product of a certain company by AHP(Analytic Hierarchy process), analyse quantitatively users subjective thoughts collected by Group Consensus, calculate the added-value of users demands and verify the consistency of users opinions by consistency-exponential-calculation. The added value obtained by this method is substituted into a user-demand item of QFD(Quality Function Development). and, the technical characteristic data transferred from the extracted essential factor for developing and manufacturing a new product is substituted into a technical characteristic item of QFD. The faculty of quality is firstly finished by this procedure. But, because the relation a technical characterization with users demand do not be known in new product, Wassermans method was introduced for correlation users demand with technology and for the processing and marketing of a new product. The all assumption on the thesis was based on the reliable real data of a certain company.

김치저장성 향상을 위한 동결방법이 배추조직의 물성변화에 미치는 영향

양진한,박소희,유진현,임호수,조재선,황성연 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.2

김치를 10˚C에서 8일간 숙성시켜 산도 0.6~0.7%로 숙성된 김치를 -70˚C와 -20˚C로 냉동하며 -20˚C에서 저장하면서 배추 조직의 elasticity, hardness, 세포 조직의 변화, 드립양을 실험한 결과는 다음과 같다. -70˚C에서 급속 동결한 것과 -20˚C에서 완만 동결한 냉동 김치 중 배추조직의 elasticity는 냉동 저장 15일까지 감소하다 일정하게 유지되었고 hardness는 거의 변화가 없었으며 냉동 방법에 따른 변화도 거의 나타나지 않았다. 투과 전자현미경으로 관찰한 결과 control의 경우 세포벽이 매우 두꺼우며 세포의 모양들이 잘 보존되어 잇는 것을 볼 수 있었고 -20˚C로 냉동 처리하여 해동시킨 세포벽들은 많이 손상되어 있음을 볼 수 있었으며 -70˚C로 급속 냉동시료의 경우 세포벽의 손상 정도가 -20˚C로 냉동 처리한 시료보다 덜 파괴되어 있음을 볼 수 있었다.한편 냉동 저장기간 동안 드립의 손실량의 변화는 -70˚C로 냉동 처리한 시료의 드립양은 3~4%정도로 -20˚C로 냉동 처리한 시료의 5~6%에 비해 적은 것을 알 수 있었다. This study was conducted to investigate the effect of freezing methods, show freezing at -20℃ and rapid freezing at -70℃, on physical properties of Chinese cabbage in frozen Kimchi during storage at -20℃. Elasticity of midrib of Chinese cabbage in frozen Kimchi was decreased until 15 days and did not changed thereafter during storage at -20℃, Hardness of that was not changed during storage. Those results of elasticity and hardness of slow frozen sample are similar to rapid frozen sample. By the morphological observation through transmission electron microscope, more of cellular structure of Chinese cabbage in slow frozen was destructed than that of rapid frozen sample. Drip loss was more in slow frozen sample than that in rapid one.

Kainic Acid 투여에 의한 흰쥐 해마형성체에서의 c-fos 및 Enkephalin 유전자 발현에관한 연구

양재승,김현,고재필,서영석 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3