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중이온 빔 조사에 의한 담배의 돌연변이 유도와 내염성 식물의 선발
배창휴,Abe, Tomoko,민경수,김동철,정재성,이춘환,임용표,이효연 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-
중이온 빔을 이용한 방사선 돌연변이의 기초자료를 얻고자 담배의 수분·수정 직후의 원배에 100 Gy의 중이온빔을 조사하여 M_1세대의 종자를 얻었다. NaCI 2.0과 2.5%를 포함된 MS배지에 M_1 세대의 종자를 파종하여 17개통의 NaCI 저항성 식물체를 선발하였다. 저항성 식물체는 BY-4품종에 ^14N빔을 조사한 처리구에서만 관찰되었다. M_1 세대의 저항성 식물체의 경우 영양생장기간 중에는 외형적 변이가 관찰되지 않았으나 생식생장기의 화기에 있어서는 다양한 변이가 관찰되었다. 특히 암술 수술의 길이의 변화, 꽃잎의 변화, 불완전 화분립, 줄기당 꽃의 착생수 감소 등의 변이가 발생하였다. M_1 세대에서 17개의 NaCI저항성 계통 중에서 8계통은 M_2 세대에도 저항성형질이 발현되었고, 또한 M_2 세대의 3계통은 Mannitol이 포함된 배지에서 저항성을 보여주었다. Tobacco proembryos were irradiated with 100 Gy of heavy-ion beams (^14N, ^20Ne : 135 Mev/u) after 24 to 96 hours of pollination as a mutagen and screened M_1 generation for morphological mutants and salt-tolerant plants. Morphological and physilolgical characteristics of the salt-tolerant plants derived from the irradiated proembryo are discussed in this report. Mutants irradiated porembryos with the beams after pollination produced various kinds of morphological variation. A total of 17 salt-tolerant plants were selected from tobacco cultivar (BY-4) by treatment with ^14N beam. Shapes of filament and pollen grain of most salt-tolerant plants were abnormal compared with non-irradiated wild type, and seeds weight and fertility obviously decreased. The germination rates of the several M_2 lines on the saline and the mannitol condition were higher than that of wild type.
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배향선,남철현,김성우,최연희,강영숙,김선혜,구재희 慶山大學校 保健福祉硏究所 2003 保健福祉硏究 Vol.8 No.-
This study was conducted to examine aspects of skin care of male students at colleges and universities of male students at colleges and universities and its related factors in order to provide informations on skin care for men. The subjects of this study was 600 men who were students at colleges and universities in Daegu City and Kyungbuk Province. Data were collected from May 1, 2002 to June 30, 2002. The results of this study can be summarized as follows. 1. According to the respondents' socio-demographic characteristics, 55.9% of them was 20 to 24 years old and 29.6% was more than 25 years old. 84.9% of them was university students, while 15.1% was college students. 25.3% belonged to the lower class, while 22.4% belonged to the upper class. 52.7% did not have a girl friend, while 47.3% has a girl friend. 41.8% lived in big cities and 48.1% lived in small and medium cities. 2. 63.0% of the respondents replied that they were in good health, while 8.8% of them were in bad health. 41.9% of them was concerned about face and body skin care, while 14.1% was not concerned about it. Among most serious skin problems, the proportion of acne was highest (44.5%). 3. Among the persons who advised the respondents when they selected cosmetics, the proportion of selecting cosmetics as their own will was highest (44.7%). 25.3% of them selected it by recommendation of female family members and 18.3% selected it by recommendation of the girl friend. In the case of selecting it as their own will, the older they were, the higher the proportion was. In the case of having the girl friend, the proportion of selecting cosmetics by recommendation of the girl friend was higher than that of selecting it by recommendation of female family members. 4. 90.4% of the respondents replied that they never visited skin care rooms, while only 9.6% of them replied that they ever visited them. In the case of visiting skin care rooms, the proportion of the upper class was higher than that of the lower class. 5. According to the frequency of visiting skin care rooms, the proportion of once a week was highest (51.0%). 29.4% of them visited them once per 6 months and 19.6% visited them once a month. 6. In the case of the reasons they did not visit the skin care rooms, the proportion of 'no interest' was highest (36.6%). 23.2% did not visit them because they thought the fee for skin care was expensive and 19.5% did not visit them because they thought the skin care room were only for women. 7. In the variables of giving advice concerning skin care, the proportion of 'their own' was highest (29.8%). The proportion of female family members was 28.2% and the proportion of the girl friend was 27.7%. In the case of the age related to having the girl friend's advice, the proportion of 'over 25 years old' was 31.9% and the proportion of '20 years old to 24 years old' was 26.4%. The proportion of 'less than 19 years old' was 23.5%. The older they were, the more they had the girl friend's advice. 8. According to the sources of getting information on skin care, the proportion of mass media (TV, Radio etc.) was highest (36.7%). 26.0% of them obtained them through the girl friend and 13.9% obtained them through female family members. Only 8.4% got them through magazines. 9. The factors influencing the experience of visiting skin care rooms were experience of side effect of foundation cosmetics, concern about skin care, expiration date of cosmetics or checking of directions of using cosmetics, donsideration of skin character when using soap, and regular exercise for health. From above results, it can be concluded that skin care and beauty specialists working in beauty academies or the department of cosmotology at colleges or universities must make efforts to change the negative recognition of male skin care through deep and comprehensive research. It is also necessary to develop systematic education course and institutionalize the qualification management system.
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신경섬유종증 환자에게서 발생한 특발성 혈소판 감소증 1예
권세훈,김형호,강지인,장재혁,원경준,장재현,김상용,배학연 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3
Idiopathic thrombocytopenic purpura is a disease characterized by thrombocytopenia in peripheral blood with positive anti-platelet antibody titer, no splenomegaly, the presence of megakarocyte. The causes of idiopathic thrombocytopenic purpura were usually related to autoimmune disease, infection and drugs. A 46-years-old man was admitted for evaluation of abdominal mass. He has many cafe-au-lait spots and nodules in almost skin for 30 years. Several years ago, he palpated mass in Lt. inguinal area. hIe has known the abdominal mass after checking abdomen CT in local clinic and visited our hospital for evaluation of abdominal mass. At admission, laboratory tests showed hemoglobin 12.5 g/dL, hematocrit 38.4 %, WBC 5,460 /㎣ (neutrophil 64.5%, lymphocyte 21.7%, monocyte 4.1%, eosinophil 5.4%), reticulocyte 0.7%, Platelet 52,000 /㎣, total protein 6.51 g/dL, albumin 3.80 g/dL, alkaline pohsphatase 161 IU/L, AST 110.7 IU/L, ALT 64.9 IU/L, total bilirubin 0.44 ㎎/dL, BUN 18.4 ㎎/dL, creatinine 0.79 ㎎/dL. Peripheral blood smear showed normocytic normochromic anemia, thrombocytopenia. Bone marrow aspiration smear showed normoplastic myeloid and erythroid precursor. The bone marrow biopsy section shows normocellular marrow (35%) for his age and mildly increasing myeloid cell lineage. We have experienced a case of idiopathic thrombocytopenic purpura in a patient being in neurofibromatosis and therefore reporting it with documentary records. 저자들은 신경섬유종증 환자에서 특별한 원인을 발견할 수 없었음에도 특발성 혈소판 감소증이 발생한 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다.
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사무직 근로자의 수면의 질 및 직무스트레스와 우울과의 관련성
강미나,강수영,권수정,김현주,배재원,이보연,이예진,임의롬,정다영,조한솔 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47
Purpose: The purpose of this study was to understand the degree of depression, sleep quality, job stress and their association among office workers. Method: A self-administered questionnaire was distributed to 147 workers employed in 5 big enterprises, and 5 small and medium enterprises from October 21 to November 21, 2012. The questionnaires included socio-demographic and health-related characteristics, job-related characteristics, sleep quality, job stress (KOSS) and depression (CES-D). Result: Among all subjects, 23.1% was in the depression group (21 points and over in the CES-D score). Depressive level was positively correlated with job stress and quality of sleep. The adjusted odd ratio for the effects of sleep quality and job stress on depression significantly increased in the depression group compared to that of the normal group. Multiple regression analysis revealed that the following factors of influence had combined explanatory powers on depression: BMI, life satisfaction, quality of sleep, and job stress. Conclusion: The study revealed that complicated influences were exerted on the level of depression by variable factors, as well as socio-demographic characteristics, health-related characteristics, quality of sleep, and job stress. Specifically, the level of depression was influenced by the quality of sleep and job stress.
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The Hornless Australian Burrowing Mayfly Ulmerophlebia (Ephemeroptera: Leptophlebiidae)
Bae, Yeon Jae,Finlay, Kyla J.,Campbell, Ian C. The Korean Society of Systematic Zoology 2012 Animal Systematics, Evolution and Diversity Vol.28 No.4
The hornless Australian burrowing mayfly genus Ulmerophlebia Demoulin (Leptophlebiidae) is revised based on comprehensive examinations of adult and larval material collected throughout Australia. Two new species [Ulmerophlebia deani n. sp. and U. minuta n. sp.] and three named species [U. annulata (Harker), U. mjobergi (Ulmer) and U. pipinna Suter] are included. The larva of U. deani can be distinguished by the moderately developed apicomedial expansion of gills and W-shaped markings on the abdominal terga. The male adult of U. minuta can be easily distinguished by the greatly reduced penes. Descriptions, diagnoses, line-drawings of key characters, material and distributional data, taxonomic remarks and adult and larval keys are provided.
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Identification of CM1 as a Pathogenic Factor in Inflammatory Diseases and Cancer
Bae, Se-Yeon,Kim, Hyem-In,Yu, Yeon-Sil,Lee, Na-Eun,Kong, Joo-Myoung,Kim, Hang-Rae,Hwang, Young-Il,Song, Yeong-Wook,Kang, Jae-Seung,Lee, Wang-Jae The Korean Association of Immunobiologists 2011 Immune Network Vol.11 No.3
Background: CM1 (centrocyte/-blast marker 1) was defined by a mAb against concavabalin-A (ConA) activated PBMC. It is expressed in germinal center of human tonsil and on the surface of activated PBMC as well as cancer cells. Recently, increased productions of pro-inflammatory mediators were detected from activated PBMC by CM1 ligation. Methods: However, there is a limitation to explain the exact role of CM1 on inflammation and its related mechanisms, since the identity of CM1 is still not clarified. In our previous study, we have already confirmed that soluble form of CM1 was produced by Raji. Therefore, we performed Q-TOF analysis after immunoprecipitation of concentrated Raji culture supernatant using anti-CM1 mAbs. Results: As a result, we found that CM1 is identical to enolase-1(ENO1), a glycolytic enzyme, and we confirmed that results by silencing ENO1 using siRNA. It was also confirmed through competition assay between anti-CM1 and anti-ENO1 mAbs. Finally, we investigated the possible role of CM1 in inflammatory response and cancer. The ligation of CM1 on Raji cells with anti-CM1 mAbs induces the extensive production of prostaglandin $E_2(PGE_2)$. In addition, the increased activity of matrix metalloproteinase (MMP)-2/9 was shown in NCI-N87, stomach cancer cell line by CM1 stimulation. Conclusion: CM1 is identical to ENO1 and it might be an important role in the regulation of inflammatory responses.
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