일부 지역아동센터 아동에 대한 치과주치의 사업 3년간의구강건강상태, 진료 및 비용 분석

류재인 ( Jae In Ryu ),김용진 ( Yong Jin Kim ),박주현 ( Joo Hyun Park ),김현주 ( Hyun Joo Kim ),김종애 ( Jong Ae Kim ),정종일 ( Jong Il Jung ),홍민경 ( Min Kyeong Hong ),정세환 ( Se Hwan Jung ) 대한예방치과·구강보건학회 2014 大韓口腔保健學會誌 Vol.38 No.4

Objectives: This study aims to investigate the trends and progress in oral health status, dental serviceitems, and fees among children receiving community-based registered dental care over a three-yearperiod. Methods: The study subjects were selected from nine community children centers in J district of S city,in the Korean province of Gyeonggi-do. The sample included 222 children who had received care in2011 and 2012, and 205 children, in 2013. The dependent variables were oral health status (df indexand decayed-missing-filled teeth index), dental service items (total number of visits and dental fillingsby type), and dental fees (total fees, National Health Insurance [NHI] coverage, and NHI non-coverage),analyzed by year. The percentages of dental caries and dental service items were tested using chisquareanalysis, and the mean of each variable, including dental fees, was tested using the Kruskal-Wallis method, owing to non-parametric distribution. Results: There was a statistically significant decrease in the rate of dental clinic visits for treatment, andan increase in the mean numbers of filled teeth and sealants performed per year. The rate and numberof dental fillings increased steadily, whereas the services for oral health promotion and preventionwere decreased. The number of dental visits and the total fees decreased steeply, especially within thesecond half of the last measured year: around 90,000 earned within that time, compared to 170,000earned during the first year. Conclusions: Dental clinic visits should be encouraged on a regular basis for oral health promotion andprevention by both patients and providers using capitation payment systems, for example. It is necessaryto monitor and provide training for all related staff by developing a manual for oral health examinationand treatment, adjusted for the registered dental system. Policy measures addressing the needsof vulnerable social groups are needed more than ever. Therefore, it is important to provide as muchtargeted support and training to the registered dental system as possible.

베체트병에서 비스테로이드성 소염진통제에 의해 발생한 대량 소장출혈 1예

강인숙,류연주,장지은,정성애,이지수,조영주,이경은,이지희 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2

Bechet's disease(BD) is a chronic inflammatoroy condition involving several organs inclu-ding gastrointestinal tract. Gastrointestinal tracts involvement in BD has been identified throughout the entire alimentary tract and commonly accompanies ulcerative lesions in the small and large bowel. It is debatable whether BD could be included among seronegative spondyloarthropathy (SPA).SPA usually occurs without overt sign of intestinal inflammation, but significant number of patients have asymptomatic intestinal inflammation, usually affecting ileum. Since most patients with SPA in-cluding BD are treated with NSAIDS. However, NSAID may play a role in aggravation or provo-cation of intestinal inflammation. Special attention to asymptomatic intestinal inflammation is needed, especially when NSAIDs are used for management of arthritic symptom in SpA. We experienced a case of BD which was complicated by a massive small bowel bleeding precipitated by NSAID use. 저자들은 베체트병 환자에서 비스테로이드성 소염진통제 사용 후 발생한 대량 소장 출혈의 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

Pollinating Characteristics of Apis mellifera and Bombus terrestris on Mango Flower in Vinyl-House Condition

In Gyun Park,Mi Ae Kim,Hyung Joo Yoon,Kyeong Yong Lee,Kyeong San Choi,Yoon Mi Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

To substitute for bluebottle fly, Chrysomyia megacephala which is being used for pollinator in mango fruit, and improve the pollinating effect of mango fruit which is also being increased as high value added crop recently in Jeju island of korea, 2 kinds of pollinator were used in analyzing and surveying of foraging activities on mango fruit in Seogwipo province. This study was conducted using 3 species of pollinator, Apis mellifera, Bombus terrestris and Chrysomyia megacephala with 3 treatment in vinyl-house condition respectively. Species of mango fruit, Irwin, was used in this experiment. A number of foraging activity of Apis mellifera and Bombus terrestris in hive showed highest 11 AM, and showed normal foraging activity in high temperature condition(28℃). Pollinating ratio of Bombus terrestris was shown 100% and over 95% in case of Apis mellifera. This ratio suggest that the 2 species of insects is effective as pollinator on mango fruit compared with bluebottle fly. Daily pollinating activity of Apis mellifera and Bombus terrestris was shown peak in 11 AM, but showed even activity from 9 AM to 3 PM in case of Chrysomyia megacephala. The pollinating characteristics of 3 species depends on illuminance but temperature, especially in case of Bombus terrestris was more affected by change of illuminance. Visiting time of Bombus terrestris and Apis mellifera on this flower was shown 2.8 and 3.4 seconds respectively. But Chrysomyia megacephala showed longer 10 times with 32.5 seconds than other insects. This results suppose that Chrysomyia megacephala showed as resting behavior for almost time on the flower not foraging activity to pollinate.

Characteristics of Foraging Activities by 2 Species of Pollinator, A. mellifera and B. terrestris on Apricot Tree

In Gyun Park,Mi-Ae Kim,Hyung Joo Yoon,Kyeong Yong Lee,Yun Mi Kim 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.10

To increase the fruit quality and productivity of the apricot, 2 species of insects, Apis mellifer, and Bombus terrestris were used for pollination in apricot field in Yeongcheon province. This study was conducted using 3 kinds of methods for pollination effect, natural condition, net-house treatment and control method. Also this experiment carried out in 2 places, vinyl-house and field condition. In case of field treatment of Apis mellifera, heating lamp experiment was added in net-house to find out the reason for harmful efficacy of low temperature early in the spring. Species of apricot, Mangum, was used in this experiment. A number of foraging activity of Apis mellifera and Bombus terrestris in vinyl-house showed higher than in field condition. But after stoping heating, showed low efficacy after showing peak in 1 PM. In field, Apis mellifera showed peak of foraging activity from 1 to 3PM, heating lamp experiment of field condition showed peak in 3 PM and then lowest tendency to evening suddenly. The fruit set rate of Apis mellifera and Bombus terrestris in vinyl-house showed higher with 92% and 89% respectively than control with 83%. In field, heating lamp treatment of Apis melliferawas was highest with 28% compared to Bombus terrestris with 25% and control with 15%. This results suppose that apricot in field was to be harmed by low temperature and frost in blooming time early in spring

Cold Temperature and Humidity for Diapauses-Break of the Bumblebee Bombus terrestris

Hyung Joo Yoon,Kyeong Yong Lee,Mi Ae Kim,In Gyun Park 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

Bumblebees are widely used to pollinate various crops, especially tomato, in greenhouses and fields. An artificial hibernation is essential for year-round rearing of the bumblebee that undergoes one generation per year. Here, we investigated whether a cold temperature and humidity affect artificial hibernation of the bumblebee queen Bombus terrestris. In cold temperature regimes of 0℃, 2.5℃, 5℃, 7.5℃ or 12.5℃ under a constant humidity 70% over, the queens stored at 2.5℃ was the best performance in survival rate, which was 74.0% in one month, 67.0% in two months, 60.0% in three months, 46.0% in 4 months, 33.0% in 5 months, and 24.0% in 6 months and lowed in the order of 0℃, 5℃, 7.5℃ and 12.5℃. And also, the colony developmental characteristics after diapause was 1.2-1.5 fold higher than those at queens stored at 5℃. In terms of cold humidity, the queens hibernated at 70% under 2.5℃ was the best performance in survival rate, which was 93.3±3.4% in one month, 83.3±0.0% in two months, 76.7±0.0% in 3 months and 36.7±12.1% in 5 months and lowed in the order of 50% and 90%. The rates of oviposition, colony foundation and progeny-queen production of queens hibernated at 70% were 80.8%, 30.8% and 30.8%, respectively. These values correspond to 1.7-3.3 fold that at queens stored at 50%. Therefore, 2.5℃ and 70% R.H. were the favorable cold temperature and humidity conditions for diapauses-break of B. terrestirs queen.

Breeding of the Korean Native Bumblebee, Bombus ignitus

Hyung Joo Yoon,Kyeong Yong Lee,Mi Ae Kim,Sang Mi Han,In Gyun Park 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

Many bumblebee species have declined in number in recent decades, particularly in developing regions. Widespread declines of bumblebee species threaten the pollination levels of both wildflowers and crops. Here, we investigated the body weight and colony-developmental characteristics of Korean native bumblebee (B. ignitus) queens collected from 2000 to 2010 for conservation of native bumblebees for breeding. The average weight of 6,852 queens was 0.77 ± 0.44 g. The weight of B. ignitus queens collected in 2005 was the greatest, 0.87 ± 0.12 g, which was 1.0–1.3-fold heavier than any other year. The average oviposition rate was 81.6 ± 10.7%, and 2004 showed the highest rate, 95.0%. This value corresponded to 1.1-1.6-fold increases over the queens collected in the other years. The average rate of colony foundation was 60.9 ± 11.0%. Queens in 2008 exhibited the best performance, 75.4%, which was 1.0-1.9-fold higher than the other years. The rate of progeny-queen production averaged 27.0 ± 9.4% (Fig. 5) and peaked in 2001 at 43.2%; this value was 1.1–4.7-fold higher than other years. The average number of queens produced and number of generations begotten by queens was 27.6 ± 10.1% and 4.8 ± 2.0, respectively. Queens in 2000 averaged 9 generations of offspring, which was 1.1-3.1-fold greater than other years. These results indicate that the colony-developmental characteristics of the collected queens changed significantly between 2000 and 2010. In addition, there was no correlation between body weight and number of queens collected, although body weight was affected by collection year. Since 2008, the colony- developmental characteristics of queens have worsened.

Current Status and Agriculture Utilization of Commercial Managed Insect Pollinators in Korea

Hyung Joo Yoon,Kyeong Yong Lee,Mi Ae Kim,In GyunPark 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Commercially managed bees are available for pollination services and are used in large commercial fields, small gardens, or enclosures such as greenhouses and screen houses. We investigated the use rate and number of commercial bees such as bumblebees, honeybees and mason bees for the pollination of 10 major horticultural crops and fruit trees in Korea. The use rates of bumblebees for 10 major horticultural crops and fruit trees were approximately 7.9% and 2.8% in 2009, respectively. The use numbers of bumblebees as pollinators was more than 64,345 colonies which included 51,400 for 10 major horticultural crops and 12,945 colonies for 10 major fruit trees, in 2009. The use rate of honeybees as a pollinator for 10 major horticultural crops in greenhouses and fruit trees were approximately 48.0% and 7.7%, respectively. The number of hives used for 10 horticultural crops and fruit trees was estimated to be 305,216 and 32,386, respectively. The number of honeybees hives used for pollination of 10 major horticultural crops and fruit trees was estimated to be 337,602. The use numbers of honeybees as pollinators was more than 337,602 hives in 10 major horticultural crops and fruit trees, in 2009. The number of honeybee hives used as pollinators of outside crops, including many fruits and vegetables, was estimated to be more than 500,000. The value of honeybees as pollinators was estimated to be more than 8.5% to 15.0% of total Korean beekeeping products. The rate of use of mason bees for 10 major fruit trees was approximately 3.3%. The number of mason bees individuals used for 10 major fruit trees was estimated to be 1,350,000. The value of commercial insect pollinators in 2009 in Korea was estimated at more than $45 million.

Review : Agricultural Utilization and Year-Round Rearing Techniques of Bumblebees in Korea

( Hyung Joo Yoon ),( Kyeong Yong Lee ),( Mi Ae Kim ),( In Gyun Park ) 한국잠사학회 2011 International Journal of Industrial Entomology Vol.22 No.2

Commercially managed bees are available for pollination services and are used in large commercial fields, small gardens, or enclosures such as greenhouses and screen houses. This paper describes the current status and agricultural utilization of commercially managed bumblebees as well as bumblebee rearing techniques in Korea. We surveyed the use rate and number of bumblebees for the pollination of 10 major horticultural crops and fruit trees in Korea; in 2009, the use rates were approximately 7.9% and 2.8%, respectively. The use number of bumblebees as pollinators was more than 64,345 colonies, which included 51,400 colonies for 10 major horticultural crops and 12,945 colonies for 10 major fruit trees in 2009. The value of bumblebees as pollinators in 2009 in Korea was estimated at more than 5,100,000,000 won. We also describe feeding, rearing room conditions, colony initiation, mating and diapause breaks to establish year-round mass rearing techniques of the bumblebee Bombus ignitus.

Diagnostic Mutational Analysis of MECP2 in Korean patients with Rett syndrome

Kim, In-Joo,Kim, Yeon-Joo,Son, Byeong-Hee,Nam, Sang-Ook,Kang, Hoon-Chul,Kim, Heung-Dong,Choi, Ook-Hwan,Yoo, Mi-Ae,Kim, Cheol-Min 대한유전성대사질환학회 2005 대한유전성대사질환학회지 Vol.5 No.1

Purpose: Rett syndrome (RTT) is an X-linked dominant neurodevelopmental disorder affecting 1 per 10,000~15,000 female births worldwide. The disease-causing gene has been identified as MECP2 (methyl-CpG-binding protein). In this study, we carried out diagnostic mutational analysis of MECP2 gene in RTT patients. Methods: We analyzed four exons and putative promoter of MECP2 gene from the peripheral blood of 43 Korean patients with RTT by PCR-RFLP and direct sequencing. Results: Mutations were detected in MECP2 gene about 60.5% of patients. The mutations consisted of 14 different types including 9 missense mutations, 4 nonsense mutations and 1 frameshift mutation. Of these, three mutations (G161E, T311M, P385fsX409) were newly identified and these were determined as disease-causing mutations by PCR-RFLP and direct sequencing analysis. Most of the mutations were located within MBD (42.3%) and TRD (50%). T158M, R270X, and R306C mutations were identified with high frequency. An intronic SNP (IVS3+23C>G) was newly identified in only three of the patients. It may be a disease-related and Korea-specific SNP with RTT. The L100V and A201V have been reported to be unclassified variant and SNP. However, these mutations were not found in more than 100 normal Korean control samples. These base substitutions seem to be the disease-causing mutations in Korean RTT contrary to previous studies. Conclusion: Disease-causing mutations and polymorphisms would be very important for diagnosing of RTT in Korean. The experimental procedure used in this study might be considered for molecular biologic diagnosis used in clinical field.

Local Distribution and Floral Preferences of Bumblebee Queens in Korea

Hyung Joo Yoon,Kyeong Yong Lee,Mi Ae Kim,In Gyun Park 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

Bumblebees are important pollinators of crops and wildflowers in northern temperate regions. The number of bumblebee species has declined in recent decades, particularly, in developing regions. Here, we investigated the distribution and floral preferences of founder bumblebee queens that were collected between 2000 and 2012 in Korea with the intention of gathering information useful in the conservation of native bumblebee populations. A total of 13,130 overwintered bumblebee queens were collected at 63 locations from 8 provinces; the collected bumblebees consisted of five species of native bumblebees (Bombus ardens, B. ignitus, B. hypocrita spporoensis, B. ussurensis and B. consobrinus wittenburgi) and one species of exotic bumblebee (B. terrestris). The dominant species were Bombus ardens Smith and Bombus ignitus Smith, which accounted for 7,462 (56.8%) and 3,725 (28.4%) of the collected bumblebees, respectively. As a proportional distribution of the founder bumblebee queens that were collected, B. ignitus was the most common (9.8%-78.5%), followed by B. ardens (9.8%-66.1%), B. hypocrita spporoensis (0.9%-31.4%), B. ussurensis (0.8%-22.2%), B. consobrinus wittenburgi (0.1%-3.8%) and B. terrestris (0.0%-1.1%). The bumblebee queens foraged from 7 families of plants and 18 plant species. B. ignitus bumblebees were primarily collected from Prunus serrulata Matsumura and Corydalis speciosa Max, and B. ardens bumblebees were primarily collected from Rhodldendron mucronulatum Turcz and Prunus yedoensis.