[논문]부산시 영도의 세 지역내 식물 집단 구조

허홍욱,이송진,최금자,허만규 부산대학교 환경문제연구소 2004 環境硏究報 Vol.22 No.-

Plant Population Structure of three areas in Young-do of Busan

사람의 혈관 내피세포에 대한 Rickettsia typhi의 감염

안욱수,이강순,송호연 순천향대학교 1994 논문집 Vol.17 No.4

R. typhi, the etiologic agent of murine typhus, has known to cause fever, headache, maculopapular rash and vasculitis of small blood vessel. The skin rash and vasculitis are thought to be the results of endothelial cell injury from the infection with R. typhi in vivo. R. typhi showed ability to cause cellular injury of cultured human endothelial cells. The endothelial cells used in this experiment wee obtained by collagenase treatment of human umbilical cord veins. These cells were conformed to be endothelial cell by the presence of von Willebrand factor through the immunofluorescent antibody technique and Weibel-Palade bodies under the electron microscopy. Ultrastructural study revealed that there were no evidences of entry and/or escape from endothelial cells of R. typhi in 24 hours through 48 hours after infection. On the contrary, numerous particles implicating R. typhi were observed in large vacuole within endothelial cells at 48 hours after inoculations. Estimating the severe injury of endothelial cells by infection of R. typhi, the endothelial cells seems to be the primary target cells in human disease caused by R. typhi infection.

자궁암세포(HeLa)에 대한 사군자탕과 수종 항암제의 병용투여 효과

은재순,김현욱,소준노,오찬호,이송재 又石大學校 1993 論文集 Vol.15 No.-

The studies were conducted to investigate the combined effects of Sa Kunja Tang(SKT) and several anti-cancer drugs. The effects of SKT and several anti-cancer drugs on the proliferation of HeLa cells, human cervical cell line, was estimated by MTT colorimetric assays. The SKT extract inhibited the proliferation of HeLa cell at 10^-4g/ml. The inhibitory action of the mitomycin C(MMC), mercaptopurine(MCP) and 5-fluorouracil(5-Fu) treated group, respectively, on the proliferation of HeLa cell was increased by the SKT. When the mice were treated by the MMC, the number of leukocyte was decreased significantly at the 1st and 3rd day, but recovered at the 7th day. In the groups of the MMC treated with the SKT, the number of leukocytes was increased significantly than the group of the MMC treated only at the 3rd day. The SKT extract decreased the thymus weight of mice. The SKT extract increased the number of plaque forming cells(PFC), but the MMC treated group decreased the number of PFC. The combined treatment of MMC and the SKT increased the number of PFC significantly than the MMC treated group. The SKT extract increased the proliferation of T cells, but the MMC treated group decreased the proliferation of T cells. The combined treatment of MMC and the SKT increased T cell proliferation significantly than the MMC treated group. In conclusion, the results presented in this paper suggest that the SKT extract can decrease the dose of anti-cancer drugs on HeLa cell, and recover the side dffects of the MMC, such as leukopenia and immunosuppresion, without any intercalating the anti-proliferative action of the MMC in vivo.

대학생들의 환경문제 의식에 관한 연구

허만규,이송진,허홍욱 한국환경과학회 2003 한국환경과학회지 Vol.12 No.3

This study was attempted to analyze sophomores' consciousness for environmental problems. In order to achieve this purpose, a method of questionnaire was administered to total 387 students selected from four universities in Busan. There were surveyed questions as follows, the conception of environmental problems, the condition of dwelling place and surrounding of home, the problems of environmental pollution, knowledge and concern for environmental problems, necessity of environmental education of school, and the environmental policy in Korea. Most students responded that the most serious environmental problem is water pollution. Most respondents have recognition the necessity of schooling for environmental education, whereas current-contents of environmental education are not enough to modify students' attitude and behavior.

Chlorpromazine이 백서 해마에서 전기경련 충격에 의한 MAPK 타이로신 인산화에 미치는 영향

주연호,정성훈,전송희,강웅구,김용식,김상욱,박주배 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.2

Object : In order to examine the interaction mechanisms of electroconvulsive shock(ECS) and antipsychotic drug at the level of molecular biology, we observed the effect of chlorpromazine pre-treatment on the activation of mitogen activated protein kinase(MAPK) induced by electroconvulsive shock(ECS) in rat hippocampus Method : Male Sprague-Dawley rats were divided into 2 groups. To the experimental group chlorpromazine(20㎎/㎏) was given intraperitoneally, and to the control distilled water was given instead. Thirty minutes later, ECS was given and the hippocampus was dissected out 2 minutes thereafter. Immunoblotting with antiphosphotyrosine antibody was carried out, and the signal intensity at 42kDa band was quantitized using densitometer. The obtained result was compared by student t-test between the experimental and the control group. The absolute amount of MAPK was measured by immunoblotting with anti-MAPK antibody. Result : The tyrosine phosphorylation of MAPK reached peak at 2 minutes after ECS. However, in the chlorpromazine pre-treated group, the peak level of MAPK tyrosine phosphorylation was significantly attenuated(t=-3.12, df=14, p=0.008) compared to the control. In contrast to this, the absolute amount of MAPK did not differ between the pretreated and the control group. Conclusion : Chlorpromazine attenuated the tyrosine phosphorylation of MAPK by ECS. This finding seems to be related to the fact that chlorpromazine pre-treatment changed the cfos expression by ECS in rat brain. Antipsychotic drug and ECS might interact at the level of MAPK signal transduction system, and this might explained the observed synergistic effect of two treatment modality.

Effects of Natural Zeolite on Mechanical and Thermal Properties of Epoxy Composite System

Song, Young Wook,Kim, Sang Wook 서울시립대학교 산업기술연구소 1996 산업기술연구소논문집 Vol.4 No.-

The natural zeolite was introduced to diglycidyl ether of bisphenol A(DGEBA)/4,4'-methylene dianiline(MDA)/hydroquinone-phenyl glycidyl ether (HQ-PGE) system to modify mechanical and thermal properties of epoxy resin as an inorganic filler. In mechanical properties, the tensile strength of a system with 10 phr of natural zeolite decreased about 17 % than that of a system without natural zeolite. Also, the impact strength of a system with the natural zeolite decreased than that of the system without natural zeolite. Both properties decreased with the increment of natural zeolite contents. In case of thermal properties, glass transition temperature(Tg) and decomposition temperature(Td) of the composites system were seldom affected by natural zeolite and its contents.

Colonic Pseudo-obstruction With Transition Zone: A Peculiar Eastern Severe Dysmotility

Eun Mi Song,Jong Wook Kim,Sun-Ho Lee,Kiju Chang,Sung Wook Hwang,Sang Hyoung Park,Dong-Hoon Yang,Kee Wook Jung,Byong Duk Ye,Jeong-Sik Byeon,Suk-Kyun Yang,Hyo Jeong Lee,Chang Sik Yu,Chan Wook Kim,Seong 대한소화기 기능성질환∙운동학회 2019 Journal of Neurogastroenterology and Motility (JNM Vol.25 No.1

Background/Aims Previous studies from Korea have described chronic intestinal pseudo-obstruction (CIPO) patients with transition zone (TZ) in the colon. In this study, we evaluated the pathological characteristics and their association with long-term outcomes in Korean colonic pseudo-obstruction (CPO) patients with TZ. Methods We enrolled 39 CPO patients who were refractory to medical treatment and underwent colectomy between November 1989 and April 2016 (median age at symptoms onset: 45 [interquartile range, 29–57] years, males 46.2%). The TZ was defined as a colonic segment connecting a proximally dilated and distally non-dilated segment. Detailed pathologic analysis was performed. Results Among the 39 patients, 37 (94.9%) presented with TZ and 2 (5.1%) showed no definitive TZ. Median ganglion cell density in the TZ adjusted for the colonic circumference was significantly decreased compared to that in proximal dilated and distal non-dilated segments in TZ (+) patients (9.2 vs 254.3 and 150.5, P < 0.001). Among the TZ (+) patients, 6 showed additional pathologic findings including eosinophilic ganglionitis (n = 2), ulcers with combined cytomegalovirus infection (n = 2), diffuse ischemic changes (n = 1), and heterotropic myenteric plexus (n = 1). During follow-up (median, 61 months), 32 (82.1%) TZ (+) patients recovered without symptom recurrence after surgery. The presence of pathological features other than hypoganglionosis was an independent predictor of symptom recurrence after surgery (P = 0.046). Conclusions Hypoganglionosis can be identified in the TZ of most Korean CPO patients. Detection of other pathological features in addition to TZ-associated hypoganglionosis was associated with poor post-operative outcomes.

A Combinational Therapy of Articular Cartilage Defects: Rapid and Effective Regeneration by Using Low-Intensity Focused Ultrasound After Adipose Tissue-Derived Stem Cell Transplantation

Song Byeong-Wook,Park Jun-Hee,Kim Bomi,Lee Seahyoung,Lim Soyeon,Kim Sang Woo,Choi Jung-Won,Lee Jiyun,Kang Misun,Hwang Ki-Chul,Chae Dong-Sik,Kim Il-Kwon 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.3

BACKGROUND: Although low-intensity pulsed ultrasound has been reported to be potential cartilage regeneration, there still unresolved treatment due to cartilage fibrosis and degeneration by a lack of rapid and high-efficiency treatment. The purpose of this study was to investigate the effect of a combination therapy of focused acoustic force and stem cells at site for fast and efficient healing on cartilage regeneration. METHODS: Using a rat articular cartilage defects model, one million adipose tissue-derived stem cells (ASCs) were injected into the defect site, and low-intensity focused ultrasound (LOFUS) in the range of 100–600 mV was used for 20 min/day for 2 weeks. All experimental groups were sacrificed after 4 weeks in total. The gross appearance score and hematoxylin and eosin (H&E), Alcian blue, and Safranin O staining were used for measuring the chondrogenic potential. The cartilage characteristics were observed, and type II collagen, Sox 9, aggrecan, and type X collagen were stained with immunofluorescence. The results of the comprehensive analysis were calculated using the Mankin scoring method. RESULTS: The gross appearance scores of regenerated cartilage and chondrocyte-like cells in H&E images were higher in LOFUS-treated groups compared to those in negative control or ASC-treated groups. Safranin O and Alcian blue staining demonstrated that the 100 and 300 mV LOFUS groups showed greater synthesis of glycosaminoglycan and proteoglycan. The ASC ? LOFUS 300 mV group showed positive regulation of type II collagen, Sox 9 and aggrecan and negative regulation of type X collagen, which indicated the occurrence of cartilage regeneration based on the Mankin score result. CONCLUSION: The combination therapy, which involved treatment with ASC and 300 mV LOFUS, quickly and effectively reduced articular cartilage defects.

Life Prediction of High Pressure Steam Turbine Rotor

Song, Gee Wook,Kim, Bum Shin,Hyun, Jung Seob Trans Tech Publications, Ltd. 2006 Key Engineering Materials Vol.321 No.-

<P>The operation mode of thermal power plants has been changed from the base load to duty cycle. From the changeover, fossil power plants cannot avoid frequent thermal transient state, for example, start up and stop, which results in thermal fatigue damage at the heavy section components. The rotor is the highest capital cost component in steam turbine and requires long outage for replacing the new one. For optimized power plant operation life and inspection management of rotor is necessary. It is known as general that start-up and shutdown operation greatly affect on steam turbine life. The start-up operation condition is especially severe because of the rapid temperature and rotational speed increase, which causes damage and reduction of main components life of steam turbine. The start-up stress of rotor which is directly related life is composed of thermal and rotational stresses. The thermal stress is due to the variation of steam flow temperature and rotational stress is due to the rotation speed of itself. In this paper, the analysis method of start-up stress of rotor which considers simultaneously temperature and rotation speed transition is proposed, which includes a case study regarding 500MW fossil power plant steam turbine rotor. Also, the method of damage quantitative estimation of fatigue damage to operation condition is described. The method can be applied to find weak points to the fatigue damage. Using the method, total life consumption can be obtained, and can be also used for determining future operation mode and the life extension of old fossil power units.</P>

Correlation of doping concentration, charge transport of host, and lifetime of thermally activated delayed fluorescent devices

Song, Wook,Lee, Woochul,Kim, Kong Kyeom,Lee, Jun Yeob Elsevier 2016 ORGANIC ELECTRONICS Vol.37 No.-

<P><B>Abstract</B></P> <P>Origin of doping concentration dependence of lifetime of thermally activated delayed fluorescent (TADF) devices was examined using a TADF emitter doped in a hole transport type and a bipolar host material. Lifetime of the hole transport type host based TADF device was increased according to doping concentration of TADF emitter, while that of the bipolar host based TADF device was decreased according to doping concentration of TADF emitter. The doping concentration dependence of the lifetime could be correlated with recombination zone of the emitting layer. Broad recombination zone at high doping concentration in the hole transport type host and at low doping concentration in the bipolar host was proposed as the main contributor of the doping concentration dependence of the lifetime.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lifetime increase in thermally activated delayed fluorescent devices with a unipolar host according to doping concentration. </LI> <LI> Constant lifetime in thermally activated delayed fluorescent device with a bipolar host according to doping concentration. </LI> <LI> Correlation of recombination zone broadening with lifetime of thermally activated delayed fluorescent devices. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>