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Sung Woo Kim,Min Soo Kim,Yeonhui Yu,Chan-Lan Kim,Ik Soo Jeon,Chongdae Kim 한국동물생명공학회(구 한국동물번식학회) 2017 Reproductive & Developmental Biology(Supplement) Vol.41 No.2
This study examined factors affecting the analysis of motility of chicken semen. The viability of spermatozoa was estimated using varying dilution ratios and supplementation with BSA or fatty acid free (FAF)-BSA as protein sources in semen diluent. Fresh semen was examined after preparing dilutions in BPSE of 1/8, 1/16 and 1/32 at 25℃. The motility of incubated semen at each dilution was observed at 3 min (89.9%, 69.9 % and 53.2%), 30 min (86.7%, 71.4% and 51.7%), 1 h (89.5%, 74.0% and 53.5%) and 3 h (78.5%, 66.5% and 45.7%), respectively. The addition of BSA or FAF-BSA to BPSE diluent significantly increased the viability of semen in 1/32 dilution with results of 53.2% (control), 84.8% (BSA) and 92.9% (FAF-BSA) (p<0.05). This phenomenon was also observed in the dilution of frozen semen, where FAF-BSA treatment increased the viability of thawed semen from 17.6% to 34.0% in a 1/8 dilution (p<0.05). When the protein sources were used in the dilution, the survival rates of diluted chicken semen were also increased with time lapse. These results show that FAF-BSA may act to protect chicken semen and is suitable as a basic component of chicken semen diluent for the method of analyzing rooster semen after freezing.
Jeon, Seung-Bae,Kim, Weon-Guk,Park, Sang-Jae,Tcho, Il-Woong,Jin, Ik-Kyeong,Han, Joon-Kyu,Kim, Daewon,Choi, Yang-Kyu Elsevier 2019 Nano energy Vol.65 No.-
<P><B>Abstract</B></P> <P>In the internet of things (IoT) era, electronic textile (E-textile), which combines diverse functional parts on a fabric substrate, has attracted much attention. Among the various components for E-textile, the human-machine interface device is one of the most important. Here, a wearable fabric touchpad based on a triboelectric generator (TEG) is demonstrated. It is created only with inexpensive commercialized fabrics. Long string-shaped fabric TEGs act as the rows and columns of a crossline array, and each cross-point of a unit cell at each row and column constructs a unit pixel. The proposed touchpad accurately traces basic motions such as the vertical tapping and lateral dragging of a polytetrafluoroethylene (PTFE) stylus and generates corresponding output signals. Based on this capability, the proposed touchpad can be utilized for the handwriting of digits and for their recognition as well. Digits from 0 to 9 written on the touchpad are successfully classified by a pre-trained neural network with very high accuracy of 98%. This implies that the proposed wearable touchpad has immediate practicality. Considering its cost-effectiveness, compatibility with the textile industry, and its accurate operation, the proposed touchpad is a strong candidate for use in interface devices in the future E-textile industry.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A self-powered wearable touchpad was demonstrated using a fabric-based TEG array. </LI> <LI> Only cheap commercialized fabrics were employed for the general usage. </LI> <LI> Basic touchpad operation was experimentally verified. </LI> <LI> A digit recognition program was integrated to the proposed touchpad. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
The Manufacturing Method of Open Pulled Straw (OPS) using Digital Heating Gun
Sung Woo Kim,Min-Su Kim,Chan-Lan Kim,Ik Soo Jeon 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10
Vitrification is a common technique of cryopreservation in animal genetic resources of oocytes or embryos and replacing slow freezing methods. This study examined the factors of preparing open pulled straw (OPU) that could be used for vitrification with digital heating gun (DHG). Thermal performance of heat transfer of DHG is more stable and adjustable than traditional way of heating method like hot-plate or alcohol lamp. Thermal setting value of DHG was adjusted to 250, 350, 400 and 450℃ and production rate of wanted OPS was examined. The thickness of wall and outer diameter of OPU were measured by microscopy. OSP production efficiency of 0.5 mL straw was observed at 0, 66.1, 90.5 and 85.7% for 1~2 sec and 9.5, 33.3, 47.6 and 23.8% for 2~3 sec of heating time. OPS with inner diameter in the range of 200~650 μm was produced with 0.5 mL straw, and OPS with inner diameter in the range of 150~300 μm was obtained with 0.25 mL straw. OPS at the desired length and size could be obtained easily when straw was heated for 1.6~2.3 seconds at 350~400℃. Production rates of 0.25 mL straw were higher than those of 0.5 mL straw, so it was needed more attention to control time and power that were required for optimal heat transfer with 0.5 mL straw. Based on the data obtained in this study, hand-made OPSs could be produced for vitrification devices to freeze COCs, oocytes and embryos of with various size of outer diameter.
The Survival Rate of Epididymal Sperm from Korean Native Black Goat in 0.25 mL Straw
Sung Woo Kim,Jinwook Lee,Chan-Lan Kim,Kwan Woo Kim,Yeong Gyu Ko,Ik Soo Jeon 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06
This study examined factors affecting survival rate of epididymal sperm from Korean native black goat using 0.25 mL straw. The sperm was cryopreserved by the height above 5 cm or 14 cm from the surface of liquid nitrogen (LN2) for 10 min and then plunged into the LN2. The motility of sperm after thawing was estimated using CASA system and movement analysis. The motile sperm rates after thawing at 14 cm freezing height was significantly higher than at 5 cm (85.3% vs. 73.0%). Also the hyper- activation rates showed that the more actively moving sperm counts appeared at 14 cm height experiment (55.3% vs. 47.6%). This phenomenon was also observed in the dilution of electro-ejaculted semen. So, these results show that testis originated sperm could be used for artificial insemination and fertility evaluation in vivo is needed to evaluate their contribution to animal genetic resource banks.