http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Jin, Hyunjung,Do, Jihye,Moon, Dahyun,Noh, Eun Woon,Kim, Wook,Kwon, Mi Springer-Verlag [etc.] 2011 Planta Vol.234 No.5
<P>A cDNA library was constructed from secondary xylem in the stem of a 2-year-old yellow poplar after being bent for 6?h with a 45 configuration to isolate genes related to cell wall modification during the early stages of tension wood formation. A total of 6,141 ESTs were sequenced to generate a database of 5,982 high-quality expressed sequence tags (ESTs). These sequences were clustered into 1,733 unigenes, including 822 contigs and 911 singletons. Homologs of the genes regulate many aspects of secondary xylem development, including those for primary and secondary metabolism, plant growth hormones, transcription factors, cell wall biosynthesis and modification, and stress responses. Although there were only 1,733 annotated ESTs (28.9%), the annotated ESTs obtained in this study provided sequences for a broad array of transcripts expressed in the stem upon mechanical bending, and the majority of them were the first representatives of their respective gene families in Liriodendron tulipifera. In the case of lignin, xylem-specific COMTs were identified and their expressions were significantly downregulated in the tension wood-forming tissues. Additionally, the majority of the auxin- and BR-related genes were downregulated significantly in response to mechanical bending treatment. Despite the small number of ESTs sequenced in this study, many genes that are relevant to cell wall biosynthesis and modification have been isolated. Expression analysis of selected genes allow us to identify the regulatory genes that may perform essential functions during the early stages of tension wood formation and associated cell wall modification.</P>
HyunJung LEE,JiHo YANG,Hyun Jin JO,Jung Hwan OH,Fatih KARADENIZ,Youngwan SEO,Chang-Suk KONG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The halophyte Artemisia scoparia has been used as a folk medicine for the treatment of inflammation, urethritis and hemorrhage. Recent studies have shown that A. scoparia had physiological activities such as antioxidant, anti-inflammatory and anti-obesity. In this study, the effect of A. scoparia on photoaging in HDF cells was investigated by using its crude extract and solvent fractions (n-hexane, 85% aq. MeOH, n-BuOH and H₂O). Based on the results of this study, A. scoparia was suggested as a potential source for functional raw materials for the cosmetic industry. Furthermore, the studies that can verify the active ingredients of A. scoparia will contribute immensely to the research and development of cosmeceuticals from halophytes. Also, future studies using A. scoparia as a cosmetic material source are expected to produce high value-added resources unknown to applied dermatology field and strengthen the competitiveness of the domestic cosmetics industry by while promoting the utilization of undervalued coastal wetland plants.
Jin, Tae-Eun,Jang, Miae,Kim, Hyunjung,Choi, Yu Mi,Cho, Hana,Chung, Sungkwon,Park, Myoung Kyu Korean Society for Molecular Biology 2008 Molecules and cells Vol.26 No.3
<P>Corticotropin releasing factor (CRF) mediates various responses to stress through CRF receptors 1 and 2. CRF receptor 2 has two forms, 2alpha and 2beta each of which appears to have distinct roles. Here we used dopaminergic neuron-derived MN9D cells to investigate the function of CRF receptor 2 in dopamine neurons. We found that n-butyrate, a histone deacetylase inhibitor, induced MN9D cell differentiation and increased gene expression of all CRF receptors. CRF receptor 2beta was minimally expressed in MN9D cells; however, its expression dramatically increased during differentiation. CRF receptor 2beta expression levels appeared to correlate with neurite outgrowth, suggesting CRF receptor 2beta involvement in neuronal differentiation. To validate this statement, we made a CRF receptor 2beta-overexpressing MN9D/CRFR2 beta stable cell line. This cell line showed robust neurite outgrowth and GAP43 overexpression, together with MEK and ERK activation, suggesting MN9D cell neuronal differentiation. From these results, we conclude that CRF receptor 2beta plays an important role in MN9D cell differentiation by activating the MEK/ERK signaling pathway.</P>