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Hyung Keun Oh,Hyung Joo Yoon,Min Jee Kim,Xinlong Wan,Iksoo Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
The bumblebee, Bombus ignitus (Hymenoptera: Apidae), is a valuable natural resource that is one of the most notably utilized for greenhouse pollination in Korea. In order to understand the nature of genetic relationships, gene flow, and population structure of the species we sequenced a partial COI gene of mitochondrial DNA (mtDNA) corresponding to “animal barcode” region and the complete internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA (nrDNA) with the individuals collected from Korean localities. Although the 658-bp long mtDNA sequence provided only six haplotypes with the maximum sequence divergence of 0.456% (3 bp, from 91 individuals), but the ITS sequences provided 33 sequence types with the maximum sequence divergence of 0.78% (16 bp, from 35 individuals), confirming better applicability of the ITS sequences to the study of intraspecific variation. The ITS2 sequences of B. ignitus were shown to be the longest among known insects, ranging in size from 2,034 bp ~ 2,045 bp and harbored two duplicated repeats, indicating unusual structure of B. ignitus ITS2 sequences compared with other insect ITS sequences. Overall, a very high per generation migration ratio, a very low level of genetic fixation, and no discernable hierarchical population/population group were noted to exist among populations of B. ignitus on the basis of both molecules, thus suggesting that the B. ignitus populations on the Korean peninsula are panmictic, which is consistent with our understanding of the dispersal capability.
Hyung Keun Oh,Hyung Joo Yoon,Min Jee Kim,Xinlong Wan,Seong Ryul Kim,Yeon Soo Han,Iksoo Kim 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
The bumblebee, Bombus ignitus (Hymenoptera: Apidae), is a valuable natural resource that is one of the most notably utilized for greenhouse pollination in Korea. In order to understand the nature of genetic relationships, gene flow, and population structure of the species we sequenced a partial COI gene of mitochondrial DNA (mtDNA) corresponding to “animal barcode” region and the complete internal transcribed spacer 2 (ITS2) of the nuclear ribosomal DNA (nrDNA) collected from Korean localities. Although the 658-bp long mtDNA sequence provided only six haplotypes with the maximum sequence divergence of 0.61% (4 bp), the ITS sequences provided 84 sequence types with the maximum sequence divergence of 1.02% (21 sites), confirming better applicability of the ITS sequences to the study of intraspecific variation. The complete ITS2 sequences of B. ignitus were shown to be longest among known insects, ranging in size from 2,034 bp ~ 2,052 bp, harboring two duplicated repeats. Overall, a very high per generation migration ratio, a very low level of genetic fixation, and no discernable hierarchical population/ population group were noted to exist among populations of B. ignitus on the basis of both molecules, thus suggesting that the B. ignitus populations on the Korean peninsula are panmictic, which is consistent with our understanding of the dispersal capability of the species
Budiasih W. Solihin,Mi Hwan Kim,Byung Soon Im,Jae Yoon Cha,Gi Hyung Ryu 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.3
The objective of this experiment was to study the influence of feed moisture content on the degree of enzymatic hydrolysis of corn starch in a twin screw extruder and the saccarification yield of the dried extrudate. The feed moisture content was set at 25, 30, and 35% and α-amylase solution was directly injected into the feed section at a barrel temperature of 95℃ and screw speed of 250 rpm. Amyloglucosidase was used for the saccharification of the dried extrudate at a concentration of 0.055%(w/w). Expansion ratio and swelling factor of extrudates decreased with increasing the feed moisture content. Addition of α-amylase during extrusion process raised reducing sugar content of extrudates which also increased with the feed moisture content. The saccharification yield of dried extrudate was higher for the extrudate with lower feed moisture content.
김운형(Kim, Woon-Hyung),김종성(Kim, Jong-Sung),Bukowski, Richard W.,최병배(Choi, Byung-Bae) 한국화재소방학회 2012 한국화재소방학회 학술대회 논문집 Vol.2012 No.춘계
비상시 엘리베이터의 피난 활용은 피난층과 함께 초고층 및 복합연계건축물의 새로운 피난 수단으로 인식되며 현재 많은 연구가 진행되고 있다. 그러나 현 상황에서 엘리베이터의 고려는 대부분 설계단계에서 이루어지나 비상대응계획 차원에서의 명확한 가이드라인 없이 설계자의 판단에 의하여 진행된다. 이를 위하여 본 연구에서는 기존 연구 문헌과 설계자들에 의하여 현재 고려되어지는 요소를 인적, 환경적, 전략적인 고려요소로 구분하여 검토하였다. 엘리베이터 피난에 고려되는 요소를 목적과 기능, 역할에 따라 하위 단계위 구성요소를 설정하였으며 각각의 요소는 상위 요인에 종속되는 것으로 설계하였다. 종합적으로 볼 때 엘리베이터 피난에 있어서의 많은 요소가 고려되어지며, 각각의 요소는 계단과 피난층 등의 피난수단의 복합적인 이용에 따른 피난설계단계뿐만 아니라 유지관리단계에 있어서도 영향을 끼치는 것으로 분석되었다.