완두 F_1세대의 조합능력 검정

정원복,오주성,황필성,황영현,신두철,한원영,김수경 동아대학교 교육대학원 2002 동아교육논총 Vol.28 No.-

완두의 이면교잡에 의한 F_1세대에 대한 조합능력을 검정한 결과는 다음과 같다. 9개 형질에 대한 GCA, SCA, RCA효과를 검정한 바 조합능력은 전 형질에서 GCA, SCA, RCA가 모두 유의하였고, 분지수·경직경·엽장·주당협수·협당립수·주당립수·100립중·주당립중은 GCA가 SCA보다 크고, 품종간 GCA효과는 고성종1호가 경직경과 100립중에서, 고성종2호가 경장·분지수·엽장에서, 김해종이 주당협수·주당립수·100립중·주당입중에서, 진해종이 협다립수에서 각각 정(+)으로 높았다. SCA효과에서 경장은 1×2(고성종1호×고성종2호)조합과 1×3(고성종1호×김해종)조합이, 분지수는 4×5(고성종3호×진해종)조합이, 경직경과 엽장은 1×3(고성종1호×김해종)조합이, 주당협수는 1×2(고성종1호×고성종2호)조합과 4×5(고성종3호×진해종)조합이, 협당립수는 1×2(고성종1호×고성종2호)조합·1×4(고성종1호×고성종3호)조합·2×3(고성종2호×김해종)조합이, 주당립수는 1×2(고성종1호×고성종2호)조합과 4×5(고성종3호×진해종)조합이, 100립중은 3×4(김해종×고성종3호)조합이, 주당립중은 1×2(고성종1호×고성종2호)조합·3×4(김해종×고성종3호)조합·4×5(고성종3호×진해종)조합이 각각 정으로 높았다. RCA효과에서 경장·엽장·주당협수·주당립중은 1×2(고성종1호×고성종2호)조합이, 주당협수·주당립수·100립중·주당립중은 2×4(고성종2호×고성종3호)조합이 각각 정으로 높았다. Five pea varieties in F₁ generation of the possible crosses among them were used to estimate the combining ability for nine characters, i. e., stem length, number of branches per plant, stem diameter, leaf length, number of pods per plant, number of seeds per pod, number of seeds per plant, 100-seed weight and seed weight per plant. Data for F₁ hybrid and parents were subjected to the analysis method proposed by Griffing. The results obtained were summarized as follows. Mean squares of general combining ability(GCA), specific combining ability(SCA) and reciprocal combining ability(RCA) were significant for all characters observed. Mean squares of GCA were higher than those of SCA and RCA in number of branches per plant, stem diameter, leaf length, number of pods per plant, number of seeds per pod, number of seeds per plant, 100-seed weight and seed weight and seed weight per plant. Variety Gimbae showed the highest GCA effect for number of pods per plant, number of seeds per plant and seed weight per plant and positively high effects of GCA were observed from variety Gosung#1 in stem diameter, 100-seed weight, and from variety Gosung#2 stem length, number of branches per plant, leaf length, from Jinhae in number of seeds per pod In SCA effects, hybrids in Gosung#1×Gosung#2 were wxhibited positively high for stem length, number of pods per plant, number of seeds per pod, number of seeds per plant and seed weight per plant, and high SCA effects for number of branches per plant, number of pods per plant, number of seeds per plant and seed weight per plant were found positively in Gosung#3×jinhae. In RCA effects, hybrids in Gosung#2×Gosung#3 were exhibited positively high for number of pods per plant, number of seeds per plant, 100-seed weight and seed weight per plant and high RCA effects for stem length, leaf length, number of pods per plant, and seed weight per plant were found positively in Gosung#1×Gosung#2.

뇨(尿)중 3-Methylhistidine 함량의 측정과 이용

정수현,서형주,김윤숙,이효구,강덕호 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.2

기존의 방법을 일부 수정하여 뇨중 3-methylhistidine을 분석하였다. 뇨중 3-methylhistidine을 fluorescamine 유도체화하여 HPLC에 주입하고 C_18 column과 10 mM acetonitrile/sodium phosphate buffer(pH 7.5)로 분리·용출시켜 형광검출기로 측정하였다. 3-methylhistidine의 체류시간은 7분 이내이었으며, histidine과의 분리상태도 서로 간섭함이 없이 양호하였다. 뇨에 3-methylhistidine을 첨가하고 이를 분석하였을 때의 회수율은 93∼106%로 높은 수준이었다. 체육학과 남학생중 웨이트 트레이닝 단련자와 비단련자를 대상으로 조사한 단기간의 웨이트 트레이닝에 따른 뇨중 3-methylhistidine 함량의 변화는 두 집단 모두 웨이트 트레이닝후의 3-methylhistidine 분비량이 유의하게 증가하였다. A modified method is given for the precolumn derivatization and subsequent high-pressure liquid chromatographic seperation of 3-methylhistidine from urine. The elution contained isocratic solution with acetonirile and 10 mM sodium phosphate(pH 7.5) requires less than 7 min. The recoveries of 3-methylhistidine from urine control were 93% to 106%. 3-Methylhistidine determinations were performed on urine samples from volunteers who were both male trained and non-trained physical undergraduates. As the result, urinary 3-methylhistidine content of volunteers increased significantly after weight training.

흰쥐 편도체에서 칼슘결합단백인 calbindin-D28K함유 신경세포의 생후 발달에 관한 면역조직학적 연구

정윤영,김종중,문정석,박영란,정상봉,김현곤,박상수 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Background and Objectives : The calcium-binding protein, calbindin-D28K(CB) is widely distribution of CB in rat amygdala during postnatal maturation, in order to provide an insight into the developmental role it may play in the amygdaloid neurons. Materials and Methods : The present study is based on 28 postnatal cases ranging from the day of birth, postnatal day 0 (P0) to 30 days (P5, P10, P15, P20, P30), and these cases were compared with adult rat amygdala. Cryostat sections processed free-floating with monoclonal antibody by immunohistochemistry. Results : CB-immunoreactive neurons were already found at birth in all amygdaloid nuclei except the intercalated nucleus. The intensity and number of immunoreactivity of CB-containing neurons increased during the first 10 days of postnatal life but dramatically decreased at P15. Mature patterns of CB-immunoreactive neurons were achieved at P20. In the adult amygdala, CB-immunoreactive neurons were distributed in almost all amygdaloid nuclei except the intercalated nucleus. Conclusion : The present results show early appearance of CB immunoreactivity in the amygdala. It may be related to the early appearance of CB immunoreactivity in many other brain regions, and suggests that CB may play a functional role during the development of the nervous system including amygdala.

파킨슨씨병 환자에 있어서 자가부신수질 이식술

정상섭,박상근,오성훈,김선호,윤도흠,박정수,양우익,최인준,안영수,김경환,박창일,김진수,이현철 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.6

Recent experimental studies and clinical cases have suggested that grafting tissue from the adrenal medulla into the brain may alleviate the symptoms of Parkinson's disease. Autologous adrenal medullary grafts into the striatum was performed stereotactically in two patients with Parkinson's disease. Both patients were bedridden preoperatively, but 4 months after the surgery both became ambulatory and one was even capable of returning to his job. Even though the number of cases was small⑵ and the follow-up period was short, this study shows that adrenal medullary transplantation into the striatum may have some beneficial effects in patient with Parkinson's disease. Further experimental and clinical trials are necessary to see whether this procedure will be a recommendable surgical treatment for patients suffering from Parkinson's disease.

외상성 간손상과 비장손상시 CT분류의 의의

정준영,이창현,김현정,도호석,이삼범,도병수 大韓應急醫學會 1997 대한응급의학회지 Vol.8 No.1

The Organ Injury Scaling Committee of the American Association for the Surgery of Trauma recently published a consensus classification of hepatic and splenic injuries. The hepatic and splenic injury scales, based on parenchymal laceration and intrahepatic hematoma, include grade 1 to 6 and grade 1 to 5 respectively, representing the least to most severe injury. Forty-eight patients with hepatic and splenic injury were evaluate by abdominal CT from January 1995 to May 1996 and we compared abdominal CT grading with operative grading. Four CT grades did not correlate with operative finding. CT grading showed a sensitivity of 80%, specificity 80% in regard to operative grading. We conclude that 1) CT is an accurate technique to determine the extent of hepatic and splenic injury. 2) CT grading of hepatic and splenic trauma has a high correlation with operative grading.

불안정 대퇴골 전자간 골절 치료에서MHS (Intramedullary Hip Screw)와 압박고 나사의 비교

정필현,황정수,강석,김종필,곽주영 대한고관절학회 2004 Hip and Pelvis Vol.16 No.1

2인과 3인의 기본 심페소생술 비교

정준영,이창현,김현정,도호석,이삼범,도병수 大韓應急醫學會 1997 대한응급의학회지 Vol.8 No.1

Study objectives: To compare the quality of CPR provided by medical students and pre-EMTs perfoming three-rescuer CPR with that achieved by same students trained to provide standard two-rescuer CPR and to find the most effective method of resuscitation and to educate this methods to students. Material and methods: From July 1 to September 30, 1996, we had trained 60 groups consisted of the medical students, students of EMS technology and pre-EMT. Each group practiced two-rescuer CPR and three-rescuer CPR for 120 minutes with Resusci? Anne and perfomed CPR for at least 3 minutes without feedback. To eliminate any visual cues of perfomance the mannequin's recorder was hidden. Each actions were recorded by Resusci? skillmeter and Laerdal printer. To compare the quality of two-rescuer CPR and three-rescuer CPR, we analyzed the data by SPSS with paired t-test. Results: Three-rescuer teams delivered a mean minute ventilation and a mean minute cardiac compression substantially greater than that produced by two-rescuer teams(11.30±1.85 vs 9.09±2.22, p<0.001; 99.22±12.42 vs 95.12±15.75, p<0.001). Three-rescuer teams produce the more correct actions in chest compression and ventilation(93.84±7.56% vs 83.49±16.94%, 90.90±9.33% vs 77.00±17.06%). Conclusion: Three-rescuers can produce better CPR than two rescuers when a bag-valve-mask devices is used and the technique is easily learned and readily retrained, so we think that repeated education and training of this CPR methods to students is needed.

맥주맥 F_1세대의 조합능력 검정

정원복,오주성,황필성,김수동,서세정,현종내,김대호 東亞大學校 2002 東亞論叢 Vol.39 No.-

맥주맥의 이면교잡에 의한 F_1세대에 대한 조합능력을 검정한 결과를 요약하면 다음과 같다. 8개 형질에 대한 GCA, SCA, RCA효과를 검정한 바 조합능력은 전 형질에서 GCA, SCA, RCA가 모두 유의하였는데 간장, 수장, 망장, 간직경, 곡핍폭, 1수립수 1000립중은 GCA가 SCA보다 크고, 품종간 GCA효과는 진양보리가 간장에서 부(-)이고, 삼도보리가 수장·망장·간직경·1000립중에서, 사천6호가 망장과 간직경에서, 진광보리가 곡립장에서, 두산29호가 곡립폭·1수립수·1000립중에서 각각 정(+)으로 높았다. SCA효과에서 수장은 두산29호×두산8호 조합이, 망장은 진양보리×두산29호 조합이, 간직경은 사천6호×진광보리 조합이, 곡립장은 진양보리×두산8호 조합이, 곡립폭은 진광보리×두산8호 조합이, 1수립수는 진양보리×삼도보리 조합이, 1000립중은 사천6호×두산8호 조합 및 두산29호×두산8호 조합이 각각 정으로 높았고, 간장은 삼도보리×진광보리 조합이 부로서 높았다. RCA효과에서 수장은 두산8호×남향보리 조합이, 망장은 진양보리×삼도보리 조합이, 간직겨은 사천6호×진양보리 조합이, 곡립장은 사천6호×진광보리 조합이, 곡립폭은 진양보리×삼도보리 조합이, 1수립수는 사천6호×두산29호 조합이, 1000립중은 진양보리×두산29호 조합이 각각 정으로 높았고, 간장은 사천6호×두산29호 조합이 부로서 높았다. Seven barley varieties in F_1 generation of the possible crosses among them were used to estimate the combining ability for eight characters, i.e., culm length, spike length, awn length, culm diameter, grain length, grain width, number of grains per spike and 1000 grains weight. Data for F_1 hybrid and parents were subjected to the analysis method proposed by Griffing. The results obtained were summarized as follows. mean squares of general combining ability(GCA), specific combining ability(SCA) and reciprocal combining ability(RCA) were significant for all characters observed. Mean squares of GCA were higher than those of SCA and RCA in culm length, spike length, awn length, culm diameter, grain width, number of grains per spike and 1000 grains weight. Variety Dusan#29 showed the highest GCA effect for number of grains per spike and positively high effects of GCA were observed from variety Samdo bori in spike length, awn length, culm diameter, 1000 grains weight, and from variety Jinkwang bori I grain length, from Dusan#29 in grain width, umber of grains per spike, 1000 grains weight. Also Jinyang bori showed negatively high GCA effect in culm length. In SCA effects, hybrids in Jinyang bori × Samdo bori were exhibited positively high for number of grains per spike and high SCA effects for 1000 grins weight were found positively in Sacheun#6×Dusan#8 and Dusan#29×Dusan#. In RCA effects, hybrids in Sacheun#6×Dusan#29 were exhibited positively high for number of grains per spike and high RCA effects for 1000 grains weight were found positively in Jinyang bori×Dusan#29.

영지버섯(Ganoderma Iucidum) 생장점으로부터 분리한 단백다당류의 면역활성

정경수,김상범,정수현 충남대학교 약학대학 의약품개발연구소 1997 藥學論文集 Vol.13 No.-

Two protein-polysaccharide fractions. GLA and GLB, respectively, were prepared from the pileus of the fully grown carpophores and the tips of the growing carpophores of Ganoderma lucidum. At a dose of 100 ㎎/㎏/day ip. GLA and GLB inhibited the growth of sarcoma 180 solid tumor in ICR mice by 56.3% and 81.8%, respectively. In a flow cytometric (FCM) analysis. GLA and GLB enhanced the formation of lymphoblasts of BALB.c splenic leukocytes at a concentration of 100㎍/㎖, by 38.3% and 61.3%, respectively. When ip injected into ICR mice. GLB exerted anti-leukopernic effect against cyclopospamide (75 ㎎/㎏/day ip) in that the leukocyte counts of the peripheral blood of the normal and the cycloposphamide-treated mice, respectively, was (11.1±3.8)×10 exp(3) and (4.0±1.8)×10 exp(3), while the GLB-cyclophosphamide treated mice showed a leukocyte count of (10.8±5.1)×10 exp(3) cells/㎕. These results suggest that GLB is a promising candidate for an effective cancer immunotherapeutic agent.

비확공성 골수강내 금속정을 이용한 폐쇄성 경골 간부 골절의 치료

정필현,황정수,채동주,문상호 東國大學校 1998 東國論叢 Vol.37 No.-

Most of closed fractures of the tibial shaft can be treated successfully with medullary nails. Reamed intramedullary nailing technique has become a good alternative method in treating the close tibial fracture. Unreamed interlocking intramedullary nailing has been employed successfully in the treatment of open tibial fractures. This study was undertaken to evaluate the result of interlocking intramedullary nailing without reaming in the treatment of closed fractures of the tibial shaft. From 1995 through 1998, fifty four cases of the closed, displace tibial fractures were treated by an unreamed intramedullary nailing and the average follow-up period was 1 year and 5 months(ranged from 12 months to 27 months). The results were as follows : 1. Fifty four cases were treated with closed, unreamed nailing technique and the nail which has 1mm smaller diameter than isthmic width of unaffected tibia was choosen. 2. The average time to bony union was achieved at 17 weeks without abundant periosteal b callus formation. 3. Eight tibiae were required the additional procedure such as autogenous bone graft, skin graft or local skin flap. We also have observed the screw breakage of ten tibiae but there were no intraoperative jamming or incarceration and no significant complicaton such as bending or breakage of the nail, shortening or non-union of the fractures. In conclusion, unreamed technique is an attractive method to treat the close, displaced fractures of the tibial shaft. 경골 간부 골절의 치료에 있어서 견고한 내 고정으로 조기 관절운동 및 체중 부하를 통해 골절 유합 및 기능 회복을 도모할 수 있는 골수강내 금속정 고정술이 널리 사용되어지고 있다. 골수강내 금속정술의 장점은 술 후 조기 관절운동과 체중 부하가 가능하여 이환율(morbidity)을 감소시키며 불유합의 발생률이 낮아는 것이다. 경골 간부 골절의 치료에 있어서 비확공성 금속정 고정술은 주로 심한 연부 조직 손상을 동반한 골절 및 개방성 골절의 치료에 적용되어지고 있으며, 대부분의 폐쇄성 경골 간부 골절의 치료로는 골수강 확공 후 골수강을 충만시키는 금속정을 삽입하는 확공정 금속정 고정술이 시행되어지고 있는 경향이다. 저자들은 폐쇄성 경골 간부 골절에 대해서 비확공성 금속정 고정술의 유용성 여부를 알아보기 위해서 동국대학교 의과대학 정형외과학교실에서 치료했던 54례를 평균 1년 5개월 추시하여 골 유합 기간, 합병증의 발생에 대해서 분석하였다. 본 연구에서 비확공성 금속정 고정술시 골 유합 기간은 확공성 금속정 고정술시와 비교하여 유의한 차이가 없음을 알 수 있었으며, 확공을 하지 않음으로써 발생할 수 있는 jamming이나 가는 직경의 금속정을 선택함으로써 우려되는 금속정의 휨, 부러짐 등의 합병증은 발생하지 않았다. 결론적으로 폐쇄성 경골 간부 골절의 치료에 있어서도 비확공성 골수강내 금속정 고정술은 유용한 방법으로 사료된다.