Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation

Yoon, Jeong,Juhn, Kyoung-Mi,Yoon, San-Hyun,Ko, Yong,Lim, Jin-Ho The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.1

Objective: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of $Ca^{2+}$ chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). Methods: MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a $Ca^{2+}$ chelator to investigate the effect of $Ca^{2+}$ oscillations on their maturation. Results: As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the $Ca^{2+}$ chelator-treated group. Conclusion: The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by $Ca^{2+}$ chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular $Ca^{2+}$ oscillations driven by fertilization.

미세각막절개 백내장 수술 후 낭내 삽입한 일체형 비구면 인공수정체의 임상 결과

김윤전,전미현,고동아,김재용,김명준,차흥원,Yoon Jeon Kim,Mi Hyun Cheon,Dong Ah Ko,Jae Yong Kim,Myoung Joon Kim,Hung Won Tchah 대한안과학회 2013 대한안과학회지 Vol.54 No.4

Purpose: To evaluate the stability and optical performance of the newly developed single-piece aspheric intraocular lens (IOL) by comparing the clinical outcome of the aspheric IOL with the new optic profile design (HOYA iSert, HOYA iMics) and the aspheric IOL (Akreos MI60), which has been proven effective and safe. Methods: iSert, iMics, and MI60 were inserted into 55 eyes, 60 eyes, and 50 eyes, respectively, after microincision phacoemulsification cataract surgery. Best corrected visual acuity (BCVA), refraction in spherical equivalent, anterior chamber depth (ACD), total higher order aberration (HOA), contrast sensitivity, and surgically induced astigmatism (SIA) were measured and each IOL was evaluated on the functional stability, anterior-posterior stability, centration in the capsular bag, and quality of vision. Results: No statistical differences in preoperative and postoperative BCVA among the 3 IOL groups were observed, however, MI60 showed significant myopic shift postoperatively. Anterior-posterior stability assessed with postoperative change in refractive error and ACD was slightly lower in the MI60 group. In terms of vision quality, while total aberration, total HOA, coma aberration, and contrast sensitivity for the 3 IOLs were not different significantly, spherical aberration of the MI60 group was higher than the other groups at 6 months postoperative. SIA was significantly increased in eyes implanted with iSert than in eyes with iMics or MI60 at 1 month postoperatively, however, the differences were no longer evident after 3 months postoperatively. Conclusions: The new aspheric IOLs, iSert and iMics, showed good stability and visual outcome equal to MI60 at the 6-month postoperative follow-up.

종양의 성장 및 전이에 있어서 NF-κB의 역할

고현미,최정화,나명석,임선영 Official Journal of the Korean society for immunol 2003 Immune Network Vol.3 No.1

제2형 당뇨병 동물모델인 OLETF 쥐에서 장기간 라미프릴 투여가 내당능 및 췌도 베타세포에 미치는 효과

고승현,윤건호,김명미,안유배,송기호,유순집,손현식,차봉연,이광우,손호영,강성구 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:최근 소개된 HOPE(Heart Outcomes Prevention Evaluation)study의 결과에 의하면, 심혈관질환이나 이에 대한 위험인자가 1가지 이상인 9,541명을 대상으로 안지오텐신 전환효소 억제제의 일종인 라미프릴을 투여하여 사망율과 뇌졸증, 심부전, 당뇨병 합병증이 개선되었을 뿐 아니라 다른 항고혈압제에 비해 새롭게 당뇨병으로 진단되는 환자가 줄었음을 보고한바 있다. 이에 저자들은 라미프릴(ramipril)을 제2형 당뇨병 동물모델에 장기간 투여함으로써 당뇨병을 예방하거나 고혈당 상태 또는 인슐린 감수성을 개선시킬수 있는지를 알아보고자 하였다. 방법:생후 24주된 체중 400∼450g의 OLETF와 LETO 쥐를 대상으로 6개월간 라미프릴 경구투여 하였고 혈압, 24시간 단백뇨 및 인슐린 내성검사를 시행하였다. 6개월 이후로는 8주간 투여군과 대조군의 체중을 비슷하게 맞추어 내당능 상태를 평가하였고 이후 다시 8주간 고농도 포도당을 투여하였다. 경구당부하검사를 시행한 후 희생시켜 조직표본을 만들어 guinea pig anti­human insulin 항체로 염색후 DAB로 발색하여 point count 법으로 베타세포를 정량하였고, 면역염색으로 췌도의 변화와 섬유화의 정도를 관찰하였다. 결과:라미프릴을 6개월간 투여하였을 때 OLETF 쥐에서 1)체중 증가가 적었으며 2)경구 당부하검사시 포도당하 면적은 의미있게 감소하였고 3)인슐린 내성검사상 Kitt가 증가되는 경향을 보였으며 4)수축기, 이완기 혈압과 24시간 단백뇨양이 의미있게 감소하였고 5)체중을 맞추면 내당능 상태가 비슷해졌다가 고농도 포도당 주입시 다시 포도당하 면적이 대조군에서 더 증가되었다. 라미프릴 투여군에서 췌도변형 및 파괴와 탈과립, 췌도내 섬유화가 감소하였다. 결론:본 실험에서는 인슐린 비의존성 당뇨병 동물 모델인 OLETF 쥐에서 당뇨병 발생 이전 단계부터 장기간 라미프릴을 투여할 경우 체중증가가 적었고, 투여군과 대조군의 체중을 맞추었을 때는 내당능상태의 차이가 없아가 고혈당 투여시 다시 투여군과 대조군에서 의미있는 차이를 보였다. 라미프릴 투여가 베타세포의 기능면에서 이로운 효과를 보일 것으로 생각되었다. 따라서 비만한 환자에서 안지오텐신 전환효소 억제제의 장기투여는 단백뇨 감소, 혈압강하 효과 이외에 체중증가의 억제 및 당뇨병 발생을 억제할 가능성을 시사한다. Background : In a Heart Outcomes Prevention Evaluation HOPE study, ramipril, a long-acting angiotensin-converting enzyme (ACE) inhibitor, significantly reduced the death rates the number of myocardial infarctions, strokes, heart failure as well as the risk of complications related to diabetes and of diabetes itself. However, it is known that ACE inhibitors improve glucose tolerance or insulin sensitivity or reduce the incidence of diabetes. Methods : 24 week-old OLETF (Otsuka Long Evans Tokushima Fatty) rats weighing 400 ~ 450 g were used in this study. 4 groups of rats were examined in parallel for 40 weeks. The OLETF rats were randomized for treatment with an aqueous solution of ramipril (5 mg/Kg) daily [OL (RMP), n=10)] and with saline [OL (CON), n=10)]. The LETO rats were also randomized in the same was as the OLETF rats (LT(RMP), n=10, LT (CON), n=10). The blood glucose level, body weight, systolic and diastolic blood pressure was assessed every month. At 3 and 6 months, the 24hrs urinary protein concentration was measured, and as insulin tolerance test and oral glucose tolerance test were conducted in all experimental groups. After 6 months, the body·weight was matched for 2 months in each corresponding group. Subsequently, a 15% sucrose loading was done for 2 months. After the glucose tolerance test, the pancreas was excised and immuno histochemical staining was conducted for insulin to quantify the beta cell mass by a point-counting method. In addition, the islet morphology was evaluated in the pancreas. Results : Ramipril treatment for a period of 6 months improved the 2hr blood glucose level, the area under the glucose curve in the oral glucose tolerance test, insulin sensitivity in addition to lowering significantly systolic and diastolic blood pressure and 24hrs urinary protein level significantly in OLETF rats. Of note, a lower weight gain was observed in both the ramipril-treated animals at 6 months. After weight matching, the AUC g and 2hr blood glucose level values were similar between the corresponding groups, but a 15% sucrose loading worsened the AUC g value. Histologically, the islets were less disorganized and the extent of fibrosis was lower in the ramipril-treated OLETF rats in the trichrome stain. Conclusion : Long-term treatment of ramipril, a long acting angiotensin-converting enzyme inhibitor may be useful for suppressing weight gain and proteinuria in addition to having a protective effect on the islet to harmful stimuli such as hyperglycemia (J Kor Diabetes Asso 25:469~482, 2001).

신경인성 과반사성 배뇨근에 대한 배뇨근 내보툴리눔 독소 A 주사

고현윤,신용범,손현주,장재혁,이경미,문혜정,하용훈 부산대학교 병원 암연구소 2008 부산대병원학술지 Vol.- No.23

Local injections of botulinum toxin A have been reported in the treatment of neurogenic bladder dysfunctions including detrusor sphincter dyssynergia and detrusor overactivity. We reports a case of intradetrusor injection of botulinum toxin type A in acervical cord injured patient with neurogenic overactive detrusor refractory to high dose anticholinergic medication. Botulinum toxin A (200 units) was injected cystoscopically into the detrusor in 40 sites over the bladder with sparing the trigone. Urodynamic studies were performed at baseline, and eight days, four weeks, and eight weeks after intradetrusor injection. There was marked improvement of the urodynamic parameters including maximum cystometric capacity and maximum detrusor pressure after intradetrusor botulinum toxin injection.

유아 멀티미디어 교육 활성화를 위한 방향

서현아,고은미 경성대학교 2000 論文集 Vol.21 No.2

The Education of Multimedia in the kindergarten become more and more important to young children these days. However most educators didn't integrate multimedia activities to their other parts of the classroom. The purpose of this study is to recognize the importance of multimedia education for young children and promote teacher's positive role. Accordingly, this study examined computer activity, software, use of internet and the factors the influence teacher to integrate computer technology into the teaching and learning process. The results of this study are as follows, 1) Multimedia should be understood by the concept of multimedia computer and interacting environment. 2) The important thing in computer activity is not controversy about computer effects but how we connect computer activity into children's life well. 3) Developmentally appropriate software is essential part of successful computer activities. 4) Developmentally appropriate internet program may serve as an interesting, creative platform in the acquisition of general skills. 5) External factors and intern릴 factors should be considered together for early multimedia education and integrate multimedia activity to children's environment.

물질남용자의 유형화에 대한 예비적 연구

김현수,조선미,이용석,임기영,이호영 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.1

목 적: 본 연구는 물질남용자들의 인구학적 특성, 물질남용 특성, 인성특성을 파악하는 것을 통해 물질남용자들의 유형화를 하기 위하여 예비적으로 시행된 것이다. 방 법: 본 연구자가 수행하는 집단프로그램에 참여한 사람들을 직접 면접하고, MMPI, BDI, 이화방어기제검사 및 충동성검사 등을 시행하고 이에 대한 빈도분석과 Chi-square 검증 그리고 문항에 따라 F-검증을 실시하였다. 결 과: 1) 본 집단은 대마가 14명, 필로폰이 26명, 유해화학이 2명이었으며, 1년 이상 사용한자가 27명(64.35%) 이었고, 사회경제적으로 중이하가 많았으며 20-30대가 32명(76.5%)이었다. 학력 및 경제적 상태는 다양했으며, 직업도 다양했다. 전과는 15명이었다. 2) 발달력 상 부모관계가 나쁘거나 상실하거나, 이혼, 분리 등의 경험이 많으며, 상관분석상 전과는 비행력과 비행력은 부모약물, 부모관계, 전과와 유의미한 상관이 있었다(p<0.05). 3) MMPI 군집상 두 집단으로 분류가 되었으며 한 집단은 단순약물사용군으로, 다른 집단은 사회병질집단으로 명명하였다. 두 집단과 발달력과의 상관에서 사회병질집단은 부모관계, 부모약물과 통제적으로 유의미했다(p<0.05). 4) 물질종류에 따른 MMPI 척도에서 Mf, Si 척도가 유의미하게 상승했으며, 부모상실, 부모이혼, 부모학대가 있는 집단은 MMPI 척도에서 Pd 척도가 유의미하게 상승했다(p<0.05). 결 론: 이상의 결과로 볼 때 발달력, 물질종류에 따른 유형화의 가능성이 향후 더 연구되어져야 할 것으로 생각이 되며 이를 치료프로그램과 연결하는 연구가 앞으로 수행되어져야 할 것이다. Objectives: This preliminary study was performed to typify a group of substance abusers by their demographic data, developmental history, substance use patterns, and personality profiles. Methods: Forty-two substance abusers were assessed with Minnessota Multiphasic Personality Inventory(MMPI), Ehwa-Defense Mechanism Test, Beck Depression Inventory(BDI), Beck Anxiety Inventory(BAI), etc. They also participated in semi-structured interview by psychiatrists and social workers. Results: 1) This group consisted of 14 cannabis users, 26 methamphetamine users, and 2 inhalant users. Twenty-seven(64.35%) of this group abused drugs over one year. Thirty-two(76.5%) of the subjects were in their twenties or thirties. Many of them were less than low in their economic status. Their academic careers and occupations were various. Fifteen abusers had past criminal histories. 2) Among their developmental history, parental alcohol problem, poor relationship with parent, and criminal history were significantly correlated with one another(p<.05). 3) In the cluster analysis upon their MMPI profiles, two clusters emerged; one cluster presented low profiles, and the other showed high profiles. We named the former simple drug user group, and the latter `sociopathic drug abuser group`(p<.05). 4) There differences in MMPI profiles according to the kind of drugs and developmental problems. Methamphetamine group was higher in Mf and Si. The group with poor developmental history was higher in Pd profiles(p<.05). Conclusion: These results suggest that, for typology of substance abusers, more investigation should be made on developmental history and drug use patterns. This study presented two clusters of simple abuse group and sociopathic abuse group.

RAPD와 핵 리보솜 DNA ITS2 염기서열에 의한 한국산 전나무속 식물의 유전적 변이 및 계통유전학적 분석

정용환,한상현,오유성,고미희,고석찬,김문홍,오문유 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.2

We analyzed the genetic variation and phylogenetic relationship among fourteen taxa of GenusAbies distributed in Korea using random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) 2 sequences of the nuclear ribosomal DNA (rDNA). RAPDs were used for identification of selected taxa by amplification using single 10-mer primers of arbitrary sequence. Fourteen taxa were clearly classified with 10 arbitrary primers, which generated 102 useful polymorphic bands that were evaluated according to the presence or absence of the fragments of equal size. The genetic distance between A. koreana for. chlorocarpa and A. koreana for. rubrocarpa was 0.127, showing the closest among any other pairs. By comparing the base sequences in the ITS2 region of the nuclear rDNA, genetic relationship was assesed among selected taxa. It showed twenty six sites of point mutation (base substitution) in 243 base pairs (bp) of ITS2 region. The G+C contents of ITS2 region were ranged from 58.3% to 61.7%. The value of nucleotide divergence among selected taxa was ranged from 0.4% to 9.8%. These results showed that RAPD and ITS2 sequence analysis was a useful tool for elucidating phylogenetic relationship and selection of specific marker in Abies species.

신라대학교 주변 백양산의 동물상

노치권(외 6명) 신라대학교 자연과학연구소 2012 自然科學論文集 Vol.22 No.-

To study fauna of Silla University located in Mt. Baek-yang, we conducted six surveys from 29 May 2011 to 28 April 2012. We found five phyla 41 orders 197 species of animals inhabiting in this area: four phyla 27 orders 163 species of invertebrates and in vertebrates one order six species of fishes, three orders nine species of amphibians and reptiles, six orders 14 species of aves, and four orders five species of mammals. Of these species, some Korean endemic species were included: a fish of Rhodeus uyekii in a pond near main gate of this university, a frog of Rana coreana in a creek near a dormitory, and a rabbit of Lepus coreanus and a water deer of Hydropotes inermis in a grass region near a main stadium. A kind of hawk, Falco tinnunculus as a natural monument (animal) of no. 323-8, was observed on a building of medical and life science. A blue frog of Hyla japonica as a variation was found near a spring resort. Therefore, this area is considered an ecologically important area having a high animal diversity.

한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.