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세포 내 Ca2+ 변화를 이용한 계면활성제의 가려움 유발 가능성 연구
최지예 ( Ji Yei Choi ),조선아 ( Sun A Cho ),김현나 ( Hyun Na Kim ),김지훈 ( Ji Hoon Kim ),장원희 ( Won Hee Jang ),이존환 ( John Hwan Lee ),안수선 ( Su Sun An ) 한국동물실험대체법학회 2014 동물실험대체법학회지 Vol.8 No.1
Itching is the unpleasant sense that urges to scratch the skin, and appears to vary according to the individual for the same stimulus. In recent animal studies, it has been reported that itching is caused by the use of anionic surfactants. Therefore, the itching sensation by ingredients and wash-off products containing various surfactants was assessed by use of in vitro method in this study. The change of intracellular Ca2+ level which included in the itch-inducing pathway was measured in human keratinocyte cell line (HaCaT) and human neuroblastoma cell line (SH-SY5Y). The influx of Ca2+ was increased in anionic surfactants and amphoteric surfactants rather than nonionic surfactants. In addition, according to increasing of anionic surfactants in wash-off products, intracellular Ca2+ level was elevated. This study could be useful for the development of safe wash-off products.
연구논문 : 세포 내 Ca2+ 변화를 이용한 계면활성제의 가려움 유발 가능성 연구
최지예 ( Ji Yei Choi ),조선아 ( Sun A Cho ),김현나 ( Hyun Na Kim ),김지훈 ( Ji Hoon Kim ),장원희 ( Won Hee Jang ),이존환 ( John Hwan Lee ),안수선 ( Su Sun An ) 한국동물실험대체법학회 2014 동물실험대체법학회지 Vol.8 No.1
Itching is the unpleasant sense that urges to scratch the skin, and appears to vary according to the individual for the same stimulus. In recent animal studies, it has been reported that itching is caused by the use of anionic surfactants. Therefore, the itching sensation by ingredients and wash-off products containing various surfactants was assessed by use of in vitro method in this study. The change of intracellular Ca2+ level which included in the itch-inducing pathway was measured in human keratinocyte cell line (HaCaT) and human neuroblastoma cell line (SH-SY5Y). The influx of Ca2+ was increased in anionic surfactants and amphoteric surfactants rather than nonionic surfactants. In addition, according to increasing of anionic surfactants in wash-off products, intracellular Ca2+ level was elevated. This study could be useful for the development of safe wash-off products.
A biocompatible implant electrode capable of operating in body fluids for energy storage devices
Chae, Ji Su,Heo, Nam-Su,Kwak, Cheol Hwan,Cho, Wan-Seob,Seol, Geun Hee,Yoon, Won-Sub,Kim, Hyun-Kyung,Fray, Derek John,Vilian, A.T. Ezhil,Han, Young-Kyu,Huh, Yun Suk,Roh, Kwang Chul Elsevier 2017 Nano energy Vol.34 No.-
<P><B>Abstract</B></P> <P>Implantable electronic medical devices (IEMDs) can potentially be used to solve various clinical problems including the monitoring of chronic diseases and electro-organ transplantation. Several recently introduced techniques based on implantable devices that exploit novel metal- or carbon-based hybrid materials are biocompatible owing to their encapsulation in nontoxic polymers. However, such techniques limit the correct functioning of implantable devices, resulting in frequent replacement, difficult miniaturization, and inflammatory side effects in the body. Here, we describe a new technique for application to IEMDs that is capable of providing energy storage using the natural ions of body fluids as electrolytes in a supercapacitor (or ultracapacitor). The system is constructed with a solar cell for energy harvesting and a supercapacitor for energy storage. We assembled IEMDs with two biocompatible electrodes, specifically, MnO<SUB>2</SUB> nanoparticles affixed to multi-walled carbon nanotubes as the positive electrodes and phosphidated activated carbon as the negative electrodes. From the obtained result, this work can be further extended to the use of rats. This technique avoids the problems of performance degradation and toxicity that normally limits the reaction that is permissible in extracellular fluid. We present this concept schematically. The two biocompatible electrodes were successfully implanted into the subcutaneous layer of a rat's skin with both electrodes showing stable performance in use as parts of a supercapacitor. These findings establish a platform for potential biocompatible materials for implantable energy storage devices.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
신현수(Hyun Soo Shin),이형식(Hyung Sik Lee),장세경(Sei Kyung Chang),정은지(Eun Ji Chung),김진희( Jin Hee Kim),오윤경(Yoon Kyung Oh),전미선(Mi Sun Chun),허승재(Seung Jae Huh),노준규(John Jun Kyu Loh),서창옥(Chang-Ok Suh) 대한방사선종양학회 2004 Radiation Oncology Journal Vol.22 No.3
목 적: 유방암의 치료에서 표준화된 치료와 방사선치료의 적정 사용을 제고하기 위하여 유방암으로 진단받은 환자 들의 치료 현황을 전국적으로 조사하였다. 특히 유방암의 최신 치료법이고 방사선치료가 필수적인 유방보존술의 시행 빈도를 분석함으로써 우리나라 유방암 치료 수준을 평가하고 개선점을 찾고자 하였다. 대상 및 방법: 1998년 1월부터 1998년 6월까지 유방암으로 최초 진단받은 환자들의 임상 특성과 치료 상황을 조사하였다. 조사대상 병원은 전국 병원을 지역별, 병원군별(3차병원 대 종합병원), 방사선치료시설 유무로 구별하여 표본병원으로 선정하였다. 총 27개 병원에 연구원들이 직접 방문하여 의무기록지와 암등록지를 토대로 조사표를 작성하였다. 결 과: 유방암환자 총 1,048명에 대한 조사결과는 다음과 같다. 연령별 분포는 40대가 33.4%로 가장 많았고 50대가 28.5%, 30대가 22%이었으며 평균연령은 46.4세였다. 수술 후 병기 분포는 AJCC stage II가 58.4%, stage I이 23.5%, stage 0가 6%이었으며 stage II 이하로 조기에 진단되는 비율이 87.8%였다. 전체 환자의 64.8%가 전유방적출술을 받았고 26%의 환자가 유방보존적 수술을 받았으며 4.5%는 항암화학요법을 받은 후 수술을 하였고 4.6%의 환자들은 수술을 시행하지 않았다. AJCC 병기 0기에서는 47.5%, 1기에서는 54.4%, II기에서는 20.3%가 유방보존술을 받았다. 유방보존술 후 방사선치료 시행율은 1기 93.4%, II기 89.9%, III기 33.3%이었고 유방절제술 후 방사선치료는 III기의 45%에서 시행되었다. 지역과 방사선치료시설 유‧무에 따라서 유방보존술 비율이 달랐다. 결 론: 방사선치료의 활용도가 적정 수준에 미흡했는데 이는 의료진의 인식 부족과 주변에 활용가능한 방사선치료 시설의 부족, 경제적 여건 등이 원인으로 생각된다. 유방보존술의 활용도 역시 선진국 수준에는 미치지 못하고 있는데 이는 우리나라 유방암환자들이 유방을 보존하고자 하는 욕구가 적고 유방보존술의 장점에 대한 인식이 부족하기 때문으로 생각된다. 또한 방사선치료 시설이 없는 종합병원에서 유방보존술 시행 빈도가 상대적으로 적은 것을 관찰할 수 있었는데 주변 여건에 따라서 치료 방침이 결정될 수 있음을 알 수 있었다. Purpose: In order to improve the proper use of radiotherapy and breast-conserving treatment (BCT) in the management of breast cancer, current status of breast cancer treatment in Korea was surveyed nationwide and the use of BCT were evaluated. Materials and Methods: Patients characteristics and treatment pattern of 1048 breast cancer patients from 27 institutions diagnosed between January, 1998 and June, 1998 were analyzed. The incidence of receiving BCT was analyzed according to the stage, age, geography, type of hospital, and the availability of radiotherapy facility. Results: Radical mastectomy was performed in 64.8% of total patients and 26% of patients received breastconserving surgery (BCS). The proportions of patients receiving BCT were 47.5% in stage 0, 54.4% in stage I, and 20.3% in stage II. Some of the patients (6.6% of stage I, 10.1% of stage II and 66.7% of stage III) not received radiotherapy after BCS. Only 45% of stage III patients received post-operative radiotherapy after radical mastectomy. The proportion of patients receiving BCT was different according to the geography and availability of radiotherapy facilities. Conclusion: Radiotherapy was not fully used in the management of breast cancer, even in the patients received breast-conserving surgery. The proportion of the patients who received BCT was lower than the report of western countries. To improve the application of proper management of breast cancer, every efforts such as a training of physicians, public education, and improving accessibility of radiotherapy facilities should be done. The factors predicting receipt of BCT were accessibility of radiotherapy facility and geography. Also, periodic survey like current research is warranted.
유화동결건조법으로 제조된 HA/PLGA 지지체를 이용한 연골 재생
정수현 ( Su Hyun Jung ),장지욱 ( Ji Wook Jang ),김순희 ( Soon Hee Kim ),홍현혜 ( Hyun Hye Hong ),오아영 ( A Young Oh ),이종문 ( John M. Rhee ),강영선 ( Young Sun Kang ),강길선 ( Gil Son Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4
Adult articular cartilage tissue has poor capability of self-repair. Therefore, a variety of tissue engineering approaches are motivated by the clinical need for articular repair. PLGA and hyaluronic acid(HA) has been widely used as biocompatible scaffolds materials to regenerate tissue. HA loaded PLGA scaffolds were prepared by a emulsion freeze-drying method. The chondrocytes were seeded on the HA-PLGA scaffolds and measured by MTT assay. Morphological observation, histology, biological assay for collagen and sGAG, and PCR were performed. In MTT assay result, scaffolds containing HA were higher cell viability then only PLGA scaffolds. Although collagen, sGAG, mRNA and collagen type II were greater than HA-PLGA scaffolds and collagen type I was less than HA/ PLGA scaffolds. When we cultured cartilage cell of rabbit in vitro, we observed better to keep the characteristic of cartilage cell in the HA-PLGA scaffolds than that PLGA scaffolds. This study suggests that HA/PLGA scaffold may serve as a potential cell delivery vehicle and a structural basis for in vitro tissue engineered articular cartilage.
Unilateral Bronchospasm during Induction of Anesthesia
( Soo Kyung Lee ),( Hyun Ji John ),( Eun Ji Youn ),( Hyun Soo Moon ),( Hwa Ja Kang ) 경희대학교 경희의료원 2018 慶熙醫學 Vol.33 No.1
We report a healthy woman of whom the right lung was unable to be inflated during induction of anesthesia and after endotracheal intubation. Failure to ventilate one side of the lung after endotracheal intubation occurs commonly and can promptly be identified by routine examination of the chest by inspection and auscultation. The most probable cause is unilateral endobronchial intubation. Because unilateral bronchospasm is a relatively rare condition, it is easy to misdiagnose. Unilateral bronchospasm should be considered a differential diagnosis in a patient with decreased or absent unilateral lung sounds. Early diagnosis is the key for successful management.
( Soon Hee Kim ),( Ji Wook Jang ),( Su Hyun Jung ),( Jin Hee Choi ),( Hyun Jung Ha ),( John M. Rhee ),( Young Sun Kang ),( Gilson Khang ) 한국조직공학·재생의학회 2008 조직공학과 재생의학 Vol.5 No.4
This study aimed to evaluate the chondrogenic potential of alginate gel loaded transforming growth factor beta 1(TGF-β1) and bone marrow derived stem cells(BMSCs). We, based on previous in vitro experiment, encapsulated cell and TGF-β1 into alginate bead and transplanted on the dorsum of nude mouse subcutaneously. The implantations were harvested and evaluated by histology through H&E, Alcian Blue, Safranin-O and immunohistochemistry( IHC) and immunofluorescence(IF) staining. These results presented that the alginate bead incorporated with TGF-β1 and BMSC is promising for cartilage tissue engineering application and that specially is more helpful to chondrogenesis in a limited area than alginate with nothing or the alternative TGF-β1 or BMSC.
Helicobacter pylori 감염 진단에서의 직접 연쇄중합반응법(Direct Polymerase Chain Reaction)의 역할
김정원,신지현,이종화,이문숙,서은옥,송일한,김정택,노임환,진영주,양미라,이대준,임창영 대한소화기학회 1998 대한소화기학회지 Vol.31 No.3
Background/Aims: A variety of diagnostic procedures are available for the identification of Helicobacter pylori in clinical sarnples. Recently, the detection of H. pylori in gastric biopsy samples was attempted using polymerase chain reaction, in which primers based on the sequence of a species specific antigen of H. pylori were used. The aim of this study is to test whether the PCR is useful for the detection of H. pylori. Methods: Four hundred forty gastric biopsy samples were tested. Urease test was performed and then PCR using the primer of 298 bp sized 26 kDa antigen was taken in the same 2 pieces of gastric biopsy samples. Results: Total concordance rate between the urease test and direct PCR test was 85.9%. The concordance rate between the two tests were 80.2%, 88.5% and 88.8% in gastric cancer (n=86), gastric ulcer (n=78) and duodenal ulcer (n=161) respectively. The positive rates of the urease test and the direct PCR test were 36.0% and 44.2%, respectively in the gastric cancer, 56.4% and 55.1%, in the gastric ulcer, and 84.5% and 82.0% in the duodenal ulcer. The positive rate of H. pylori infection in the gastric cancer was lower than those of the other gastroduodenal diseases. conclusions: Tbe PCR can detect H. pylori successfully in gastric biopsies using tbe 26 kDa antigen gene. The PCR technique have potential benefits of higher sensitivity and quicker detection than the urease test. Further study is necessary to establish PCR test as a diagnostic method of H. pylori and to elucidate the characteristics of H. pylori.