갑상선 결절에 동반된 퇴행성 낭종의 재발과 낭액내 VEGF의 관련성

조은호,박희붕,김현만<SUP>1<.SUP>,이관우<SUP>1<.SUP>,정윤석<SUP>1<.SUP>,소의영,Eun Ho Cho,M.D.,Hee Boong Park,K.D.,Hyun Man Kim,M.D.<SUP>1<.SUP>,Kwan Woo Lee,M.D.<SUP>1<.SUP>,Yun Suk Jung,M.D.<SUP>1<.SUP> and Euy Young 대한갑상선-내분비외과학회 2002 The Koreran journal of Endocrine Surgery Vol.2 No.1

Purpose: VEGF, a potent angiogenic factor, increases vascular permeability and induces the fluid accumulation in the peritoneal and pleural cavities. This study is designed to know whether the VEGF concentration are associated with the formation of cystic fluid of thyroid nodules and the results of treatment. Methods: To know whether thyroid follicular cells produce VEGF, we measured the VEGF concentration in the conditioned medium after primary culture of the normal thyroid follicular cells (NT 1.0). Thirty seven patients, who had visited Ajou University Medical Center, were divided into two groups; group A (n=19) is patients whose cystic nodules disappeared completely with 1 or 2 times fine needle aspirations. In Group B (n=18), the cystic nodules recurred rapidly and required aspiration more than 3 times. Clinical records of patients were analyzed and compared with TSH and VEGF levels in cystic fluids. Results: The VEGF concentration in basal conditioned medium were 8.2⁑1.2 ng/ml in NT 1.0 and 8.2⁑0.6 ng/ml in thyroid cancer cell line (FTC-133). NT 1.0 was not affected by TSH stimulation. Mean concentration of TSH of all cystic fluids was 4.36μIU/ml (0.13∼21.7) and there was no difference between 2 groups. Mean concentration of VEGF of all cystic fluids was 140.7 ng/ml (11.1∼688.8). The VEGF concentration (287.9⁑289.6 ng/ml) in group B was significantly higher than that (104.2⁑97.1 ng/ml) in group A (P<0.05). Conclusion: This study suggests that thyroid follicular cells produce and secrete VEGF, and VEGF is related with the accumulation of cystic fluid in degenerative cysts of thyroid. Cysts with high VEGF concentration promote rapid reaccumulation of the cystic fluid and possibly necessitate operation in selected patients. (Korean J Endocrine Surg 2002;2:25- 30)

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

FCT 5 : Fractional laser-assisted topical imiquimod 5% cream treatment for recalcitrant common warts in children

( Hyun Chang Ko ),( Jeong Min Kim ),( Hyun Ho Cho1 ),( Won Jeong Kim ),( Je Ho Mun ),( Margaret Song ),( Hoon Soo Kim ),( Byung Soo Kim ),( Moon Bum Kim ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Conventional destructive modalities for warts like cryotherapy or laser ablation have some limitations that are excruciating pain during procedure, in especially pediatric patients. Imiquimod is a topical immune response modifier that was approved for treating genital and perianal warts. But, thick stratum corneum of common warts may act as a barrier against drug permeation via the skin. Objectives: To evaluate efficacy and safety of above treatment. Methods: Eleven pediatric patients (6 female and 5 male, mean age 12.5 years) were included in this study. The lesions were treated by fractional 2940 nm Er:YAG laser for achieving the penetration of stratum corneum with 1 or 2 weeks intervals. Then, imiquimod 5% cream was self-applied once daily for 5 days a week. Assessment of response and adverse effects was performed every 2 weeks until complete clearance or up to maximum of 48 weeks. Pain during procedure was checked by VAS (0-10). Results: 8 out of 11 (72.7%) patients experienced complete clearance of all warts lesions. Mean duration of total treatment was 29.7 (16-48) weeks. Mean number of fractional laser treatment was 17.5 (8-37) times. No significant adverse effect was observed. VAS score of the pain during fractional laser treatment was 2.4 (1-4), comparing with 6.2 (5-8) during cryotherapy. Conclusion: Fractional ErYAG laser-assisted topical 5% imiquimod cream is an effective and safe treatment option for recalcitrant common warts of the children do not tolerate pain well.

A Case of Favorable Responses after Gefitinib in a Patient with EGFR Mutated Adenosquamous Lung Carcinoma

Nam Jun Cho,Ho-Sung Lee,Si-Hyong Jang,Jae Sung Choi,Ju Ock Na,Ki Hyun Seo,Yong Hoon Kim,Hyo-Chul Kang,Dae-Yeon Kim1 순천향의학연구소 2013 Journal of Soonchunhyang Medical Science Vol.19 No.2

In patients with advanced non-small cell lung cancer harboring epidermal growth factor receptor (EGFR) mutations, the epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are used as first treatment option. Because adenosquamous carcinoma (ASC) is a rare histologic subtype, evidences about EGFR-TKIs as first treatment option for advanced ASC are lacking. We report a case of an advanced ASC patient with the EGFR mutation, who showed good responses during 4-month treatment with gefitinib. And we will review about a necessity of EGFR mutation test and efficacy of EGFR-TKIs in ASC patients from the recent studies.

What SERVPERF in Recruiting Website Does Determine Users` Perceived Value, Satisfaction, and Revisit Intention in Korea?

( Chul-ho Cho1 ),( Jae Hoon Hyun ) 한국품질경영학회 2015 한국품질경영학회 학술대회 Vol.2015 No.2

Purpose This study aims to identify the factors of the recruiting websites’ service quality that determines the users’ perception and behavior and the moderating role of job exploration type in Korea. Design/Methodology/Approach This research is designed to address the casual relation between service quality of recruiting websites and revisit intention by introducing perceived value and user satisfaction as mediating variables. Based on pilot tests and previous studies of website quality, we devised a SERVPERF evaluation model pertaining to the web service quality of recruiting website. 302 valid observations were collected and used to examine the constructs through structural equation modeling. Findings The findings show that interactivity, aesthetics, reliability and usability influence service quality of recruiting websites through perceived value and satisfaction as mediators. This study also found that job exploration type moderates, as a user group seeking full time jobs recognizes value from the quality of information and scope of interactivity, while users searching for part time jobs appreciate aesthetics. Implications This research contributes to the literature by the provision of the elements that influence service quality of recruiting websites and by examining perceived value as a new mediator. The findings also provide practical implications for recruiting websites to tune strategic approach by different motives of user groups. Originality/Value A number of previous studies examined web service quality and users’ reaction in the area of e-commerce and general websites but there were lack of attempts to identify service quality in the context of online recruitment. This study fills the research gaps by providing the SERVPERF evaluation model examining the elements that influence quality of recruiting website and identifying how service quality affects users’ perception and behaviour.

Effect of Different Inoculation Concentration of Escherichia coli on Boar Sperm Quality and Reproductive Performance in Sow

Soo Jin Sa,Sun Ho Choi,Hyun Jong Kim,Kyu ho Cho,Joon Ki Hong,Du Wan Kim,Young Hwa Kim,Jun Cheol Park1,Ki Hwa Chung 한국동물생명공학회(구 한국동물번식학회) 2014 Reproductive & developmental biology Vol.38 No.4

The objective of this study was to determine the effects of E. coli on boar sperm quality and reproductive performance in sows after artificial insemination. Three different levels of E. coli were artificially inoculated to semen with following concentrations; Control, 500, 5,000 and 50,000 colony forming unit (cfu)/ml. Semen samples were preserved at 17℃ for 5 days. Sperm motility was significantly decreased (p<0.05) on day 3 in the group inoculated with 5,000 cfu/ml compared to control groups. In all treatment groups, sperm motility was gradually decreased as storage time increased, but the decline pattern was more drastic in the groups inoculated with 5,000 and 50,000 cfu/ml groups from day 3 (p<0.05) compared to control group. After 3 day of storage at 17℃, sperm viability in sample inoculated with the highest concentration (50,000 cfu/ml) of bacteria was less (p<0.05) than that of control group. The pH of semen sample pH was maintained 7.2~7.5 in all groups during the experimental period. No differences (p>0.05) were found for both storage time and bacterial concentration. The pregnancy rate and live born piglets tend to decrease by increasing the concentration of E. coli in semen. In particular, the rate of pregnancy was lower in the group inoculated with 50,000 cfu/ml (58.3%) compare to the other groups (81.8, 75.0, 76.5%). These results suggest that the contamination of E. coli in boar semen negatively affects fertilizing ability of boar sperm and the reproductive performance obtained from sows after artificial insemination.

Infliximab으로 호전된 장관 증상없이 발생한 구강-안면 크론병

정보용 ( Bo Yong Jung ),이석호 ( Suck Ho Lee ),정승규 ( Seung Kyu Chung ),이창균 ( Chang Kyun Lee1 ),이태훈 ( Tae Hoon Lee ),정일권 ( Il Kwun Chung ),김선주 ( Sun Joo Kim ),조현득 ( Hyun Deuk Cho ) 대한소화기학회 2012 대한소화기학회지 Vol.59 No.6

Crohn`s disease is a chronic inflammatory bowel disease that can involve the whole gastrointestinal tract. The orofacial manifestation of Crohn`s disease, which is rare, can develop irrespective of intestinal involvement. These orofacial lesions are often misdiagnosed as simple oral ulcers. Corticosteroids are the mainstay of therapy for orofacial Crohn`s disease. However, infliximab, the chimeric monoclonal antibody to tumor necrosis factor-α, is now considered as a primary treatment because of the disease`s relatively high rate of steroid resistance. We present a case of deep oral ulcer and periorbital swelling in a 65-year-old woman. She was diagnosed with intestinal Crohn`s disease 7 years ago, which was in remission after treatment with an immunosuppressive agent (azathioprine). The patient was given the diagnosed with orofacial Crohn`s disease and successfully treated with infliximab. (Korean J Gastroenterol 2012;59:437-440)