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Choi, Jin Woo,Kim, Hyeonjin,Kim, Hyo-Cheol,Lee, Yunjung,Kwon, Jeongmin,Yoo, Roh-Eul,Cho, Hye Rim,Choi, Seung Hong,Chung, Jin Wook Potamitis Press 2013 Anticancer research Vol.33 No.5
<P>To evaluate the feasibility of carbogen-challenge blood oxygen level-dependent (BOLD) magnetic resonance imaging (MRI) for assessing the early response of liver tumors to chemoembolization in a rat hepatoma model.</P>
Choi, Seung Hong,Cho, Hye Rim,Kim, Hoe Suk,Kim, Young Hwa,Kang, Keon Wook,Kim, Hyeonjin,Moon, Woo Kyung John Wiley Sons, Ltd 2012 NMR in biomedicine Vol.25 No.5
<P>Cellular MRI with a reporter gene offers the opportunity to track small numbers of tumor cells and to study metastatic processes in their earliest developmental stages in the target organs of interest. This study demonstrates the feasibility of using the MR reporter ferritin for the noninvasive imaging and quantification of metastatic melanoma cells in the lymph nodes (LNs) of living mice. A B16F10 murine melanoma cell line expressing human ferritin heavy chain (hFTH) and green fluorescent protein (GFP) was constructed to allow the detection of cells by MRI and fluorescence imaging. Stable overexpression of hFTH and GFP in B16F10 murine melanoma cells was feasible and showed no cellular toxicity. In addition, hFTH cells were detectable by 9.4‐T MRI <I>in vitro</I> and <I>in vivo</I>, yielding significant changes in <I>T</I><SUB>2</SUB>* relative to control cells. In BALB/c nude mice, the presence of hFTH‐ and GFP‐expressing metastatic melanoma cells in deep‐seated axillary LNs was demonstrated as areas of low <I>T</I><SUB>2</SUB>* on MRI, but the same LNs were not visible by fluorescence imaging because the light was unable to penetrate the tissue. Furthermore, the metastatic volume of each LN, which was assessed by cumulative histogram analysis of the <I>T</I><SUB>2</SUB>* MRI data, correlated well with tumor burden, which was determined by histology (<I>r</I> = −0.8773, <I>p</I> = 0.0001). This study is the first to use MRI and an MR reporter gene for both the visualization and quantification of metastatic cancer cells in LNs. Copyright © 2011 John Wiley & Sons, Ltd.</P>
Eom, Hyeonjin,Kim, Jae-Han,Hur, Junyoung,Kim, Taek-Soo,Sung, Sang-Keun,Choi, Jun-Hyuk,Lee, Eungsug,Jeong, Jun-Ho,Park, Inkyu American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.45
<P>Metal thin film electrodes on flexible polymer substrates are inherently unstable against humidity and mechanical stresses because of their poor adhesion properties. We introduce a novel approach for improving the adhesion characteristics of metal–polymer interface based on the nanostructuring of the polymer substrate by using nanoimprint lithography. The adhesion characteristics of metal–polymer interface were measured by accelerated test, cyclic bending test and double cantilever beam (DCB) test. The interface of Au/Ti dual layer thin film and nanoimprinted PMMA substrate shows over 2.03 and 1.95 times higher adhesion energy (<I>G</I><SUB>c</SUB>) than that of Au/Ti dual layer thin film and plane PMMA substrate in air and wet environments, respectively. The adhesion energy between metal thin film and polymer substrate was dramatically improved by the increased surface roughness and mechanical interlocking effect of numerous nanoscale anchors at the edges of nanoimprinted surface, which was verified by both experiment and numerical analysis.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2015/aamick.2015.7.issue-45/acsami.5b06631/production/images/medium/am-2015-06631q_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am5b06631'>ACS Electronic Supporting Info</A></P>
Microchip상에서 효율적인 DNA 분석을 위한 반복단위 단백질의 생산
이현진(Hyeonjin Yi),최석진(Seok Jin Choi),서태석(Tae Seok Seo),원종인(Jong-In Won) 한국생물공학회 2010 KSBB Journal Vol.25 No.2
Drag-tag으로 사용될 반복단위 단백질을 생물학적인 방법을 통해 생산함으로써 수용액 내에서 DNA 분리가 가능함을 확인하였다. 서로 다른 크기를 갖는 두 종류의 반복단위 단백질을 디자인하였고, 이를 발현시킨 뒤 정제하였다. 정제된 반복단위 단백질에 형광 dye를 포함하고 있는 100 base의 DNA를 연결하였고, 이 연결 물질을 모세관 내부가 수용액으로 충진된 microchip 상에서 전기영동 하였다. 그 결과 생물학적으로 생산된 반복단위 단백질이 SNP 분석과 같은 빠르고 효율적인 DNA 분석에 적합한 후보물질로 사용될 수 있음을 확인하였다. We generated the feasibility of DNA separation in free-solution using genetically engineered repetitive polypeptides as drag-tags. Two different-sized repetitive polypeptides were designed, expressed in E. coli, and purified. They were conjugated to a fluorescently labeled DNA (100 base), and the electrophoretic mobilities of these conjugate molecules were analyzed on a microchip. The results of these studies indicate that genetically engineered repetitive polypeptide is a prominent candidate for rapid and high-throughput genetic mutation detection, such as SNP analysis.