흰쥐에서 갑상샘 절제 후 재생에 따른 세포 및 세포사이물질의 변화

황영일,정용환,이왕재,황덕호,유영복,김혜숙,장가용 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.4

ICNP를 적용한 학교간호현상 및 특성과 초·중·고등학교의 학교간호현상 비교

김영임,왕명자,양순옥,현혜진,박은옥 韓國學校保健學會 2004 韓國學校保健學會誌 Vol.17 No.1

Purpose : this study purposed to investigate school nursing phenomena in Korea by applying ICNP, the international standard nursing classification system developed by ICN, and to compare school nursing phenomena at elementary, middle and high schools. Method : The subjects of this study are 110 nursing teachers from 82 elementary schools, 16 middle schools, and 12 high schools. In the survey, subjects were asked to score the degree to which phenomena and characteristics of Koran school nursing, identified in previous research, on a 5 point scale. Questionnaires were distributed and recovered by mad and email. The period of data collection was 6 months from July to December 2003. The general characteristics of schools and nursing teachers were represented with frequencies and percentages, the phenomena and characteristics of school nursing with the score of the questions, and the phenomena school nursing by school grade with ANOVA and Duncan's posterior analysis. Results : 1) As for the characteristics of schools according to school nursing phenomena related to human behavior, the mean score of questions on inadequate stress management was highest at 3.24 points followed by the score on inadequate weight control (3.23), inadequate eating habits (3.22), the risk of spine disorders (2.68), inadequate emergency management (2.62), inadequate response to sex -related problems (2.19), and smoking and drug use (1.85). 2) As for the characteristics of schools according to school nursing phenomena related to human function, the mean score of questions on oral health management was highest at 3.11 points followed by the score on the risk of digestive system disorder (2.87), improper eyesight management (2.81), the risk of respiratory system disorders (2.75), lack of sexual identity (2.52), and inadequate contagious disease control (2.12). 3) As for the mean score according to school nursing phenomena related to environment, the score of the risk of accidents in classroom was highest as 2.68 points followed by the score of the risk of accidents around the school (2.66), maladjustment to school (2.62), the risk of accidents outside the classroom (2.43), inadequate learning environment (1.83), the risk of exposure to socially and physically harmful environment factors (La), and inadequate waste disposal (1.77). 4) This study tested the m n scores of questions corresponding to each school nursing phenomenon in order to see if there is a difference in the school nursing phenomenon among elementary, middle and high schools, and performed Duncan's posterior comparison for school nursing phenomena. A significant difference was found at p<.l. According to the results, school nursing phenomena found to be significantly different among elementary, middle and high schools was smoking and drug use (p<.05), which appeared more problematic in high school than in elementary school. phenomena such as inadequate eating habits, inadequate weight control, inadequate response to sex-related problems and inadequate waste disposal were also found to be statistically different at p<.l; however, according to the result of Duncan s posterior comparison, no difference was found among groups in improper eating habits and improper response to sex-related problems, and a significant difference was found between middle and high schools in inadequate weight control and inadequate waste disposal. Conclusion: Based on the results of this study, it is necessary to plan school health projects focused on the characteristic school nursing phenomena that had high scores and to develop and execute school healthe projects in accordance with the characteristics of elementary, middle and high schools. Considering that the scores of school nursing phenomena related to human behavior are high, it is necessary to introduce school health promotion projects in a systematic way.

치료 불응성 Still's병에서 자가 조혈모세포 채집시 사용된 G-CSF에 의한 급성 악화 1예

이재웅,박성현,왕준광,오호석,최정혜,배상철,이영열,김인순,최일영,안명주 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.2

치료 불응성 자가면역질환에서 고용량 항암화학요법 및 자가 조혈모세포이식술이 시행되고 있으나 조혈모세포 가동화 및 이식술 시 사용되는 G-CSF에 의해 오히려 기존의 자가면역질환의 증세가 악화되었다는 보고가 있다. 저자들은 만성적으로 재발되는 Still's병 환자에서 조혈모세포 가동화를 위해 사용한 G-CSF에 의해 자가면역질환이 급성 악화된 1예를 경험하였기에 보고하는 바이다. High-dose immunosuppressive therapy with autologous stem cell transplantation is an increasingly used treatment for severe refractory autoimmune disorder including multiple sclerosis, rheumatoid arthritis, juvenile chronic arthritis, systemic lupus erythematosus. Although the optimal method of collecting stem cell is not determined, G-CSF-based mobilization is generally considered safe. However, worsening of disease status was reported in autoimmune disease undergoing mobilization with G-CSF. We report a 24-year-old male with refractory Still's disease who developed acute disease flare after administration of G-CSF for stem cell mobilization.

유아의 행동문제와 유아교육기관 적응력, 또래관계, 자아탄력성 및 마음이론 간의 관계분석

조유진(Yu-Jin Cho),김민아(Min-Ah Kim),김성현(Seong-Hyun Kim),김채연(Chay-youn Kim),이영민(Young-Min Lee),김순희(Soon-Hee Kim),김진희(Jin-Hee Kim),김보라(Bo-ra Kim),왕혜원(Hye-Won Wang) 중앙대학교 한국교육문제연구소 2015 한국교육문제연구 Vol.33 No.2

본 연구의 목적은 유아의 행동문제와 유아교육기관 적응력, 또래관계, 자아탄력성, 및 마음이론 간 의 관계를 분석하고 유아의 행동문제에 영향을 미치는 예측변인을 밝히는데 있다. 본 연구의 연구대상 은 유치원에 재원중인 만 5세 유아 153명으로 평균 월령은 66.05개월 이였다. ‘K-CBCL 1.5-5’와 유 아교육기관 적응력 검사도구(PAQ)’, ‘또래관계 검사도구(PIPPS)’, ‘자아탄력성 검사도구(DECA)’, ‘ 마 음이론 검사도구를 사용하여 연구자료를 수집하였으며 수집된 자료는 Pearson 적률상관계수와 단계적 중다회귀를 통해 검증하였다. 연구결과, 유아의 내재화, 외현화 행동문제는 또래관계에 유의미한 정적 상관이 있는 것으로 나타났으며, 자아탄력성, 유아교육기관 적응력, 마음이론에 유의미한 부적상관이 있는 것으로 나타났다. 유아의 내재화 행동문제에 있어서는 유아교육기관 적응력이 유의미한 부적 예 측변인으로 나타났으며, 유아의 외현화 행동문제에 있어서는 자아탄력성, 또래관계, 마음이론이 유의미 한 부적 예측변인으로 나타났다. This study investigated the relationships among young children’s problem behavior and peer relation, ego-resilience, preschool adaptation, theory of mind. Subjects used in this study were 153 young children whose mean age of 66.05 months, and who were attended in eight kindergartens. Research instruments used in collecting data were ‘Child Behavior Check List preschool 1.5-5’, ‘Penn Interactive Peer Play Scale(PIPPS)’, The Devereux Early Childhood Assessment(DECA), ‘The Preschool Adjustment Questionnaire(PAQ)', and lastly, ’Theory of Mind’. The collected data were analyzed through SPSS 18.0 program. Pearson’s correlation and Stepwise Multi-Regression Analysis were used to analyze the data. The results of this study were as follows: First, there were positve correlations between young children’s problem behaviors and peer relationship. Second, there were negative correlations between young children’s problem behaviors and ego-resilience, preschool adaptation and theory of mind. Third, preschool adaptation were found to be predictable variables affecting young children’s internalizing problem behavior and peer relation, ego-resilience, and theory of mind were found to be predictable variables affecting young children’s externalizing problem behavior.

Poster : [68] Comparison of Gender Differences in Young Children`s Language, Motor Ability, and Self-Esteem

( Hye Won Wang ),( Myeung Ow ),( Dong Jin Shin ),( Yeonhee Na ),( So Young Seo ) 한국유아교육학회 2014 정기학술대회 논문집 Vol.2014 No.1

Improved Detection of Mycobacterium leprae by One-tube Nested Polymerase Chain Reaction

Hye-Young Wang,Joo-Hwan Whang,Jong-Pill Kim,Jang-Eun Cho,Hyeeun Bang,Hyeyoung Lee,Sang-Nae Cho 대한의생명과학회 2007 Biomedical Science Letters Vol.13 No.4

One-tube nested polymerase chain reaction (PCR) was evaluated for its efficacy in detecting Mycobacterium leprae in biopsy samples from leprosy patients. Primers were derived from the M. leprae-specific element (RLEP) sequences which yield a 230 bp fragment. The specificity and the sensitivity of the one-tube nested PCR were compared with those of single PCR for detecting M. leprae. The results showed that the one-tube nested PCR was about 100 times more sensitive than that of the single indicating the one-tube nested primer sets developed in this study can be an effective screening tool for the detection of M. leprae in clinical diagnostic laboratories.

Use of hTERT and HPV E6/E7 mRNA RT-qPCR TaqMan Assays in Combination for Diagnosing High-Grade Cervical Lesions and Malignant Tumors

Wang, Hye-Young,Park, Sunyoung,Kim, Sunghyun,Lee, Dongsup,Kim, Geehyuk,Kim, Yeun,Park, Kwang Hwa,Lee, Hyeyoung American Society for Clinical Pathology 2015 American journal of clinical pathology Vol.143 No.3

<P>Objectives: Human papillomavirus (HPV) is a major cause of cervical cancer, which is the second most common cancer in women. HPV E6 initiates degradation of cellular tumor suppressor protein p53, induces human telomerase reverse transcriptase (hTERT) activity, and then leads to progressive cervical carcinogenesis. Methods: In this study, the CervicGen HPV RT-qDX assay (Optipharm, Osong, Republic of Korea), which detects 16 HPV high-risk subtypes (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, and 69), and the CervicGen hTERT RT-qDX assay (Optipharm) were evaluated using 545 ThinPrep (Hologic, Bedford, MO Papanicolaou samples. Results: The positivity for the HPV E6/E7 messenger RNA (mRNA) assay was 94.4%, 95.2%, 82.4%, 46.5%, 25.0%, and 1.1% in squamous cell carcinomas, high-grade squamous intraepithelial lesions (HSILs), atypical squamous cells-cannot exclude HSIL, low-grade squamous intraepithelial lesions, atypical squamous cells of undetermined significance, and normal cytology samples, respectively. Five cervical intraepithelial neoplasia grade 2+ samples were not detected by the HPV E6/E7 mRNA assay, but they exhibited positive signals in the hTERT mRNA assay. Notably, the hTERT mRNA expression level was increased in high-grade cervical lesions but was very low in all 288 normal samples. Conclusions: These data suggest that the combination of HPV E6/E7 and hTERT mRNA expression levels could be used in a complementary manner in diagnosing high-grade cervical lesions and malignant tumors and might be useful as a predictive marker in monitoring low-grade cervical lesions.</P>

Multiplex Real-Time PCR Assay for Rapid Detection of Methicillin-Resistant Staphylococci Directly from Positive Blood Cultures

Wang, Hye-young,Kim, Sunghyun,Kim, Jungho,Park, Soon-Deok,Uh, Young,Lee, Hyeyoung American Society for Microbiology 2014 Journal of clinical microbiology Vol.52 No.6

<P>Methicillin-resistant <I>Staphylococcus aureus</I> (MRSA) is the most prevalent cause of bloodstream infections (BSIs) and is recognized as a major nosocomial pathogen. This study aimed to evaluate a newly designed multiplex real-time PCR assay capable of the simultaneous detection of <I>mecA</I>, <I>S. aureus</I>, and coagulase-negative staphylococci (CoNS) in blood culture specimens. The Real-MRSA and Real-MRCoNS multiplex real-time PCR assays (M&D, Republic of Korea) use the TaqMan probes 16S rRNA for <I>Staphylococcus</I> spp., the <I>nuc</I> gene for <I>S. aureus</I>, and the <I>mecA</I> gene for methicillin resistance. The detection limit of the multiplex real-time PCR assay was 10<SUP>3</SUP> CFU/ml per PCR for each gene target. The multiplex real-time PCR assay was evaluated using 118 clinical isolates from various specimen types and a total of 350 positive blood cultures from a continuous monitoring blood culture system. The results obtained with the multiplex real-time PCR assay for the three targets were in agreement with those of conventional identification and susceptibility testing methods except for one organism. Of 350 positive bottle cultures, the sensitivities of the multiplex real-time PCR kit were 100% (166/166 cultures), 97.2% (35/36 cultures), and 99.2% (117/118 cultures) for the 16S rRNA, <I>nuc</I>, and <I>mecA</I> genes, respectively, and the specificities for all three targets were 100%. The Real-MRSA and Real-MRCoNS multiplex real-time PCR assays are very useful for the rapid accurate diagnosis of staphylococcal BSIs. In addition, the Real-MRSA and Real-MRCoNS multiplex real-time PCR assays could have an important impact on the choice of appropriate antimicrobial therapy, based on detection of the <I>mecA</I> gene.</P>

Evaluation of MolecuTech Real MTB-ID for MTB/NTM Detection Using Direct Specimens

Wang, Hye-young,Jin, Hyunwoo,Bang, Hyeeun,Choi, Yeon-Im,Park, Eun-mi,Koh, Won-jung,Lee, Hyeyoung The Korean Society of Clinical Microbiology (KAMJE 2011 Annals of clinical microbiology Vol.14 No.3

Performance of PCR-reverse blot hybridization assay for detection of rifampicin-resistant Mycobacterium leprae.

Wang, Hye-Young,Kim, Hyunjung,Kim, Yeun,Bang, Hyeeun,Kim, Jong-Pill,Hwang, Joo Hwan,Cho, Sang-Nae,Kim, Tae Ue,Lee, Hyeyoung Microbiological Society of Korea 2015 The journal of microbiology Vol.53 No.10

<P>Drug resistance in Mycobacterium leprae is a significant problem in countries where leprosy is endemic. A sensitive, specific, and high-throughput reverse blot hybridization assay (REBA) for the detection of genotypic resistance to rifampicin (RIF) was designed and evaluated. It has been shown that resistance to RIF in M. leprae involves mutations in the rpoB gene encoding the -subunit of the RNA polymerase. The PCR-REBA simultaneously detects both 6 wild-type regions and 5 different mutations (507AGC, 513GTG, 516TAT, 531ATG, and 531TTC) including the most prevalent mutations at positions 507 and 531. Thirty-one clinical isolates provided by Korea Institute of Hansen-s Disease were analyzed by PCR-REBA with RIF resistance of rpoB gene. As a result, missense mutations at codons 507 AGC and 531ATG with 2-nucleotide substitutions were found in one sample, and a missense mutation at codon 516 TAT and ??WT6 (deletion of 530-534) was found in another sample. These cases were confirmed by DNA sequence analysis. This rapid, simple, and highly sensitive assay provides a practical alternative to sequencing for genotypic evaluation of RIF resistance in M. leprae.</P>