Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

차아염소산나트륨 용액과 고압증기멸균이 근관치료용 니켈-타이타늄 파일의 주기적 피로 파절에 미치는 영향

조혜영,정일영,이찬영,김의성 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.1

The purpose of this study was to determine the effect of sodium hypochlorite and steam autoclaving on the cyclic fatigue of nickel-titanium endodontic files. Two types of files with a .06 taper and #30 were used, K3^(ⓡ) (SybronEndo, Glendora, California, USA) and Hero642^(ⓡ)(Micro-Mega, Besancon, France). The files were divided into 6 experimental groups containing 10 files each group depending the soaking time in 6% sodium hypochlorite solution and number of cycles of steam autoclave. After sterilization, a cyclic fatigue test was performed on each file, and the fracture time was recorded in seconds. The control group underwent the cyclic fatigue test only. After the test, the surface characteristics of the files were observed using scanning electron microscopy (SEM). All groups containing the Hero 642^(ⓡ) files showed a similar cyclic fatigue fracture time. However, the cyclic fatigue fracture time with the K3^(ⓡ) files was significantly shorter in groups which were treated with sodium hypochlorite than in the control group (P < 0.05). SEM revealed both Hero642^(ⓡ) and K3^(ⓡ) files to have significant corrosion on the file surface in groups treated with sodium hypochlorite, compared with the sharp and regular blades of the control group. K3^(ⓡ) files showed more corrosion than the Hero642^(ⓡ) files. Bluntness of the blades of the K3^(ⓡ) file was observed in groups treated with steam autoclave. Although there was no obvious destruction on the surface of steam autoclaved Hero642^(ⓡ) files, slight bluntness was observed. Sterilizing with a steam autoclave is much less destructive to K3^(ⓡ) files than sodium hypochlorite. The longer time exposed to sodium hypochlohte, the more destructive pattern was shown on the blades of the files. Therefore, when using sodium hypochlorite solution, the exposure time should be as short as possible in order to prevent corrosion and increase the cyclic fatigue fracture time. 본 연구에서는 차아염소산나트륨 용액 및 고압증기멸균이 근관 치료용 Ni-Ti 파일의 주기적 피로 파절에 미치는 영향을 알아보고자 하였다. 파일 첨단 크기가 #30이고 .06 taper를 가지는 Hero 642^(ⓡ)와 K3^(ⓡ)파일 70개를 10개씩 7개의 군으로 나누었으며, 6% 차아염소산나트륨 용액에 15분 (N15군), 30분 (N30군),고압증기멸균은 121 ℃의 온도, 0.12 ㎫의 압력에서 20 분간 시행하는 것을 일 회로 하여 5회 (H5군), 10회 (H10), 고압증기멸균 5회 후 차아염소산나트륨 용액 15분 (H5N15),고압증기멸균 10회 후 차아염소산나트륨 용액 30분 (H10N30) 처리 후 주기적 피로 파절 실험을 수행하여 파일이 파절될 때까지의 시간을 초 단위로 측정하였다. 대조군은 전처리를 하지 않고 주기적 피로 파절을 시행하였다. 95%의 신뢰도로 one-way analysis of variance를 이용하여 통계 처리하였으며 , 다중 비교는 Tukey방법으로 분석하였다. 파일의 표면을 주사전자현미경으로 관찰하였다. 주기적 피로 파절에 소요된 시간은 Hero 642^(ⓡ)는 각 군별로 유의할 만한 차이가 없었으며, K3^(ⓡ)는 N15, N30, H5N15군에서 대조군에 비해 짧은 시간이 소요된 것으로 나타났다 (p < 0.05). 주사전자 현미경에서 관찰했을 때 Hero 642^(ⓡ)와 K3^(ⓡ) 모두 N15, N30 군에서 파일의 표면과 날이 부식이 되어 대조군에서 관찰된 일정방향으로 배열된 정형화된 예리한 빗살무늬가 부분적으로 파괴된 형태가 관찰되었으며 ,N15군에 비해 N30군에서 파일의 형태가 파괴된 양상이 뚜렷했으며 ,Hero 642^(ⓡ)보다 K3^(ⓡ)가 심하게 부식된 양상이었다. H5군과 H10군에서는 K3^(ⓡ)파일은 날 부분이 무디어진 양상을 보였고, Hero 642^(ⓡ)는 형태 파괴가 뚜렷하지 않았으나 H10군에서 표면의 빗살무늬가 약간 무디어진 양상을 보였다. H5N15군과 H10N30군에서는 그다지 뚜렷한 형태변화는 없었다. 본 연구 결과 K3^(ⓡ)파일을 사용 시에는 가능한 한 차아염소산나트륨에 적은 시간 노출 시키는 것이 파일의 부식으로 인한 형태의 손상을 방지 할 수 있고, 주기적 피로 파절에 걸리는 시간도 감소시킬 수 있을 것이다.

분당 신도시 개발 이후 용인시의 공간구조 변화에 대한 연구

조지혜,김영욱,박영기 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

The purpose of this study was to analyze quantitatively the transformation of spatial structure in the near city of a newtown after the development of it The result is that the development of a newtown had an effect on the trans formation of spatial structure in the near city. The analysis shows that Yongin city's spatial configuration with near cities became strong. And its northwest was more integrated forward Bundang new city than old urban core of it. As a result, it shows a decline of the old urban core.

Gα^(h)-단백질에 의해 활성화되는 Phospholipase C 이소효소의 확인

조강호,박권식,채규영,한기정,김형석,김형일,윤혜영,권년수,이희성,백광진 중앙대학교 의과대학 의과학연구소 2000 中央醫大誌 Vol.25 No.4

복강경을 이용한 부인과 수술의 임상적 고찰

조혜진,정효영,정혁 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives : To assess the advantages, disadvantages, appilcability, and complications of laparoscopy in gynecologic disease. Materials and Method: A retrospective study which includes 591 patients who underwent laparoscopic surgery from January, 1994 to February, 2005 for gynecologic diseases was done. Results: The patient's ages ranged from 19 to 65 years and their mean age was 40years. The indications of laparoscopy were myoma in 240 cases (40.6%), ovarian tumor, ectopic pregnancy, pelvic adhesion, primary infertility, secondary infertility, endometriosis, pelvic inflammatory disease, hemorrhagic corpus luteum and tubal obstruction in decreasing order. The operations performed were laparoscopic-assisted vaginal hysterectomy in 247 cases (41.8%), diagnostic laparoscopy, oophorectomy, salpingo-oophorectorny, ovarian cystectomy, pelvic adhesiolysis, salpingectomy, ovarian wedge resection and fulgarization in order. Operation times were from 10 to 240 minutes (mean 64 minutes). Postoperative hospital stays were from 0 day to 15 days (mean 3.9 days). There were 11 surgical complications including 1 trocar site hematoma, 11 incision site infection, 2 postoperative ileus and 1 patient underwent reoperation because of delayed ureteral fistula. Conclusion: We conclude that operative laparoscopy is useful in gynecologic diseases but need development of laparoscopic equipments, research of operative method, extension of operative indications, and skilled laparoscopic surgeons.

개에서 관찰된 모기질세포종의 증례보고

조성환,손화영,김혜성,최종윤,류시윤,박배근,윤원기,홍성혁,김덕환 충남대학교 수의과대학 동물의과학연구소 2002 動物醫科學硏究誌 Vol.10 No.-

Pilomatricoma was histopathologically diagnosed in a 2.5-year-old male Alaskan malamute. The patient was referred to Chungnam National University, Veterinary Medical Teaching Hospital with solitary, firm and well-circumscribed mass at shoulder. Grossly, the mass was dome shaped to tumor like and 10 x 8 x 5㎝ in size. Some lesions had gritty or bony consistency and was chalky when sectioned Histopathologic feature included mumerous clusters of tightly arranged, basaloid cells with evenly distributed chromatin and ghost cells were characterized by a central unstained zone. The ghost cells often become calcified and osseous metaplasia was also present.

유아 대상 창의성 학습지 분석

조영미,서경혜 이화여자대학교 사범대학 교육과학연구소 2003 교육과학연구 Vol.34 No.3

본 연구의 목적은 유아용 검사도구에 나타난 성역할 고정관념을 분석하므로써 양성평등한 검사도구 개발을 위한 기초 자료를 제공하는 것이다. 분석대상은 1993-2003년까지 간행된 국내논문에서 사용된 유아용 검사도구 28개이다. 연구결과 첫째, 유아용 검사도구는 남˙녀에게 같이 사용되는 혼용이 분리용보다 많았다. 둘째, 유아용 검사도구에 등장하는 인물은 유아의 경우 남아가 여아보다 많았고 성인 등장인물은 여성이 남성보다 많이 나타났다. 셋째, 유아의 성에 따른 활동 유형은 유치원활동과 일상생활에서 여아와 남아의 차이가 비슷하게 나타났다. 성역할 고정관념이 없는 검사도구를 개발하는 측면의 시사점이 제시되어 있다. The present study examined to what extent, if any, children's workbooks promoted the development of creativity. The analysis of children's workbooks focused on the activities in the workbooks, especially the content of activity and the performance and representation children were expected to do in the activity. Five children's workbooks were selected and a total of 193 activities in the workbooks was analyzed. The results of analysis showed that the children's workbooks were limited in many ways for promoting the development of creativity. The content of the activities in the workbooks was largely about spatial relations. The performance and representation children were expected to do in the activities was also limited to following the given examples or choosing the response, In short, the children's workbooks were unlikely to promote the development of creativity, In conclusion, this study offered several suggestions for designing children's workbooks to promote the creativity.

MBTI 성격유형에 따른 청소년의 또래 우정의 질 및 교사 친밀도

조혜영,성영혜 숙명여자대학교 아동연구센터 2002 兒童硏究 Vol.15 No.2

The purpose of this study is to investigate how is the difference at the quality of friendship of age and the intimacy degree of student-teacher according to the teenagers' sex distinction, and also, how is the difference at the quality of friendship of age and the intimacy degree of teacher according to the functional, dispositional, and sixteen nature types by MBTI(Myers-Briggs Type Indicator) test. Moreover, it is to help teachers and advisers at school which teenagers spend a lot of time, at consultation office, and at other institutions recognize the difference according to the sex distinction and the nature type, and also to help them improve the relationship between teenagers and teachers. The subject of this study were 560 students ( boys:308, girls:252) of academic high school located in Seoul and Busan. As a study tool, the MBTI Questionnaire was used to inspect the nature type of teenagers, the Friendship Quality Questionnaire:FQQ which was made by Parker and Asher was used to measure the friendship of age, and the questionnaire for the inspection of intimacy degree of student-teacher was used. For the collected data, the SAS pc program was conducted and according to the study problems, X2-test and the one-way ANOVA were carried out. To see the general characteristics, the frequency and the percentage were used and to see the quality of friendship of age and the intimacy degree of teacher, the mean and standard deviation were used. And then, for the post-verification, the independent t-test was also carried out. To see the quality of friendship of age according to the nature type of teenagers and to see the intimacy degree of teacher, the mean and the standard deviation were used, and then, the Duncan verification was conducted for the post verification. To summarize the results according to the study problems are as follows; First, there was a meaningful difference at the quality of friendship of age according to the sex distinction of teenagers (It I = 8.19, p<.001). That is, girls recognized more highly the friendship of age than boys, and in the intimacy degree of teacher, there was no difference between boys and girls. Second, there was no difference at the quality of friendship of age according to the functional nature type of teenagers, and also, there was no difference in the intimacy degree of teacher. Third, at the quality of friendship of age according to the teenagers' nature, there appeared some differences between the SP(Sensible Perceiving)type and the NT(iNtuitional Thinking)type(F=2.97, P<.05). And, in the intimacy degree of teacher, there appeared some differences between the SJ(Sensible Judging)type, the NT(iNtuitional Thinking)type, and the NF(iNtuitional Feeling)type and the SP(Sensible Perceiving)type(F=2.83, p<.05). Finally, at 16 Types according the MBTI test, there were some differences in the ESFJ group and the ISTP group recognizing the friendship of age (F=3.85, p<.001), and there was no difference in the intimacy degree of teacher.

교정용 미니 임플랜트 고정원과 SWA en masse sliding retraction 시 전치부 치축 조절 요인에 관한 유한요소해석

정혜심,성상진,문윤식,조영수,임승민 대한치과교정학회 2006 대한치과교정학회지 Vol.36 No.5

교정용 미니 임플랜트 고정원을 이용한 교정 치료가 보편화되며, SWA와 이를 이용한 en masse sliding retraction은 임상에서 흔히 사용하는 치료법이 되었다. 그러나 고정원을 성공적으로 보존하려는 노력에 비해, 발치 공간 폐쇄 시 전치부 치축 조절에 관여하는 요인에 대한 보고는 아직까지 부족한 실정이다. 본 연구에서는 제1소구치를 제거한 상악 치아와 치주 인대 그리고 치조골에 대한 3차원 유한요소 기준모델을 제작하였고, 제1대구치와 제2소구치 사이 주호선 10 mm 상방에 식립된 교정용 미니 임플랜트를 고정원으로 사용할 경우, 측절치-견치 사이의 견인 훅의 높이를 변화시키며 후상방 견인력을 가하거나, 주호선에 보상 만곡을 부여하는 것이 전치부 치축 조절에 어떤 영향을 미치는지 시뮬레이션 하였다. 또한 전치부 치축이 설측 경사된 모델을 같은 실험 조건으로 시뮬레이션 하여 발치 공간 폐쇄 시 설측 경사된 전치부 치축을 유지하거나 개선할 수 있는 요인을 검토하였고, 다음과 같은 연구 결과를 얻었다. 2 mm 높이의 견인 훅에 대하여 후상방으로 견인력을 가할 경우 발생하는 함입력으로 인하여 전치부 설측 경사가 더 감소되지는 않았다. 견인 훅의 높이가 5 mm인 경우 후상방 견인력을 가하면, 측절치의 치관 순측 및 치근 설측 이동이 일어나고, 견치의 비조절성 후방 경사 이동이 심화되었다. 4 mm의 보상 만곡은 측절치의 치관 순측 및 치근 설측 이동을 일으키고, 견치의 비조절성 후방 경사 이동을 감소시켰다. 또한 전치부가 설측 경사된 모델을 기준모델과 같은 실험 조건으로 시뮬레이션 한 경우 치근면의 응력 분포와 25000배 확대된 그래프 상에서의 치아 이동 양상은 매우 유사하였다. 이상의 결과는 미니 임플랜트-SWA sliding 생역학을 구사 시 견인 훅의 위치와 와이어 상의 보상 만곡의 유무에 의해 전치부의 치축 조절이 달라지며 실제 임상에서 가이드라인으로 활용될 수 있을 것이다. Objective: With development of the skeletal anchorage system, orthodontic mini-implant (OMI) assisted en masse sliding retraction has become part of general orthodontic treatment. But compared to the emphasis on successful anchorage preparation, the control of anterior teeth axis has not been emphasized enough. Methods: A 3-D finite element Base model of maxillary dental arch and a Lingual tipping model with lingually inclined anterior teeth were constructed. To evaluate factors influencing the axis of anterior teeth when OMI was used as anchorage, models were simulated with 2 mm or 5 mm retraction hooks and/or by the addition of 4 mm of compensating curve (CC) on the main archwire. The stress distribution on the roots and a 25000 times enlarged axis graph were evaluated. Results: Intrusive component of retraction force directed postero-superiorly from the 2 mm height hook did not reduce the lingual tipping of anterior teeth. When hook height was increased to 5 mm, lateral incisor showed crown-labial and root-lingual torque and uncontrolled tipping of the canine was increased. 4 mm of CC added to the main archwire also induced crown-labial and root-lingual torque of the lateral incisor but uncontrolled tipping of the canine was decreased. Lingual tipping model showed very similar results compared with the Base model. Conclusion: The results of this study showed that height of the hook and compensating curve on the main archwire can influence the axis of anterior teeth. These data can be used as guidelines for clinical application.

학생 평가 방식이 학업성취 수준에 미치는 영향 분석

김영식,양민석,조혜승,이호준 이화여자대학교 교육과학연구소 2017 교육과학연구 Vol.48 No.1

In this study, we examined the effect of assessment types on students’ academic achievement. The differential effect of assessment types on students’ achievement according to the degree of teachers’ class improvement activities was also analyzed. For these purposes, we utilized data from SELS and applied the fixed effects model and multiple imputation. The results indicate the assessment types had no statistically significant effect on students’ academic performance. When combined with the improvement in teaching, however, the performance assessment shows statistically significant results for students’ academic achievement. Based on these results, we firstly suggest it would be groundless to associate the decline in academic performance with performance evaluation method. Secondly, we propose significant prerequisites for teachers to further develop their instruction, and for the government and individual schools to provide teachers with greater administrative and financial support.