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김혜련 외 중앙대학교 의과대학 의학연구소 2006 中央醫大誌 Vol.31 No.4
The role of cytogenetics in determining the biologic basis of hematologic malignancy is now widely recognized. By identifying acquired chromosome aberrations, and providing precise chromosomal location of breakpoints in hematologic malignancy associated translocations and inversions, cytogenetics aided in cloning of many genes whose activation or fusion with other genes contributes to the neoplastic process. We reviewed retrospectively chromosomal studies, cytogenetic analyses by high resolution banding technique and clinical records of 246 peripheral blood and 556 bone marrow cases between 1995 and 2006. Among 556 bone marrow cases, 109 cases (19.6%) had clonal chromosomal abnormalities, 30 cases (27.5%) are AML, 20 cases (18.4%) are CML and 16 cases (14.7%) showed MDS. Twenty (83.3%) of the 24 patients with CML had philadelphia chromosome. Among 246 peripheral blood cases, 49 cases (19.9%) had clonal chromosomal abnormalities, 20 cases (34.5%) are Klinfelter syndrome, 12 cases (20.7%) are Down syndrome and 4 cases (6.9%) showed Turner syndrome. In conclusion, cytogenetic analysis should be performed in all patients with hematologic malignancy. Therefore there is a constant need for large prospective studies correlating karyotype with selected molecular genetic markers, gene expression profiles, immunophenotype, other biologic parameters and clinical outcome.
브로콜리에 있어서 突然變異劑 處理前과 處理後의 꽃봉오리 및 小花梗 培養
李洙聖,金辰熺,方惠蓮 中央大學校 食糧資源硏究所 1993 食糧資源硏究所 論文集 Vol.5 No.1
브로콜리의 꽃봉오리와 小花梗 培養法을 확립하였다. MS의 基本培地에 BA와 β-NAA를 각각 1㎎/l, 그리고 sucrose가 3% 첨가된 固體培地에 이들 조직을 치상하면 약 30일 후에 80%이상의 조직에서 그리고 組織當 평균 적어도 5개 이상의 순을 얻을 수 있다. 이 순은 植物生長調整劑가 첨가되지 않은 MS 基本培地에 sucrose가 2% 첨가된 固體培地로 옮겨주면 뿌리가 分化하여 완전한 식물체로 발달하였다. 한편 이들 조직을 突然變異劑 EMS(ethyle methane sulphonate)의 10mM또는 20M 용액에 각지 60분 또는 30분간 침지 처리한 후 배양함으로서 꽃봉오리에서는 무처리에 비해 약 50%의 순을 얻을 수 있다. 그리고 NMU(n-methyle-n-nitroso-urea)의 0.2mM용액에 20분정도 침지처리한 후 배양하면 꽃봉오리에서는 置床組織數의 약 60%에서, 그리고 小花梗에서는 약 30%에서 식물체를 얻을 수 있었다. In general, more than 80% of flower bud and pedicels of broccoli produced shoots when they were cultured on the solid medium of Murashinge and Skoog(MS) supplemented with 3% of sucrose and 1㎎/l of each of benzyl adenine(BA) and 2-naphthalene acetic acid(NAA). Shoots subcultured on the MS basic medium containing only 2% of sucrose developed enough roots to transplant for acclimatization. On the other hand, doses of chemical mutagenes, ethyl methane sulphonate(EMS) and n-methyl n-nitroso urea(MNU), were investigated for treatment to the flower bud and the pedicel. Flower buds treated with EMS either 10mM for 60 minutes or 20mM for 30 minutes produced shoots about 50% in the number compared to the non-treated one. In the case of MNU treatment, about 60% and 20% of the cultured buds and pedicels, respectively, produced shoots in the dose of 0.2mM for 20 minutes.
Gallbladder Cancer Presenting as an Isolated Large Lung Mass
Kim, Hye-Ryoun,Cheon, Gi Jeong,Koh, Jae Soo,Yang, Sung Hyun,Kim, Cheol Hyeon,Lee, Jae Cheol Lippincott Williams Wilkins, Inc. 2009 Clinical nuclear medicine Vol.34 No.11
A large lung mass was incidentally found in a 68-year-old man. The chest computed tomography (CT) showed a lobulated mass in the right lower lung without enlargement of mediastinal lymph nodes. Well-differentiated adenocarcinoma was revealed by pathologic examination. Unexpectedly, a hypermetabolic lesion of standardized uptake value (SUV) 8.2 was noted in the gallbladder by fluoro-deoxy-glucose (18F-FDG-PET)/CT during staging work-up for lung cancer. A markedly thickened fundus of the gallbladder was shown on abdominal CT. Subsequent immunostainings for TTF-1 and CK-7 were negative suggesting the lung mass would not be primarily originating from the lung. Abdominal surgery was performed to remove the gallbladder mass, which was followed by right lower lobectomy of lung. An isolated lung metastasis from gallbladder cancer was confirmed although it is extremely rare.
Kim, Seo Yun,Myung, Jae Kyung,Kim, Hye-Ryoun,Na, Im Il,Koh, Jae Soo,Baek, Hee Jong,Kim, Cheol Hyeon The Korean Academy of Tuberculosis and Respiratory 2019 Tuberculosis and Respiratory Diseases Vol.82 No.1
Background: Epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancers have emerged as key predictive biomarkers in EGFR tyrosine kinase inhibitor (TKI) treatment. However, a few patients with wild-type EGFR also respond to EGFR TKIs. This study investigated the factors predicting successful EGFR TKI treatment in lung adenocarcinoma patients with wild-type EGFR. Methods: We examined 66 patients diagnosed with lung adenocarcinoma carrying wide-type EGFR who were treated with EGFR TKIs. The EGFR gene copy number was assessed by silver in situ hybridization (SISH). We evaluated the clinical factors and EGFR gene copy numbers that are associated with a favorable clinical response to EGFR TKIs. Results: The objective response rate was 12.1%, while the disease control rate was 40.9%. EGFR SISH analysis was feasible in 23 cases. Twelve patients tested EGFR SISH-positive, and 11 were EGFR SISH-negative, with no significant difference in tumor response and survival between EGFR SISH-positive and -negative patients. The overall median progression-free survival (PFS) and overall survival (OS) of 66 patients were 2.1 months and 9.7 months, respectively. Female sex and Eastern Cooperative Oncology Group (ECOG) performance status (PS) of 0-1 were independent predictors of PFS. ECOG PS 0-1 and a low tumor burden of extrathoracic metastasis were independent predictors of good OS. Conclusion: Factors such as good PS, female sex, and low tumor burden may predict favorable outcomes following EGFR TKI therapy in patients with EGFR wild-type lung adenocarcinoma. However, EGFR gene copy number was not predictive of survival.
( Hye Ryoun Jang ),( Ajin Jo ),( Ji Hyeon Park ),( Jung Eun Lee ),( Woo Seong Huh ),( Dae Joong Kim ),( Ha Young Oh ),( Yoon Goo Kim ) 대한신장학회 2011 Kidney Research and Clinical Practice Vol.30 No.6
Purpose: Urinary angiotensinogen (AGT) has been reported as an important marker reflecting the activity of intrarenal renin-angiotensin system (RAS) in chronic glomerulonephritis patients. We investigated urinary AGT excretion and intrarenal AGT expression in patients with minimal change disease (MCD). Methods: In 20 patients with biopsy-proven MCD, urinary and plasma AGT was measured using a sandwich ELISA and intrarenal AGT expression was measured with immunohistochemistry. Urine samples from normal healthy volunteers and patients with biopsy-proven thin basement membrane disease (TBM) were used as control groups. Results: MCD patients showed a wide range of natural logarithm of the urinary AGT/creatinine [ln (urinary AGT/Cr)] and the ln (urinary AGT/Cr) was higher in MCD patients compared with normal controls and TBM controls (normal control vs. TBM vs. MCD, 1.2±0.25 vs. 0.9±0.34 vs. 3.2±0.40). Intrarenal AGT expression was diverse in MCD patients (intrarenal AGT, arbitrary unit, 27.39-78.52 in TBM, 0.00-145.80 in MCD). Ln (urinary AGT/Cr) did not show a direct correlation with intrarenal AGT expression, plasma AGT, or urinary protein/creatinine ratio. Conclusion: Urinary AGT excretion and intrarenal AGT expression are enhanced in some MCD patients, suggesting that intrarenal RAS is activated in these patients.
Kim, Seok Jin,Lee, Ga Yeon,Jang, Hye Ryoun,Choi, Jin-Oh,Kim, Jung Sun,Kim, Hee-Jin,Lee, Soo-Youn,Min, Ju-Hong,Jeon, Eun-Seok,Kim, Kihyun Informa UK Ltd. 2013 Amyloid Vol.20 No.4
<P>Autologous stem cell transplantation (ASCT) has improved the prognosis of selected patients with immunoglobulin amyloid light-chain (AL) amyloidosis. However, there exist little data regarding Asian patients receiving ASCT. We retrospectively analyzed 24 patients who were treated with ASCT for AL amyloidosis between 2007 and 2012. The median age at the time of ASCT was 56 years (range: 40-62 years), and in 63% of patients, the AL amyloidosis involved two or more organs. All patients except one received induction treatment such as cyclophosphamide, dexamethasone or thalidomide before ASCT, but only approximately half of these patients showed a hematologic response. After ASCT, the overall hematologic response was increased to 92% (22/24), which translated into increased organ response in 58% of patients (14/24). There was no transplantation-related mortality (0%) even though cardiac amyloidosis patients were included in our series. In conclusion, our results of ASCT in patients with AL amyloidosis were comparable to that of Western countries in terms of response and survival outcomes. Therefore, ASCT is an effective and feasible treatment approach for Asian patients with AL amyloidosis.</P>