The effects of human mesenchymal stem cells treatment on the alopecia areata mice models

( Hye Ree Park ),( Song Hee Park ),( Yu Jin Lee ),( Kyung Jae Lee ),( Jung Eun Kim ),( Hoon Kang ) 대한피부과학회 2021 대한피부과학회 학술발표대회집 Vol.73 No.1

A case of pityriasis rosea-like eruption induced by amoxicillin

( Hye Ree Park ),( Ji Hee Jung ),( Dong Geon Lee ),( Hoon Kang ),( Jung Eun Kim ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

Pityriasis rosea (PR) is a relatively common dermatologic disorder with an acute self-limiting papulosquamous lesion. It is characterized by distinctive skin eruptions and minimal constitutional symptoms. Drug induced PR like eruption can be an important cause of recurrent PR and tend to occur in older generation. Herein, we report a case of PR like eruption induced by amoxicillin. A 77-year-old male patient with hypertension presented with intermittently pruritic papules and patches on the trunk and lower extremities for 2 months. Physical examination revealed multiple, variable-sized, erythematous papules and patches which were symmetrically distributed mainly on the flank and thighs. He had been started on oral amoxicillin before the itchy rash developed and the lesion recurred after taking amoxicillin again. Histopathologic examination revealed hyperkeratosis, irregular acanthosis of epidermis and perivascular, interstitial infiltration of lymphocytes and eosinophils on the upper dermis. Based on these findings, the diagnosis of drug induced PR like eruption was made. Medications reported to be implicated PR like eruptions include antibiotics like penicillin and pristinamycin, antidepressants, biological agents, metals and vaccines. Dechallenge test of the suspected drug is helpful to confirm the relation of drug and development of PR.

A case of malignant proliferating trichilemmal tumor on the scalp treated with radiotherapy and MTX-intralesional injection

( Hye Ree Park ),( Dong Geon Lee ),( Kyung Jae Lee ),( Jung Eun Kim ),( Hoon Kang ) 대한피부과학회 2021 대한피부과학회 학술발표대회집 Vol.72 No.2

Case-control study: the effects of synthetic ceramide on dandruff, erythema, sebum secretion and water loss of scalp

( Hye Ree Park ),( Dong Geon Lee ),( Ji Hee Jung ),( Jung Eun Kim ),( Hoon Kang ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: Ceramide is important in barrier function of epidermis. It can reduce epidermal water loss and inhibit producing dandruff. Ceramide is an essential component in protecting hair and can be used as a cosmetic ingredient to improve seborrheic dermatitis and subjective symptoms like pruritus. Objectives: We aimed to evaluate the efficacy of synthetic ceramide on dandruff, erythema, sebum secretion and water loss. Methods: Total of 20 subjects, 10 were randomly assigned to ceramide group and other 10 were assigned to controlled group. The subjects had received the evaluation of scalp before the trial and after using shampoo 15ml once a day for 4 weeks. Scores were assessed about dandruff and scalp erythema. Sebum was collected using sebumeter and sebum secretion was recorded at 4 parts (frontal, vertex, both temporal area). Water content of scalp was measured using corneometer at same sites. Results: After 4 weeks, 8 patients had favorable outcomes with ceramide shampoo in dandruff compared with 4 patients in control. And 4 patients had favorable outcomes with ceramide shampoo in erythema compared with 3 patients in control. Sebum secretion increased in ceramide group and decreased in control, but not statistically significant. Water content showed a tendency to increase in ceramide group, whereas water content significantly decreased in control. Conclusion: These results indicate that synthetic ceramide is effective in improving dandruff, erythema and reducing water loss.

Inflammasome-mediated inflammation by malassezia in human keratinocytes: a comparative analysis with different strains

( Hye Ree Park ),( Ji Hee Jung ),( Dong Geon Lee ),( Kyung Jae Lee ),( Jee Hye Oh ),( Eu Jin Lee ),( Song Hee Park ),( Yang Won Lee ),( Seongju Lee ),( Hoon Kang ),( Jung Eun Kim ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1

Background: Malassezia species are associated with several common dermatologic conditions including pityriasis versicolor, seborrheic dermatitis, folliculitis, and atopic dermatitis and dandruff. However, its causal role remains to be established. Objectives: We intended to explore the role of inflammasome activation in human keratinocytes in response to three different Malassezia species. Methods: We compared the different activation patterns of inflammasomes and the expression of proinflammatory cytokines and antimicrobial peptides by three different Malassezia species―M. restricta, M. globosa, and M. sympodialis―in human keratinocytes. Results: We found that different Malassezia species, especially M. restricta, and M. globosa could induce nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin-domain-containing protein (NLRP)3- apoptosis-associated speck-like protein containing CARD (ASC) inflammasome activation and subsequent interleukin (IL)-1β secretion in human keratinocytes. Malassezia species variably induced thymic stromal lymphopoietin, β -defensin 2, and LL-37. IL-6 mRNA and IL-22 protein significantly increased in the M. sympodialis-treated group, and CCL17 and CCL27 mRNA were increased in response to M. globosa-treated keratinocytes. Conclusion: Our data show that various species of Malassezia promote variable inflammatory responses in keratinocytes by activating NLRP3 inflammasomes, pro-inflammatory cytokines and chemokines, and antimicrobial peptides.

한국형 비만 관련 삶의 질 측정도구 개발

박혜순,선우성,오상우,이가영,김병성,한지혜,김선미,이혜리,유병연,이근미,서영성,남윤덕,박용우,신호철,이정권 대한비만학회 2003 The Korean journal of obesity Vol.13 No.4

연구배경: 비만 환자는 신체적, 정신적, 기능적 건강 문제를 가지게 되며 삶의 질이 낮다고 알려져 있다. 삶의 질을 측정하기 위해 외국의 도구를 번역하여 사용하는 경우 문화적인 차이와 그 나라 고유 언어표현을 충분히 반영하지 못하는 등의 문제가 있다. 이에 한국인 비만 환자에서 삶의 질을 간편하게 측정할 수 있는 도구를 개발하여 신뢰도와 타당도를 검증하였다. 방법: 한국형 건강관련 삶의 질 측정 도구를 개발한 경험이 있는 전문의와 협의하여 비만 관련 삶의 질 정의 및 영역을 설정하였다. 이를 토대로 180명의 비만 환자로부터 비만 관련 증상을 채집하였다. 채집된 증상을 같은 의미를 나타내는 증상끼리 묶고 각 영역으로 범주화하였으며 문항에 적합하게 수정하였다. 이에 대해 사전 설문 조사한 후 각 영역별로 내적일치도를 검토하여 최종 문항을 선택하였다. 7개 병원의 비만 클리닉과 가정의학과 외래로 내원한 환자 240명을 대상으로 타당도를 분석하였고, 이 중 4주 이내에 검사-재검사법을 실시한 38명의 자료에 대하여 신뢰도를 검증하였다. 결과: 총 215개의 증상이 채집되었으며 범주화를 통하여 정신사회적 건강, 신체적 건강, 직장 및 가사업무, 일상 생활, 성관계, 음식 관련 등 6개의 삶의 질 영역, 15개의 문항으로 정리되었다. 측정 도구의 내적 일치도가 비교적 높게 나타났고 검사-재검사법에 의한 상관 계수도 유의하게 높게 나타났으며, 문항-영역간, 문항-총점간 상관성도 모든 문항에서 유의성을 나타내었다. 요인 분석 결과는 3개의 요인으로 묶여 비만 관련 삶의 질을 측정하기에 타당도가 있는 것으로 나타났다. 삶의 질 각 영역별 점수는 모든 영역에서 비만군 및 복부 비만군과 대조군 사이에 유의한 차이가 있는 것으로 나타났다. 결론: 6 영역, 15 문항으로 구성된 한국형 비만 관련 삶의 질 측정도구를 개발하였으며 이 도구는 신뢰도와 타당도가 높은 것으로 나타났다. 한국인 비만 환자에게 간편하게 사용할 수 있을 것으로 여겨지며 추후 지속적인 개정 작업이 필요하다. Objective: Obesity researchers have a growing interest in measuring the impact of weight and weight reduction on quality of life. The KOQOL (Korean version of Obesity-related QOL scale) was the self-report instrument specifically developed to assess the effect of obesity on quality of life reflecting Korean's own language expression and culture. This report was conducted to establish verification of the reliability and validity of a 15-item version of the KOQOL. Methods: Symptom descriptions related with obesity were collected from 180 obese people based on definition and domains of ‘obesity related quality of life'. The collected results were categorized into each domain and edited to be used as questions. The expert panels established final 6 domains, 15 items, and item option responses. Reliability was tested by internal consistency method and 2 weeks test-retest method. Validity test was performed by factor analysis and clinical validity. Results: A total of 215 symptom descriptions were collected and categorized into 15 items of 6 domains including phychosocial health, physical health, work, routine life, sexual life, and diet distress. Items were corrected for more precise meaning, concise sentence, and proper expression. A high degree of internal consistency was observed for each of domain except routine life. Two weeks test-retest reliability correlation coefficients scores were highly significant in all domains. Item-to-scale and item-to total score correlations were highly significant for all items. A principal components analysis identified 3 factors with strong support for the adequacy of the scale structure. Significant differences in KOQOL scale and total scores were found among groups differing in body mass index or abdominal circumference, supporting the utility of the KOQOL. Conclusion: The KOQOL composed of 15 items of 6 domains (phychosocial health, physical health, work, routine life, sexual life, and diet distress) was demonstrated as an excellent tool for measuring of obesity related quality of life. The KOQOL appears to be a reliable and clinically valid brief measure of quality of life in Korean obese persons.

Inhibition of Adenylyl Cyclase Type 5 Prevents <small>l</small>-DOPA-Induced Dyskinesia in an Animal Model of Parkinson's Disease

Park, Hye-Yeon,Kang, Young-Mi,Kang, Young,Park, Tae-Shin,Ryu, Young-Kyoung,Hwang, Jung-Hwan,Kim, Yong-Hoon,Chung, Bong-Hyun,Nam, Ki-Hoan,Kim, Mee-Ree,Lee, Chul-Ho,Han, Pyung-Lim,Kim, Kyoung-Shim Society for Neuroscience 2014 The Journal of neuroscience Vol.34 No.35

<P>The dopamine precursor <SMALL>l</SMALL>-3,4-dihydroxyphenylalanine (<SMALL>l</SMALL>-DOPA) is widely used as a therapeutic choice for the treatment of patients with Parkinson's disease. However, the long-term use of <SMALL>l</SMALL>-DOPA leads to the development of debilitating involuntary movements, called <SMALL>l</SMALL>-DOPA-induced dyskinesia (LID). The cAMP/protein kinase A (PKA) signaling in the striatum is known to play a role in LID. However, from among the nine known adenylyl cyclases (ACs) present in the striatum, the AC that mediates LID remains unknown. To address this issue, we prepared an animal model with unilateral 6-hydroxydopamine lesions in the substantia nigra in wild-type and AC5-knock-out (KO) mice, and examined behavioral responses to short-term or long-term treatment with <SMALL>l</SMALL>-DOPA. Compared with the behavioral responses of wild-type mice, LID was profoundly reduced in AC5-KO mice. The behavioral protection of long-term treatment with <SMALL>l</SMALL>-DOPA in AC5-KO mice was preceded by a decrease in the phosphorylation levels of PKA substrates ERK (extracellular signal-regulated kinase) 1/2, MSK1 (mitogen- and stress-activated protein kinase 1), and histone H3, levels of which were all increased in the lesioned striatum of wild-type mice. Consistently, FosB/ΔFosB expression, which was induced by long-term <SMALL>l</SMALL>-DOPA treatment in the lesioned striatum, was also decreased in AC5-KO mice. Moreover, suppression of AC5 in the dorsal striatum with lentivirus-shRNA-AC5 was sufficient to attenuate LID, suggesting that the AC5-regulated signaling cascade in the striatum mediates LID. These results identify the AC5/cAMP system in the dorsal striatum as a therapeutic target for the treatment of LID in patients with Parkinson's disease.</P>

A case of recurrent merkel cell carcinoma

( Hye Ree Park ),( Dong Geon Lee ),( Ji Hee Jung ),( Jung Eun Kim ),( Hoon Kang ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.1

Merkel cell carcinoma (MCC) is a rare and aggressive form of skin cancer that appears as a solitary erythematous to pinkish nodule. The most of MCCs are located on the head and neck, followed by the extremities that are associated with sun exposure. It has a propensity for frequent lymph node and distant metastasis as well as aggressive local invasion. Herein, we report a case of MCC that recurred after surgery. A 67-year-old female patient with hypertension and cataract presented with a nodule on the cheek for 2 months. Physical examination revealed a solitary, 0.8x0.6 cm-sized, dome-shaped, pink to reddish nodule on the left cheek. The lesion was asymptomatic but increased in size. Histopathologic finding showed dense infiltration of basophilic small round cells composing tumor that were positive for cytokeratin 20 and negative for cytokeratin 7 and leukocyte common antigen in immunohistochemistry. Based on the clinical and histological findings, she was diagnosed as MCC and underwent surgical excision. The patient revisited our department 7 months after surgery and presented with a solitary, 0.5x0.4 cm-sized, skin-colored, palpable subdermal nodule on the left cheek for a week. Skin biopsy of the same site lesion was performed and the diagnosis of recurrent MCC was made. We did Moh’s microscopic excision with rotational flap coverage and referred her to the department of radiation oncology for adjuvant radiotherapy.

한국형 비만 관련 삶의 질 측정도구 개발

박혜순,선우성,오상우,이가영,김병성,한지혜,김선미,이혜리,유병원,이근미,서영성,남윤덕,박용우,신호철,이정권 대한비만학회 2003 The Korean journal of obesity Vol.12 No.4

The safety and efficacy evaluation of 0.33% brimonidine gel for Korean patients with rosacea

( Hye Ree Park ),( Ji Hee Jung ),( Young Jun Woo ),( Jung Eun Kim ),( Hoon Kang ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: Erythema in rosacea patients results from dysregulation of cutaneous vasomotor responses. A 0.33% brimonidine gel (Mirvaso^®; Galderma SA, Lausane, Switzerland) is a highly selective α2 adrenergic agonist approved for the treatment of erythema in rosacea. However, there are rare data on the safety and efficacy of repeated application of brimonidine gel for Koreans Objectives: The aim of this study is to evaluate the safety and efficacy of topical 0.33% brimonidine gel for Korean patients with rosacea Methods: 80 Korean patients with rosacea aged over 18 are educated to apply brimonidine gel once a day for 8 weeks. To evaluate safety, adverse events (AEs) including rash, pruritus, and tingling were checked and clinician’s erythema assessment (CEA), patient self-assessment (PSA), clinical global photographs, colorimetric test and subject satisfaction questionnaire (SSQ) were used to evaluate efficacy Results: 12.5% of subjects experienced local AEs that tended to decrease as they visited. There was no severe AE. CEA, PSA, clinical global photographs, and colorimetric scores were significantly improved compared to visit 1 and most of the respondents answered to SSQ were positive about topical 0.33% brimonidine Conclusion: We confirmed that topical 0.33% brimonidine is definitely effective on the erythema of rosacea. Despite the low possibility of severe AEs, clinicians have to pay attention to local AEs of topical 0.33% brimonidine.