NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.

성폭력 피해여성의 경험에 관한 연구

정연강,권혜진,지순주,남선영,김경희 韓國學校保健學會 1996 韓國學校保健學會誌 Vol.9 No.1

This studies designed to work out a theoretical framework on the experience of sexual abuse from the perspective of grounded theory in an effort to provide more practical and efficient nursing intervention for female victims. The subcategories identified were "sexual abuse", "threatening", "absent mindness", "embarrassment", "horripilation", "dizziness", "wondrousness", "filthiness", "sexual curiousity", "violence level", "victim's age", "neighbors response", "victims personality", "common experience", "sexual abuse information", "family relations", "level of familiarity", "hiding", "suppression", "self-torture", "self-protection", 'avoidance", "asking aid", "withdrawal", "hatred", "confusion", "dodging", "remmant", and "pursuing". The 29 subcategories given above were further integrated into 16 categories such as "victimizedness", "being astounded", "filthiness", "degree", "developmental stage", "response pattern", "personality", "rarity", "infromation availability", "family support", "cover-up", "escaping", "informing", "negative internalization", and "positive pursuit of change". The core categories linked to all the other categories turned out to be "being taken aback" and "filthiness" incorporation the relevant subcategories. A total of 23 theoretical hypothesis emerged in the process of analyzing data. 1. the grater sexual curiosity, the weaker the senses of being taken aback and filthiness. 2. The weaker sexual curiosity, the stronger the senses of being taken aback and filthness. 3. The stronger the level of violence, The more violent the senses of being taken aback and filthiness. 4. The lower the level of violence, the weaker the senses of being taken aback and filthiness. 5. The younger the victims, the stronger the senses of being taken aback and filthness. 6. The older the victims, The weaker the senses of being taken aback and filthness. 7. 'Escaping' will transpire regardless of the given circumstances. 8. The weaker the senses of being taken aback and filthness, the more probable 'informing' and 'escaping' transpire. 9. The stronger the senses of being taken aback and filthness, the more probable 'informing' and 'escaping' transpire. 10. The more protective the response from 'informing' and 'escaping' transpire around, the more likely the response to 'being taken aback' and 'filthiness' will be 'informing' and 'escaping'. 11. The more repelling the response from around, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 12. The more open minded the personality of subject, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 13. The more closed the personality of subject, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 14. The more frequent the experience of sexual abuse, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 15. The less frequent the experience of sexual abuse, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 16. The more available information concerning sexual abuse, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 17. The less available information concerning sexual abuse, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 18. The more cohesive the family of the subject, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 19. The less cohesive the family of the subject, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 20. The more familiar the subject is with the abuser, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 21.The less familiar the subjec is with the abuser, the more likely the response to 'being taken aback' and 'filthiness' will be 'covering-up' and 'escaping'. 22. The more likely the response to 'being taken aback' and 'filthines' is 'informing' and 'escaping', the more positive changes the subject will pursue. 23. The more likely the response to 'being taken aback' and 'filthiness' is 'covering-up' and 'escaping', the more negative changes the subject will pursue. The following four hypothneses were conformed in the process of data analysis. 1) In case the level of violence is strong but 'being taken aback' and 'filthiness' in weak because of strong sexual curiosity and also if information concerning sexual abuse is not readily available and the frequency is low, negative internationalization marked by 'covering-up' and 'escaping' will take place despite the fact the subject is open-minded, the family is cohesive and the abuser is unfamiliar. 2) In case the level of violence is weak but 'being taken aback' and 'filthiness' is weak combined wit weak sexual curiosty and also if information concerning sexual abuse is readily available and the response from around is protective and the frequency is high, the subject will pursue positive changes to 'being taken aback' and 'filthiness' , further aided by the fact that the subject is open-minded, the family is cohesive and the abuser is unfamilar. 3) In case the level of violence is strong but 'being taken aback' and 'filthiness' is strong because of weak sexual curiosity and also if information concerning sexual abuse is reading available and the response from around is readily available and the response from around is protecvice and the frequency is low, the subject will persue positive changes marked by 'informing' and 'escaping' despit the fact that the family cohesion is weak and the abuser is familiar. 4) In case the level of violence is strong but 'being taken aback' and 'filthiness' is strong because of weak sexual curiosity and also if information concerning sexual abuse is not readily available and the response from around is respelling and the frequnecy is low negative internalization like 'covering-up' and 'escaping' will take place, further aggravated by the fact that the subject's personality is closed, family cohension is weak, and subject is familiar. On the basis of the above finding, it is recommended that nursing intervention should focus on promoting the milieu conductive to the victims pursuing positive changes along with the adequate aids from protection facilities as well as from the people around them.

림프절 생검으로 진단된 복강내 간질섬유형성 소원형 세포 종양 1예 보고

주명진,이광민,이혜경,정동규 대한병리학회 1995 Journal of Pathology and Translational Medicine Vol.29 No.5

대학생의 의류쇼핑성향과 청바지 구매 시 점포선택

박혜정,신은주,정혜영 한국의류학회 2004 한국의류학회지 Vol.28 No.5

The purposes of this study were to classify shopper types based on clothing shopping orientations and to identify the differences in store selection criteria and demographic characteristics by shopper types. The questionnaire was administered to female and male undergraduate and graduate students living in Seoul. Of 330 returned questionnaires, 319 were used in the statistical analysis which were factor analysis, cluster analysis, x²-test, and One-way ANOVA. The results of this study were as follows: 1) Clothing shopping orientations had six factors: recreational shopping, name conscious shopping, economic shopping, fashion oriented shopping, convenience shopping, and individualistic shopping. Cluster analysis identified that clothing shopping orientations had four groups: recreational cluster, individualistic cluster, demanding shopper cluster, and convenient brand conscious shopper cluster. 2) Clothing shopping orientations were significantly different in relation to the demographic characteristics such as gender, major field of study, expenditure on clothing, pocket money, and family income level. 3) Store selection criteria had five factors: service quality, physical store environment, sales personnel, shopping convenience, and other attractions. 4) There were significant differences in physical store environment, shopping convenience, and other attractions according to the shopper clusters.

환기정도에 따른 수술실용 신발 종류가 수술실 오염에 미치는 영향 : How to Choose Suitable Shoes?

남경동,정혜선,박영신,원진희,주미자,성화신,이지혜,이병희,조경숙,배재춘 한국의료QA학회 2000 한국의료질향상학회지 Vol.7 No.1

Background : Various types of protective footwear have been used to minimize bacterial contamination in operating rooms. In recent years, debate has arisen concerning the need for use of such protective footwear. This study was designed to provide useful data about choosing shoes most suitable for the surgical environment. Methods : Between November, 1999 and January, 2000, we performed this experimental study by comparing effect of three types of shoes (i.e., disposable shoescover, operating room-restricted shoes, and ordinary shoes) on bacterial contamination of operating rooms equipped with two different ventilation systems (i.e., high air-change, low air-change) respectively. Data were collected during two-hour sham operations in which subjects and their activities were strictly standardized. Bacterial flora were sampled from the study area floor and air colony-counts were measured. Results : In experiments involving high air-change ventilation system, there was a significant difference of floor contamination between three types of shoes, but no difference of air contamination. Under low air-change system, there was a significant difference of both floor and air contamination between three types of shoes. Conclusion : The results show that protective footwear would be unnecessary in the operating room with high air-change ventilation system, but it is important to choose suitable shoes carefully under low air-change system. Therefore, the use of outdoor shoes can be considered under high air-change system, but it would seem sensible to apply their first use in less bloody operations at the day surgery center or out-patient department to prevent transfer of body fluid into the outside environment.

일 도농복합지역 저소득층 노인의 건강문제 분석 : 보건소 방문간호사업 대상자 중심으로

고일선,,이태화,이경자,이정렬,임미혜,천의영,주윤미,이계철 노인간호학회 2005 노인간호학회지 Vol.7 No.2

Purpose: The purpose of this study was to analyze the health problems of low-income aged with chronic illness living in urban-rural composite area. Method: The sample consisted of 440 aged who were receiving home care services from a public health center. MDS-HC was applied to analyze the health problems of the aged. Data were collected through a face- to-face interview by six trained interviewers from June 28 to July 15, 2004. Result: Subjects had average number of 8 health problems in both urban and rural area. Lack of preventive health care measure, pain, and visual function were the most frequent health problem. The rural aged had more pain, bowel management problems, compared to the urban aged having more urinary incontinence and indwelling catheter. There were many health problems related to falls and pressure ulcers with middle-old aged in urban, and old-old aged in rural area. Conclusion: The results of this study showed strategies for care intervention of low-income elderly to put in practice. Therefore, tailored-service for each subject should be provided.

자궁경부 상피내종양의 진단 및 치료에서 환상 투열요법(LLETZ)의 임상적 의의

김승철,이경순,김영주,정혜원,안정자 梨花女子大學校 醫科大學 醫科學硏究所 1994 EMJ (Ewha medical journal) Vol.17 No.4

The technique of large loop excision of the transformation zone(LLETZ) as an alternative to destructive/ablative methods of treatment of CIN(cervical intraepihelial neoplasia) such as cryotherapy, laser vaporization and cone biopsy was assessed. In 63 women with suspected CIN attending the colposcopy clinic, the histologic diagnoses made from excision biopsies taken by LLETZ were compaired with those from colposcopically directed punch biopsies. This microinvasive tumors were found by LLETZ and the diagnosis made by LLETZ was worse than that made by punch biopsy in 17 cases(26.9%). That is to say, LLETZ improves the accuracy of diagnosis of CIN. And in offers a cheap and accurate alternative to laser vaporization or cone biopsy, and also combines the benifits of the gold standard cone biopsy with that of local ablative metholds. LLETZ is considered as a fairly new and good method of treatment of CIN, and the olng-term complications or effects should by yet fully evaluated in future.

Personality Types of Korean Reproductive Women with Polycystic Ovary Syndrome

( Shin Hye Chung ),( Je Yeon Lee ),( Kyung Ah Jeong ),( Hye Sun Chung ),( Eun Ju Lee ),( Ju Young Lee ),( Hye Won Chung ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.11

Objective The aim of this study was to compare personality types between polycystic ovary syndrome (PCOS) patients and women with normal menstrual cycle. Methods A total of 2,311 Korean women aged 15-39 was recruited. After initial screening, 131 women were excluded who had polarity index under 45. The study subjects were divided into PCOS patients (n=833) and women with normal menstrual cycle (n=1,347). Selfadministered questionnaire surveys, somatometry, blood test and transvaginal or transrectal ultrasound test were performed. By using Myers-Briggs Types Indicator (MBTI) personality types including characteristic index, function and temperament were also assessed. Results There was no signifi cant difference in the frequency of personality types between the patient and control groups. Among 16 MBTI subtypes, the extraversion-sensing-thinking-judgement type was the most common in both groups, 25.69% in PCOS patients and 24.51% in control group. In analysis of the judgement-perception index, the p
erceiving type was predominant in PCOS patients (30.73%) than control group (25.09%) with a statistical signifi cance ( P=0.013). The average testosterone level in women with the extroversion type was 53.43 ± 20.13 ng/dL, which was signifi cantly higher than women with the introversion type (51.58 ± 18.60 ng/dL). Conclusion Higher frequency of the perception type is typical feature in PCOS patients. Hyperandrogenism is closely related to the extroversion type of personality.

A Study on the Deperm Protocols Considering Demagnetizing Field of a Ferromagnetic Material

Hye Sun Ju,Hyuk Won,Hyun Ju Chung,Gwan Soo Park 한국자기학회 2014 Journal of Magnetics Vol.19 No.1

Magnetic materials with large coercive force and high squareness ratio are currently developing to meet an industrial demand. Since a ferromagnetic material has hysteresis characteristics, it is hard to demagnetize a ferromagnetic material precisely. In this paper, we describe deperm processes and conduct an analysis of residual magnetization of ferromagnetic material using the Preisach modeling with a two-dimensional finite elements method (FEM). From the results, it was shown that an exponential decrement form of deperm protocol is more efficient than a linear decrement form because of the demagnetizing field in the ferromagnetic material.

Comparison of the Validity of Three Biomarkers for Gastric Cancer Screening: Carcinoembryonic Antigen, Pepsinogens, and High Sensitive C-reactive Protein

Chung, Hye Won,Kim, Ju Won,Lee, Jong-han,Song, Si Young,Chung, Jae Bock,Kwon, Oh Hun,Lim, Jong-Baeck Lippincott WilliamsWilkins, Inc. 2009 JOURNAL OF CLINICAL GASTROENTEROLOGY Vol.43 No.1

PURPOSE: To identify a desirable serum marker for screening tools for gastric cancer, we evaluated the validity of 3 biomarkers, namely, carcinoembryonic antigen (CEA), pepsinogens (PGs), and high sensitive C-reactive protein (hsCRP). METHODS: We estimated the mean serum levels of CEA, PGs, and hsCRP and compared the sensitivity and specificity of these 3 biomarkers in 378 subjects who were classified into 7 groups: normal, chronic atrophic gastritis, intestinal metaplasia, adenoma, early gastric cancer (EGC), advanced gastric cancer (AGC) without metastasis, and AGC with metastasis (M1). RESULTS: There were no significant differences among the normal, high-risk (chronic atrophic gastritis, intestinal metaplasia, and adenoma), and EGC groups for CEA and hsCRP. However, the levels of CEA were relatively higher in the AGC group with intestinal-type cancer (P<0.01). Likewise, hsCRP was relatively higher in the AGC group with diffuse-type cancer (P<0.01). For the PG I/II ratio, there was no significant difference among the normal, high-risk, and cancer groups, including EGC (P<0.01). In addition, there was a negative correlation with grades (&ggr;s=−0.480, P<0.01). However, the PG I/II ratio was relatively less effective in diffuse-type cancer compared with intestinal-type cancer. The combination of serum hsCRP and the PG I/II ratio had a higher sensitivity (77%) than did the PG I/II ratio alone (61%) in diffuse-type cancers. CONCLUSIONS: The combination of serum hsCRP and PG I/II ratio would be helpful as a screening tool for gastric cancer in high incidence populations and may help to select high-risk subjects in need of further specific invasive screening tools such as endoscopy.