동맥혈 채혈후 시간 경과 및 온도 변화가 가스분압 및 PH 에 미치는 영향에 관한 연구

김동수,이승환,김건식,강화자,신광일,여민구 대한마취과학회 1989 Korean Journal of Anesthesiology Vol.22 No.6

Blood gas samples are highly susceptible to preanalytic error due to improper methods of obtaining or handling the sample prior to delivery to the laboratory. The errors in the measurement of blood gas analysis are currently derived from the exposure of sample to atmosphere, effects of anticoagulant itself, temperature difference between the measuring electrode and drawn blood and the delay in running the sample. To study the effects of the delay in measuring the sample and the temperature difference between the measuring electrode and drawn blood on values of blood gases and pH, we analyzed the arterial sampling from the 24 patients who were taking elective surgery or on his/her recovery period with indwelling arterial catheter. The plastic sampling syringes were kept at 4。C (refrigerator) or 22。-24。C (room temperature) and analyzed at regular intervals (1, 10, 30, 60,120 min) for 120 minutes. The following results were obtained: 1) When the arterial blood drawn from the anesthetized patients were stored 4。C, partial pressure of oxygen (PaO₂) decreased significantly after 20 min, whereas those stored at room temperature decreased significantly after 10 min. 2) When the arterial blood drawn from the recovery patients were stored at 4。C, PaO₂ did not decrease significantly through the experimental period of 120 min. Although those stored at room temperature did not decrease significantly through the period of 120 min. 3) Partial pressure of carbon dioxide in the arterial blood (PaCO₂,) drawn from the anesthetized patients increased significantly by 120 min. at 4。C, whereas those at room temperature increased significantly after 20 min. 4) PaCO₂, of the recovery patients increased signigicantly by 120 min. at 4。C, whereas those at room temperature increased significantly after 30 min. 5) pH of the arterial blood drawn from either anesthetized or recovery patients decreased significantly by 120 min. at 4。C, whereas those at room temperature decreased significantly after 60 min. 6) No significant changes al oxygen saturation (SaO₂) and content (CaO₂) were noted in either anesthetized or recovery. patients in accordance with time elapsed at 4。C or room temperature. In summary, as the changes of PO₂ in particular higher than physiologic PO₂ and PCO₂ in the arterial blood stored at room temperature are significant in accordance with the delay in measuring, it would be advisable to analyze the sample in a short period of time or to store it in a cool place when the measuring will be delayed.

해도 정보를 이용한 선박의 최적 항로 생성

김민규(Min-Kyu Kim),김종화(Jong-Hwa Kim),양현(Hyun Yang) 한국항해항만학회 2022 한국항해항만학회 학술대회논문집 Vol.2022 No.2

Min-Kyu Kim*․Jong-Hwa Kim**․†최근 자율 운항 선박에 대한 관심이 높아지고 있다. 특히, MUNIN (Maritime Unmanned Navigation through Intelligence in Networks) 프로젝트를 계기로 자율 운항 선박에 대한 개발과 연구가 활발히 진행되고 있다. 또한 국제해사기구 IMO는 자율 운항 선박 시대에대응하기 위해 자율 선박을 MASS (Maritime Autonomous Surface Ship)라 정의하고 선박 자율화 정도에 따라 4단계 등급을 제시하고 있다. 완전한 자율 운항 선박에 대한 요구조건을 만족하기 위해서는 항로 결정과 제어기술이 필수적이다. 본 연구에서는 여러 가지 기술 중 선박의최적경로를 생성하는 기법을 다룬다. 기존에 최적항로를 생성하기 위한 방법으로는   , Dijkstra와 같은 알고리즘들이 주로 사용되었다. 그러나 이와 같은 알고리즘은 섬이나 육지에 대한 충돌 회피는 고려하고 있지만 수심 및 연안 선박에 대한 규정들은 고려하지 않고 있어실제로 적용하기에는 한계점이 있다. 따라서 본 연구에서는 안전을 위해 선박의 선저 여유 수심과, 해도에 규정되어 있는 선박 운항에 대한여러 규정들을 반영하여 최적 항로를 생성하고자 한다. 최적 항로를 생성하기 위한 알고리즘으로는 강화학습 기반의 Q-learning 알고리즘을적용하였다.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

Effects of Red Ginseng on Neonatal Hypoxia-induced Hyperacitivity Phenotype in Rats

Kim, Hee-Jin,Joo, So-Hyun,Choi, In-Ha,Kim, Pitna,Kim, Min-Kyoung,Park, Seung-Hwa,Cheong, Jae-Hoon,Shin, Chan-Young The Korean Society of Ginseng 2010 Journal of Ginseng Research Vol.34 No.1

Attention deficit hyperactivity disorder (ADHD) affects 4-12% of chool-age children worldwide and is characterized by three core symptoms: hyperactivity, inattention, and impulsivity. Although standard pharmacological treatments, such as methylphenidate and atomoxetine, are available, concerns about drug-induced psychological and cardiovascular problems, as well as growth retardation and sleep disturbances, highlight the continuing need for new therapeutic interventions. Using a neonatal hypoxia-induced hyperactivity model in rats, the potential positive role that oral administration of red ginseng extract may have in relation to the hyperactive phenotype was investigated. Hypoxia was induced in 2-day-old male Sprague-Dawley (SD) rat pups by placing them in a nitrogen chamber for 15 min. The neonatal hypoxia-induced rats showed a significant increase in hyperactivity phenotype, such as increased movement duration, movement distance, and rearing frequency, which was determined by monitoring their spontaneous locomotor activity using the Ethovision video tracking system. One week of oral treatment with red ginseng extract decreased the hyperactivity phenotype of the neonatal hypoxia-induced rats and increased the locomotor activity of the control rats. In the neonatal hypoxia-induced rats, expression of the norepinephrine transporter in the forebrain was increased, and red ginseng treatment partially prevented its up-regulation, while increasing its level in the control rats. Taken together, these results suggest that red ginseng extract decreased the neonatal hypoxia-induced hyperactivity phenotype, although it increased locomotor activity in normal animals.

Nonselective Blocking of the Sympathetic Nervous System Decreases Detrusor Overactivity in Spontaneously Hypertensive Rats

Kim, Khae-Hawn,Jin, Long-Hu,Choo, Gwoan-Youb,Lee, Hun-Jae,Choi, Bo-Hwa,Kwak, Jiyeon,Yoon, Sang-Min,Park, Chang-Shin,Lee, Tack Molecular Diversity Preservation International (MD 2012 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.13 No.4

<P>The involuntary dual control systems of the autonomic nervous system (ANS) in the bladder of awake spontaneously hypertensive rats (SHRs) were investigated through simultaneous registrations of intravesical and intraabdominal pressures to observe detrusor overactivity (DO) objectively as a core symptom of an overactive bladder. SHRs (<I>n</I> = 6) showed the features of overactive bladder syndrome during urodynamic study, especially DO during the filling phase. After injection of the nonselective sympathetic blocking agent labetalol, DO disappeared in 3 of 6 SHRs (50%). DO frequency decreased from 0.98 ± 0.22 min<SUP>−1</SUP> to 0.28 ± 0.19 min<SUP>−1</SUP> (<I>p</I> < 0.01), and DO pressure decreased from 3.82 ± 0.57 cm H<SUB>2</SUB>O to 1.90 ± 0.86 cm H<SUB>2</SUB>O (<I>p</I> < 0.05). This suggests that the DO originating from the overactive parasympathetic nervous system is attenuated by the nonselective blocking of the sympathetic nervous system. The detailed mechanism behind this result is still not known, but parasympathetic overactivity seems to require overactive sympathetic nervous system activity in a kind of balance between these two systems. These findings are consistent with recent clinical findings suggesting that patients with idiopathic overactive bladder may have ANS dysfunction, particularly a sympathetic dysfunction. The search for newer and better drugs than the current anticholinergic drugs as the mainstay for overactive bladder will be fueled by our research on these sympathetic mechanisms. Further studies of this principle are required.</P>

Photovoltaic properties of TiO2-based solar cells with sodium ethanesulfonate-modified photoelectrodes

Kim, Sun Jie,Kim, Wook Hyun,Lee, Chang-Hyun,Kim, Hwa-Min,Kang, Cheon Su,Han, Yoon Soo Informa UK (TaylorFrancis) 2017 MOLECULAR CRYSTALS AND LIQUID CRYSTALS - Vol.651 No.1

<P>Effects of sodium ethanesulfonate (SES) adsorbed on photoelectrode surface on the performance of dye-sensitized solar cells (DSSCs) was investigated. SES-modified photoelectrode was prepared by soaking the TiO2 film to an aqueous SES solution for 20 min. The DSSC with SES-modified TiO2 layer showed an increase in open circuit voltage (V-oc), short circuit current (J(sc)) and fill factor (FF), resulting in a power conversion efficiency of 11.20%, compared to that of reference device with bare TiO2 (10.21%). Electrochemical impedance spectroscopy and open circuit voltage decay measurements revealed that the SES modification of TiO2 surface led to a longer electron lifetime by the suppression of the interfacial charge recombination between photo-injected electrons and I-3(-) ions, resulting in an increase in J(sc) and V-oc, compared with those of a reference device without surface modification.</P>

축산분뇨 혐기소화 잔재물의 특성 및 초지 적용성 평가

김승민(Seung Min Kim),정웅기(Woong-gi Jung),성용주(Yong Joo Sung),안희권(Hee-Kwon Ahn),김동성(Dong Sung Kim),윤도현(Do-Hyun Yoon),김동섭(Dong-Seop Kim1),정광화(Hwa Gwang Jung) 한국펄프·종이공학회 2014 펄프.종이기술 Vol.46 No.3

Effects of Red Ginseng on Neonatal Hypoxia-induced Hyperacitivity Phenotype in Rats

Hee Jin Kim,So Hyun Joo,Inha Choi,Pitna Kim,Min Kyoung Kim,Seung Hwa Park,Jae Hoon Cheong,Chan Young Shin 고려인삼학회 2010 Journal of Ginseng Research Vol.34 No.1

Attention deficit hyperactivity disorder (ADHD) affects 4-12% of chool-age children worldwide and is characterized by three core symptoms: hyperactivity, inattention, and impulsivity. Although standard pharmacological treatments, such as methylphenidate and atomoxetine, are available, concerns about drug-induced psychological and cardiovascular problems, as well as growth retardation and sleep disturbances, highlight the continuing need for new therapeutic interventions. Using a neonatal hypoxia-induced hyperactivity model in rats, the potential positive role that oral administration of red ginseng extract may have in relation to the hyperactive phenotype was investigated. Hypoxia was induced in 2-day-old male Sprague-Dawley (SD) rat pups by placing them in a nitrogen chamber for 15 min. The neonatal hypoxia-induced rats showed a significant increase in hyperactivity phenotype, such as increased movement duration, movement distance, and rearing frequency, which was determined by monitoring their spontaneous locomotor activity using the Ethovision video tracking system. One week of oral treatment with red ginseng extract decreased the hyperactivity phenotype of the neonatal hypoxia-induced rats and increased the locomotor activity of the control rats. In the neonatal hypoxia-induced rats, expression of the norepinephrine transporter in the forebrain was increased, and red ginseng treatment partially prevented its up-regulation, while increasing its level in the control rats. Taken together, these results suggest that red ginseng extract decreased the neonatal hypoxia-induced hyperactivity phenotype, although it increased locomotor activity in normal animals.

Genome Wide Expression Profile of Agrimonia pilosa in LPS-stimulated BV-2 Microglial Cells

Sohn, Sung-Hwa,Ko, Eun-Jung,Kim, Sung-Hoon,Kim, Yang-Seok,Shin, Min-Kyu,Hong, Moo-Chang,Bae, Hyun-Su The Korean Society of Toxicogenomics and Toxicopro 2009 Molecular & cellular toxicology Vol.5 No.1

Microglial cells constitute the first line of defense against infection and injury in the brain. This study was conducted to evaluate the protective mechanisms of Agrimonia pilosa (AP) on LPS-induced activation of BV-2 microglial cells. The effects of AP on gene expression profiles in activated BV-2 microglial cells were evaluated using microarray analysis. BV-2 microglial cells were cultured in a 100 mm dish ($1{\times}10^7/mL$) for 24 hr and then pretreated with 1 g/mL AP or left untreated for 30 min. Next, 1 g/mL LPS was added to the samples and the cells were reincubated at $37^{\circ}C$ for 30 min, 3 hr and 6 hr. The gene expression profiles of the BV-2 microglial cells varied depending on the AP. The microarray analysis revealed that MAPK signaling pathway-related genes were down-regulated and IL10 gene was up-regulated in AP-treated BV-2 microglial cells. AP can affect the inflammatory response and MAPK pathway in BV-2 microglial cells.

Genomewide Expression Profile of Forsythia Suspensa on Lipopolysaccaride-induced Activation in Microglial Cells

Sohn, Sung-Hwa,Ko, Eun-Jung,Kim, Yang-Seok,Shin, Min-Kyu,Hong, Moo-Chang,Bae, Hyun-Su The Korean Society of Toxicogenomics and Toxicopro 2008 Molecular & cellular toxicology Vol.4 No.2

Microglia, which is the primary immune effector cells in the central nervous system, constitutes the first line of defense against infection and injury in the brain. The goal of this study was to determine the protective (anti-inflammation) mechanisms of forsythia suspense (FS) on LPS-induced activation of BV-2 microglial cells. The effects of FS on gene expression profiles in activated BV-2 microglial cells were evaluated using microarray analysis. BV-2 microglial cells were cultured in a 100mm dish $(1{\times}10^7/dish)$ for 24hr and then pretreated with $1{\mu}g/mL$ FS or left untreated for 30 min. Next, $1{\mu}g/mL$ LPS was added to the samples and the cells were reincubated at $37^{\circ}C$ for 30 min, 1hr, and 3hr. The gene expression profiles of the BV-2 microglial cells varied depending on the FS. The oligonucleotide microarray analysis revealed that MAPK pathway-related genes such as Mitogen activated protein kinase 1 (Mapk1), RAS protein activator like 2 (Rasal2), and G-protein coupled receptor 12 (Gpr12) and nitric oxide biosynthesis-related genes such as nitric oxide synthase 1 (neuronal) adaptor protein (Nos1ap), and dimethylarginine dimethylaminohydrolase 1 (Ddah1) were down regulated in FS-treated BV-2 microglial cells. FS can affect the MAPK pathway and nitric oxide biosynthesis in BV-2 microglial cells.