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Le, Thi Huong,Nga, Thi Thu Vu,Nguyen, Ngoc Dung,Le, Thi Thanh Xuan,Kim, Bao Giang,Phan, Thi Hai,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Tuan Lam,Hoang, Van Minh,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1
The aim of this paper is to report the rate of current and ever cigarette smoking and explore correlates of current cigarette smoking among adolescents aged 13-15 in Viet Nam. This analysis was derived from GYTS survey, which comprised of 3,430 adolescents aged 13-15, conducted in 2014 in 13 cities and provinces of Viet Nam. We calculated the weighted rates of current and ever cigarette smoking and reported patterns of smoking behavior. We also performed logistic regression to explore correlates of current cigarette smoking behavior. The weighted rate of ever cigarette smoking was 9.5% (95% confidence interval (CI): 8.5 %-10.5%), in which the weighted rate among males (15.4%; 95% CI: 13.6%-17.0%) was higher than that among females (4.2%; 95% CI: 3.3%-5.1%). The weighted rate of current cigarette smoking was relatively low at 2.5% (95%CI: 2.0%-3.0%) with higher weighted rate among males (4.9%; 95% CI: 3.8%-5.9%) compared to the corresponding figure among females (0.2%; 95% CI: 0.0 %-0.5%). Current cigarette smoking was significantly higher among males than females, in students aged 15 versus 13 years old, and in students who had several or all close friends smoking and students with daily observation of smoking at school. For greater smoking reduction outcomes, we recommend that tobacco interventions for adolescents should consider targeting more male students at older ages, establish stricter adherence to school-based banning of cigarette smoking, engage both smoking and nonsmoking adolescents and empower adolescents to resist peer smoking influence as well as changing their norms or beliefs towards smoking benefits.
Characterization of Two Cryptic Plasmids from Levilactobacillus zymae GU240
LE HUONG GIANG,김민재,전혜성,유지연,Kang Yun Ji,Kim Tae Jin,김정환 한국미생물·생명공학회 2022 한국미생물·생명공학회지 Vol.50 No.1
Two small cryptic plasmids, pHG1 and pHG2, were isolated from Levilactobacillus zymae (formerly Lactobacillus zymae) GU240 and characterized. pHG1 is 1,814 bp in size with a GC content of 37.4% and contains two open reading frames. orf1 can potentially encode a protein of 101 amino acids (aa) with 99% identity with the copy number control protein of Lacticaseibacillus paracasei. orf2 can potentially encode a protein of 230 aa with 99% identity with a replication protein from multiple species. Six inverted repeats (IR I-VI) and six direct repeats (DR I-VI) were found in pHG1. pHG2 is 2,864 bp in size, with a GC content of 39.6%. pHG2 has two orfs. orf1 might encode a protein with 99% identity with the TrsL transmembrane protein. orf2 might encode a protein with 99% identity with plasmid recombination proteins from lactic acid bacteria. Both pHG1 and pHG2 may be useful as frames for constructing lactic acid bacteria–Escherichia coli shuttle vectors.
( Huong Giang Le ),( Zhuang Yao ),( Jeong A Kim ),( Se Jin Lee ),( Yu Meng ),( Ji Yeong Park ),( Jeong Hwan Kim ) 한국미생물 · 생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.3
Lactobacillus zymae GU240 was previously isolated from Kimchi, a Korean fermented vegetable, as a strong GABA producer. The strain showed β-galactosidase (β-Gal) activity on MRS agar plates with X-gal. When growth and β-Gal activities of GU240 were measured using MRS (glucose, 2%, w/v) and MRSL (lactose, 2%, w/v) broths, cells were found to grow slowly in MRSL, and the β-Gal activity (36 units at 4 h) was lower than that of cells grown in MRS (94 units at 16 h). The highest OD600 value of the culture in MRS was 1.6 at 24 h at 37℃, whereas that of the culture in MRSL was 0.6 at 16 h. β-Gal activity of the culture in MRS reached the maximum (95.6 u/ml) at 16 h, decreased thereafter, and was not detected at 48 h. β-Gal activity for culture in MRSL reached its highest (36 u/ml) at 4 h and decreased gradually, but some activity (11.05 u/ ml) still remained at 72 h. The structural gene encoding β-Gal in L. zymae GU240 was cloned as a 3.1 kb fragment, and DNA sequencing confirmed the presence of complete lacLM genes. lacLM genes from L. zymae GU240 showed 98-99% homologies in nucleotide sequences with other lacLM genes from L. brevis. Reverse transcription (RT)-PCR confirmed the operon structure of lacLM. The results indicated that L. zymae GU240 might be in the process of losing the ability to grow rapidly on lactose-containing medium, such as milk, due to adaptations to plant environments, including kimchi.
Chemical Constituents of the Morinda tomentosa Leaves and their α-Glucosidase Inhibitory Activity
Vu, Huong Giang,Ninh, Khac Ban,Nguyen, Xuan Nhiem,Phan, Van Kiem,Chau Van, Minh,Hoang, Le Tuan Anh,Pham, Hai Yen,Bui, Huu Tai,Nguyen, Xuan Cuong,Nguyen, Hoai Nam,Kim, Seung Hyun,Kwon, Young-In,Kim, Yo Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.5
Nguyen Le Minh Tri,김지태,Bach Long Giang,T.M. Al Tahtamouni,Pham Thi Huong,이창하,Nguyen Minh Viet,Do Quang Trung 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-
In this study a novel Ag-doped graphitic carbon nitride (g-C3N4) photocatalyst was synthesized and appliedas high efficientmaterial under solarlight towardsemerging antibiotic pollutantin hospital wastewater. Thetetracycline (TC) was chosen as a target pollutant and the content of Ag doping at 3 mmol revealed thehighest photocatalytic degradation efficiency of TC (96.8%) after 120 min under solar light irradiation. Thephotoluminescence and UV–vis analysis confirmed the enhancement of charge separation and transfer inthe graphitic carbon structure after Ag-doping. The removal efficiency of TC using g-C3N4 and Ag-doped g-C3N4 (AgCN) underdark conditions was only 25.6 and 31.8%, respectively. While under solarlight conditions,the removal efficiency of TC increased to 68.3 and 96.8% for g-C3N4 and AgCN, respectively. The reusabilityprocess showed that AgCN displayed extremely high stability after 6 cycles without significant drop inantibiotic degradation efficiency. The application of AgCN was tested for treatment of TC from hospitalwastewater and it showed high removal efficiency of 89.6% within 120 min reaction time. In addition, theintermediatesgeneratedandreductionof total organiccarbon(TOC)duringthephotocatalyticreactionweredetected to support information of possible TC removal mechanism.
VO, Thi Huong Giang,LUONG, Duy Binh,LE, Khoa Huan Korea Distribution Science Association 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.6
With the dramatic increase in mobile usage, more and more businesses see the potential of m-commerce. This study focuses on a subcategory of m-commerce, a mobile shopping application. To understand the purchase intention via m-commerce applications, this study is aimed to identify the main factors that are related to the applications and explore the influence of these factors on consumers' mobile shopping intention. This study uses quantitative research methods and selects Vietnam as its case study. The survey responses of 450 Vietnamese mobile shoppers were analyzed using partial least squares structural equation modeling (PLS-SEM). The results indicated that online reviews, e-service quality, and information quality are significant predictors of behavior intention, and perceived risk negatively influences consumer online purchase intention via the applications. The content enriches the combined research of detailed and possible models with quality dimensions and risk perception. Practitioners such as e-retailers and developers can enhance the quality of applications and determine strategies to reach potential users and maximize revenue. M-commerce providers should pay adequate attention to credible and influential online reviews since mobile shoppers heavily rely on reading reviews before buying a product.
Zhuang Yao,Yu Meng,Huong Giang Le,김정아,김정환 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.4
A Bacillus strain, SJ4, exhibiting strong fibrinolytic activity was isolated from saeu (shrimp, Acetes chinensis) jeotgal, a Korean traditional fermented food and was identified as B. subtilis. The B. subtilis SJ4 strain can grow at a NaCl concentration of up to 15% (w/v). The fibrinolytic activity of B. subtilis SJ4 (152.0 U/ml) cultured in Luria-Bertani (LB) broth for 48 h at 37℃ with aeration was higher than that of B. subtilis SJ4 cultured in TSB (124.5 U/ml) under same culture conditions. The major proteins in the LB culture supernatant of B. subtilis SJ4 were analyzed by SDS-PAGE, which revealed three major bands (23, 25, and 28 kDa). The band (23 kDa) with strong fibrinolytic activity, analyzed on fibrin zymogram, was observed at 60−96 h of cultivation. The aprESJ4 gene encoding the major fibrinolytic enzyme, AprESJ4, was cloned by PCR. The aprESJ4 gene sequence exhibited high similarities with the fibrinolytic gene sequences of other Bacillus species. The amino acid sequence of AprESJ4 exhibited 98.9 and 98.4% similarity with subtilisin NAT and AprE2 of B. subtilis, respectively. Hence, B. subtilis SJ4 can be a potential starter culture for jeotgal products.
Zhuang Yao,Yu Meng,Huong Giang Le,이세진,전혜성,유지연,김현진,김정환 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.11
We have previously characterized AprESJ4, the major fibrinolytic enzyme from Bacillus subtilis SJ4 (Yao et al., 2019). During that study, we observed a 68 kDa protein with fibrinolytic activity. In this study, we cloned the gene (vprSJ4) encoding the 68 kDa protein, a mature Vpr and minor protease secreted by Bacillus species. vprSJ4 encodes a preproenzyme consisting of 810 amino acids (aa) including signal sequence (28 aa) and prosequence (132 aa). The mature enzyme (650 aa) has a predicted molecular weight of 68,467.35. Unlike Vprs from other B. subtilis strains, VprSJ4 has 4 additional amino acids (DEFA) at the C-terminus. vprSJ4 was overexpressed in Escherichia coli. PreproVprSJ4 was localized in inclusion bodies, and subjected to in vitro renaturation and purification by an affinity column. SDS-PAGE and western blot showed that autoprocessing of preproVprSJ4 occurred and 68 kDa and smaller proteins were produced. The optimum pH and temperature of the recombinant VprSJ4 were pH 7.0 and 40oC, respectively. Kinetic parameters of recombinant VprSJ4 were measured by using an artificial substrate, N-succinyl-ala-ala-pro-phe-pnitroanilide. Coexpression of vprSJ4 and aprESJ4 using pHY300PLK increased the fibrinolytic activity a further 117% when compared with aprESJ4 single expression using the same vector in B. subtilis WB600.
Optimization of Spin-Valve Structure NiFe/Cu/NiFe/IrMn for Planar Hall Effect Based Biochips
Bui Dinh Tu,Le Viet Cuong,Tran Quang Hung,Do Thi Huong Giang,Tran Mau Danh,Nguyen Huu Duc,CheolGi Kim IEEE 2009 IEEE transactions on magnetics Vol.45 No.6
<P>This paper deals with the planar Hall effect (PHE) of Ta(5)/NiFe(t<SUB>F</SUB>)/Cu(1.2)/NiFe(t<SUB>P</SUB>)/IrMn(15)/Ta(5) (nm) spin-valve structures. Experimental investigations are performed for 50 mumtimes50 mum junctions with various thicknesses of free layer (t<SUB>F</SUB> = 4, 8, 10, 12, 16, 26 nm) and pinned layer (t<SUB>P</SUB> = 1, 2, 6, 8, 9, 12 nm). The results show that the thicker free layers, the higher PHE signal is observed. In addition, the thicker pinned layers lower PHE signal. The highest PHE sensitivity S of 196 muV/(kA/m) is obtained in the spin-valve configuration with t<SUB>F</SUB> = 26 nm and t<SUB>P</SUB> = 1 nm. The results are discussed in terms of the spin twist as well as to the coherent rotation of the magnetization in the individual ferromagnetic layers. This optimization is rather promising for the spintronic biochip developments.</P>