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Hui-Fen Wu,Suresh Kumar Kailasa,Ja-Yi Yan,Chen-Che Chin,Hsin-Yi Ku 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4
This paper describes the use of single-drop microextraction and micro-volume pipette extraction coupled with capillary electrophoresis for the analysis of five antidepressant drugs (nortriptyline, imipramine, trimeprazine, promethazine, and iminodibenzyl). The five antidepressant drugs are well separated by using micellar electrokinetic capillary chromatography with SDS and organic modifiers. The best extraction was achieved within 10 min and effectively separated within 15 min by using ammonium acetate buffer at 28 kV. This method shows good reproducibility, with RSD values ranging from 4.3 to 8.7%. The LODs values were found to be 0.4 and 1.0 mg mL-1 for SDME-CE and 0.15 and 0.5 mg mL-1 for MVPE-CE. These methods are simple, rapid, sensitive and efficient for the extraction, separation and detection of tricyclic antidepressant drugs.
Lu Hui,Li Fen,Zhu Xiaoming,Tang Jihong,Lyu Baoqian,Wu Shaoying 한국곤충학회 2021 Entomological Research Vol.51 No.9
Spraying insecticides by unmanned aerial vehicle (UAV) is an effective method to prevent and control Spodoptera frugiperda. This study investigated the influences of different UAV types, spray adjuvants, spray times, maize growth periods, and insecticides on S. frugiperda in three cities, Hainan Province, China. The results showed that at least two times are needed to spray insecticides using UAVs when controlling the S. frugiperda. Both UAVs had a substantially higher control effect and lower cost than artificial spray methods. Oil spray adjuvant Refei was more suitable for both UAVs than Tiancheng to control pests. Chlorantraniliprole had the best control effect of up to 90%. The control effects at the seedling stage and small bell stage of S. frugiperda were 94.3% and 91.3%, respectively. Spraying at 7:00 am and 7:00 pm by UAVof T20 had good control effect on S. frugiperda in three cities.
Three homologous genes encoding functional D8-sphingolipid desaturase in Populus tomentosa
Shu-Fen Li,Zan-Min Hu,Guo-Jun Zhang,Ying-Chun Yuan,Cong-Hui Wang,Wu-Jun Gao,Chuan-Liang Deng,Long-Dou Lu 한국유전학회 2014 Genes & Genomics Vol.36 No.3
Δ8-sphingolipid desaturase is characterized by itsability to catalyze desaturation at the C8 position of the longchainbase of sphingolipids in plants. No previous studieshave been conducted on genes encoding Δ8-sphingolipiddesaturases in the woody plant Populus tomentosa. In thisstudy, three genes that encode Δ8-sphingolipid desaturasewere isolated fromP. tomentosa. Among these genes, PtD8Aand PtD8B showed high sequence similarity; whereas PtD8Cexhibited large sequence divergence.RT-PCRresults showedthat PtD8A and PtD8B were expressed in all tissues detected,whereas PtD8C was not expressed in roots. Heterologousexpression in yeast revealed that PtD8A/B/C were functionalΔ8-sphingolipid desaturases, and can catalyze the C18-phytosphingeninedesaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine.However, the conversion rate and ratios ofthe two products differed. Compared with control cells,transgenic yeasts expressing PtD8A/B/C exhibited enhancedaluminum tolerance. Our findings further elucidated thebiochemical functions and evolutionary history ofΔ8-sphingolipid desaturases in plants. Candidate genes forbreeding new poplar germplasm resources with enhancedtolerance ability to aluminium were also provided.
Hemal Weerasinghe,Hui-Fen Wu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.124 No.-
Vanadium dioxide has excellent absorbance in near-infrared (NIR) wavelengths making it a perfect candidatefor photothermal applications based on solar energy. However, up to date, it was mainly applied ina lithium-ion battery. This study is for the second time, to utilize VO2-based nanomaterials as a photothermalmaterial for solar steam generation to purify seawater and wastewater. The study has proposeda straightforward and promising room temperature approach to synthesize a mixture of twovanadium oxide nanosheets with a major fraction of VO2 (B) nanosheets with the major part for VO2. nH2O and a minor fraction of V2O5.nH2O which have demonstrated excellent photothermal capabilityfor water purification. The highest steady state temperature of 87 C was reported by the synthesizednanosheets that were spray coated on cellulose fabric for the solar absorption purpose under one sun illuminationfor water purification. The as-prepared photo absorber was capable of reaching the higheststeady temperature at high speed (within 10 minutes). The synthesized 2D VO2/V2O5.nH2O nanosheetsand cellulose fabric combination of solar steam generator gained a competitive evaporation rate of1.31 kgm-2h1 and a percentage efficiency of 89.7% under one sun illumination. The current approachwas successfully applied to three real-world samples to obtain clean or drinking water. All three samplesshowed excellent improvements in their water quality compared with their initial states in a single distillation. Two samples can even reach the quality of World Health Organization (WHO)-recommendedstandards for drinking water. The current approach has opened a new platform for the utilization ofVO2-based nanosheets for obtaining clean water from seawater and wastewater by solar energyutilization.
Du, Ze-Peng,Wu, Bing-Li,Xie, Jian-Jun,Lin, Xuan-Hao,Qiu, Xiao-Yang,Zhan, Xiao-Fen,Wang, Shao-Hong,Shen, Jin-Hui,Li, En-Min,Xu, Li-Yan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.13
Fascin-1 (FSCN1) is an actin-bundling protein that induces cell membrane protrusions, increases cell motility, and is overexpressed in various human epithelial cancers, including esophageal squamous cell carcinoma (ESCC). We analyzed various protein-protein interactions (PPI) of differentially-expressed genes (DEGs), in fascin knockdown ESCC cells, to explore the role of fascin overexpression. The node-degree distributions indicated these PPI sub-networks to be characterized as scale-free. Subcellular localization analysis revealed DEGs to interact with other proteins directly or indirectly, distributed in multiple layers of extracellular membrane-cytoskeleton/ cytoplasm-nucleus. The functional annotation map revealed hundreds of significant gene ontology (GO) terms, especially those associated with cytoskeleton organization of FSCN1. The Random Walk with Restart algorithm was applied to identify the prioritizations of these DEGs when considering their relationship with FSCN1. These analyses based on PPI network have greatly expanded our comprehension of the mRNA expression profile following fascin knockdown to future examine the roles and mechanisms of fascin action.
Soft Ionization of Metallo-Mefenamic Using Electrospray Ionization Mass Spectrometry
( Hani Nasser Abdelhamid ),( Hui Fen Wu ) 한국질량분석학회 2015 Mass spectrometry letters Vol.6 No.2
Detection of mefenamic acid (M, non-steroidal anti-inflammatory drug, NSAIDs) and its metallodrug was investigated using electrospray ionization mass spectrometry (ESI-MS) and fluorescence spectroscopy. ESI-MS data (500 μL, 1×10-3 M) revealed high detection sensitivity for the drug and metallodrug. ESI-MS spectra revealed peaks at 242, 580, and 777 Da corresponding to [M+H]+, [63Cu(M-H)2(H2O)2+H]+, and [56Fe(M-H)3+H]+, respectively. The metal:mefenamic ratios of ESIMS spectra are in complete agreement with the fluorescence spectroscopy results (1:2 for Cu(II) and 1:3 for Fe(III)). ESI is a soft ionization technique that can be used on labile metallo-mefenamic acids and is promising for the detection of these species in environmental samples and biological fluids.