The Gut Microbiota of Pregnant Rats Alleviates Fetal Growth Restriction by Inhibiting the TLR9/MyD88 Pathway

Tang Hui,Li Hanmei,Li Dan,Peng Jing,Zhang Xian,Yang Weitao 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.9

Fetal growth restriction (FGR) is a prevalent obstetric condition. This study aimed to investigate the role of Toll-like receptor 9 (TLR9) in regulating the inflammatory response and gut microbiota structure in FGR. An FGR animal model was established in rats, and ODN1668 and hydroxychloroquine (HCQ) were administered. Changes in gut microbiota structure were assessed using 16S rRNA sequencing, and fecal microbiota transplantation (FMT) was conducted. HTR-8/Svneo cells were treated with ODN1668 and HCQ to evaluate cell growth. Histopathological analysis was performed, and relative factor levels were measured. The results showed that FGR rats exhibited elevated levels of TLR9 and myeloid differentiating primary response gene 88 (MyD88). In vitro experiments demonstrated that TLR9 inhibited trophoblast cell proliferation and invasion. TLR9 upregulated lipopolysaccharide (LPS), LPS-binding protein (LBP), interleukin (IL)-1β and tumor necrosis factor (TNF)-α while downregulating IL-10. TLR9 activated the TARF3-TBK1-IRF3 signaling pathway. In vivo experiments showed HCQ reduced inflammation in FGR rats, and the relative cytokine expression followed a similar trend to that observed in vitro. TLR9 stimulated neutrophil activation. HCQ in FGR rats resulted in changes in the abundance of Eubacterium_coprostanoligenes_group at the family level and the abundance of Eubacterium_coprostanoligenes_group and Bacteroides at the genus level. TLR9 and associated inflammatory factors were correlated with Bacteroides, Prevotella, Streptococcus, and Prevotellaceae_Ga6A1_group. FMT from FGR rats interfered with the therapeutic effects of HCQ. In conclusion, our findings suggest that TLR9 regulates the inflammatory response and gut microbiota structure in FGR, providing new insights into the pathogenesis of FGR and suggesting potential therapeutic interventions.

Potassium hydroxide activation of activated carbon:a commentary

Tang Shu Hui,Muhammad Abbas Ahmad Zaini 한국탄소학회 2015 Carbon Letters Vol.16 No.4

Synthesis and properties of hydroxyapatite-containing coating on AZ31 magnesium alloy by micro-arc oxidation

Hui Tang 한국표면공학회 2023 한국표면공학회 학술발표회 초록집 Vol.2023 No.11-1

Valorization of spent activated carbon in glycerine deodorization unit for methylene blue removal

Hui Tang Shu,Zaini Muhammad Abbas Ahmad 한국탄소학회 2021 Carbon Letters Vol.31 No.4

Spent Calgon Filtrasorb activated carbon (SAC) from glycerine deodorization unit was evaluated for the removal of methylene blue (MB). The SAC was used without further modifcation. The SAC was characterized for BET surface area, pH, pHpzc and FTIR to determine the textural and chemical properties of SAC. The batch adsorption study of MB was carried out under diferent initial concentrations (5–500 mg/L), pH (2–11) and contact time (0–200 h). The SAC was found to have high BET surface area, pore volume and average pore diameter of 735 m2 /g, 0.292 cm3 /g and 2.56 nm, respectively. The properties of SAC contributed to high MB adsorption capacity of 283 mg/g. The equilibrium data ftted well with Langmuir model, indicating monolayer adsorption; while the activation energy (Ea) of Dubinin–Radushkevitch (D–R) model is lower than 8 kJ/mol, signifying physisorption. The adsorption kinetics was best illustrated by pseudo-second-order model, while the intraparticle difusion and Boyd models suggested that flm difusion is the rate-controlling step. These fndings showed that Calgon Filtrasorb SAC from glycerine deodorization unit can be potentially reused an adsorbent for the removal of dyes.

Influence of Co(CH3COO)2 concentration on thermal emissivity of coatings formed on titanium alloy by micro-arc oxidation

Hui Tang,Qiu Sun,Tiezhu Xin,Chuangui Yi,Zhaohua Jiang,Fuping Wang 한국물리학회 2012 Current Applied Physics Vol.12 No.1

Ceramic thermal protection coatings on Ti6Al4V alloy were achieved by micro-arc oxidation (MAO) in the presence of Co(CH3COO)2. The morphology, crystallographic structure and chemical composition of the coating were characterized by various techniques. The thermal emission of the coating was measured by Fourier transform spectrometer apparatus. The bonding strength between the coating and substrate was studied, together with the thermal shock resistance of the coating. The results indicate that the content of Co in the coating layer significantly affects its thermal emissivity. Higher concentration of Co(CH3-COO)2 in electrolytes leads to more Co ions into the coating, which enhances the emissivity of the coating. All the coatings show bonding strength higher than 10 MPa. In addition, the coating remains stable over 40 cycles of thermal shocking. The coating formed at 4 g/L Co(CH3COO)2 displays an average spectral emissivity value more than 0.9 and bonding strength about 10.4 MPa.

Monosaccharide as a Central Scaffold Toward the Construction of Salicylate-Based Bidentate PTP1B Inhibitors via Click Chemistry

Tang, Yan-Hui,Hu, Min,He, Xiao-Peng,Fahnbulleh, Sando,Li, Cui,Gao, Li-Xin,Sheng, Li,Tang, Yun,Li, Jia,Chen, Guo-Rong Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.3

The discovery of carbohydrate-based bioactive compounds has recently received considerable interest in the drug development. This paper stresses on the application of 1-methoxy-O-glucoside as the central scaffold, whereas salicylic pharmacophores were introduced with diverse spatial orientations probing into the structural preference of an enzymatic target, i.e. protein tyrosine phosphatase 1B (PTP1B). By employing regioselective protection and deprotection strategy, 2,6-, 3,4-, 4,6- and 2,3-di-O-propynyl 1-methoxy-O-glucosides were previously synthesized and then coupled with azido salicylate via click chemistry in forming the desired bidentate salicylic glucosides with high yields. The inhibitory assay of the obtained triazolyl derivatives leads to the identification of the 2,3-disubstituted salicylic 1-methoxy-O-glucoside as the structurally privileged PTP1B inhibitor among this bidentate compound series with micromole-ranged $IC_{50}$ value and reasonable selectivity over other homologous PTPs tested. In addition, docking simulation was conducted to propose a plausible binding mode of this authorized inhibitor with PTP1B. This research might furnish new insight toward the construction of structurally different bioactive compounds based on the monosaccharide scaffold.

An Energy Harvesting Aware Routing Algorithm for Hierarchical Clustering Wireless Sensor Networks

( Chaowei Tang ),( Qian Tan ),( Yanni Han ),( Wei An ),( Haibo Li ),( Hui Tang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.2

Recently, energy harvesting technology has been integrated into wireless sensor networks to ameliorate the nodes` energy limitation problem. In theory, the wireless sensor node equipped with an energy harvesting module can work permanently until hardware failures happen. However, due to the change of power supply, the traditional hierarchical network routing protocol can not be effectively adopted in energy harvesting wireless sensor networks. In this paper, we improve the Low-Energy Adaptive Clustering Hierarchy (LEACH) protocol to make it suitable for the energy harvesting wireless sensor networks. Specifically, the cluster heads are selected according to the estimation of nodes` harvested energy and consumed energy. Preference is given to the nodes with high harvested energy while taking the energy consumption rate into account. The utilization of harvested energy is mathematically formulated as a max-min optimization problem which maximizes the minimum energy conservation of each node. We have proved that maximizing the minimum energy conservation is an NP-hard problem theoretically. Thus, a polynomial time algorithm has been proposed to derive the near-optimal performance. Extensive simulation results show that our proposed routing scheme outperforms previous works in terms of energy conservation and balanced distribution.

Monosaccharide as a Central Scaffold Toward the Construction of Salicylate-Based Bidentate PTP1B Inhibitors via Click Chemistry

Yan-Hui Tang,Min Hu,Xiao-Peng He,Sando Fahnbulleh,Cui Li,Li-Xin Gao,Li Sheng,Yun Tang,Jia Li,Guo-Rong Chen 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.3

The discovery of carbohydrate-based bioactive compounds has recently received considerable interest in the drug development. This paper stresses on the application of 1-methoxy-O-glucoside as the central scaffold,whereas salicylic pharmacophores were introduced with diverse spatial orientations probing into the structural preference of an enzymatic target, i.e. protein tyrosine phosphatase 1B (PTP1B). By employing regioselective protection and deprotection strategy, 2,6-, 3,4-, 4,6- and 2,3-di-O-propynyl 1-methoxy-O-glucosides were previously synthesized and then coupled with azido salicylate via click chemistry in forming the desired bidentate salicylic glucosides with high yields. The inhibitory assay of the obtained triazolyl derivatives leads to the identification of the 2,3-disubstituted salicylic 1-methoxy-O-glucoside as the structurally privileged PTP1B inhibitor among this bidentate compound series with micromole-ranged IC50 value and reasonable selectivity over other homologous PTPs tested. In addition, docking simulation was conducted to propose a plausible binding mode of this authorized inhibitor with PTP1B. This research might furnish new insight toward the construction of structurally different bioactive compounds based on the monosaccharide scaffold.

Microsatellite Markers Linked to Quantitative Trait Loci Affecting Fatness in Divergently Selected Chicken Lines for Abdominal Fat

Zhang, Hui,Wang, Shouzhi,Li, Hui,Yu, Xijiang,Li, Ning,Zhang, Qin,Liu, Xiaofeng,Wang, Qigui,Hu, Xiaoxiang,Wang, Yuxiang,Tang, Zhiquan Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.10

Abdominal fat characters are complex and economically important in the poultry industry. Their selection may benefit from the implementation of marker-assisted selection (MAS). The objective of this study was to identify the markers linked to QTL responsible for fatness traits. The Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) were used in the study. A total of 596 individuals from the divergent tails from the 6th to the 10th generations were genotyped at 23 microsatellite markers on chromosome 1. The differences of allele frequencies of all marker alleles between the divergent tails across the five generations were recorded. The allele frequencies of five markers, including LEI0209, LEI0146, MCW0036, ADL328 and MCW0115, had significant differences between the two tails in all five generations. The resulting p-values using Fisher's exact test on eleven markers, containing MCW248, MCW0010, MCW0106, LEI0252, LEI0068, MCW0018, MCW0061, LEI0088, MCW200, MCW283 and ROS0025, had a decreasing tendency from the 6th to the 10th generation. Statistical analysis showed that polymorphisms of the eight markers, including LEI0209, LEI0146, ROS0025, MCW0115, MCW0010, MCW0036, MCW283, ADL328, were significantly (p<0.0011) or suggestively (p<0.05) associated with abdominal fat content (AFW and AFP) across generations. It is concluded that the eight markers could be associated with the QTL affecting the deposition of abdominal fat in broiler chickens.

Research on Preparation of Ni/SiO2 Optical Attenuation Slice by Magnetron Sputtering

Lei Li,Hui Tang,Shanshan Hao,Wenxue Wang 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.12

In order to find new methods for preparing and improving the performance of optical attenuation slice. Vacuum magnetron sputtering method was used to prepare Ni/SiO2 composite film optical attenuation slices, with vacuum magnetron sputtering apparatus, at 5,10,15,20,25min sputtering time, 0.2,0.4,0.6,0.8,1.0Pa sputtering pressure and sputtering power 300~1200W. The XRD, SEM, EDS, AFM and 722 spectrophotometer also were used to study the effects of different sputtering time, sputtering pressure and sputtering power on the film structure, surface morphology, composition, three-dimensional structure, surface roughness and light attenuation rate of optical attenuation slice samples. The results indicated that: Ni/SiO2 composite films were formed, instead of simple physical adsorption between Ni film and SiO2 substrates with magnetron sputtering by XRD analysis and calculation, with uniform grain size of 25.96, 32.38, 32.29,26.95, 25.92nm, respectively. The main component element was Ni, but there were few impurities deposited on the substrates. Impurities were reduced gradually with the increase of sputtering time, mainly resulted from two sputtering of Ni atoms by EDS; The film surface was smooth and dense, flatness and organizational structure of Ni film were better, surface roughness was 1.267nm at 25min sputtering time, 0.4Pa pressure and 400W power with SEM and AFM. Light attenuation rate of optical attenuation slices was different in different process parameters, and the maximum reached 0.52, film flatness, impurities and defects were the main reason by the light attenuation performance analysis.