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( Hui-ying Low ),( Hsiu-hui Chen ),( Chun-chia Huang ),( Tsung-hsien Lee ),( Chung-i Chen ),( Lii-sheng Huang ),( Maw-sheng Lee ) 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
Study Question: To evaluate the most ideal interval between blastocyst biopsy and vitrification in preimplantation genetic screening (PGS). Study Design, Size, Duration: This is a retrospective study and total 224 patients underwent the PGS from 2012 Dec. to 2015 Mar. All of patients underwent blastocyst vitrification after biopsy and 1~2 euploid blastocyst for transfer after warming. The primary outcome measures were the implantation and pregnancy rates per PGS-frozen embryo transfer cycle. Materials, Setting, Methods: The blastocyst grading including grade 4, 5 and 6 (according to Gardner grading system) on day 5 or day 6 were selected for trophectoderm biopsy. All blastocyst underwent vitrification immediately (interval: 0.5 hour) or 1 to 7 hours after biopsy. At the time of vitrification the grade of blastocyst expansion was also recorded. All patients were divided into two groups according to the grade of expanded (Group1: ≤1/2 expansion (n=41), Group2: ≥3/4 expansion (n=183)). Furthermore, combined two factors including the interval and morphology of blastocyst after biopsy, all patients were further divided into interval 1 (<3 hours and ≤1/2 expansion) and interval 2 (≥3 hours and ≥3/4 expansion). The morphologically best euploid blastocyst(s) (1~2 embryos) was/were selected first for transfer on the next cycle. Main Results: Assessment morphology of blastocyst after biopsy in different interval, at 0.5 hour after biopsy, 100% blastocyst was non-expansion; at 1 hour after biopsy, only 17% blastocyst was 3/4 expansion or all-expansion; at 3 hours after biopsy, 86% blastocyst was 3/4 expansion or all-expansion and after 5.5 hours, 100% blastocyst was all-expansion or hatching. All blastocysts were survival (100%, 359/359) after warming. The mean of embryo transfer number between all groups were no significantly difference. The implantation rate in Group2 (63.4%) was significantly higher than that in Group1 (46.9%, p=0.014). The pregnancy rates in Group4 (73.8%) was sig-nificantly higher than that in Group1 (51.2%, p=0.004). The implantation and pregnancy rates in the group of embryo ≥3/4 expansion combined with ≥3 hours after biopsy (63.6%, 178/280; 73.8%, 127/172) were significantly higher than that in the group of ≤1/2 expansion with <3 hour (45.6, 26/57; 50.0%, 18/36; p=0.0113 and p=0.0056, respectively). Conclusion: The most ideal interval between biopsy and vitrification was least 3 hours and ≥3/4 expansion of blastocyst after biopsy could improve the implantation and pregnancy rates for PGS.
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Tsai Yu-Chen,Cheng Tai-Shan,Liao Hsiu-Jung,Chuang Ming-Hsi,Chen Hui-Ting,Chen Chun-Hung,Zhang Kai-Ling,Chang Chih-Hung,Lin Po-Cheng,Huang Chi-Ying F. 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6
BACKGROUND: Extracellular vesicles (EVs) are derived from internal cellular compartments, and have potential as a diagnostic and therapeutic tool in degenerative disease associated with aging. Mesenchymal stem cells (MSCs) have become a promising tool for functional EVs production. This study investigated the efficacy of EVs and its effect on differentiation capacity. METHODS: The characteristics of MSCs were evaluated by flow cytometry and stem cell differentiation analysis, and a production mode of functional EVs was scaled from MSCs. The concentration and size of EVs were quantitated by Nanoparticle Tracking Analysis (NTA). Western blot analysis was used to assess the protein expression of exosomespecific markers. The effects of MSC-derived EVs were assessed by chondrogenic and adipogenic differentiation analyses and histological observation. RESULTS: The range of the particle size of adipose-derived stem cells (ADSCs)- and Wharton’s jelly -MSCs-derived EVs were from 130 to 150 nm as measured by NTA, which showed positive expression of exosomal markers. The chondrogenic induction ability was weakened in the absence of EVs in vitro. Interestingly, after EV administration, type II collagen, a major component in the cartilage extracellular matrix, was upregulated compared to the EV-free condition. Moreover, EVs decreased the lipid accumulation rate during adipogenic induction. CONCLUSION: The results indicated that the production model could facilitate production of effective EVs and further demonstrated the role of MSC-derived EVs in cell differentiation. MSC-derived EVs could be successfully used in cell-free therapy to guide chondrogenic differentiation of ADSC for future clinical applications in cartilage regeneration.
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DESTINATION BRAND COOLNESS: CONCEPTUALIZATION AND SCALE DEVELOPMENT
Hsiu-Yu Teng,Cheng-Hsien Tsai,Chia-Wen Hung,Hui-Chen Hung 글로벌지식마케팅경영학회 2023 Global Marketing Conference Vol.2023 No.07
Destination branding has become an important trend in modern tourism. The development of destination brands has become a strategic tool worldwide because of the growing competition between destinations. In recent years, many tourist destinations have combined unique, creative, and attractive elements to transform themselves into “cool” tourist destinations. Destination brand coolness refers to tourists’ subjective and positive perception of tourist destinations and their beliefs that the destination brand offer distinctive, novel characteristics and attributes that the visitors are attracted to. Creating destination brand coolness can help tourist destinations differentiate themselves from their competitive counterparts, thereby attracting tourists. However, no study has conceptualized the construct of destination brand coolness, let alone developing scales that measure destination brand coolness.
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The roles of a university professor in a teacher study group
Hui-Chin Yeh,Yi-Ping Chen,Hsiu-Ting Hung 서울대학교 교육연구소 2012 Asia Pacific Education Review Vol.13 No.3
The opportunities in which university professors collaborate with the practicing school teachers in a teacher study group are few. This study investigated how a university professor facilitated a collaborative teacher study group to enhance teachers' professional growth. Five primary school teachers and a university professor collaborated on incorporating Reader's Theatre into the design and revision of the curriculum in a teacher study group. This study not only identified the roles of the university professor but also highlighted how this outside stimulus facilitated the process of teachers' professional dialogues in this teacher study group. Five roles of the professor in the teacher study group were identified, including content expert, information provider, thought challenger, discussion facilitator, and caring listener. The discrepancies toward the roles that the professor in the teacher study group should serve between the elementary school teachers and the university professor were analyzed and reported. Implications were drawn for both classroom teachers and university professors.
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Su, Chin-Hui,Cheng, Chih-Hsiang,Lin, Jung-Hsin,Liu, Hsiu-Chou,Yu, Yen-Ting,Lin, Chai-Ching,Chen, Wei-Jung Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.11
Objective: The objective of this experiment was to investigate the effects of different colors produced by light emitting diode (LED) on Brown Tsaiya ducks. Methods: A total of 144 female Brown Tsaiya ducks were randomly allocated into three individual cage rearing chambers with different LED illumination colors as treatments. Three different treatments were: i) white color, ii) blue color, and iii) red color. The experiment periods were from ducks 21 to 49 weeks of age, determined traits included i) egg laying performance, ii) feed intake, iii) egg shell breaking strength, iv) egg shell thickness, v) egg Haugh unit, vi) egg weight, vii) serum Estradiol and Progesterone concentration, and viii) behavior pattern. Results: The results indicated that when compared with white and blue color, red color could stimulate ducks sexual maturation and raised the egg laying performance. The red light group was also observed to have the highest feed intake among three treatments. The blue treatment had the lowest egg shell breaking strength and the highest egg weight among three treatments, nevertheless, no significant difference was observed among three treatments on egg shell thickness and egg Haugh unit. The red light group had higher serum estradiol concentration than the white and blue groups, but no significant difference among treatments on the serum Progesterone concentration was found. The results of behavior pattern indicated that red light group showed more feeding and less resting behavior compared to the blue light group. Conclusion: We found a potential of applying red light illumination in the indoor laying duck raising system with positive results on egg laying performance and acceptable egg weight, equivalent egg qualities compared to white and blue light.
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Hsu-Feng Lu,Yu-Heng Lai,Hsiu-Chen Huang,I-Jung Lee,Lie-Chwen Lin,Hui-Kang Liu,Hsiao-Hsuan Tien,Cheng Huang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.2
Background: Dietary fat has been suggested to be the cause of various health issues. Obesity, hypertension,cardiovascular disease, diabetes, dyslipidemia, and kidney disease are known to be associatedwith a high-fat diet (HFD). Obesity and associated conditions, such as type 2 diabetes mellitus andnonalcoholic fatty liver disease (NAFLD), are currently a worldwide health problem. Few prospectivepharmaceutical therapies that directly target NAFLD are available at present. A Traditional ChineseMedicine, ginseng-plus-Bai-Hu-Tang (GBHT), is widely used by diabetic patients to control glucose levelor thirst. However, whether it has therapeutic effects on fat-induced hepatic steatosis and metabolicsyndrome remains unclear. Methods: This study was conducted to examine the therapeutic effect of GBHT on fat-induced obesity,hepatic steatosis, and insulin resistance in mice. Results: GBHT protected mice against HFD-induced body weight gain, hyperlipidemia, andhyperglycemia compared with mice that were not treated. GBHT inhibited the expansion of adiposetissue and adipocyte hypertrophy. No ectopic fat deposition was found in the livers of HFD mice treatedwith GBHT. In addition, glucose intolerance and insulin sensitivity in HFD mice was also improved byGBHT. Conclusion: GBHT prevents changes in lipid and carbohydrate metabolism in a HFD mouse model. Ourfindings provide evidence for the traditional use of GBHT as therapy for the management of metabolicsyndrome.
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Lu, Hsu-Feng,Lai, Yu-Heng,Huang, Hsiu-Chen,Lee, I-Jung,Lin, Lie-Chwen,Liu, Hui-Kang,Tien, Hsiao-Hsuan,Huang, Cheng The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.2
Background: Dietary fat has been suggested to be the cause of various health issues. Obesity, hypertension, cardiovascular disease, diabetes, dyslipidemia, and kidney disease are known to be associated with a high-fat diet (HFD). Obesity and associated conditions, such as type 2 diabetes mellitus and nonalcoholic fatty liver disease (NAFLD), are currently a worldwide health problem. Few prospective pharmaceutical therapies that directly target NAFLD are available at present. A Traditional Chinese Medicine, ginseng-plus-Bai-Hu-Tang (GBHT), is widely used by diabetic patients to control glucose level or thirst. However, whether it has therapeutic effects on fat-induced hepatic steatosis and metabolic syndrome remains unclear. Methods: This study was conducted to examine the therapeutic effect of GBHT on fat-induced obesity, hepatic steatosis, and insulin resistance in mice. Results: GBHT protected mice against HFD-induced body weight gain, hyperlipidemia, and hyperglycemia compared with mice that were not treated. GBHT inhibited the expansion of adipose tissue and adipocyte hypertrophy. No ectopic fat deposition was found in the livers of HFD mice treated with GBHT. In addition, glucose intolerance and insulin sensitivity in HFD mice was also improved by GBHT. Conclusion: GBHT prevents changes in lipid and carbohydrate metabolism in a HFD mouse model. Our findings provide evidence for the traditional use of GBHT as therapy for the management of metabolic syndrome.
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