Carbon monoxide prevents TNF-α-induced eNOS downregulation by inhibiting NF-κB-responsive miR-155-5p biogenesis

Choi, Seunghwan,Kim, Joohwan,Kim, Ji-Hee,Lee, Dong-Keon,Park, Wonjin,Park, Minsik,Kim, Suji,Hwang, Jong Yun,Won, Moo-Ho,Choi, Yoon Kyung,Ryoo, Sungwoo,Ha, Kwon-Soo,Kwon, Young-Guen,Kim, Young-Myeong Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.11

<P>Heme oxygenase-1-derived carbon monoxide prevents inflammatory vascular disorders. To date, there is no clear evidence that HO-1/CO prevents endothelial dysfunction associated with the downregulation of endothelial NO synthesis in human endothelial cells stimulated with TNF-α. Here, we found that the CO-releasing compound CORM-2 prevented TNF-α-mediated decreases in eNOS expression and NO/cGMP production, without affecting eNOS promoter activity, by maintaining the functional activity of the <I>eNOS</I> mRNA 3′-untranslated region. By contrast, CORM-2 inhibited MIR155HG expression and miR-155-5p biogenesis in TNF-α-stimulated endothelial cells, resulting in recovery of the 3′-UTR activity of <I>eNOS</I> mRNA, a target of miR-155-5p. The beneficial effect of CORM-2 was blocked by an NF-κB inhibitor, a miR-155-5p mimic, a HO-1 inhibitor and siRNA against HO-1, indicating that CO rescues TNF-α-induced eNOS downregulation through NF-κB-responsive miR-155-5p expression via HO-1 induction; similar protective effects of ectopic HO-1 expression and bilirubin were observed in endothelial cells treated with TNF-α. Moreover, heme degradation products, except iron and <I>N</I>-acetylcysteine prevented H<SUB>2</SUB>O<SUB>2</SUB>-mediated miR-155-5p biogenesis and eNOS downregulation. These data demonstrate that CO prevents TNF-α-mediated eNOS downregulation by inhibiting redox-sensitive miR-155-5p biogenesis through a positive forward circuit between CO and HO-1 induction. This circuit may play an important preventive role in inflammatory endothelial dysfunction associated with human vascular diseases.</P>

FK506이 T 림프구 사멸에서 활성산소 생성에 미치는 영향

이호균(Ho Kyun Lee),정상영(Sang Young Chung),최수진나(Soo Jin Na Choi) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.5

Purpose: Tacrolimus (FK506) has been widely used as an immunosuppressant in organ transplanted recipients to suppress organ rejection phenomenon. We investigated the role of oxidative stress and heme oxygense-1 by FK506 on human Jurkat T cells. Methods: The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry. Results: Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H₂O₂ and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1. Conclusion: Increase of HO-1 expression would induce to decrease the intracellular H₂O₂ and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.

수주 변영로의 초기 문학에 나타난 방랑과 이상적 공동체론

최호영 ( Choi Ho-young ) 서울대학교 인문학연구원 2016 人文論叢 Vol.73 No.3

이 글의 목적은 수주 변영로의 초기 문학에 나타나는 방랑의 모티프를 구체적으로 고찰하고 그에 따라 탐색하고자 하는 이상적 공동체의 상(像)과 의의를 해명하는데 있다. 변영로의 초기 시들을 검토해보면 일관적으로 상실한 ‘님’에 대한 지향성을 ‘방랑’과 ‘꿈’의 반복성을 통해 표현하고 있다. 이러한 시도는 ‘님’=‘조선’이 그에게 ‘생명’의 근원이라는 것을 보여주기 위함이었다. 여기서 나아가 그는 ‘조선’을 ‘생명’의 근원인 ‘정신’에 위치시키고 있을 뿐만 아니라 이를 탐색하기 위해 상징주의나 신비주의를 수용하기도 하였다. 이를 토대로 그는 ‘개성’을 도입하여 개인의 주체성과 조선적 특이성이 세계적 보편성으로 확장될수 있는 공동체를 구상하고자 했다. 1920년대 중반으로 접어들면서 변영로는 방랑을 기행의 방식으로 구체화하고 있으며 당대 문단에서 대표적인 전통적 양식으로 거론되었던 시조를 차용하여 이상적 공동체를 모색하려는 시도를 보여준다. 그가 특히 민족사의 기원이라 할 수 있는 백두산을 기행하고 이를 시조에 담아내려 한 것은 단순히 민족정신을 고취하기 위한 것이라기보다 그가 구상해오던 이상적 공동체의 원형을 탐색하기 위한 것이었다. 이는 그와 영향관계에 놓인 단재 신채호와 위당 정인보의 역사서에서도 확인할 수 있다. 따라서 이 글은 변영로의 초기 문학을 민족주의의 이념에 환원하려는 관행에서 벗어나 거기에 얽혀있는 풍부한 의미망들을 읽어냄으로써 1920년대 초기 문학의 ‘공동체’에 대한 논의가 더욱 생산적인 방향으로 전개되기를 기대한다. The purpose of this paper is to specifically examine the mode of wandering consistently represented in Suju Byeon, Young-ro’s early poems and to elucidate the significance and form of ideal community. Byeon, Young-ro’s early poems revealed the intentionality of the subject called ‘Nim’ by expressing repeatability of ‘dream’ and ‘wandering’, thereby showing that it was the origin of ‘life’ to him. Based on this, he came up with the idea of community where Joseons particularity and individual independence could be expanded into a global universality through the introduction of ‘individuality’. In the mid-1920s Byeon, Young-ro specified wandering into the method of travel and explored ideal community by borrowing sijo(traditional three-verse Korean poem) discussed as a classical form in the contemporary literary world. This point can be verified with the historical book written by Danjae Shin, Chae-ho and Widang Jeong, In-bo. Therefore, this paper considers the mode of wandering in his early poems as the device to investigate community, which can be an alternative of the reality of Joseon period, and to explore the origin of ‘life’ rather than as returning to the ideology of nationalism.

가속도계를 이용한 재료의 영계수 측정방법

손창호(Sohn, Chang-Ho),박진호(Park, Jin-Ho),윤두병(Yoon, Doo-Byung),정의필(Chong, Ui-Pil),최영철(Choi, Young-Chul) 한국소음진동공학회 2006 한국소음진동공학회 논문집 Vol.16 No.11

For the description of the elastic properties of linear objects a convenient parameter is the ratio of the stress to the strain, a parameter called the Young's modulus of the material. Young's modulus can be used to predict the elongation or compression of an object as long as the stress is less than the yield strength of the material. Conventional method for estimating Young's modulus measured the ratio of stress to corresponding strain below the proportional limit of a material using a tensile testing machine. But the method needs precision specimens and expensive equipment. In this paper, we proposed method for estimating Young's modulus using accelerometer. The basic idea comes from that the wave velocity is different as the Young's modulus. To obtain Young's modulus, a group velocity is obtained. It is difficult to measure group velocity. This is because plate medium has a dispersive characteristics which has different wave speed as frequency. In this paper, we used Wigner-Ville distribution to measure group velocity. To verify the proposed method, steel and acryl plate experiments have been performed. Experimental results show that the proposed method is powerful for estimating Young's modulus.

운동강도의 차이가 대장에서의 Heme oxygenase-1 발현에 미치는 영향: Oligonucleotide chip microarray analysis

최은주 ( Eun Ju Choi ),류호영 ( Ho Young Ryu ),차광석 ( Kwang Suk Cha ) 한국운동생리학회(구-한국운동과학회) 2012 운동과학 Vol.21 No.1

이 연구의 목적은 운동강도 차이에 의한 대장조직이 Heme oxygenase-1(HO-1)의 mRNA 발현에 어떠한 영향을 미치는지 규명하기 위해, Sprage-Dawley계 흰쥐를 대상으로 통제그룹(CON)과 저강도 운동그룹(LIE), 고강도 운동그룹(HIE)으로 구분하여 4주 동안 트레드밀운동 후 48시간 이후에 High through-put microarray analysis방법으로 다음과 같은 실험을 실시하였다. 본 실험은 Rat ABI oligo chip 26,857 개의 유전자 중 filtering을 통하여 12.079개 유전지를 선택하였고, 이 중 유의성 있는(p<.05) 유전자 12,072개를 선별하였으며, clustering분석 방법과 면역조직화학염색법에서도 HO-l이 운동강도에 따라 유의하게 발현하였다(p<.05). Microarray분석 후 RT-PCR로 확인한 결과 HO-I의 유전자 발현양상이 일치하는 결과를 얻었다. 이 실험의 결과로서 운동이 대장에서 HO-1 발현에 영향을 주는 것을 알 수 있었다. 운동이 대장에서의 mRNA 발현이 저강도 운동그룹(LIE)보다 고강도 운동그룹(HIE)에서 더 많이 발현되어지는 것으로 보아 고강도 운동에 의해 발생되는 산소라디칼을 HO-l의 유도를 통하여 적절히 제거함으로써 스스로 항상성을 유지하고 있는 것으로 생각되어진다. The purpose of this study is to identify how large intestine tissue effects to mRNA expression in Heme oxygenase-l (HO-1) due to differences in exercise intensity. To perform this experiment we divided Sprage-Dawley rats into 3 groups (control group (CON). low-intensity exercise group (LIE) and high-intensity exercise group (HIE)), and in 48 hours after 4 weeks treadmill exercise. the following experiment was performed with "High through-put micro array analysis methods" . 12,079 genes were chosen by filtering of the Rat ASI oligo chip 26.857 genes in this experiment Among 12.079 genes, we selected significant genes (p<.05) that were also expressed in the ways of clustering analysis and immunohistochemistry. With results of RT-PCR confirming and microarray analyzing, we derived that gene expression profiles of HO-l had been consistent. As a result of this experiment, we certainly identified that exercise effects to HQ-l expression in large intestine. mRNA expression was more expressed in high-intensity exercise group CHIE) than low-intensity exercise group (LIE), it seems that homeostasis maintains itself by properly removing oxygen radical. caused by high intensity exercise. through reduction of HO-l.

Comparison of trophic factors changes in the hippocampal CA1 region between the young and adult gerbil induced by transient cerebral ischemia.

Yan, Bing Chun,Park, Joon Ha,Kim, Sung Koo,Choi, Jung Hoon,Lee, Choong Hyun,Yoo, Ki-Yeon,Kwon, Young-Geun,Kim, Young-Myeong,Kim, Jong-Dai,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2012 Cellular and molecular neurobiology Vol.32 No.8

<P>In the present study, we investigated neuronal death/damage in the gerbil hippocampal CA1 region (CA1) and compared changes in some trophic factors, such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF), in the CA1 between the adult and young gerbils after 5 min of transient cerebral ischemia. Most of pyramidal neurons (89%) were damaged 4 days after ischemia-reperfusion (I-R) in the adult; however, in the young, about 59% of pyramidal neurons were damaged 7 days after I-R. The immunoreactivity and levels of BDNF and VEGF, not GDNF, in the CA1 of the normal young were lower than those in the normal adult. Four days after I-R in the adult group, the immunoreactivity and levels of BDNF and VEGF were distinctively decreased, and the immunoreactivity and level of GDNF were increased. However, in the young group, all of their immunoreactivities and levels were much higher than those in the normal young group. From 7 days after I-R, all the immunoreactivities and levels were apparently decreased compared to those of the normal adult and young. In brief, we confirmed our recent finding: more delayed and less neuronal death occurred in the young following I-R, and we newly found that the immunoreactivities of trophic factors, such as BDNF, GDNF, and VEGF, in the stratum pyramidale of the CA1 in the young gerbil were much higher than those in the adult gerbil 4 days after transient cerebral ischemia.</P>

AN IN-SITU YOUNG'S MODULUS MEASUREMENT TECHNIQUE FOR NUCLEAR POWER PLANTS USING TIME-FREQUENCY ANALYSIS

Choi, Young-Chul,Yoon, Doo-Byung,Park, Jin-Ho,Kwon, Hyun-Sang Korean Nuclear Society 2009 Nuclear Engineering and Technology Vol.41 No.3

Elastic wave is one of the most useful tools for non-destructive tests in nuclear power plants. Since the elastic properties are indispensable for analyzing the behaviors of elastic waves, they should be predetermined within an acceptable accuracy. Nuclear power plants are exposed to harsh environmental conditions and hence the structures are degraded. It means that the Young's modulus becomes unreliable and in-situ measurement of Young's modulus is required from an engineering point of view. Young's modulus is estimated from the group velocity of propagating waves. Because the flexural wave of a plate is inherently dispersive, the group velocity is not clearly evaluated in temporal signal analysis. In order to overcome such ambiguity in estimation of group velocity, Wigner-Ville distribution as the time-frequency analysis technique was proposed and utilized. To verify the proposed method, experiments for steel and acryl plates were performed with accelerometers. The results show good estimation of the Young's modulus of two plates.

AN IN-SITU YOUNG’S MODULUS MEASUREMENT TECHNIQUE FOR NUCLEAR POWER PLANTS USING TIME-FREQUENCY ANALYSIS

YOUNG-CHUL CHOI,DOO-BYUNG YOON,JIN-HO PARK,권휴상 한국원자력학회 2009 Nuclear Engineering and Technology Vol.41 No.3

Elastic wave is one of the most useful tools for non-destructive tests in nuclear power plants. Since the elastic properties are indispensable for analyzing the behaviors of elastic waves, they should be predetermined within an acceptable accuracy. Nuclear power plants are exposed to harsh environmental conditions and hence the structures are degraded. It means that the Young’s modulus becomes unreliable and in-situ measurement of Young’s modulus is required from an engineering point of view. Young’s modulus is estimated from the group velocity of propagating waves. Because the flexural wave of a plate is inherently dispersive, the group velocity is not clearly evaluated in temporal signal analysis. In order to overcome such ambiguity in estimation of group velocity, Wigner-Ville distribution as the time-frequency analysis technique was proposed and utilized. To verify the proposed method, experiments for steel and acryl plates were performed with accelerometers. The results show good estimation of the Young’s modulus of two plates.

김수영의 ‘언어’ 인식에 관한 존재론적 고찰

최호영(Choi, Ho-Young) 한국어문학회 2018 語文學 Vol.0 No.141

This study explores the ontological aspects contained in Kim Su-young’s view on language, referring to the middle and latter part of Heidegger’s theory of art. Kim had a keen sense of controversy about contemporary Korean society from beginning to late, and he also tried to approach it with respect to the problems of the art in view of the problems of reality. Heidegger’s theory of art would have been part of his reference in finding out the essence of poetry. Therefore, this article focused on ‘Language’ and identified the ontological aspect of Kim’s literature as follows. First, Kim showed his sympathy for Heidegger’s ‘the Language of Existence’ from a relatively early stage. According to his perspectives, a being who runs the everyday world has forgotten the essence of existence in that it turns ‘language’ into a means of communication or an instrument of expression. In this situation, Kim used the language of existence, which reveals the nature of his existence, as an indicator of his poetic language. Next, Kim tried to pursue a ‘powerful’ peace that can overcome the existing reality while overcoming extremes of content and form, artistic value and reality. Through the middle part of Heidegger’s theory of art, he was able to explore the possibility of language changing the reality of those days while approaching the essence of the work. In addition, Kim tried to give up his desire to open the existing world through the latter part of Heidegger’s theory of art and leave it there. For him, the moment that existence itself came with its own voice was like ‘salvation’. Therefore, Kim’s ontological exploration can be seen as an attempt to fundamentally look at the problems of contemporary Korean society and to transform the contradictions of the reality.

Factors associated with the risk of colorectal neoplasia in young adults under age 40

Ilsoo Kim,Han Hee Lee,Young Jae Ko,Ho Eun Chang,Dae Young Cheung,Bo-In Lee,Young-Seok Cho,Jin Il Kim,Myung-Gyu Choi 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.5

Background/Aims: Recent epidemiologic studies have shown a continued increase in colorectal cancer incidence among younger adults. Little is known about the factors that contribute to the development of young-onset colorectal neoplasia (CRN). Methods: A cross-sectional analysis was performed for individuals younger than 40 years who underwent colonoscopy in Seoul St. Mary’s Hospital and its affiliated health screening center. High-risk CRN was defined as adenoma or sessile serrated lesion ≥ 10 mm, with three or more adenomas, villous histology, high grade dysplasia, or carcinoma. Results: Of these 13,621 included participants, 2,023 (14.9%) had one and more CRN. Young patients with CRN tended to be elderly, male, obese, smoker, having a habit of drinking, and having comorbidities such as hypertension, dyslipidemia, diabetes, and chronic kidney disease. In a multivariate analysis adjusted for age, sex, obesity, smoking status, and alcohol intake, old age (odds ratio [OR], 1.086; 95% confidence interval [CI], 1.054 to 1.119), male sex (OR, 1.748; 95% CI, 1.247 to 2.451), obesity (OR, 1.439; 95% CI, 1.133 to 1.828), and smoking (OR, 1.654; 95% CI, 1.287 to 2.127) were independent risk factors for overall CRN. Obesity and smoking as two modifiable factors increased the risk for high-risk CRN even more than for overall CRN (OR, 1.734; 95% CI, 1.168 to 2.575 and OR, 1.797; 95% CI, 1.172 to 2.753, respectively). Conclusions: Obesity and smoking were modifiable risk factors for CRN in young adults. They increased the risk for highrisk CRN even more than for overall CRN. A colonoscopy might be beneficial for young individuals with these factors.