http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Hiyoung Kim ),( Kwang Jin Kim ),( Jeong Tae Yeon ),( Seong Hwan Kim ),( Dong Hwan Won ),( Hyukjae Choi ),( Sang Jip Nam ),( Young Jin Son ),( Heonjoong Kang ) 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0
A new inhibitor, placotylene A (1), of the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation, and a regioisomer of placotylene A, placotylene B (2), were isolated from a Korean marine sponge Placospongia sp. The chemical structures of placotylenes A and B were elucidated on the basis of 1D and 2D
Kim, Hiyoung,Chin, Jungwook,Choi, Hyukjae,Baek, Kyungryul,Lee, Tae-Gu,Park, Seong Eon,Wang, Weihong,Hahn, Dongyup,Yang, Inho,Lee, Jihye,Mun, Bora,Ekins, Merrick,Nam, Sang-Jip,Kang, Heonjoong American Chemical Society 2013 Organic letters Vol.15 No.1
<P>Two unprecedented phosphorus-containing iodinated polyacetylenes, phosphoiodyns A and B (<B>1</B>–<B>2</B>), were isolated from a Korean marine sponge <I>Placospongia</I> sp. Their structures were elucidated by spectroscopic data analysis. Phosphoiodyn A exhibited potent agonistic activity on human peroxisome proliferator-activated receptor delta (hPPARδ) with an EC<SUB>50</SUB> of 23.7 nM.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orlef7/2013/orlef7.2013.15.issue-1/ol3031318/production/images/medium/ol-2012-031318_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ol3031318'>ACS Electronic Supporting Info</A></P>
Kim, Hunseong,Cao, Thao Quyen,Yeo, Chae-eun,Shin, Seung Ho,Kim, Hiyoung,Hong, Dong-Hyuck,Hahn, Dongyup The Korean Society for Microbiology and Biotechnol 2022 Journal of microbiology and biotechnology Vol.32 No.8
Phenanthrenes are bioactive phenolic compounds found in genus Dioscorea, in which they are distributed more in peel than in flesh. Recent studies on phenanthrenes from Dioscorea sp. peels have revealed the potential for valuable biomaterials. Herein, an analytical method using high-performance liquid chromatography (HPLC) for quantitation of bioactive phenanthrenes was developed and validated. The calibration curves were obtained using the phenanthrenes (1-3) previously isolated from Dioscorea batatas concentrations in the range of 0.625-20.00 ㎍/ml with a satisfactory coefficient of determination (R<sup>2</sup>) of 0.999. The limit of detection (LOD) and the limit of quantification (LOQ) values of the isolated phenanthrenes ranged from 0.78-0.89 and 2.38-2.71 ㎍/ml, respectively. The intraday and interday precision ranged from 0.25-7.58%. The recoveries of the isolated phenanthrenes were from 95 to 100% at concentrations of 1.25, 2.5, and 5.0 ㎍/ml. Additionally, phenanthrenes (1-3) were found in all investigated peel extracts. Hence, the developed method was encouraging for the quantitative analysis of phenanthrenes in genus Dioscorea.
Hiyoung KIM,Uijin KIM,Chang-Hun JI,Ha Youn SHIN,Hahk-Soo KANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Streptomyces rapamycinicus is one of the producers of the clinically important immunosupressant rapamycin, and thus has been regarded as a biologically valuable microorganism. This Streptomyces strain possesses an exceptional biosynthetic diversity as the bioinformatic analysis of its genome sequences revealed the presence of total 51 different biosynthetic gene clusters (BGCs) such as PKS and NRPS gene clusters. Among the predicted BGCs in S. rapamycinicus, we have focused on the BGC 41 that is cryptic BGC harboring reducing type II PKS (polyketide synthase) genes predicted to be involved in the polyene biosynthesis which is rare family of natural products. Through phylogenetic analysis, we identified that KS (ketosynthase) genes derived from polyene BGCs form a separate clade from those of aromatic polyketide BGCs in a KS gene phylogeny, suggesting their distinct evolutionary relationships. The new hybrid genotype including reducing T2PKS with T1PKS and fatty acid synthase in BGC 41 afforded structurally new compounds named rapacrycins. To identify its metabolites through heterologous expression, we cloned the BGC 41 using TAR cloning in yeast and inserted into the genome of the heterologous host S. albus. However, no production was observed, and thus promoter engineering was used to activate this silent cluster leading to the production of a series of cluster-specific metabolites, rapacrycins.
( Hiyoung Kim ),( Jungwook Chin ),( Hyukjae Choi ),( Kyungryul Baek ),( Tae Gu Lee ),( Seong Eon Park ),( Weihong Wang ),( Dongyup Hahn ),( Inho Yang ),( Jihye Lee ),( Bora Mun ),( Merrick Ekins ),( S 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
Two unprecedented phosphorus-containing iodinated polyacetylenes, phosphoiodyns A and B (1-2), were isolated from a Korean marine sponge Placospongia sp. Their structures were elucidated by spectroscopic data analysis. Phosphoiodyn A exhibited potent agonistic activity on human peroxisome proliferator-activated receptor delta (hPPARδ) with an EC(50) of 23.7 nM.
Acredinone C and the Effect of Acredinones on Osteoclastogenic and Osteoblastogenic Activity
Yeon, Jeong-Tae,Kim, Hiyoung,Kim, Kwang-Jin,Lee, Jihye,Won, Dong Hwan,Nam, Sang-Jip,Kim, Seong Hwan,Kang, Heonjoong,Son, Young-Jin American Chemical Society and American Society of 2016 Journal of natural products Vol.79 No.7
<P>A new inhibitor, acredinone C (1), of receptor activator of nuclear factor-kappa B ligand (RANKL)-induced osteoclast differentiation was isolated from the culture broth of the fungus Acremonium sp. (F9A015) along with acredinones A (2) and B (3). The structure of acredinone C (1), which incorporates benzophenone and xanthone moieties, was established by the analyses of combined spectroscopic data including 1D and 2D NMR and MS. All of the acredinones studied efficiently inhibited the RANKL-induced formation of TRAP(+)-MNCs in a dose-dependent manner without any cytotoxicity up to 10 mu M. Acredinone A showed dual activity in both osteoclast and osteoblast differentiation in vitro and good efficacy in an animal disease model of bone formation.</P>
Antibacterial Butenolides from the Korean Tunicate <i>Pseudodistoma antinboja</i>
Wang, Weihong,Kim, Hiyoung,Nam, Sang-Jip,Rho, Boon Jo,Kang, Heonjoong American Chemical Society and American Society of 2012 Journal of natural products Vol.75 No.12
<P>Six new (<B>1</B>, <B>2</B>, and <B>5</B>–<B>8</B>) and three known (<B>3</B>, <B>4</B>, and <B>9</B>) butenolide metabolites were isolated from the tunicate <I>Pseudodistoma antinboja</I> by activity-guided fractionations. The structures were elucidated by combined NMR and MS spectroscopic methods. These compounds were evaluated for their antibacterial activity, and most of them exhibited moderate to significant activity that selectively targeted Gram-positive strains and did not exhibit cytotoxicity in the MTT assay at 100 μM. Cadiolides <B>5</B>–<B>9</B> in particular exhibited significant antibacterial activity that was comparable to or even better than those of marketed drugs such as vancomycin and linezolid against all of the drug-resistant strains tested.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2012/jnprdf.2012.75.issue-12/np300544a/production/images/medium/np-2012-00544a_0002.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np300544a'>ACS Electronic Supporting Info</A></P>
( Jihye Lee ),( Hiyoung Kim ),( Tae Gu Lee ),( Inho Yang ),( Dong Hwan Won ),( Hyukjae Choi ),( Sang Jip Nam ),( Heonjoong Kang ) 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0
Anmindenols A (1) and B (2), inhibitors of inducible nitric oxide synthase (iNOS), were isolated from a marine-derived bacterium Streptomyces sp. Their chemical structures were elucidated by interpreting various spectroscopic data, including IR, MS, and NMR. Anmindenols A and B are sesquiterpenoids possessing an indene moiety with five- and six-membered rings derived from isoprenyl units. The absolute configuration of C-4 in anmindenol B was determined by electronic circular dichroism (ECD) of a dimolybdenum complex. Anmindenols A (1) and B (2) inhibited nitric oxide production in stimulated RAW 264.7 macrophage cells with IC50 values of 23 and 19 μM, respectively.
( Dongyup Hahn ),( Hiyoung Kim ),( Inho Yang ),( Jungwook Chin ),( Hoosang Hwang ),( Dong Hwan Won ),( Byoungchan Lee ),( Sang Jip Nam ),( Merrick Ekins ),( Hyukjae Choi ),( Heonjoong Kang ) 영남대학교 약품개발연구소 2016 영남대학교 약품개발연구소 연구업적집 Vol.26 No.-
Three new structurally related depsipeptides, halieylindramides F-H (1-3), and two known halieylindra-mides were isolated from a Petrosia sp. marine sponge collected off the shore of Youngdeok-Gun, East Sea, Republic of Korea. Their planar structures were elucidated by extensive spectroscopic data analyses including ID and 2D NMR data as well as MS data. The absolute configurations of halicylin-drarnides F-H (1-3) were determined by Marfey``s method in combination with Edman degradation. The absolute config-urations at C-4 of the dioxyindolyl alanine (Dioia) residues of halieylindramides G (2) and H (3) were determined as 4S and 4R, respectively, based on ECD spectroscopy. The C-2 configurations of Dioia in 2 and 3 were speculated to both be 2R based on the shared biogenesis of the halicylindramides. Halieylindrarnides F (1), A (4), and C (5) showed human farnesoid X receptor (hFXR) antagonistic activities, but did not bind directly to hFXR.
Chang-Hun JI,Hiyoung KIM,Hyun-Woo JE,Hahk-Soo KANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Titer improvements of secondary metabolites using synthetic biology approach has been technically challenging if BGCs (biosynthetic gene clusters) contain large-size genes of multi-modular enzymes such as NRPS (non-ribosomal peptide synthetase) and PKS (polyketide synthase) due to the difficulty of gene synthesis. In such a case, top-down approach provides a viable alternative in which BGCs could be rationally engineered with the minimum input of synthetic DNAs. We successfully implemented this strategy for titer improvements of clinically important antibiotic daptomycin through stepwise transcriptional optimization of genes in its BGC, resulting in up to 1950% improvement in total lipopeptide titers compared to the wild-type strain. Upon decanoic acid feeding, daptomycin accounted for nearly half of total lipopeptide production. To the best of our knowledge, this is the highest improvement of daptomycin titer ever achieved through genetic engineering of S. roseosporus. The approach we describe here could be used as a general platform for the development of high titer industrial strains of secondary metabolites produced by BGCs containing large genes of multi-modular enzymes.