Gonadal Development, Spawning and Plasma Sex Steroid Levels of the Indoor Cultured Grunt, Hapalogenys nitens

Hee Woong Kang,Jae-Kwon Cho,Maeng-Hyun Son,Jong Youn Park,Chang Gi Hong,Jae Seung Chung,Ee-Yung Chung 한국발생생물학회 2015 발생과 생식 Vol.19 No.1

The gonadosomatic index (GSI), gonadal development and changes in hormones in plasma level of the indoor cultured grunt (Hapalogenys nitens) were investigated by histological study from August 2011 to October 2012. The GSI showed similar trends with gonad developmental stages during the culture periods. Changes in plasma level of estradiol-17β of female H. nitens reached the highest value before the spawning period, and seasonal changes in plasma level of estradiol-17β were similar in trends of oocyte developments and GSI changes. Testosterone levels of male H. nitens reached the highest value before and after the spent stage. Ovarian developmental stages of H. nitens could be classified into early growing stage, late growing stage, mature stage, ripe and spawning stage, recovery and resting stage. The testicular developmental stages could be divided into growing stage, mature stage, ripe and spent stage, and recovery and resting stage.

Gonadal Development, Spawning and Plasma Sex Steroid Levels of the Indoor Cultured Grunt, Hapalogenys nitens

Kang, Hee Woong,Cho, Jae-Kwon,Son, Maeng-Hyun,Park, Jong Youn,Hong, Chang Gi,Chung, Jae Seung,Chung, Ee-Yung The Korean Society of Developmental Biology 2015 발생과 생식 Vol.19 No.1

The gonadosomatic index (GSI), gonadal development and changes in hormones in plasma level of the indoor cultured grunt (Hapalogenys nitens) were investigated by histological study from August 2011 to October 2012. The GSI showed similar trends with gonad developmental stages during the culture periods. Changes in plasma level of estradiol-$17{\beta}$ of female H. nitens reached the highest value before the spawning period, and seasonal changes in plasma level of estradiol-$17{\beta}$ were similar in trends of oocyte developments and GSI changes. Testosterone levels of male H. nitens reached the highest value before and after the spent stage. Ovarian developmental stages of H. nitens could be classified into early growing stage, late growing stage, mature stage, ripe and spawning stage, recovery and resting stage. The testicular developmental stages could be divided into growing stage, mature stage, ripe and spent stage, and recovery and resting stage.

상피성 암세포주에 대한 녹차 Catechin의 효과

박정현(Jeong Hyun Park),김대중(Dae Joong Kim),한장희(Jang Hee Hahn),김홍태(Hong Tae Kim),정용욱(Yong Wook Jung),성훈기(Hoon Ki Sung),김주영(Joo Young Kim),송인환(In Hwan Song),성언기(Eon Gi Sung),이융창(Yung Chang Lee) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.5

Catechin은 녹차에서 추출되는 폴리페놀의 주요성분으로 고혈압 및 동맥경화의 예방효과, 당뇨억제효과, 항산화작용, 항암작용 등에 직접 관여하는 물질이다. 본 연구에서는 태평양에서 제공받은 녹차 catechin을 이용하여 상피성 암세포주인 A549 (폐암)와 EATC (복수암)세포에 투여한 후 처리농도와 시간에 따른 변화를 비교, 관찰함으로써 녹차의 효과와 작용기전을 밝혀내는데 목적이 있었다. 본실험은 A549 세포와 EATC 세포를 배양한 후 녹차 catechin을 1, 10, 100, 500 μg/ml의 농도로 48시간 동안 처리하였고, 광학현미경, 공초점현미경, 전자현미경 등을 이용하여 세포의 구조적 변화를 확인하였으며 MTT분석, 전기영동, 유세포분석기 등을 사용하여 세포 손상정도를 파악하였다. A549 세포에서는 catechin 1 μg/ml와 10 μg/ml 농도에서는 대조군에 비하여 큰 변화를 관찰할 수 없었다. 100 μg/ml catechin을 처리하였을 때 세포내의 검은 과립들의 수가 증가하였고 층판소체의 손상이 나타났다. 세포주기의 장애가 나타나 DNA 합성전기에 있는 세포들의 수가 급격히 증가하였다. 500 μg/ml 농도에서는 층판소체와 사립체의 파괴가 심하게 나타났으며 세포생존율이 감소하였고 세포주기의 장애도 관찰되었다. EATC 세포에서는 catechin의 농도가 A549 세포의 경우보다 낮은 농도에서도 세포증식 억제 및 세포손상 효과가 나타났다. 10 μg/ml 농도에서도 세포의 위축과 생존율의 감소가 일어났으며 전기영동상에 괴사되는 세포들이 파악되었다. 100μg/ml catechin을 처리하였을 때 자연사의 형태학적 관찰, 전기영동, 유세포분석 등에서 자연사 과정에 있는 세포들이 많이 나타났다. 결과적으로 녹차 catechin을 배양한 상피성 암세포에 투여함으로서 세포의 생존율과 증식이 억제되었고 그 과정에서 괴사, 자연사, 세포주기의 장애 등이 관여하는 것으로 생각된다. 그러나 일련의 세포손상과정을 유도하는 데 있어 세포의 종류, 처리 시간, 농도에 따라 다소 차이가 있음을 확인하였다. Catechin is main component of polyphenol extracts from green tea, it is associated with prevention of hypertension and atherosclerosis, anti-diabetic effect, antioxidant, antitumor. The purpose of this research is to investigate the effect and its mechanism of green tea catechin on epithelial cancer cell lines in various concentrations and durations. For this study, epithelial cancer cell lines, A549 (lung cancer), EATC (Ehrlich-Lettre ascites tumor cell) were used. Inverted, light, confocal and electron microscopes were applied to find morphological changes. MTT assay, flowcytometric analysis, gel electrophoresis were used to compare severity of cellular damages to control after exposure to 1, 10, 100 and 500 μg/ml catechin for 48 hours. In the A549 cells, after 1 μg/ml and 10 μg/ml catechin treatments, there was no notable changes. However, exposure to 100 μg/ml catechin induced increase of cytoplasmic granules, destruction of lamellar body, inhibition of cell cycle, especially G0/G1. In the early phase of 500 μg/ml catechin administration, decrease of cell population, severe destruction of lamellar bodies and mitochondria, derangement of cell cycle were shown. In the EATC, such as those effects occurred after exposure to lower concentration of catechin than in that of A549 cells. After exposure of 10 μg/ml catechin, rounded-up cells and necrotic cells were found. Whereas, most of cells were under apoptotic changes-cytoplasmic condensation, nuclear fragmentation, cellular shrinkage, ladder pattern in the electrophoresis, when administrated 100 μg/ml catechin. These results suggested that exposure of catechin induced severe cellular damage and growth inhibition in dose- and time-dependent manner. And we confirmed that these effects of catechin were involved with apoptosis, necrosis and cell cycle arrest and were quite different according to cancer type. Therefore, much more research would be demanded before clinical application of catechin to human cancer therapy and this study would be the basic source for further study of green tea.

쥐노래미 Hexagrammos otakii 난의 부화에 미치는 수온의 영향 및 자치어의 성장과 생존에 미치는 수온과 먹이의 영향

강희웅,정의영,이창훈,이정열 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.2

쥐노래미 Hexagrammos otakii의 부호조건 및 자치어의 성장 및 생존에 미치는 영향을 조사하기 위하여 수온에 따른 부화 소요시간 및 부화율, 자치어의 성장과 생존에 미치는 수온과 먹이조건을 조사하였다. 쥐노래미 수정란의 수온별 부화 소요시간은 수온이 높을수록 짧았다. 부화율은 12℃에서 가장 높았으나, 반면 수온이 14℃ 이상으로 상승하면 급격히 감소되었으며 18.0℃이상에서는 비정상 기형 개체들이 출현하였다. 부화 후 70일 경과 후 수온별 자치어의 초기 성장과 생존율(%)은 수온이 높은 조건(16℃)일수록 낮은 수온조건보다 성장이 빨랐던(P<0.01) 반면, 생존율은 낮은 수온(10℃)조건에서 높게 나타났다. 70일간 먹이공급계열이 따른 자치어 사육실험에서 최적 성장과 높은 생존 조건은 윤충류(Brachionus plicatilis, 부화 후 1~10일) + Artemia nauplii(부화 후 10~20일) + 배합사료(부화 후 15~70일)를 공급한 실험군으로 나타났다. 자치어의 빠른 성장과 생존율을 높이기 위해서는 되도록 일찍 배합사료에 먹이붙임을 하는 것이 바람직하다고 판단된다. To obtain optimal rearing conditions for seedling production of the greenling Hexagrammos otakii, the effects of seawater temperature on hatching of egg, and seawater temperature and feeding regime on growth and survival of hatched larvae were studied. The hatching time of fertilized eggs of H. otakii decreased with increasing seawater temperature. Total length of hatched larvae was in the range of 7.21~8.16㎜. The highest hatching rate was found at 12℃. As seawater temperature increased higher than 14℃, the hatching rate sharply decreased. Abnormal larvae were found at 18.0℃. Seawater temperature affected significantly the growth and survival of H. otakii larvae(P<0.001). After rearing larvae for 70 days after hatching, we found the growth rate highest when seawater temperature was highest(16℃), whereas survival was highest when seawater temperature was lowest(10℃). Feeding regime also affected significantly the growth and survival of H. otakii larvae(P<0.001). The most efficient feeding regime was under the conditions of feeding rotifer(Brachionus plicatilis) during days 1~10 after hatching+ Artemia nauplii during days 10~20 after hatching+commercial feeds during days 15~70 after hatching. To ensure rapid growth and higher survival of H. otakii larvae, commercial feeds should be provided as early as possible.

主要日刊新聞 家庭欄의 分析과 主婦의 反應

趙昌淑,金敎玉,金暎辰,成玉姬 건국대학교 1974 學術誌 Vol.17 No.1

The purpose of this study is to investigate the degree of the interest of several newspapers in the household management and to analyse the actual need of housewives, the reader of the newspapers, for such informations given by the newspapers. 1305 articles on any subjects relating household management are gathered from 6 major newspapers published in Seoul during the year 1972, from January to December and the answers of 1080 housewives living in Seoul to the Specially prepared questionaire are collected for this purpose. The contents of articles are discussed under the classification of 6 cathegories: clothing, food, housing, child rearing and health, family relationships and home management. All newspapers revealed similar tendencies in providing the family column. The discrepancy between the actual need of the housewives and what has been offered by newspapers was found in every cathegories of the article. The erroneous, inadequate informations are pointed out particularly those of designs and technics of foreign dress making and also the food preparation, whereas well elaborated articles could be also recognized in the field of child rearing and the health. The notable thing was the lack of the interest from the side of newspapers partially also of the housewives in the method of making traditional costume and food, and in the matter of traditional etiquette. For the most of housewives use mainly the newspaper as the resource of their knowledge about the household management the important role of newspaper and the necessity of specialist's supervision for the family column were strongly emphasized by the authors.

先天性 心臟畸形의 外科治驗例 : 34例 報告 A Report of 34 Cases

元官喜,李亨默,徐景弼,鄭埈,李東植,洪彰義,洪基元,李寧均 醫藥係社 1963 綜合 醫學 Vol.8 No.1

활로四徵의 姑息的 手術 : 16例 報告 A Reprot oof 16Cases

元官喜,李亨珪,洪彰義,李華永,李寧均 醫藥係社 1963 綜合 醫學 Vol.8 No.10

단백체를 이용한 애기장대 Cytokinin 유도 단백질의 분석

양영실,차준영,네티 엘마와티,정민희,배동원,이창원,손대영 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching. We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.

Oral Presentation : Improvement of the peri-implant site through hard tissue augmentation

( Hee Yung Chang ),( Bong Jin Kim ),( Seong Nyum Jeong ),( Yun Sang Kim ),( Sung Hee Pi ),( Hyung Keun You ),( Hyung Shik Shin ) 대한치주과학회 2010 대한치주과학회 학술대회자료집 Vol.2010 No.1

Seasonal Characteristics of Fecal Sites of the Siberian Flying Squirrel Pteromys volans

Chang Wook Han,Sang Jin Lim,Hee Bok Park,Yung Chul Park 강원대학교 산림과학연구소 2018 Journal of Forest Science Vol.34 No.2

Characteristics of fecal sites of the Siberian flying squirrel Pteromys volans was analyzed based on 132 sites of total 19 places. The fecal sites were more frequently found in winter (43.9%), and then followed by autumn (27.3%), spring (23.5%), and summer (5.3%). With the exception of summer, the fecal sites were more frequently found at the root collar than on the forked tree (p<0.01). Among 132 fecal sites, 88 sites (66.7%) were found on the rood collars and the other 44 sites (33.3%) were posited in the forked trees. Brown or red clay pellets were found at 44 fecal sites (33.4%) and 43 fecal sites (32.6%), and then black and yellow pellets were at 22 fecal sites (16.7%) and 19 fecal sites (14.3%), respectively. Green pellets were rarely found only at 4 sites (3.0%). Feces tend to have bright colors (brown, red clay and yellow) in winter and black in summer. Fecal sites with yellow pellets were much less found in all of the three seasons with the exception of winter, but highly increased in 25.4% in winter. The fecal sites with brown (33.4%) and red clay pellets (32.6%) were most frequently found through the four seasons.