한방 이화주의 미백 및 피부 주름 개선 효과

이상진(Sang-Jin Lee),권이영(Yi-Young Kwon),조성원(Sung-Won Cho),권희숙(Hee-Suk Kwon),신우창(Woo-Chang Shin) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.4

본 연구에서는 한방이화주의 피부 생리기능 활성을 알아보기 위해 70% EtOH 추출물의 피부 미백, 주름 개선 및 항염증 효과를 조사하였다. HEE은 tyrosinase 활성 억제 및 tyrosine을 기질로 melanin이 형성되는 pathway에 관여하는 주요한 인자인 TRP-1과 TRP-2를 저해하는 작용 기전을 통해 피부 색소침착의 주요 원인 물질인 melanin 생합성을 농도 의존적으로 저해하는 것을 확인하였다. HEE은 피부진피 내 피부 탄력을 유지하는 elastin을 분해하는 효소인 elastase의 활성을 저해하였고, 피부의 keratinocyte가 생성분비하는 MMP-2와 MMP-9의 단백질 발현과 proteolytic 활성을 억제하여 노화에 따른 피부 주름 생성 억제할 수 있는 가능성을 확인하였다. 또한 세포 독성 없이 LPS에 의해 유도된 염증 반응을 50% 저해하는 HEE의 농도(IC50)는 24.9 μg/mL이며, 50 μg/mL 농도로 처리하였을 때 염증 반응 저해 효과가 70%로 높은 효과를 가지는 것을 확인하였다. 이상의 결과를 종합하면 HEE의 피부 미백, 주름 개선 및 항염증에 우수한 효과를 나타내고 있으므로 기능성 화장품의 주요한 소재로 이용 가치가 높을 것으로 사료된다. Extrinsic skin aging is characterized by the loss of skin tone and resilience, irregular pigmentation, and deep wrinkles. The aim of this study was to investigate the effects of Ehwa Makgeolli containing oriental herbs (Glycyrrhiza uralensis Fisch., Lycium chinense MILL., Morus alba L., and Saururus chinensis Baill) on skin whitening and wrinkling in human skin cells. We prepared Makgeolli extracts (HEE) with 70% ethanol. HEE significantly inhibited in vitro mushroom tyrosinase activity and reduced the cellular and secreted melanin content of mouse melanoma melanocytes (B16F1 cells). HEE down-regulated the protein expression of tyrosinase related protein (TRP)-1/-2, a key player in melanogenesis. Treatment with HEE in human keratinoctyes (HaCaT cells) inhibited the proteolytic activities of matrix metalloproteinase (MMP)-2/-9 in a dose-dependent manner and dramatically reduced the expression of MMP-2/-9. In addition, HEE attenuated lipopolysaccharide (LPS)- induced nitric oxide production in murine macrophages (RAW264.7 cells). These results indicate that HEE may be a great cosmeceutical ingredient for its whitening, anti-wrinkle, and anti-inflammatory effects.

韓國人에서 多重增幅 重合酵素反應으로 分析한 STRs 遺傳座位의 遺傳的 多樣性

권국환,이혜린,박종진,황적준,이희석,송은섭 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

The tetrameric STRs loci were studied in a population of Korean(n=223) for allele frequency distribution and applicability to identity and paternity testing, using multiplex PCR with electrophoresis of the PCR products in DNA sequencing gels and subsequent detection of allelic fragments by silver staining. The STRs loci analyzed were HUMCSF1PO, HUMTPOX, and HUMTHO1 ; in the Korean population, 9 alleles with their frequency range of 0.002-0.363 are detected in the HUMCSF1PO, 5 alleles with those of 0.020-1.480 in the HUMTPOX, and 6 alleles with those of 0.002-0.489 in the HUMTHO1. The highest observed heterozygosity is found at the locus HUMCSF1PO(0.709), those of the loci HUMTPOX and HUMTHO1 being 0.646 and 0.632, respectively. All loci meet Hardy-Weinberg expectations ; there are good agreement between the observed and expected values under Hardy-Weinberg equilibrium. Moreover, pairwise comparisons between loci show allelic independence for all the 3 loci comparisons. The power of discrimination (PD) determined for the locus HUMCSF1PO is 0.8896 (88.96%), that of the HUMTPOX is 0.8818(88.18%), and that for the HUMTHO1 is 0.8367(83.67%) ; the combined power of discrimination for the triplex is 0.9976(99.76%). The power of exclusion(PEX) calculated for the loci HUMCSF1PO, HUMTPOX, and HUMTHO1, being prior probability that a falsely accused father will be excluded, and 0.5173(51.73%), 0.4610(46.10%), and 0.5101(510.01%), respectively, and the cummulative power of exclusion(CPE) for all the three loci is 0.8726(87.26%). Thus, these allelic frequency data can be used to construct the database of the multiplex PCR-based DNA profile in the Korean population. The calculated parameter, "power of discrimination(PD)" and "power of exclusion(PEX)", show the informativeness of these loci for the determination of identity and relatedness of individuals.

청국장의 암예방 증진 연구 : 발효기간과 부재료의 양을 달리하여 제조한 청국장의 항돌연변이 효과 Antimutagenic Activity of Chungkookjangs Prepared with the Different Fermentation Periods and Ingredient Ratio

권은영,정근옥,문숙희,박건영 대한암예방학회 2002 Journal of cancer prevention Vol.7 No.3

제지폐수처리용 생물고분자응집제를 생산하는 Bacillus sp. K-111의 배양특성조사

권기석,손용호,최선택,정석관,송숙희,김동걸 7개 국립대학교 환경연구 논문집 공동발행 위원회 2002 환경연구논문집 Vol.2 No.1

Flocculant-producing microorganisms were screened from obtained strains in the laboratory using a pulp-wastewater treatment as the flocculating agent. K-111 strain that had high flocculating activity among them was selected and identified as Bacillus sp. K-111 16s rDNA sequencing. The favorable medium for the production of flocculant was glucose 1.5%, NH_4NO_3 0.2%, tryptone 0.01%, K_2HPO_4 0.08%, KH_2PO_4 0.06%, CaCO_3 0.03%, MgSO_4·7H_2O 0.005%, MnSO_4 0.005% in 1 liter of D.W. at initial pH 7.0. The optimum culture temperature and pH were 30℃ and pH 7.0, respectively. the flocculating activity was observed most highly after 36 to 48 hr of cultivation at the optimum conditions. the flocculating activity of produced biopolymer on optimum conditions was about 2.5-fold higher than that of screening medium.

난소에 발생한 원발성 소세포암종 : 2예 보고 Two cases reports

권계원,고은석,김희경 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Primary small cell carcinoma of the ovary is a rare, aggressive tumor of young women and has two subtypes. The pulmonary type small cell carcinomas of the ovary that resembled small cell carcinoma of the lung and differed from both clinically and pathologically from small cell carcinoma of the ovary, usually associated with hypercalcemia. The histogenesis of the primary ovarian small cell carcinoma has not been established. We have identified two cases of small cell carcinomas primary in the ovary. One has microscopic features of pulmonary type, and the other has hypercalcemic type. Pulmonary type small cell carcinoma patient was a 40-year-old woman. She was admitted due to known ovarian mass. She was well before this episode. Total abdominal hysterectomy and bilateral salpingo-oophorectomy was performed. Gross examination revealed 15cm sized right ovarian mass which was mostly solid with multifocal necrosis. Histologically, the tumor was composed of small to medium sized round cells with scanty cytoplasm, hyperchromatic nuclei, inconspicuous nucleoli, numerous mitotic figures forming large sheets and diffuse pattern. Hypercalcemic type small cell carcinoma patient was a 43-year-old woman. She was also complained of right abdominal swelling and discomfort of 1 month duration. She was received total abdominal hysterectomy due to myoma in 3 years ago. The serum calcium levels was elevated. Right salpingo-oophorectomy and omentectomy was performed. Gross examination revealed 15cm sized huge ovarian mass which was mainly solid with small cystic components and hemorrhagic necrosis. The omentum showed several oval shaped metastatic nodules. Microscopic examination reveals closely packed tumor cells with scanty cytoplasm, vesicular nuclei that contains single nucleoli, and numerous mitotic figures. Many folliculoid spaces are present. Pulmonary type small cell carcinoma patient died 13 months after surgery. Hypercalcemic type small cell carcinoma patient was alive without evidence of disease 2 months after surgery but lost to follow up thereafter.

폐흡충증 1예

고은석,권계원,김희경 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Pulmonary paragonimiasis is one of the most important endemic parasite disease in Korea despite decreased prevalence recently which clinically manifest cough, hemoptysis and chest pain, and radiologically necessitate differential diagnosis with tuberculosis or lung cancer. We experienced a case of pulmonary paragonimiasis in a 64-year-old female with cough and intermittent hemoptysis. A thoracic computed tomography (CT) scan revealed variable sized nodules with central necrosis suggesting chronic organizing pneumonia with abscess formation of fungus granuloma. Histopathologically, necrotizing granulomatous inflammation and egg granulomas were prominent and the surrounding lung parenchyma showed chronic organizing pneumonia.

자궁경부암 병기판정 기초검사시 새로운 보조방법으로서 고주파 질식 초음파의 가치

유희석,이은주,장기홍,권혁찬,양정인,김행수,오기석 아주대학교 의과학연구소 1996 아주의학 Vol.1 No.1

To investigate the efficacy of endoluminal ultrasound system (EUS) as a surrogate for high frequency transvaginal ultrasonography and as an optional baseline study in determining parameirial and stromal invasion of early stage cervical cancer, 52 women suspected of cervical cancer underwent EUS. A 12 MHz endoscopic probe was employed to radially scan the cervix for possible lesions suspected to be invasive cancer during a period of 6 months from Feb. 1 to July 1, 1995. Patients also underwent magnetic resonance imaging (MRI) and/or computerized tomography (CT) as a routine mode of baseline study, and were clinically staged by 3 independent physicians specializing in Obstetrics and Gynecology at the Department of Obstetrics and Gynecology, School of Medicine, Ajou University, Suwon, Korea. Thirty one patients subsequently received surgery appropriate for the stage of the disease and the final pathology findings were compared with the results of clinical staging, EUS, MRI/CT by regression analysis. The results showed that there was statistically significant correlation between MRI/CT and pathology (r=0.660, p<0.02), between EUS and pathology (r=0.803, p<0.01), and between clinical staging and pathology (r=0.825, p<0.01). It is concluded that there was significant statistical correlation t>etween EUS, MRI/CT, clinical staging and pathology, but the question remained as to the statistical superiority of EUS over MRI/CT with regard to parametrial invasion and stromal invasion depth assessment. Finally, EUS is useful as an alternative optional diagnostic tool in the baseline study of cervical cancer.

醫藥分業에 대한 保健所 公務員들의 認識과 態度

박석수,박재용,권진희 경산대학교 보건복지연구소 2000 保健福祉硏究 Vol.5 No.-

This research was conducted to find the attitude of public health officials about the division of roles between doctors and pharmacists (DRDP) which would be committed from July 01, 2000. The survey was carried out for the 600 public health officials from 25 health centers between March 2, 2000 and March 31, 2000 in Kyoungsan-Bukdo area. 33.0% of respondents were highly aware of the DRDP, and 63.0% were moderately aware of the DRDP. The proportions of the respondents who recognized that the health center should include DRDP agency are 43.3% among the health officials, 25.8% among the administrative officials, 80.0% among the drug relating officials, and 60.0% among the temporary officials. 65.3% of respondents answered that exceptions of DRDP should be allowed in some cases, such as, in areas where there is no medical facilities or pharmacies. 58.0% responded that the network system between pharmacies and medical facilities should be necessary so that the exchange of information is allowable in case that the prescribed medicine is not in the pharmacies. 30.0% agreed that the abuse of injections should be prevented, and 41.2% responded that the health center should emphasize public relations when there is any inconvenience to implement the injection. 65.3% were that there is little inconvenience about substitution of preparation of medicine, and 28.2% were that the strengthening of the administrative action by the pharmaceutical affairs law might be useful in order to improve patients convenience. The participants supported that a doctor can prepare patients medicine for an emergency patient (97.7%) and the first infectious disease (96.5%). The participants also supported that a pharmacist can prepare patients medicine for the areas of no medical facilities (79.3%) and disaster areas (78.8%). The participants were affirmatively showed about the system of using community drug sotre (helpful (66.5 percent) and not helpful (33.5 percent)) and the fuction of the primary medical institutions, that is, the family doctor system (helpful (81.8%) and not helpful (18.2%). The participants also recognized that the regional cooperation committees would be helpful to minimize patients inconvenience. Officials participated in this survey responded that education of residents and the public information by consumers cooperative institution would produce the most efficient effect. This study can be concluded that the recognition of health officials varies by the education level, job position level, and primary duty. Therefore, the education of DRDP for health officials is necessary for improvement of their ability and knowledge and for the smooth introduction of the division between medical roles.

중등 과학교사 양성 교육과정과 교수내용지식 연구 동향의 탐색

조희형,조영신,권석민,박대식,강영진,김희경,고영자 이화여자대학교 교과교육연구소 2006 교과교육학연구 Vol.10 No.2

과학교사의 양성을 위한 교육과정 또는 그 프로그램의 주된 목적은 바람직한 과학교사가 지녀야 할 전문적 자질을 함양시키는 데에 있다. 그러나 우리나라의 과학교사 양성을 위한 프로그램 또는 사범대학 과학교육학 계열 교육과정에는 과학교사 교육의 본성과 그의 자질에 관한 연구의 결과가 충분히 반영되어 있지 않다. 이 연구는 자질기반 과학교사 양성 교육과정의 개발 틀, 교직의 전문성과 전문성발달기준, 우리나라의 과학교사 양성 교육과정 및 프로그램에 관한 문헌을 조사·분석한 다음, 그 결과로부터 우리나라의 과학교사 양성을 위한 교육과정의 개발 방향을 도출하고 그 모형을 탐색할 목적에서 수행하였다. 이 연구에서는 과학교사 양성 교육과정을 그 과목이나 지식기반 영역에 따라 교수내용알기(PCKg), 교수구성단위(TPCs), 내용표상화(CoRe)와 교수전문경험자료(PaP-eRs), 그리고 교수상황지식(PCxK)을 단위로 이용하여 구성할 것을 강조하였다. 또한 과학교사의 전문적 자질의 함양에 목적을 둔 교육과정 또는 그 프로그램의 과학교육 및 과학과 관련된 과목으로 일반과학, 심화과학, 일반과학교육학(과학교육론, 과학 교수-학습 포함), 과학 교육과정 및 평가, 과학교육 시설과 자원, 과학교육 실습, 과학교육 연구를 제안하였다. The subjects and their contents comprising the curricula for secondary science teacher education should be selected/organized to assist pre-service teachers to gain understandings of knowledge ba ses and to help them acquire professional teaching competences. However, the nature of science teacher education has not been reflected on the Korean science teacher education programs. The research was performed to review the literature on teachers' competencies including PCK(pedagogical content knowledge), teaching as profession, curricula or programs for secondary science teacher education, and, based on the results, to suggest curricular subjects and their contents for secondary science teacher education in Korea. The research emphasizes that the subjects and their contents for secondary science teacher education should be developed by the use of PCKg(pedagogical content knowing), TPCs(teacher professional constructions), CoRe(content representation) and PaP-eRs(pedagogical and professional-experience repertoires) as constitutive units, depending on the subject areas or contents. Also suggested in this research are the knowledge bases assumed to be necessary for the development of secondary science teachers' professional qualities, which include general sciences, enrichment sciences, general science education, science education curricula and evaluation, facilities and resources for science education, teaching practices, research in science education.

멀티미디어 디스플레이-시간 합성 모델 : 기업 홍보시스템에의 적용 An Application to a Company Advertizing System

왕숙희,백두권 한국경영과학회 1994 韓國經營科學會誌 Vol.19 No.1

An important modeling requirement for multimedia systems is the representation of temporal constraints among several different media that ordinarily arise in multimedia application. The multimedia system which integrates various types of data is required to express voice, image, text and graphic data with simultaneousness or an arbitrary time intervals. The purpose of this study is to analyze the temporal reationships and synchronization among several different kinds of medium. Moreover, it proposes a Multimedia Display-Time Composition Model(MDTC Model) which guarantees a representation of any kind of temporal synchronization among more than two kinds of medium. The proposed model is applied to a company advertizing system. Finally, a multimedia authoring tool based on the MDTC Model is implemented.