배지의 무기염류와 sucrose 농도가 감자의 기내 shoot 증식과 기내경 삽목묘의 생존율에 미치는 영향

김희곤,임순희,안장순,송창길,박미경,강봉균 全南大學校 農業科學技術硏究所 1999 農業科學技術硏究 Vol.34 No.-

감자의 virus-free 기내경 삽목묘의 효율적인 대량 생산 체계의 확립을 위하여 생장점 배양으로 얻어진 감자의 기내경의 증식과 기내경의 순화삽목을 실시한 결과는 다음과 같다. 초대배양에서는 염류농도와 sucrose 농도가 높을수록 양호하였다. 즉, MS나 2MS에 sucrose를 6% 첨가한 배지에서 튼튼한 기내경이 생산되었다. 계대배양에서는 sucrose 농도가 높을수록 줄기의 길이는 단축되고 굵기는 커졌으며 생체중은 염류농도와 관계없이 3%의 sucrose가 첨가된 배지에서 무거웠다. 이들 유식물체의 경절을 삽목한 결과 계대배양 배지의 sucrose농도가 6%인 배지에서 생존율이 높은 경향이었다. In order to establish a mass propagation system of potato planting material, in vitro multiplication of virus-free shoot originated from meristem culture and sucrose concentrations on survival of cutting of the in vitro stem tried with various media containing different levels of MS salts(0.5x, 1x and 2x) and sucrose(1.0, 3.0, 6.0%), and the effects of MS salts elements were evaluated. Higher level of sucrose in the initial culture media favored the shoot growth, that is, both of 1MS and 2MS media containing 6.0% sugar produced stouter shoots. Higher level of sucrose in the subculture media also favored the development of compact stout shoots, but biomass production in terms of fresh weight was high on the media containing 3.0%sucrose, regardless of MS salts concentration. The survival rate of cuttings made of the in vitro stem segments tended to be higher in those obtained from subculture media containg 6.0%sucrose.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

소의 대동맥 내피세포에서 산화저밀도 지단백에 의한 아포프토시스에 백일해 독소 및 콜레라 독소가 미치는 영향

손호영(Ho Young Son),강성구(Sung Ku Kang),강무일(Moo Il Kang),차봉연(Bong Youn Cha),이광우(Kwang Woo Lee),장상아(Sang Ah Chang),최윤희(Yoon Hee Choi),문성대(Sung Dae Moon),안유배(Yoo Bae Ahn),손현식(Hyun Sik Son),윤건호(Kun Ho Yoon) 대한내과학회 1999 대한내과학회지 Vol.56 No.5

N/A Objectives : Oxidized LDL is thought to play a key role in atherogenesis. Among their wide variety of biological properties, oxidized LDL injures the endothelium as an early event of atherogenesis. However, the mechanisms by which oxidized LDL injures endothelial cells are not definitely known. In order to evaluate the involvement of GTP-binding protein on the mechanism of apoptosis, we studied the effects of pertussis and cholera toxin on oxidized LDL-induced apoptosis in bovine aortic endothelial cells(BAECs). Methods : Oxidized LDL elicited apoptosis in cultured BAECs as shown by characteristic morphological and biochemical changes. Chromatin condensation and nucleus fragmentation were visualized by using fluorescence microscopy of intact cells staining by acridine orange/ ethidium bromide. DNA fragmentation was quantified by an ELISA with specific antibody for bromodeoxyuridine- labelled DNA fragments and confirmed with DNA ladder formation. Results : Studies using a combination of bacterial toxins which change the function of GTP-binding protein suggest that oxidized LDL-induced apoptosis was regulated by GTP-binding protein. Oxidized LDL-induced apoptosis was not changed by pretreatment of BAECs with pertussis toxin. In contrast, pretreatment with cholera toxin completely prevented the oxidized LDL- induced apoptosis. Conclusion : These results show that oxidized LDL induces apoptosis of BAECs and suggest that cholera toxin-sensitive G-proteins are involved in signal transduction of oxidized LDL-induced apoptosis of BAEC.

소아에서의 미세수술에 의한 재건술

안희창,전명곤,김정철 大韓成形外科學會 1999 Archives of Plastic Surgery Vol.26 No.3

Microsurgical reconstruction is necessary for children to correct severe trauma and congenital or acuqired deformity. The aim of this study was to evaluate whether or not microsurgical reconstruction is a safe and reliable operation in children and to analyze the differences of microsurgical reconstruction in children compared to adults. The study included 12 children who underwent 13 microsurgical reconstructions among a total of 251 cases of microsurgical reconstruction from May, 1986 to August, 1998. Their ages ranged from 24 months to 14 years and 8 months. There were 7 males and 6 females. The involved sites were 9 legs, 3 hands and 1 face. The causes of microsurgical reconstruction were 9 traumas, 2 congenital anomalies, 1 acquired deformity and 1 cancer. The applied flaps were 4 scapular flaps, 2 rectus abdominis muscle flaps, 1 de-epithelized groin flap, 1 lateral arm flap, 1 forearm tendocutaneous flap, 1 forearm tendocutaneous flap, 1 latissimus dorsi muscle flap, 1 fibula flap, 1 second toe transfer, and 1 wrap-around flap. All patients have had normal growth of the donor and recipient sites without specific complications during an average 2 years follow-up. We concluded that microvascular reconstruction is a very useful and reliable procedure in children if it is performed in consideration of each child's specific characteristics and conditions.

여성 외음부에 발생한 선상 편평상피세포암종

김창옥,양기화,강석진,이안희,김병기,김선무 대한병리학회 1991 Journal of Pathology and Translational Medicine Vol.25 No.1

韓國의 地震 特性과 液狀化判定에 關한 考察

安鍾弼,沈泰燮,朴昌熙 조선대학교 국토개발연구소 1987 국토개발연구 Vol.7 No.1

In thesis, I research and analyze an earthquake phenomenon in the Korean Peninsula on the basic of historical records and observational data, and define its characteristics. Researching a liquefactive judgements method in the united states of American, Japan and China, I compare it with that method in Korea. Considering the enlargement tendency of the building and earthquake activity in the Korean Peninsula, when we design the general structure in our country we have to review an earthquake-proof in building. It is very fortunate that the soil liquefaction judgement criteria was legisterved recently. Considering the characteristics of earthquake in Korea, the judgement criteria of soil liquefaction have to be different from that of in Japan or the United States of America. So I presented here an amended equation that is following. τ_d/σ_υ^1=0.33σ_υ/σ_υ^1ㆍrd The constant 0.33 in the above relation, which reflects the earthquake characteristics of the Korean Peninsula to some degree, appears resonable compared to those of other areas of the world.

Shock로 發生한 成人 Respiratory Distress Syndrome

安喜淳,張連珍,金仁世,趙裕永,金晟烈 순천향의과대학 1978 Journal of Soonchunhyang Medical Science Vol.1 No.1

The adult respiratory distress syndnome was originated from acute hemorhagic and septic shock in 3 patients after operation. All patients recovered with active management (PEEP,albumin,diuretics,and steroid) and various intensive therapies. Etio-logical, Pathophysiological and therapeutic points in A.R.D.S. were discussed with review of articles.

뇌성마비아의 모발내 중금속 함량 분석

안경희,김희상,유창현,윤동환,홍장혁,유승돈,이준원 경희대학교 2001 慶熙醫學 Vol.17 No.1

삼출성 중이염 환자에서의 혈중 및 아데노이드 면역글로불린 E의 면역혈청학적 연구

장혁순,강성호,유창수,김성현,이병돈,박영진,윤세동,안희영,강주원 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Otitis media with effusion (OME) is one of the most common disease in children but its pathogenesis is not clear. In particular the role of allergy has revealed conflicting support with widely varing opinions as to its importance. Within recent years, striking biochemical and immunochemical analytic techniques have opened a new approach the study of otitis media with effusion. So we focused on distributions of IgE in adenoid tissue and serum IgE levels. Authors compared the serum IgE and the distribution of IgE in adenoid tissue between the 20 patients underwent tonsillectomy, adenoidectomy and ventilating tube insertion and control group of 11 patients underwent tonsillectomy and adenoidectomy. The results were as follows; 1. In the distribution of IgE in adenoid tissue, there was a significant increase in the group of patients with OME compared to the control group (P<0.01). 2. In the analysis of serum IgE levels, the levels of IgE in the OME patients an control group were higher than normal level, although the level of IgE in the OME patients were less than that of control group, there was no significance P>0.05).

흰쥐 이자 Insulin과 Neuropeptide Y 분비세포의 발생에 관한 면역조직화학적 연구

장인엽,박태우,정윤영,김종중,문정석,김영택,안계훈,김남훈,선희매 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

Cells immunoreactive to insulin(INS-IR) and neuropeptide Y(NPY-IR) were found in prenatal(E15, 17, 19) and postnatal(P0, 5, 10, 15, 30, adult) rat pancreas using immunohistochemistry. Pancreatic INS-IR and NPY-IR were detected by day E17. They were found initially in the interlobular pancreatic duct, intralobular pancreatic duct and pancreatic islet gradually. Especially, INS-IR was detected in the dorsal pancreatic bud by day E19. The INS-IR and NPY-IR patterns were nearly similiar to each other, and many cells revealed colocalization of INS and NPY until day P10. But there was a change between them by the day P15 ; central INS-IR cells and peripheral NPY-IR in the pancreatic islet. The number of pancreatic islets per square millimeter of pancreatic tissue were decreased, and the number of immunoreactive endocrine cells per islet was increased according to development. These results suggest NPY may play a role in the development of the pancreatic endocrine cells.