The Activity of Alcohol Dehydrogenase and Acetaldehyde Dehydrogenase of Red Ginseng Mixture Drink and the Hepatoprotective Effect on HepG2 Cells

Han-Eul Yang,Ye Jeong Jeon,Choong Berm Park,Bo Hee Park 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

This study was conducted to investigate the activity of alcohol dehydrogenase(ADH) and acetaldehyde dehydrogenase(ALDH) of Red ginseng mixture drink(RGD) and the hepatoprotective effect of RGD on ethanol-treated HepG2 cells. The activities of ADH and ALDH was measured by ethanol assay kit and acetaldehyde kit(Megazyme) and cytotoxicity and the hepatoprotective effect were measured by MTT assay in HepG2 cells. As a result, the toxicity was not observed and the hepatoprotective effect of Red ginseng(RG), Hovenia Dulcis Thunb(HD), Radix Puerariae (RP), RGD, product A, and product B was 54.07%, 64.06%, 56.61%, 78.69%, 75.45%, and 76.69%, respectively, with the highest RGD. Furthermore, the activity of ADH of RG, HD, RP, RGD, product A, and product B was 111.16%, 100.24%, 118.29%, 383.14%, 155,58%, and 152.49% respectively, indicating that RGD was approximately 2.5 times higher. However there were no difference in ALDH activities. These results confirmed ADH and ALDH activities and hepatoprotective effects of RGD and it is thought that RGD was showed higher ADH activity and hepatoprotective effect than single extracts and commercial products.

A Safety Survey of Pesticide Residues in Quality Certified Products of Jinan Red Ginseng

Han Eul Yang,Ji Won Lee,Seung Mi Hwang,Tae-Young Kim 한국분석과학회 2023 학술대회논문집 Vol.2023 No.11

A Safety Survey of Pesticide Residues in Quality Certified Products of Jinan Red Ginseng

Han Eul Yang,Ji Won Lee,Soo Chang Na,Da Eun Lee,Jong Hee Yoo,Seung Mi Hwang,Tae-Young Kim 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

우리나라의 食品 및 營養素 攝取의 變化에 對한 考察

한양일(Yang Il Han),김을상(Eul Sang Kim),이규한(Kyu Han Lee) 한국식품영양과학회 1983 한국식품영양과학회지 Vol.12 No.2

1970年代의 우리나라 國民의 食品 및 營養素攝取의 變化에 對한 考察을 실시하기 위하여 國民總生産量, 國民所得, 食品攝取 및 營養素攝取狀態를 調査한 바 다음과 같은 結論을 얻었다.<br/> 1. 우리나라의 人口의 10年間 平均增加率은 1.7%였고 國民總生産量은 每年 平均 9.62%의 成長率을 보였고 1人當 國民所得은 10年間 536.2% 增加했다.<br/> 2. 1人 1日當 食品攝取量은 1970년부터 1979年까지 穀類가 7.5%, 豆類 34.5%, 감자류 46.0% 減少했고 新鮮野菜類 50%, 果實類 47.1%, 肉類 31.3%, 新鮮魚貝類 79.1%, 卵類 45.5%, 乳類 112.2%, 油脂類 21.2%가 增加했다.<br/> 3. 國民 成人 1人 1日當 平均 營養素의 攝取狀態는 熱量攝取量이 每年 한국인 영양권장량에 미달되었고, 蛋白質攝取量은 1970年에는 73.4g, 1979年에는 81.9g이었고 이 중 動物性蛋白質 攝取量도 권장량에 비해 부족되었고, 脂肪의 攝取量은 1970年에 21.8g, 1979年에는 33.2g으로 增加되었다.<br/> 4. 無質中 calcium과 鐵分의 攝取量은 每年 植物性食品에서 大部分 攝取하였으며 권장량에 비해 不足되었다.<br/> 5. Vitamin A의 攝取量도 大部分 植物性으로 攝取했고, thiamin, riboflarin, niacin, ascorbic acid 攝取量은 모두 調理時의 損失을 고려하면 권장량에 末達되었다.<br/> 이 中 특히 riboflavin은 권장량의 50% 정도밖에 안되었다.<br/> 以上에서와 같이 1970年에서 1979年까지 動物性食品의 攝取比率이 增加되고 植物性食品의 攝取比率이 減少되었는데도 大部分의 營養素攝取量이 권장량에 末達된 것은 아직도 植物性食品에 대한 攝取 依存度가 높기 때문이며 植物性食品의 攝取를 높일 수 있는 國民의 食生活이 改善策이 硏究되어야 겠다. Trend of foods and nutrients intake per person per day in Korea from 1970 to 1979, based on reports on national nutrition survey, was analyzed The average rates of population, GNP and per capita national income were 1.7, 9.6 and 536.2%, respectively‘ The consumption of cereals, pulses and potatoes was decreased; whereas that of fresh vegdtables, fruits, meat, fresh fish and shells, eggs, milks, and fats and oils was increased. Calorie intake was remained essentially constant, but protein and fat intake was increased by 11.6% and 52.3%, respectively, in ten years. The main sources for calcium, iron and vitamin A were vegetables. Among various nutrients, intake of riboflavin and animal protein were l ess than RDA.

청주지역 모유영양아의 수유기간별 모유 섭취량과 체중 변화에 관한 연구

한양일,설민영,김을상 서원대학교 응용과학연구소 1995 응용과학연구 Vol.4 No.1

The longitudial changes on human milk volume per day and lactational performance of 27 Korean lactating women from 0.5 to 6 months after parturition have been studied by test-weighing method in Cheongju area. The human milk volume per day increased to 2 months postpartum, and then decreased during lactation. The mean volume(g/day) at 0.5, 1, 2, 3, 4, 5 and 6 months were 634, 810, 847, 840, 844, 810 and 732 respectively. The average volume were 791(±149) and 786(±131)g for primiparae and multiparae, respectively. The overall mean volume was 788±135g/day. The mean volume between primiparae and multiparae during lactation was not significantly different. However, the volume at 0.5 months postpartum was significantly lower than other periods. The number of feeding per day was 10.1(±1.9) at 0.5 months postpartum, which was consitently decreased. However, the mean intake volume per feeding was increased from 53.5(±10.9)g at 0.5 month to 102.3(±20.2)g at 6 months postpartum. Infant milk intake per day tended to increase during lactation. The mean intakes at 0.5, 1, 2, 3, 4, 5 and 6 months were 532, 734, 756, 785, 767, and 710g respectively. The average intakes were 752 and 660g for boys and girls, respectively. The overall intake was 711g/day. The milk intake between boys and girls at 1, 2, 4 months and mean value was significantly different(p<0.05). The mean birth weight was 3.4±0.5kg, which increased significantly during lactation(p<0.001), but maternal weight remained fairly constant.

실험적으로 유발한 Vibrio vulnificus 감염에 관한 병리조직학적 연구

한을남,허충림,양문호 慶熙大學校 醫科大學 1987 慶熙 醫大 論文集 Vol.12 No.1

방사절점보간 무요소법을 이용한 응력집중 해석

한상을(Han Sang-Eul),양재근(Yang Jae-Guen),주정식(Joo Jung-Sik) 대한건축학회 2007 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.27 No.1

A Meshfree is a method used to establish algebraic equations of system for the whole problem domain without the use of a predefined mesh for the domain discretization. A point interpolation method is based on combining radial and polynomial basis functions. Radial basis function(RBF) augmented with polynomials is used to construct the shape functions. These shape functions possess delta function property that makes the numerical procedure very efficient and many techniques used in the finite element method can be adopted easily. Furthermore, the interpolation function passes through all scattered points in an influence domain and thus shape functions are of delta function property. This makes the implementation of essential boundary conditions much easier than the meshfree methods based on the moving least-squares approximation. A plate with a hole subjected to a tensile load and a V-notched tension specimen are analyzed and their stress distributions match well with those obtained by finite element commercial software ANSYS or the available literature.

청주지성 세시음식에 관한 연구

김을상,설민영,한양일 한국식생활문화학회 1991 韓國食生活文化學會誌 Vol.6 No.3

A study on the foods of annual custom in Cheongju area was done to know the present practices and compare with Dongkooksesiki (東國歲時記), Youlyangsesiki (洌陽歲時記), Kyoungdojabji (京都雜誌), and the results of a study on the gala foods in Kangweon province. Those annual custom which are celebrated in Cheongju area are Seolnal (New Year's Day) (100%), Chuseok (Harvest Moon Day) (100%), Daeboreum (the 15th of January) (92.6%), Dongji (the winter solstice) (75.2%), and Sambok (the period of summer heat) (67.4%) in the order of higher percentages. No subjects for this survey are keeping on celebrating Junghwa (servants day), Jungwon (the 15th day of the 7th lunar month), and Nabpyoung. Foods of annual custom on Chuseok and Seolnal had a greater variety, compared with those enjoyed on other annual custom. Foods of annual custom such as Ddugguk, Mandoo on Seolnal, Ogokbab, Mugeunnamul, and Buryum on Daeboreum, Songpyun on Chuseok, Patjuk on Dongji were being enjoyed by most people. But the other foods of annual custom are enjoyed in a lower percentage or almost forgotten.

제2족지 내족저부 유리피판을 이용한 수지첨부의 연부조직 결손 재건술

이두양,윤을식,한승규,이병일,구상환,박승하,김우경 大韓成形外科學會 2001 Archives of Plastic Surgery Vol.28 No.6

파킨슨병 모델 동물에서 GDNF 아데노바이러스 벡터를 이용한 유전자치료

성기한(Ki-Han Sung),윤인규(In-Gyu Yoon),정현실(Hyun-Sil Jung),김을선(Eul-Seon Kim),김현주(Hyun-Joo Kim),김양호(Yang-Ho Kim),윤 식(Sik Yoon),김봉선(Bong-Seon Kim),김재봉(Jae-Bong Kim),백선용(Sun-Yong Baek) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.6

6-OHDA를 이용하여 실험적으로 유발한 파킨슨병 모델 동물을 대상으로 유전자 치료 목적으로 GDNF 유전자를 포함 하는 아데노바이러스 벡터(Ad-GDNF)를 주사하여 행동 변화와 줄무늬체와 흑색질의 tyrosine hydroxylase (TH) 면역반응양성의 도파민성 세포의 형태학적 변화를 연구하였다. 성숙한 수컷 Sprague-Dawley계 흰쥐의 줄무늬체에 6-OHDA를 주사하고, 1주 후에 apomorphine에 의한 회전 운동 양상 을 측정하여 분당 7회 이상 회전하는 동물은 파킨슨병 모델 동물에 해당하는 것으로 하였다. 이들 동물의 줄무늬체에 Ad-GDNF를 주사하고 1주 후에 apomorphine에 의한 행동 양상을 다시 측정하고, 이어서 심장 관류고정을 실시하여 형태 학적 변화를 관찰하였다. Ad-GDNF 대신에 LacZ 유전자를 포함하는 아데노바이러스 벡터(Ad-LacZ)를 주사한 것을 대조 군으로 이용하였다. 1. 파킨슨병 모델 동물의 줄무늬체에 Ad-GDNF를 주사한 동물에서 apomorphine에 의한 회전 운동 수가 유의하게 감소 하였다. 그러나 Ad-LacZ를 주사한 대조군에서는 회전 운동 수의 변화를 관찰할 수 없었다. 2. 회전 운동 수가 감소한 동물의 줄무늬체에 존재하는 TH 면역반응 양성의 축삭 종말은 대조군의 75% 수준으로 Ad- LacZ를 주사한 37%에 비하여 현저히 증가하여 TH 면역반응 양성 축삭종말의 퇴행성 변화가 방지되었다. 3. 흑색질의 TH 면역반응 양성 신경세포의 수는 Ad-GDNF를 주사한 군에서는 대조군의 82% 수준으로 Ad-LacZ를 주사한 군의 60% 수준에 비하여 유의하게 증가되어 있어서 6-OHDA에 의한 신경세포의 사멸이 방지되는 것을 알 수 있었다. 이상의 결과로 미루어 파킨슨병 모델 흰쥐의 줄무늬체에 Ad-GDNF를 한차례 주사하여 1주 후에 줄무늬체와 흑색질의 도파민성 세포의 사멸이 방지되는 것을 확인하였다. A recombinant adenoviral vector encoding human GDNF gene (Ad-GDNF) was developed to investigate the effect of GDNF gene in 6-OHDA-induced Parkinson’s disease rat. The changes of rotatory behavior and density of THimmunoreactive axon terminals and number of cell bodies were observed by the GDNF expression. Adult male Sprague-Dawley rats were used. Parkinson’s disease (PD) rat models were prepared by the injection of 6-OHDA into the striatum. After 1 week, the animals showing apomorphine-induced rotatory behavior above 7 turns/ min were defined as PD rat model. Ad-GDNF was injected into the striatum of animal model and tested the apomorphine- induced rotatory behavior at 1 week after injection. These animals were perfused with Zamboni fixative to investigate the morphological changes after rotatory behavior test. Instead of Ad-GDNF, Ad-LacZ injected PD rats were used as a control. The following results are obtained: 1. The apomorphine-induced rotational behavior was significantly reduced by the treatment of PD rat by the injection of Ad-GDNF. The Ad-LacZ injected PD rat showed no change in rotatory behavior. 2. The density of TH-ir axon terminals in the striatum was significantly increased by the Ad-GDNF injection (75% of normal side), but there was no change in the density by the Ad-LacZ injection (37% of normal side) compared to 6-OHDA lesioned striatum. This means the Ad-GDNF injection prevented the degenerative change of TH-ir axon terminals in the stritum of the PD rat. 3. The number of TH-ir cell body was significantly recovered by the Ad-GDNF (82% of normal side), but there was not recovered by the Ad-LacZ injection (60% of normal side) compared to 6-OHDA lesion. This means the retrogradely transported Ad-GDNF induced the TH expression in the dopaminergic neurons of the substantia nigra. Gene therapy with Ad-GDNF prevented the degeneration of dopaminergic neurons and axon terminals in the 6- OHDA-induced PD rat.