Lactobacillus casei Zhang Prevents Jejunal Epithelial Damage to Early-Weaned Piglets Induced by Escherichia coli K88 via Regulation of Intestinal Mucosal Integrity, Tight Junction Proteins and Immune Factor Expression

( Yuying Wang ),( Xue Yan ),( Weiwei Zhang ),( Yuanyuan Liu ),( Deping Han ),( Kedao Teng ),( Yunfei Ma ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.6

Farm animals such as piglets are often affected by environmental stress, which can disturb the gut ecosystem. Antibiotics were commonly used to prevent diarrhea in weaned piglets, but this was banned by the European Union due to the development of antibiotic resistance. However, the use of probiotics instead of antibiotics may reduce the risk posed by pathogenic microorganisms and reduce the incidence of gastrointestinal diseases. Therefore, this study was conducted to investigate the effects of Lactobacillus casei Zhang on the mechanical barrier and immune function of early-weaned piglets infected using Escherichia coli K88 based on histomorphology and immunology. Fourteen-day-old weaned piglets were divided into a control group and experimental groups that were fed L. casei Zhang and infected with E. coli K88 with or without prefeeding and/or postfeeding of L. casei Zhang. The L. casei Zhang dose used was 107 CFU/g diet. Jejunum segments were obtained before histological, immunohistochemical, and western blot analyses were performed. In addition, the relative mRNA expression of toll receptors and cytokines was measured. Piglets fed L. casei Zhang showed significantly increased jejunum villus height, villus height-crypt depth ratio, muscle thickness, and expression of proliferating cell nuclear antigen and tight junction proteins ZO-1 and occludin. The use of L. casei Zhang effectively reduced intestinal inflammation after infection. We found that L. casei Zhang feeding prevented the jejunum damage induced by E. coli K88, suggesting that it may be a potential alternative to antibiotics for preventing diarrhea in early-weaned piglets.

회화적 관점에서 본 요양(遠陽)지역 후한(後漢) 묘실벽화(墓室壁晝)의 특징

장함 ( Zhang Han ) 단국사학회 2023 史學志 Vol.63 No.-

벽화는 미술의 한 분야로 제작 당시의 시대적 상황과 미술적 특징을 모두 반영하고 있다는 점에서 역사적으로 미술사적으로 매우 중요한 연구 가치를 지니고 있다. 요양(遠陽)지역의 경우 무덤에 벽화가 그려지기 시작한 것은 후한(後漢)대부터이며, 이후 위진(魏晉)시기까지 이어지고 있다. 그동안 요양지역 후한 묘실벽화 연구는 대부분 역사학과 고고학 분야에 집중되어 있었고, 미술사 혹은 미술학적 관점에서의 연구는 극소수였다. 이에 본 논문에서는 미술학적 연구 방법에 입각하여 벽화의 전체적인 소재, 조형, 색깔, 회화 기법 등을 분석하고, 그 특징을 찾아내어 무덤 벽화의 미술사적 의미를 찾아보고자 한다. 본 연구는 요양지역 후한 벽화무덤 중 비교적 벽화가 잘 남아있는 구성동문리벽화묘(舊域東門襄壁晝墓), 북원1호벽화묘(北園號壁晝墓), 봉자대1호벽화묘(棒子臺1號壁晝墓), 삼도호거기벽화묘(三道壞車騎壁晝墓)를 대상으로 하였다. 이들 벽화의 특징을 분석해 본 결과 한나라 묘실벽화의 제작 기법이 대부분 그대로 적용되고 있으나, 일부 색채와 구도, 회화기법 등에서 차이점이 존재하고 있음을 확인할 수 있었다. Mural painting is a field of art and has a very important research value in art history in that it reflects both the situation of the times at the time of production and the characteristics of art. In the case of the Liaoyang(遼陽) area, murals began to be painted on the tombs from the Later Han Dynasty(後漢) to the Weijin Dynasty(魏晉). Until now, most of the research on tomb murals after recuperation has been concentrated in the fields of history and archaeology, and very few studies have been done from an art history or art perspective. Therefore, this paper analyzes the overall material, formation, color, and painting techniques of murals based on art research methods, finds their characteristics, and tries to find the historical meaning of art in tomb murals. The study covered Jiuchengdongmenri Mural Tomb(舊城東門里壁畫墓), BeiyuanNo.1(北園1号壁書墓), BangzitaiNo1(棒子台一考壁書墓), and Sandaohucheqi Tomb(三道壕車騎壁畫墓) where murals remain relatively well among the Later Han Dynasty Mural Tombs in the nursing area. After analyzing the characteristics of these murals, it was confirmed that although Han's Dynasty tomb wall painting techniques were applied almost exactly as they were, there are differences in some colors, composition, and painting techniques.

Genetic Screening for Mutations in the Chip Gene in Intracranial Aneurysm Patients of Chinese Han Nationality

Su, Li,Zhang, Yuan,Zhang, Chun-Yang,Zhang, An-Long,Mei, Xiao-Long,Zhao, Zhi-Jun,Han, Jian-Guo,Zhao, Li-Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

We performed a case-control study to investigate whether SNPs of CHIP might affect the development of IA in Chinese Han nationality. We believe we are the first to have screened IA patients for mutations in the CHIP gene to determine the association with these variants. The study group comprised 224 Chinese Han nationality patients with at least one intracranial aneurysm and 238 unrelated healthy Han nationality controls. Genomic DNA was isolated from blood leukocytes. The entire coding regions of CHIP were genotyped by PCR amplification and DNA sequencing. Differences in genotype and allele frequencies between patients and controls were tested by the chi-square method. Genotype and allele frequencies of the SNP rs116166850 was demonstrated to be in Hardy-Weinberg equilibrium. No significant difference in genotype or allele frequencies between case and control groups was detected at the SNP. Our data do not support the hypothesis of a major role for the CHIP gene in IA development in the Chinese Han population.

Differences in Epidermal Growth Factor Receptor Gene Mutations and Relationship with Clinicopathological Features in NSCLC Between Uygur and Han Ethnic Groups

Zhang, Yan,Wang, Qiang,Han, Zhi-Gang,Shan, Li Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: To investigate differences in mutations of epidermal growth factor receptor (EGFR) gene and relationships with clinicopathological features in patients with non-small cell lung cancer (NSCLC) between Uygur and Han ethnic groups. Methods: The Scorpions amplification refractory mutation system (Scorpions ARMS) was used to measure mutations in exons 18, 19, 20 and 21 of the EGFR gene in paraffin-embedded tumor tissue from NSCLC cases, and statistical analysis was performed to investigate links with clinicopathological features in different histological types of NSCLC. Results: Results from ARMS testing showed EGFR mutations in tumor tissues from six (6) of 50 NSCLC patients of Uygur ethnic group, with a positive rate of 12.0%; four of them (4) had exon 19 deletion in EGFR, and two (2) had L858R point mutation in exon 21 of EGFR. Statistically significant difference was noted in EGFR genetic mutation between adenocarcinoma and non-adenocarcinoma (P < 0.05), but no differences with gender, age group, smoking status, or stage (P > 0.05). EGFR mutations were detected in tumor tissues from 27 of 49 NSCLC patients of Han ethnic group, with a positive rate of 55.1%; 19 of them had exon 19 deletions, seven (7) had L858R point mutations in exon 21 of EGFR and one (1) had mutations in both exon 18 G719X and exon 20 T790M of EGFR. Statistically significant differences were noted in EGFR genetic mutations between genders and between adenocarcinoma and non-adenocarcinoma (P<0.05), but not with age group, smoking status, or stage (P > 0.05). Conclusion: Statistically significant differences were noted in the positive rates of EGFR genetic mutations in NSCLC patients between Uygur and Han ethnic groups, with lower positive rates for the Uygur cases.

Enhancement of transglutaminase production in Streptomyces mobaraensis DSM 40587 by non-nutritional stress conditions: Effects of heat shock, alcohols, and salt treatments

Lili Zhang,Lanwei Zhang,Huaxi Yi,Ming Du,Yingchun Zhang,Xue Han,Zhen Feng,Jingyan Li,Yuehua Jiao,Yanhe Zhang,Chunfeng Guo 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.7

Stress-mediated bioprocess is a strategy designed to enhance biological target productivity. In this study,an attempt was made to enhance transglutaminase (TGase) production by Streptomyces mobaraensis by using different stress conditions including heat shock, alcohols and salt stress. Results showed that the effects of stress on TGase production depended on the type applied. For heat shock, TGase production (1.32 U/ml) was recorded maximum in the culture treated at 48 h post inoculation in water bath at 60 oC for 1 min. For alcohols treatment, the maximum activity of TGase (1.77 and 1.75 U/ml) was obtained when 3% methanol was added into the medium at 0 or 24 h of fermentation. However, a 3.5-fold increased production of TGase (3.8 U/ml) was observed in the medium supplemented with 0.2mol/l MgCl2 compared with the basic medium at the beginning of fermentation. In conclusion, TGase production from S. mobaraensis was improved by heat shock, methanol and salt stress treatments, MgCl2 stress was the most effective.

Magnesium isoglycyrrhizinate inhibits L-type Ca2+ channels, Ca2+ transients, and contractility but not hERG K+ channels

Yue Lin,Yuanyuan Zhang,Qiongtao Song,Tao Song,Xue Han,Ying Zhang,Xuan Zhang,Xi Chu,Fenghua Zhang,Li Chu,Jianping Zhang 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.10

To explore the cardiovascular protective effectsof Magnesium isoglycyrrhizinate (MI), especially theunderlying cellular mechanisms related to L-type calciumchannels and myocardial contractility, and to examine theeffects of MI on hERG K? current expressed in HEK293cells. We used the whole-cell patch clamp technique,video-based edge detection and dual excitation fluorescencephotomultiplier systems to explore the effect of MIon L-type Ca2? currents (ICa-L) and cell contraction in ratcardiomyocytes. We also examined the rapidly activatingdelayed rectifier potassium current (IKr) expressed inHEK293 cells using a perforated patch clamp. MI inhibitedICa-L in a dose-dependent manner, with a half-maximalinhibitory concentration (IC50) of 0.22 mg/ml, and themaximal inhibitory effect was 61.10 ± 0.59%. MI at aconcentration of 0.3 mg/ml reduced cell shortening by24.12 ± 3.97% and the peak value of the Ca2? transient by36.54 ± 4.96%. MI had no significant influence on hERGK? channels expressed in HEK293 cells at all testpotentials. MI exerts protective effects on the heart via theinhibition of ICa-L and cell shortening in rat cardiomyocytes. However, MI had no significant influence on IKr;thus, MI may exert cardioprotective effects without causingdrug-induced long QT syndrome.

Evaluation of Relative Bioavailability of 25-Hydroxycholecalciferol to Cholecalciferol for Broiler Chickens

Han, J.C.,Chen, G.H.,Wang, J.G.,Zhang, J.L.,Qu, H.X.,Zhang, C.M.,Yan, Y.F.,Cheng, Y.H. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.8

This study was conducted to evaluate the relative bioavailability (RBV) of 25-hydroxycholecalciferol (25-OH-$D_3$) to cholecalciferol (vitamin $D_3$) in 1- to 21-d-old broiler chickens fed with calcium (Ca)- and phosphorus (P)-deficient diets. On the day of hatch, 450 female Ross 308 broiler chickens were assigned to nine treatments, with five replicates of ten birds each. The basal diet contained 0.50% Ca and 0.25% non-phytate phosphorus (NPP) and was not supplemented with vitamin D. Vitamin $D_3$ was fed at 0, 2.5, 5.0, 10.0, and $20.0{\mu}g/kg$, and 25-OH-$D_3$ was fed at 1.25, 2.5, 5.0, and $10.0{\mu}g/kg$. The RBV of 25-OH-$D_3$ was determined using vitamin $D_3$ as the standard source by the slope ratio method. Vitamin $D_3$ and 25-OH-$D_3$ intake was used as the independent variable for regression analysis. The linear relationships between the level of vitamin $D_3$ or 25-OH-$D_3$ and body weight gain (BWG) and the weight, length, ash weight, and the percentage of ash, Ca, and P in femur, tibia, and metatarsus of broiler chickens were observed. Using BWG as the criterion, the RBV value of 25-OH-$D_3$ to vitamin $D_3$ was 1.85. Using the mineralization of the femur, tibia, and metatarsus as criteria, the RBV of 25-OH-$D_3$ to vitamin $D_3$ ranged from 1.82 to 2.45, 1.86 to 2.52, and 1.65 to 2.05, respectively. These data indicate that 25-OH-$D_3$ is approximately 2.03 times as active as vitamin $D_3$ in promoting growth performance and bone mineralization in broiler chicken diets.

Research and Design for AHP Module based on Android System

Zhang Hong-Han,Wang Rujun 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.1

An Ultra-narrow Bandwidth Filter for Daytime Wind Measurement of Direct Detection Rayleigh Lidar

Han, Fei,Liu, Hengjia,Sun, Dongsong,Han, Yuli,Zhou, Anran,Zhang, Nannan,Chu, Jiaqi,Zheng, Jun,Jiang, Shan,Wang, Yuanzu Optical Society of Korea 2020 Current Optics and Photonics Vol.4 No.1

A Rayleigh Lidar used for wind detection works by transmitting laser pulses to the atmosphere and receiving backscattering signals from molecules. Because of the weak backscattering signals, a lidar usually uses a high sensitivity photomultiplier as detector and photon counting technology for signal collection. The capturing of returned extremely weak backscattering signals requires the lidar to work on dark background with a long time accumulation to get high signal-to-noise ratio (SNR). Because of the strong solar background during the day, the SNR of lidar during daytime is much lower than that during nighttime, the altitude and accuracy of detection are also restricted greatly. Therefore this article describes an ultra-narrow bandwidth filter (UNBF) that has been developed on 354.7 nm wavelength of laser. The UNBF is used for suppressing the strong solar background that degrades the performance of Rayleigh wind lidar during daytime. The optical structure of UNBF consists of an interference filter (IF), a low resolution Fabry-Perot interferometer (FPI) and a high resolution FPI. The parameters of each optical component of the UNBF are presented in this article. The transmission curve of the aligned UNBF is measured with a tunable laser. Contrasting the result of with-UNBF and with-IF shows that the solar background received by a Licel transient recorder decreases by 50~100 times and that the SNR with-UNBF was improved by 3 times in the altitude range (35 km to 40 km) compared to with-IF at 10:26 to 10:38 on August 29, 2018. By the SNR comparison at four different times of one day, the ratio-values are larger than 1 over the altitude range (25~50 km) in general, the results illustrate that the SNR with-UNBF is better than that with-IF for Rayleigh Lidar during daytime and they demonstrate the effective improvements of solar background restriction of UNBF.

Effects of $1{\alpha}$-Hydroxycholecalciferol and Phytase on Growth Performance, Tibia Parameter and Meat Quality of 1- to 21-d-old Broilers

Han, J.C.,Yang, X.D.,Zhang, L.M.,Li, W.L.,Zhang, T.,Zhang, Z.Y.,Yao, J.H. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.6

This experiment was conducted to investigate the effects of interaction between $1{\alpha}$-hydroxycholecalciferol ($1{\alpha}$-OH $D_3$) and phytase on growth performance, parameters of tibia and plasma, and meat quality of 1- to 21-d-old broilers. Two hundred and forty male, 1-d-old Arbor Acres broilers were randomly assigned to 20 cages, with 12 chicks per cage. Five treatments were designed, with four cages each. A 2${\times}$2 factorial experiment was designed to test 0 and 5 ${\mu}g/kg$ of $1{\alpha}$-OH $D_3$ in combination with 0 and 500 U/kg of phytase. A basal diet was formulated to contain 2.9 g/kg of non-phytate phosphorus (NPP), and the control diet was formulated to contain a normal level of NPP (4.5 g/kg). Results showed that $1{\alpha}$-OH $D_3$ alone increased tibia ash, contents of calcium and phosphate, breaking strength, concentrations of plasma calcium and phosphate, and water-holding capacity of breast and thigh meat, while it decreased growth of broilers. Phytase alone improved performance and tibia quality. Although growth of broilers was lower than that of the positive control when the diet was supplemented with $1{\alpha}$-OH $D_3$ and phytase, tibia quality was significantly improved by the addition of $1{\alpha}$-OH $D_3$ and phytase. These data suggest that interaction between $1{\alpha}$-OH $D_3$ and phytase at 2.9 g/kg of dietary NPP could significantly increase bone quality of 1- to 21-d-old broilers, while not improving growth performance.