남한강 영월지역의 떼꾼에 관한 연구

이한길 ( Han Kil Lee ),이승철 ( Seung Cheol Lee ) 한국도서학회 2013 韓國島嶼硏究 Vol.25 No.3

The existence of raft carrier (dde-ggun) and the characteristics of their life style in Yeoungwol region, the south of Han River waterway, were studied. Until now, the different definitions of raft men were not unified. Thus terms related to raft men were arranged and unified. The raft men`s route of south Han River starts from Yeoungwol and Joungsun, passing through Danyang and Choongju, Yangpyoung, Yeoju, Paldang and duckso, finally arriving in Seoul. Thus the work and life style of raft men in the Yeoungwol region would contain characteristics of all the regions throughout the moving route. The felling operation ritual ceremony, waterway pattern, method of raft making and everything related to the water route of raft are likely to differ from the North. In this sense, our work has limitations in that it only discovers the characteristics involved in south Han River waterway. Thus if both the south and north Han River raft men are observed and analyzed, the complete story of raft men in Gangwon province will be known.

한국재래흑염소에서 발정 및 과배란 유도와 외래유전자 주입에 적합한 1세포기 수정란의 채취

신상태,이두환,김명철,이운규,이철상,한용만,이경광 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

Three different treatments for induction of estrus in Korean native black goats were compared: follicle stimulating hormone(FSH, FSH-p^TM), FSH combined with MAP(intravaginal impregnated sponges, Veramix^ⓡ, containing 60 ㎎ medroxy progesterone acetate for 14 days), and FSH combined with progesterone(Ovaron^ⓡ, 10 ㎎ IM for 10 days) and PGF_2α(Lutalyse^ⓡ, 3 ㎎ IM at first FSH injection). FSH for inducing estrus and superovulation was given a total 20 ㎎ intramuscularilly in decreasing dosage injections twice daily over 4 days. The MAPs were withdrawn at the 3rd day of FSH injection. Estrus observations were conducted every 6 hours from last FSH injection for 24 hours by placing the does with fertile male goats. Estrus and superovulation were more successfully induced with treatment of MAP + FSH than other treatments(FSH only, or progesterone + PGF_2α + FSH) (estrus induction; 100 vs 42.8 and 71.4%, ovulation points; 11.4 vs 5.4 and 4.4, respectively). The effect of gonadotropin releasing hormone(GnRH) on the ovulation rate was also examined. However, no difference was observed for inducing ovulation with treatment or dosage(100 ㎍ 200 ㎍) of GnRH. Low midline laparotomies were performed, and then ovarian responses (ovulations and follicular development) were examined by exteriorization of the reproductive tracts. Ova were recovered from oviducts by retrograde flushing 60-146 hours after MAP removal, and were classified the developmental stages. Overall 66.1% (236/357) of recovery rate was obtained from 30 superovulated does. The optimal recovery time of microinjectable 1-cell zygotes was approximately 72-76 hours after MAP removal.

크롬(Ⅵ)의 체내 흡수와 소실속도에 관한 연구

김현영,이성배,임철홍,이권섭,정용현,이종성,한정희,전윤석,황호순,이용묵 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.1

The CrO3 mostly used in plating. metal surface disposal, leather, cosmetic manufacturing, as an experiment material by repeatedly inhaling and exposure the male S.D. rats at a 0.00, 0.2., 0.50, 1.25 mg/㎡ concentration(particle size: 0.5-0.5 aerosol)6hours a day, 5day a week in 13weeks comparing with 2weeks, 8weeks of recovery group about the noxiousness of the experiment animal and the reduce scale of the CrO3 in the internal organ especially in blook and respiratory organ with the period of convalescent and clearance. The experiment results which we received are as follows. 1. In blood the RBC, HGB and HCT experiment, rats with 0.20, 0.50 mg/㎡ concentration showed that there were some decreases but not dependent. The kidneys absolute weight compared with control group was reduced intentionally(p〈0.05) and the lungs absolute weight compared with control group showed intentional increase(p〉0.05). 2. After the exposure of the experiment material, the whole blook, l\blood plasma and red blood cell in blood by (x): the period of convalescent, per (y); the decreasing of Cr concentration, was y=66.51 e -0.057x, y=67.2 e-0.101x, y=70.01 e-0.030 in 0.50 mg/㎡ exposure concentration by calculating the clearance coefficient of correlation, and the half life (day)was estimated 12.0, 6086, 23.0 each. 3, After the exposure of the experiment material, the experiment animals lung, liver and kidneys by(x); the period of convalescent, per (y); the decreasing of Cr conentration, was y=1808 e-0.00493x, y=12.02e-0.0297x, y=67.61 e-0.0292x in 0.50mg/㎡ exposure concentration by calculating the clearance coefficient of correlation, and the half life(day)was estimated 140.6, 23.3, 23.7, each, and including lung, liver with all of the experiment internal organs, the Cr clearance decreased as the exposure concentration increased.

SD Rats를 이용 1-hexene의 흡입독성 연구

김현영,임철홍,정용현,이권섭,이성배,이준연,한정희,전윤석,이용묵 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4

The purpose of this study was to investigate the acute (4 hours) and repeated-dose (6 hours a day, 5 days a week, 4 weeks) toxic effects of I -hexene on Sprague- Dawley (SD) rats which were treated by inhalation. The results were as follows; I. The median lethal concentration (LC_(50)) was estimated 52,694 ppm (confidence limit 95%; 49,494~55,447 ppm) in acute inhalation. Abnormal clinical signs related to the l-Hexene were not observed with the acute inhalation dose. Gross findings of necropsy revealed on evidence of specific toxicity related to the 1-hexene, II. By repeated inhalation exposure the body weight of male were more or less reduced by the dose of 2,500 ppm and 5,000 ppm compared with control group. However there were no significant variation hematology and blood biochemistry for the exposed rats compared with the control rats. Abnormal clinical signs and gross findings of necropsy related to the 1-hexene were not shown. In conclusion when we exposed I-hexene to SD rats for 4 weeks, 5 days per week, 6 hours per day, the Lowest observed effect level (LOEL) was over 2,500 ppm and Non observed effect level (NOEL) was below 500 ppm.

불안정한 바닥위에서 발목각도가 기립균형에 미치는 영향

이한숙,권혁철 한국전문물리치료학회 1997 한국전문물리치료학회지 Vol.4 No.3

양측 양성성대질환에 대한 연구

안철민,정덕희,한규철,박상준,이건영 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.7

관상동맥 시술 도중 발생한 좌주간부 관상동맥 박리에 대한 스텐트 삽입 치료의 단장기 임상 결과 : acute and long-term results

이세환,이승환,홍명기,김영학,이철환,한기훈,송종민,강덕현,송재관,김재중,박성욱,박승정 대한내과학회 2004 대한내과학회지 Vol.66 No.6

목적 : 심도자 사용 시술과 관련된 좌주간부 관상동맥박리에 대한 적절한 치료는 아직 확실히 정립되지 않은 상태이다. 이에 본 연구는 좌주간부 관상동맥 박리에 대한 스텐트 삽입술의 단장기 임상결과를 후향적으로 분석하였다. 방법 : 좌주간부 관상동맥 박리에 대한 치료로 즉각적인 스텐트 삽입술을 시행하는 것이 안전하고 효과적인 방법임을 가설하였고, 이러한 즉각적인 스텐트 삽입을 시행한 10명의 환자를 대상으로 단장기적 임상경과를 의무기록 고찰과 전화 면담을 통해서 평가하였다. 결과 : 전체 환자 모두 처음부터 좌주간부 관상동맥에 유의한 협착을 가진 환자는 없었으며, 8명의 환자는 심도자의 조작으로 인한 박리가 발생한 경우였고(진단적 혈관 조영술 중에 3명, 유도 도자 조작 중에 5명), 나머지 2명은 다른 병변에 스텐트 삽입시술을 하는 중에 박리가 발생하였다. 이 10명의 환자에서 모두 즉각적인 스텐트 삽입술이 시행되었고, 4명의 환자에서 시술 도중 혈압 저하로 Intra-aortic Ballon Pump (IABP)를 장착하였다. 모든 환자에서 성공적으로 스텐트가 삽입되었고, 병원내 사망은 1명도 없었다. 6개월 추적 관상동맥 조영술은 8명의 환자에서 시행되었다. 혈관 조영상의 재협착(직경 협착 50% 이상)은 8명 모두에게서 관찰되지 않았으며, 퇴원 후 평균 31±25개월의 추적관찰 결과 주요 심장사건은 1건도 발생하지 않았다. 결론 : 비록 대상 환자가 적은 연구였지만 좌주간부 관상동맥 박리에 대한 스텐트 삽입술은 기술적으로 안전하고 빠르게 시행 할 수 있으며, 훌륭한 단장기적인 치료 효과를 보여준다. Background : The optimal treatment of patients with left main coronary artery (LMCA) dissection during catheter-based procedure remains uncertain. We retrospectively analyzed the acute and long-term results of bail-out stenting for LMCA dissection. Methods : In cases with significant LMCA dissection occurring during catheter-based procedure, prompt stent implantation may be safe and associated with favorable clinical outcome. We evaluated the acute and long-term results of bail-out stenting for LMCA dissection occurring during catheter-bases procedure in 1- patients. Results : Initially, there was significant stenosis of LMCA segment in these patients. Catheter-induced dissection occurred in 8 patients (during diagnostic angiography in 3 patients and guiding catheter manipulation in 5 patients). Two patients suffered dissection in the setting of stent deployment in other vessels. Therefore, bail-out stenting LMCA dissection was performed in a total of 10 patients. In 4 patients, hypotension developed and intra-aortic balloon pump was placed during procedure. Stents were successfully deployed in all patients. There was no in-hospital mortality. Six-month angiographic follow-up was performed in 8 patients. Angiographic restenosis(diameter stenosis 50%) was not observed in all patients at follow-up study. During a mean follow-up of 31±25 months after hospital discharge, there was no major adverse cardiac event (death, myocardial infarction, and target lesion revascularization). Conclusion : Bail-out LMCA stenting is technically feasible, and showed good acute and long-term results in a small series of patients.

Salpiglossis sinuata L.의 原形質體 分離 및 培養

李澈熙,韓那榮,白基燁 충북대학교 농업과학기술연구소 1993 農業科學硏究 Vol.10 No.2

In order to attempt a realistic somatic hybridization using S, sinuata, it is imperative that large numbers of viable protoplasts were routinely available. In addition, cultural conditions have to be fully optimized for plant regeneration in order to maximize the probability of recovery, not only of hybrid callus, but of hybrid plants. Therefore, this study was performed to establish the ideal method for this field and results were as followed : Protoplasts were isolated, where possible, from both greenhouse-grown leaf material and cultured cell sources (callus, cell suspension) of wild-type and cytoplasmic albino mutant. The optimum methods for protoplast isolation were successfully established. Generally, cell suspension was the ideal material for protoplast isolation. The method and medium for protoplast culture were also fully optimized with an analysis of their response to a range of protoplast culture media, culture methods (Liquid layer or liquid over agar culture) and initial plating densities. KP8 liquid medium, together with initial plating density of 2.5×104/ml, was most efficient for protoplast culture. The regeneration sequence of protoplast isolation through to whole plants was attempted. Rep-roducible organogenesis was induced by transferring p-calli (5-10mm in diam.) to MSP1, MSD3, MSD4 and MSZ media. The rooting of regenerated wild-type S. sinuata shoots was difficult, but it was still possible to establish plantlets in the greenhouse. Protoplast-derived shoot regenerants of the albino form failed to root.

Isolation and Culture of protoplasts in Chrysanthemum morifolium

李澈熙,韓那榮 충북대학교 농업과학기술연구소 1994 農業科學硏究 Vol.11 No.2

器內培養한 植物體의 잎이나 花片으로 부터 상당히 높은 數量의 活力을 가진 原形質體를 成功的으로 分離하였으나, 溫室에서 栽培한 植物體의 葉肉組織으로 부터 分離한 原形質體들은 종종 活力이 喪失되거나 汚染되는 傾向을 보였다. 原形質體 培養時 溫室에서 栽培한 植物體의 잎이나 花片으로 부터 分離한 原形質體들은 分裂하지 않았다. 組織培養한 植物體 葉肉細胞 유래의 原形質體들은 液體培養時 3-4일경 부터 分裂하기 시작하였으나 褐變되어 培養 10일경 죽었으며, agarose를 利用하여 굳힌 固體培地 위에 液體培養한 경우에도 상당히 높은 分裂頻度를 나타내었으나 약 20細胞期에 分裂이 中止되었다. Agarose를 利用한 固體培地와 混合하여 培養한 경우 細胞分裂이 가장 旺盛히 일어났으며, 培養後 약 30-35일경 콜로니를 形成하였다. 그러나 原形質體 由來의 캘러스로 부터 植物體의 再生은 이루어지지 않았다.

표준치료에도 불구하고 3차례나 반복해서 발생한 삼일열 말라리아

이해영,한주용,이기덕,박완범,김성한,강철인,문정주,오명돈,최강원 대한감염학회 2002 감염 Vol.34 No.6