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Wu, Bing-Li,Zou, Hai-Ying,Lv, Guo-Qing,Du, Ze-Peng,Wu, Jian-Yi,Zhang, Pi-Xian,Xu, Li-Yan,Li, En-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5
Lysyl oxidase-like 2 (LOXL2), a member of the lysyl oxidase (LOX) family, is a copper-dependent enzyme that catalyzes oxidative deamination of lysine residues on protein substrates. LOXL2 was found to be overexpressed in esophageal squamous cell carcinoma (ESCC) in our previous research. We later identified a LOXL2 splicing variant LOXL2-delta72 and we overexpressed LOXL2-delta72 and its wild type counterpart in ESCC cells following microarray analyses. First, the differentially expressed genes (DEGs) of LOXL2 and LOXL2-delta72 compared to empty plasmid were applied to generate protein-protein interaction (PPI) sub-networks. Comparison of these two sub-networks showed hundreds of different proteins. To reveal the potential specific roles of LOXL2- delta72 compared to its wild type, the DEGs of LOXL2-delta72 vs LOXL2 were also applied to construct a PPI sub-network which was annotated by Gene Ontology. The functional annotation map indicated the third PPI sub-network involved hundreds of GO terms, such as "cell cycle arrest", "G1/S transition of mitotic cell cycle", "interphase", "cell-matrix adhesion" and "cell-substrate adhesion", as well as significant "immunity" related terms, such as "innate immune response", "regulation of defense response" and "Toll signaling pathway". These results provide important clues for experimental identification of the specific biological roles and molecular mechanisms of LOXL2-delta72. This study also provided a work flow to test the different roles of a splicing variant with high-throughput data.
Hai-Nan Lan,Hai-Long Jiang,Wei Li,Tian-Cheng Wu,Pan Hong,Yu Meng Li,Hui Zhang,Huan-Zhong Cui,Xin Zheng 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.4
B-32 is one of a panel of monoclonal anti-idiotypic antibodies to growth hormone (GH) that we developed. To characterize and identify its potential role as a novel growth hormone receptor (GHR) agonist, we determined that B-32 behaved as a typical Ab2β based on a series of enzyme-linked immunosorbent assay assays. The results of fluorescence-activated cell sorting, indirect immunofluorescence and competitive receptor binding assays demonstrated that B-32 specifically binds to the GHR expressed on target cells. Next, we examined the resulting signal transduction pathways triggered by this antibody in primary porcine hepatocytes. We found that B-32 can activate the GHR and Janus kinase (2)/signal transducers and activators of transcription (JAK2/STAT5) signalling pathways. The phosphorylation kinetics of JAK2/STAT5 induced by either GH or B-32 were analysed in dose-response and time course experiments. In addition, B32 could also stimulate porcine hepatocytes to secrete insulin-like growth factors-1. Our work indicates that a monoclonal anti-idiotypic antibody to GH (B-32) can serve as a GHR agonist or GH mimic and has application potential in domestic animal (pig) production.
Wu, Fei,Zhao, Zuo-Hui,Ding, Sen-Tai,Wu, Hai-Hu,Lu, Jia-Ju Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10
Background: The high mobility group box 1 (HMGB1) protein is a widespread nuclear protein present in most cell types. It typically locates in the nucleus and functions as a nuclear cofactor in transcription regulation. However, HMGB1 can also localize in the cytoplasm and be released into extracellular matrix, where it plays critical roles in carcinogenesis and inflammation. However, it remains elusive whether HMGB1 is relocated to cytoplasm in clear cell renal cell carcinoma (ccRCC). Methods: Nuclear and cytoplasmic proteins were extracted by different protocols from 20 ccRCC samples and corresponding adjacent renal tissues. Western blotting and immunohistochemistry were used to identify the expression of HMGB1 in ccRCC. To elucidate the potential mechanism of HMGB1 cytoplasmic translocation, HMGB1 proteins were enriched by immunoprecipitation and analyzed by mass spectrometry (MS). Results: The HMGB1 protein was overexpressed and partially localized in cytoplasm in ccRCC samples (12/20, 60%, p<0.05). Immunohistochemistry results indicated that ccRCC of high nuclear grade possess more HMGB1 relocation than those with low grade (p<0.05). Methylation of HMGB1 at lysine 112 in ccRCC was detected by MS. Bioinformatics analysis showed that post-translational modification might affect the binding ability to DNA and mediate its translocation. Conclusion: Relocation of HMGB1 to cytoplasm was confirmed in ccRCC. Methylation of HMGB1 at lysine 112 might the redistribution of this cofactor protein.
Promoted Growth of Maize by the Phosphate Solubilizing Bacteria Isolated from North-east China
Wu, Hai-Yan,Wang, Li-Chun,Gao, Xing-Ai,Jin, Rong-De,Fan, Zuo-Wei,Kim, Kil-Yong,Zhao, Lan-Po Korean Society of Soil Science and Fertilizer 2011 한국토양비료학회지 Vol.44 No.1
A strain of phosphate solubilizing bacterium was isolated from rhizosphere and identified as Burkholderia sp. by 16S-rRNA gene sequence analyses. The bacterium was found to release gluconic acid and the solubilization of hydroxyapatite in the liquid medium by a significant drop in pH to 3.7 from an initial pH 7.0. The soluble-P concentration continuously increased during the incubation periods and the total amount of soluble P released in culture filtrate was detected at 990 mg $L^{-1}$ after 10 days of inoculation. Most promoted maize growth was found in the standard NPK (240-120-120 kg $ha^{-1}$) soil inoculation with Burkholderia sp. (Twenty milliliters/plant, 106 CFU) and also in the absence of Burkholderia sp. inoculation, the soil amended with only 2/3 levels of P gave significant higher plant yield compared to 1/3 levels of P or without P supplementation.
Hai Ming Huang,Yan Liu,Denghao Wu,Yue Zhong Wu,Tian Xin Wu 한국유체기계학회 2023 International journal of fluid machinery and syste Vol.16 No.1
Cavitation in the pump induces vibration and noise, which leads to the degradation of pump performance and damage to the impeller. Therefore, it is of great significance to accurately identify the cavitation state of the centrifugal pump. To determine the cavitation state using the vibration characteristic frequency of the centrifugal pump, it is necessary to accurately identify the characteristic frequency due to the noise's effect. Therefore, a cavitation fault diagnosis method of centrifugal pump based on a cohesive hierarchy algorithm was proposed. Firstly, Singular Value Decomposition (SVD) is used to de-noise the vibration signal. Then the root means square of the vibration signal after de-noising is obtained as the eigenvalue of the vibration signal. Secondly, the hierarchical clustering algorithm is used to classify the vibration eigenvalues and accurately identify the non-cavitation and cavitation states of centrifugal pumps. Finally, through experimental tests, the method can effectively and quickly identify the cavitation state of the centrifugal pump, with an accuracy of 95%. The study provides a new way for rapidly diagnosing centrifugal pump cavitation
( Hai Xiao Wang ),( Kuang Jie Wu ),( Yuan Sun ),( Yan Dong Li ),( Ming Yu Wu ),( Qian Qiao ),( Yuan Jiang Wei ),( Ze Guang Han ),( Bing Cai ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.11
The human glycoprotein, stanniocalcin 2 (STC2) plays multiple roles in several tumor types, however, its function and clinical significance in hepatocellular carcinoma (HCC) remain unclear. In this study, we detected STC2 expression by quantitative real-time PCR and found STC2 was upregulated in HCC tissues, correla ed with tumor size and multiplicity of HCC. Ectopic expression of STC2 markedly promoted HCC cell proliferation and colony formation, while silencing of endogenous STC2 resulted in a reduced cell growth by cell cycle delay in G0/G1 phase. Western blot analysis demonstrated that STC2 could regulate the expression of cyclin D1 and activate extracellular signal-regulated kinase 1/2 (ERK1/2) in a dominant-positive manner. Transwell chamber assay also indicated altered patterns of STC2 expression had an important effect on cell migration. Our findings suggest that STC2 functions as a potential oncoprotein in the development and progression of HCC as well as a promising molecular target for HCC therapy.
Promoted Growth of Maize by the Phosphate Solubilizing Bacteria Isolated from North-east China
Hai-Yan Wu,Li-Chun Wang,Xing-Ai Gao,Rong-De Jin,Zuo-Wei Fan,Kil-Yong Kim,Lan-Po Zhao 한국토양비료학회 2011 한국토양비료학회지 Vol.44 No.1
A strain of phosphate solubilizing bacterium was isolated from rhizosphere and identified as Burkholderia sp. by 16S-rRNA gene sequence analyses. The bacterium was found to release gluconic acid and the solubilization of hydroxyapatite in the liquid medium by a significant drop in pH to 3.7 from an initial pH 7.0. The soluble-P concentration continuously increased during the incubation periods and the total amount of soluble P released in culture filtrate was detected at 990 mg L<SUP>-1</SUP> after 10 days of inoculation. Most promoted maize growth was found in the standard NPK (240-120-120 kg ha<SUP>-1</SUP>) soil inoculation with Burkholderia sp. (Twenty milliliters/ plant, 106 CFU) and also in the absence of Burkholderia sp. inoculation, the soil amended with only 2/3 levels of P gave significant higher plant yield compared to 1/3 levels of P or without P supplementation.
First Report of an Unrecorded Nematode-Trapping Fungus Species Monacrosporium phymatopagum in Korea
Wu, Hai-Yan,Kim, Dong-Geun The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.3
A fungus that can capture nematodes by means of sessile adhesive knobs was isolated from rotten wood in Uiseong, Korea. It was found to produce single, spindle-shaped, 3-4 septate (commonly 4-septate) conidia, $44.8\;{\mu}m$ (range, $41.6-50.1\;{\mu}m$) long and $13.3\;{\mu}m$ (range, $10.7-15.4\;{\mu}m$) wide. Conidiophores were found to be hyaline, erect, straight, and $202.7-245.7\;{\mu}m$ high. On the basis of these morphological features, the fungus was identified as Monacrosporium phymatopagum. This is the first report of M. phymatopagum in Korea which can be a potential biological control resource of plant parasitic nematode.