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( Hela Ben Amor Ben Ayed ),( Behnam Taidi ),( Habib Ayadi ),( Dominique Pareau ),( Moncef Stambouli ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.3
The accumulation (internal and superficial distribution) of magnesium ions (Mg(2+)) by the green freshwater microalga Chlorella vulgaris (C. vulgaris) was investigated under autotrophic culture in a stirred photobioreactor. The concentrations of the three forms of Mg(2+) (dissolved, extracellular, and intracellular) were determined with atomic absorption spectroscopy during the course of C. vulgaris growth. The proportions of adsorbed (extracellular) and absorbed (intracellular) Mg(2+) were quantified. The concentration of the most important pigment in algal cells, chlorophyll a, increased over time in proportion to the increase in the biomass concentration, indicating a constant chlorophyll/biomass ratio during the linear growth phase. The mean-average rate of Mg(2+) uptake by C. vulgaris grown in a culture medium starting with 16 mg/l of Mg(2+) concentration was measured. A clear relationship between the biomass concentration and the proportion of the Mg(2+) removal from the medium was observed. Of the total Mg(2+) present in the culture medium, 18% was adsorbed on the cell wall and 51% was absorbed by the biomass by the end of the experiment (765 h). Overall, 69% of the initial Mg(2+) were found to be removed from the medium. This study supported the kinetic model based on a reversible first-order reaction for Mg(2+) bioaccumulation in C. vulgaris, which was consistent with the experimental data.
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Khaled Bellassoued,Fatma Ben Mansour,Habib Ayadi,Jos van Pelt,Abdelfattah Elfeki 한국식품영양과학회 2024 Journal of medicinal food Vol.27 No.1
Basil (Ocimum basilicum L.) is distributed worldwide and used in the food, pharmaceutical, and cosmeticindustries. Most applications are for the herb basil, recently the basil seeds have also been used commercially; however, littleis known about the nutritional and functional properties of the seeds. The present study aimed to investigate a possibleprotective effect of the methanol extract of O. basilicum seeds (MEOB), based on its phytochemical content, against kidneytoxicity induced by CCl4 in adult rats. A single dose of CCl4 was used to induce oxidative stress in rats, which wasdemonstrated by a significant rise of serum enzyme markers. MEOB was administrated for 15 consecutive days (200 mg/kgbody weight) to Wistar rats before CCl4 treatment and the effects on serum urea, creatinine, and uric acid, as well as thekidney superoxide dismutase, catalase, glutathione peroxidase, and glutathione activity and thiobarbituric acid reactivesubstances and protein carbonyl (PCO) levels were evaluated. In addition, histopathological examinations of kidneys wereperformed. In the positive control group, CCl4 induced an increase in serum biochemical parameters and triggered oxidativestress in the kidney. MEOB (200 mg/kg BW) resulted in significant reduction of CCl4-elevated levels of kidney markers, ureaand creatinine, and a significant increase of uric acid compared with the CCl4-only group. In addition, MEOB pretreatmentresulted in a significant reduction in lipid peroxidation and PCO levels in renal tissue compared with CCl4-exposed group. MEOB definitely could prevent the development of pathological changes in the kidneys. Overall, we conclude that MEOB iseffective in protecting renal function from CCl4 toxicity.
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