紅景天(Rhodiola sachlinensis)에서 항균성 물질의 분리 및 동정

심창주,이규희,정재홍,이상덕,김영호,오만진 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2004 한국식품저장유통학회지 Vol.11 No.1

홍경천의 천연 보존료로서의이용성을 검토하기 위하여 각종 용매로 홍경천 추출물을 제조하고 극성에 따라 순차분획, silica-gel chromatography, thin-layer chromatography, high perfomance liquid chromatography의 분리과정을 거쳐 순수 분리된 각 물질을 GC/MS(EI) spectrum, ¹H-NMR spectrum, ¹³C-NMR spectrum을 이용하여 항균물질을 동정하여 다음과 같은 결과를 얻었다. 홍경천에 대해 acetone, ethyl acetate, ethanol, methanol, chloroform, water의 각 용매로 추출한 결과 항균성분의 추출 용매로는 methanol이 가장 우수하였다. 홍경천 methanol 추출물을 n-hexane, chloroform, ethyl ether, ethyl acetate, n-butyl alcohol로 분획한 결과 ethyl acetate와 n-butyl alcohol 분획에서 우수한 항균력을 나타내었다. 홍경천 methanol 추출물은 그람양성 4균주와 그람음성 4균주에 대해 고체 배지의 경우 대체로 500 μg/mL에서 최소저해를 나타냈으며, 액체배지의 경우 100 μg/mL 내외에서 최소저해를 나타내었다.] Silica gel column chromatography에서 용매의 배율을 높여가며 용출한 결과 chloroform : methanol(8:2, v/v)의 비율에서 항균력이 가장 높게 나타났다. 분리된 항균성분을 silica-gel TLC plate에 용매의 비율을 높여가며 전개시켜 chloroform : methanol(9:1, v/v)에서 9개의 물질군을 얻을 수 있었으며, 그 중 항균력이 확인된 3개의 물질군을 HPLC와 NMR spectrum을 분석한 결과 gallic acid, (-)-epicatechin 및 kaempferol로 동정되었다. gallic acid, (-)-epicatechin 및 kaempferol의 항균물질 각각의 최소저해농도는 Staphylococcus aureus와 Listeria monocytogenes에 대해 gallic acid와 kaempferol 이 500 μg/mL, (-)-epicatechin이 100 μg/mL에서 저해를 나타내었다. The antimicrobial substances from Rhodiola sachalinensis were extracted, isolated and identified. The highest level of antimicrobial activity and its yield were obtained in methanol extract. The minimum inhibition concentrations of the methanol extract were 500 μg/mL on agar plate and 100 μg/mL in broth media for four gram positive and four gram negative microbials. The methanol extract was fractionated by n-hexane, chloroform, ethyl ether, ethyl acetate, and butanol, orderly. The separate was developed on the TLC plate with different solvent system ratio of chloroform and methanol. Nine substances were isolated from chloroform and methanol mixture(9:1, v/v). Among them, three isolates showed antimicrobial activity. Three substances separated by HPLC were identified by GC/MS(EI) spectrum and ^(1)H, ^(13)C-NMR spcetrum. They were gallic acid, (-)-epicatechin and kaempferol. The antimicrobial activites of each substances were shown gallic acid, (-)-epicatechin, kaempferol, orderly.

인삼첨가 Long Life면의 조직감과 관능적 특성

정재홍,심창주,신영,권효진,이규희,오만진 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.5

인삼이 밀가루의 아밀로그래프에 의한 호화 성질과 파리노그래프에 의한 반죽 성질에 미치는 영향을 조사하였다. 인삼첨가 LL면의 조직감과 관능적 특성에 미치는 영향을 분석하기 위하여 밀가루에 대하여 인삼을 5.0∼10% 첨가하여 LL면을 제조한 뒤 면의 조직 특성 분석 및 조리시험을 하고 관능검사를 하였다. 인삼을 첨가하면 아밀로그래프의 호화개시 온도를 3.1℃ 지연시켰으며, 최고 점도를 40B.U 감소시켰다. 파리노그래프의 흡수율은 1.2% 증가하였으며, 반죽의 안정도는 인삼의 첨가로 2.0분 길게 측정되었다. Hunter L값은 인삼 첨가량이 증가할수록 낮아졌으며, Hunter a, b값은 반대로 측정되었다. 인삼첨가 LL면의 층밀림 압출력은 첨가량의 증가에 따라 높아져 10.0%의 첨가는 대조구 18.65(㎏f)보다 2.3(㎏f) 높은 23.95(㎏f)로 측정되었으며, 경도도 대조구보다 5.4(㎏f) 높게 측정되었다. 인삼첨가 LL면의 무게 증가는 인삼의 첨가량이 증가할 때 다소 높게 나타났으나. 부피 증가는 오히려 높게 나타나 조직이 다소 치밀한 것으로 나타났다 용출량은 인삼량의 증가에 따라 약간 감소하였다. 미생물은 인삼의 첨가량이 증가할수록 줄어들어서 장기 저장이 가능하였다. 관능 검사 결과는 인삼첨가 LL면이 4.2 및 4.0으로 비교적 좋은 점수를 얻었다. 이같이 LL면 제조시 인삼 첨가량은 7.0%의 수준이 효과가 컸다. The influence of ginseng on the paste or gelatinization properties by amylograph and mixing properties by farinograph of wheat flour, and on quality properties, color, cooking quality, textural and sensory properties, and reducing microbial population of LL (Long Life) noodles was studied. The contents of ginseng used were from 5% to 10% based on flour weight. The viscosity property of wheat flour with ginseng was increased the initial pasting temperature but the amylograph peak viscosity were decreased in vice versa. The farinograph absorption, stability and breakdown were increased by ginseng. The whiteness of LL noodles manufactured with ginseng was lower than that of control. The shear extrusion force and hardness of LL noodles manufactured with ginseng were shown much higher value than those of control. At cooking quality examination of LL noodles manufactured with ginseng, weight of cooked LL noodles was decreased but volume was appeared in vice versa. Extraction amounts of LL noodles manufactured with ginseng during cooking were much smaller than those of control. Total counts of microorganism of LL noodles manufactured with ginseng were decreased during storage at 30℃. Sensory properties of cooked LL noodles which was manufactured with ginseng showed quite acceptable. Based on the cooking and sensory evaluation test, addition of 7.0% ginseng to wheat flour may be suitable for processing LL noodles.

Update of minimally invasive surfactant therapy

Shim, Gyu-Hong The Korean Pediatric Society 2017 Clinical and Experimental Pediatrics (CEP) Vol.60 No.9

To date, preterm infants with respiratory distress syndrome (RDS) after birth have been managed with a combination of endotracheal intubation, surfactant instillation, and mechanical ventilation. It is now recognized that noninvasive ventilation (NIV) such as nasal continuous positive airway pressure (CPAP) in preterm infants is a reasonable alternative to elective intubation after birth. Recently, a meta-analysis of large controlled trials comparing conventional methods and nasal CPAP suggested that CPAP decreased the risk of the combined outcome of bronchopulmonary dysplasia or death. Since then, the use of NIV as primary therapy for preterm infants has increased, but when and how to give exogenous surfactant remains unclear. Overcoming this problem, minimally invasive surfactant therapy (MIST) allows spontaneously breathing neonates to remain on CPAP in the first week after birth. MIST has included administration of exogenous surfactant by intrapharyngeal instillation, nebulization, a laryngeal mask, and a thin catheter. In recent clinical trials, surfactant delivery via a thin catheter was found to reduce the need for subsequent endotracheal intubation and mechanical ventilation, and improves short-term respiratory outcomes. There is also growing evidence for MIST as an alternative to the INSURE (intubation-surfactant-extubation) procedure in spontaneously breathing preterm infants with RDS. In conclusion, MIST is gentle, safe, feasible, and effective in preterm infants, and is widely used for surfactant administration with noninvasive respiratory support by neonatologists. However, further studies are needed to resolve uncertainties in the MIST method, including infant selection, optimal surfactant dosage and administration method, and need for sedation.

Treatment of congenital cytomegalovirus infection

Gyu Hong Shim 대한소아청소년과학회 2023 Clinical and Experimental Pediatrics (CEP) Vol.66 No.9

Congenital cytomegalovirus (CMV) is the most common cause of congenital infection worldwide, the most common nongenetic cause of sensorineural hearing loss in children, and a cause of neurodevelopmental disorders in the brain. Infants with symptomatic congenital CMV infection may benefit from hearing and neurodevelopmental outcomes, particularly if antiviral treatment is initiated within the first month of life. Infants with life-threatening symptoms are recommended to receive 2–6 weeks of intravenous ganciclovir and then switch to oral valganciclovir, and those without life-threatening symptoms are recommended to use oral valganciclovir during the entire 6-month period. During antiviral drug treatment, absolute neutrophil count, platelet count, blood urea nitrogen, creatinine, and liver function tests were performed to identify neutropenia, thrombocytopenia, renal failure, and liver failure. This review investigated the evidence to date of treating congenital CMV infection.

Which factors predict outcomes of neonates with hypoxic-ischemic encephalopathy following therapeutic hypothermia?

Shim, Gyu Hong The Korean Pediatric Society 2021 Clinical and Experimental Pediatrics (CEP) Vol.64 No.4

최소 침습 폐표면활성제 투여법

심규홍 ( Gyu Hong Shim ) 대한주산의학회 2015 Perinatology Vol.26 No.4

과거에는 호흡곤란증후군이 있는 미숙아는 기관 삽관과 함께 폐표면활성제를 투여하는 치료 주로 시행하였다. 그러나 최근에는 비강 내 양압환기요법과 같은 비침습적 환기를 적용하는 것이 출생 후 미숙아에서 조기에 기관 삽관을 하여 치료하는 것을 대체할 수 있는 합리적인 대안으로 생각된다. 최근에는 비강 내 양압환기요법이 기관삽관 후 침습적인 환기를 적용 받는 미숙아에 비하여 기관지폐 형성 이상 또는 사망의 위험도를 어느 정도 감소시키는 결과를 보여주었다. 최근에 미숙아의 호흡곤란에 일차 치료로 비침습적 환기를 적용하는 것이 급증하고 있는데 이에 반해 폐표면활성제를 언제 어떻게 투여하는지에 대해서는 여전히 어려운 문제로 남아있다. 이러한 문제점을 해결하기 위하여 출생 후 비강 내 양압환기요법을 받고 있는 자발호흡이 있는 미숙아에서 최소 침습 폐표면활성제 투여법이 고안되었다. 최소 침습 폐표면활성제 투여법에는 기도 내 도관 삽입법, 분무주입법, 후두 마스크법 및 인후 내 점적법 등이 있다. 최근 여러 임상연구에서는 기도 내 도관 삽입법을 이용한 폐표면활성제 투여법이 기관 삽관과 침습적 기계환기 적용 빈도를 줄여 단기적인 예후를 향상시키는 것을 보여주었다. 결론적으로 최소 침습 폐표면활성제 투여법은 비교적 안전하고, 실현가능하며, 효과적인 치료법으로 앞으로 국내에서도 미숙아의 호흡곤란증후군 치료에 많이 적용되리라 생각된다. For many years preterm infants with respiratory distress syndrome have been managed with a combination of intubation and surfactant replacement therapy. It is now recognized that applying noninvasive ventilation (NIV) such as nasal continuous positive airway pressure (CPAP) in preterm infants is a reasonable alternative to early intubation after birth. Recently, nasal CPAP has shown a benefit with a small reduction in the risk of the combined outcome of death or bronchopulmonary dysplasia. There has been an upsurge in the use of NIV as primary therapy for preterm infants, bringing with it the dilemma of when and how to give exogenous surfactant. In an effort to overcome this problem, minimally invasive surfactant therapy (MIST) to spontaneously breathing infants, allows them to remain on CPAP in first days after birth. MIST has included administration of exogenous surfactant by brief tracheal catheterization, aerosolization, laryngeal mask, and intrapharyngeal instillation. In recent clinical trials, surfactant delivery via brief tracheal catheterization was found to reduce the need for subsequent intubation and mechanical ventilation and to improve short-term respiratory outcomes. In conclusion, MIST is gentle, safe, feasible and effective to perform in preterm infants and will also be used commonly in Korea.

재태연령 32주 미숙아에서 재태연령군에 따른 낭종성 뇌실 주위 백질 연화증 발생 위험 요인

심규홍 ( Gyu Hong Shim ),최명재 ( Myoung Jae Chey ) 대한주산의학회 2016 Perinatology Vol.27 No.1

목적: 본 연구는 32주 미만의 미숙아에서 재태연령군에 따른 낭종성 뇌실주위 백질연화증의 발생률과 발생에 대한 위험 요인을 알아보고자 시행하게 되었다. 방법: 대상은 2009년 1월부터 2015년 6월까지 인제대학교 상계백병원에서 출생하고, 신생아 중환자실에 입원하여 4주이상 생존한 재태연령 32주 미만의 미숙아를 대상으로 후향적 의무기록 분석을 통하여 연구를 진행하였다. 이를 토대로 낭종성 뇌실주위 백질연화증의 발생률과 발생에 대한 위험 요인에 대한 조사를 재태연령군에 따라 진행하였다. 결과: 32주 미만의 미숙아에서 낭종성 뇌실주위 백질연화증의 발생률은 15.1% (26/172) 였다. 전체 환자군에 대한 다변량 분석에서는 이중 grade 2 이상의 뇌실 내 출혈[P=0.006, odds ratio (OR) 5.478, 95% confidence interval (CI)1.641-18.285], 28일 이상의 산소 사용 유무(P=0.025, OR 3.086, 95% CI 1.152-8.264) 및 stage 2 이상의 신생아 괴사성장염(P=0.042, OR 3.731, 95% CI 1.047-13.333)이 독립적인 위험 요인으로 분석되었다. 28주를 기점으로 세분화 하였을 대 28주 미만에서는 임신성 고혈압과 뇌실 내 출혈이 독립적인 위험 요인이었고, 28-31주의 미숙아에서는 28일 이상의 산소 사용 유무 와 배양 검사로 확진된 패혈증 유무가 독립적인 위험요인으로 나왔다. 결론: 이러한 결과는 임신성 고혈압과 뇌실 내 출혈은 28주 미만의 미숙아에서 낭종성 뇌실주위 백질연화증의 발생 위험을 증가시킬 수 있다는 것을 보여주고, 28일 이상의 산소 사용과 배양 검사로 확진된 패혈증은 28+0-31+6주 미숙아에서 낭종성 뇌실주위 백질연화증의 발생 위험을 증가시킬 수 있다는 것을 보여준다. Purpose: The aim of the study was to determine the incidence of cystic periventricular leukomalacia (PVL) and identify the risk factors for cystic PVL in preterm infants with gestational age (GA) less than 32 weeks according to gestational age group. Methods: The medical records and brain imaging were reviewed for preterm infants with less than 32 weeks GA who lived more than 4 weeks and admitted to the neonatal intensive care unit at Inje University Sanggye Paik Hospital from January 2009 to June 2015. We determined the incidence and the risk factors for the development of cystic PVL in preterm infants according to GA group. Results: Incidence of cystic PVL was 15.1% (26/172). Multivariate analysis showed that intraventricular hemorrhage (IVH) [P=0.006, odds ratio (OR) 5.478, 95% confidence interval (CI) 1.641-18.285), oxygen uses over 28 days (P=0.025, OR 3.086, 95% CI 1.152-8.264), and NEC (P=0.042, OR 3.731, 95% CI 1.047-13.333) were independent risk factors for the developmental of cystic PVL. Subgroup analysis showed that pregnancyinduce hypertension and IVH were independent risk factors in preterm infants with GA of less than 28 weeks. Also, oxygen uses over 28 days and culture proven sepsis were independent risk factors in preterm infants with GA of 28+0-31+6 weeks. Conclusion: These results suggest that pregnancy-induce hypertension and IVH may increase the risk for the subsequent development of cystic PVL in preterm infants with GA of less than 28 weeks, and oxygen uses over 28 days and culture proven sepsis may increase in preterm infants with GA of 28+0-31+6 weeks.

극소 저출생 체중아에서 신석회화증의 위험인자

심규홍,이진아,신윤정,김이경,박준동,김병일,최중환 대한소아과학회 2004 小兒科 Vol.47 No.3

Antimicrobial Activities of (-)Epicatechin from Ulmus davidiana var. japonica Cortex

Gyu-Hee Lee,Chang-Ju Shim,Yeong-Il Chang,Seong-Hyun Choi,Hong-Rock Oh,Man-Jin Oh 한국식품영양과학회 2001 Preventive Nutrition and Food Science Vol.6 No.4

The extract of Ulmus davidiana var. japonica cortex has known as natural anti-inflammatory substance in East Asia. For the identification of antimicrobial substance, it was extracted by using methanol, fractionated by using different organic solvents. The fraction of butanol was represented the highest antimicrobial activities. Therefore, the butanol fraction was purified, identified the chemical structure by ¹H, ¹³C-NMR spectra, FT-IR, EI/MS spectroscopies. The isolated antimicrobial substance was identified as cis-2-[3,4-dihydroxy phenyl]-3,4-dihydro-2H-1- benzopyran-3,5,7-triol, which has commonly known as (-)epicatechin. Its minimum inhibitory concentrations (MICs) against Staphylococcus aureus, Listeria monocytogenes were shown as 100 μg/mL, 500 μg/mL, respectively.

부분 간절제술로 급성 간부전이 유도된 흰쥐에서 동종 동결간세포의 비장내 이식의 효과

홍성표,조주영,이준성,김경철,이문성,황성규,김연수,윤익진,박필원,임규성,심찬섭 대한간학회 1999 Clinical and Molecular Hepatology(대한간학회지) Vol.5 No.2

Background/Aims : Various techniques of hepatocyte transplantation were actively studied as an alternative to liver transplantation, because of the difficulty of obtaining donor organ, technical difficulties, and high cost. Isolated hepatocytes could be appropriately banked and distributed on demand. We tried to investigate the effect of intrasplenic transplantation of allogenic cryopreserved hepatocytes, into spleen prior to 90% partial hepatectomy in rats, on the survival rate. Methods : Cryopreserved hepatocytes, isolated by collagenase perfusion of the liver via the portal vein, were thawed and transplanted into the spleen of rats prior to induction of acute hepatic failure by resection of all lobes except caudate lobe (2.0×107 hepatocytes/rat). Results : 1. The viability of freshly isolated hepatocyte was 70-85%, but cell viability after cryopreservation 30-50%. 2. Difference of survival in control and transplant group is not statistically significant. but the survival rate, 48 hours after 90% partial hpatectomy, for control (7) and transplanted group (11) were 0% and 18%, respectively. 3. Although the glucose reduction gradient was not significantly different between two groups, it was more prominent in the control group than in the transplanted group. 4. Engraftment and survival of transplanted hepatocytes were noted in the spleen 2 days after transplantation. Conclusions : We could not observe statistically significant improvement of survival with intrasplenic transplantation of cryopreserved hepatocytes in rats with 90% partial hepatectomy-induced acute liver failure. However, 18% survival after 90% partial hepatectomy was noted in the transplanted group, compared to no survival in the control group. This suggests that intrasplenic transplantation of cryopreserved hepatocytes might be effective in the treatment of acute liver failure. (Korean J Hepatol 1999;5:116-123)