Lactobacillus plantarumCLP-1이 돼지바이러스에 미치는 효과

이건희(Gun-Hee Lee),김영희(Young-Hee Kim),조현아(Hyeon-A Cho),강성기(Sung-Gi Kang),김동건(Dong-Gun Kim) 한국생물공학회 2011 KSBB Journal Vol.26 No.1

To isolate Lactic acid bacteria for animals, we have screened from Kim-chi, swine intestine, swine feces, and dairy products by random selection and anti-viral, antipathogenic bacteria test. Among them, CLP-1 shown that inhibitory effect against rotavirus, porcine epidemic diarrhea (PED) virus, Salmonella sp, and E.coli. By examining biological property, API-ZYM and identified Lactobacillus plantarum by 16S rDNAgene sequence. CLP-1 determined resistance to low pH and bile salt. Futhermore, the cell body of CLP-1 adhered to the intestinal epithelium tissue of swine and Caco-2 cell. CLP-1 was examined on cell immune system modulating activity in vitro. The whole cell and cell culture supernatant was increasing of interferon-β activity. And then, CLP-1 increased prevention effect by Salmonella enteritidis infection in SPF chickens. And we determined similar result in pigs.

조혈모세포이식 환자에서 침습성 진균 감염에 대한 Micafungin의 예방 효과 및 안전성

김시현,이동건,최수미,권재철,박선희,최정현,유진홍,이성은,조병식,김유진,이석,김희제,민창기,조석구,김동욱,이종욱,민우성,박종원 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Background: Micafungin, a potent inhibitor of 1,3-β-D-glucan synthase, is a novel antifungal agent of the echinocandin class. In vitro study showed that micafungin was effective against Aspergillus species as well as Candida species, but clinical data on the prophylactic efficacy against invasive fungal infections (IFIs) other than candidiasis are still lacking. Materials and Methods: We identified 60 consecutive adult hematopoietic stem cell transplantation (HSCT) recipients who received at least 3 doses of micafungin during neutropenic period. Micafungin was started as an alternative in patients who were intolerant or had adverse events (AEs) to primary prophylactic antifungal agents. We retrospectively reviewed the medical records and analyzed the efficacy and safety of micafungin for prophylaxis against IFIs. Results: The patients either had autologous (n=9) or allogeneic (n=51: 1 syngeneic, 24 sibling, 26 unrelated donor) HSCT. Itraconazole oral solution (n=58) was the most frequently used first line antifungal agent for prophylaxis and was administered for median 11 days. The most frequent cause of switch to micafungin was vomiting (n=42). The duration of neutropenia and micafungin administration was median 13 and 12 days, respectively. A successful outcome was achieved in 45 (75%) patients. Empirical antifungal therapy was initiated in 13 (22%) patients. There were 2 cases (3.3%) of breakthrough fungal infections which comprised a probable invasive pulmonary aspergillosis and a possible invasive fungal sinusitis. There was no case of invasive candidiasis. A total of 53 (88%) patients experienced at least one AE regardless of causality during micafungin administration. The most frequent AEs were hypokalemia, vomiting, diarrhea, and elevated serum aspartate aminotransferase or alanine aminotransferase. Among the aforementioned AEs, only 1 case of diarrhea could be classified as a probable relation with micafungin when causality was assessed. There was no AEs that caused discontinuation of micafungin. Conclusions: Micafungin seems to be a safe and effective agent for prophylaxis of IFIs including aspergillosis as well as candidiasis in HSCT recipients. However, further large, prospective, and randomized comparative studies are warranted for aspergillosis.

입원 중인 베트남 참전 군인의 삶의 만족도와 관련된 요인

김애리,박종,김율,노희송,박광희,김은숙,김형철,홍강식,류소연,강명근,김기순 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Background and Objectives: The aim of the study was to identify the factors associated with life satisfaction of hospitalized Vietnam veterans. Material and Methods: The data were collected from 200 hospitalized Vietnam veterans of 5 veterans hospitals in Korea from September 13 to 23, 2004. Information of general characteristics, health behavior, disease history, family function, depression and life satisfaction were collected by structured questionnaire. Results: The mean of life satisfaction was 21.48(±6.03), and the mean of depression was 53.10(±9.64). The prevalence of depression was mild depression 24.0%, moderate depression 43.5% and severe depression 25.0%. Depression was associated with life satisfaction and depression was higher, life satisfaction was lower. Family function and age were positively associated with life satisfaction, Conclusion: Life satisfaction was affected by depression and family function in hospitalized Vietnam veterans. Therefore, it was thought that psychosocial approaches such as improve depression and family function in care of hospitalized Vietnam veterans is required.

플라즈마 이온주입기에서의 시변환 쉬스 연구

김곤호,김건우,김영우,최영도,김종식,김상진,한승희,홍문표 漢陽大學校 工學技術硏究所 1998 工學技術論文集 Vol.7 No.1

Abstract- In a plasma source ion implantation, the target is successively biased by negative voltage pulses with an intrinsic finite rise time, resulting in the time-dependent sheath formation around the target. Measurement of time-dependent sheath during the pulse rise show the ion matrix sheath development which is in proportion to square root of time and pulse rise rate over plasma density but independent of the ion mass.

조혈모세포이식 환자에서 침습성 진균 감염에 대한 이트라코나졸 액과 플루코나졸 시럽의 예방 효과 : 전향적, 무작위, 비교 임상시험

최수미,이동건,최정현,박선희,엄기성,김유진,김희제,민창기,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2005 감염과 화학요법 Vol.37 No.2

목적 : 조혈모세포이식 환자에서 호중구 감소 기간 동안 이트라코나졸 액(ITZS)과 플루코나졸 시럽(FCZS)의 진균 감염에 대한 예방 효과를 전향적으로 비교해 보고자 하였다. 재료 및 방법 : 2001년 8월부터 2002년 6월까지 가톨릭 조혈모세포이식센터에 입원하여 동종 조혈모세포이식을 받은 만 18세 이상의 환자들을 각각 ITZS와 FCZS군으로 무작위 배정하여 침습성 진균 감염 발생과 약제 관련 부작용을 전향적으로 조사하였다. 결과 : ITZS군에 40명, FCZS군에 38명이 배정되었고, FCZS군 환자의 평균 나이가 더 많았다. 시험 약제 평균투여기간은 ITZS군 16.4일, FCZS군 21.9일로 ITZS군이 더 짧았다. 이는 ITZS군에서 소화기 부작용으로 인한 약제 중단이 더 많았기 때문으로 보인다. 시험 약제 투여 후 총 빌리루빈 수치의 유의한 증가가 관찰되었으나, 약제 관련 부작용은 모든 환자에서 가역적이었다. 호중구감소 기간동안 표재성 진균 감염은 두 군에서 모두 발생하지 않았고, 침습성 진균 감염은 ITZS군 5명(12.5%), FCZS군 8명(21.1%)으로 FCZS군에서 많았으나, 통계학적으로 유의하지 않았고, 사망률에도 유의한 차이는 없었다. 결론 : 침습성 진균 감염에 대한 이트라코나졸 액과플루코라졸 시럽의 예방 효과에 유의한 차이는 없었으나, 앞으로 더 많은 환자를 대상으로 잘 짝지어진 대조군 연구가 필요할 것으로 보인다. 부작용 중 간독성과 관련하여 이식시 투여되는 여러 약제들과의 상호작용에 대한 연구도 필요할 것이다. Background : Though fluconazole is widely used for antifungal prophylaxis, it is ineffective against mould infections including Aspergillus species. Itraconazole has a broader spectrum than fluconazole but the capsule form shows erratic bioavailability in neutropenic patients. In this study, we compared itraconazole oral solution (ITZS) with fluconazole syrup (FCZS) for the prevention of invasive fungal infection (IFI) in allogeneic hematopoietic stem cell transplant recipients. Materials and Methods : Adults receiving allogeneic hematopoietic stem cell transplantation (HSCT) from september 2001 to June 2002, were randomly allocated to either the ITZS group or the FCZS group. We prospectively evaluated the safety and efficacy of each drug. Results : Out of 78 patients (40 patients in the ITZS group and 38 patients in the FCZS group) who were eligible for this study, 37 patients completed the course of prophylaxis without any evidence of IFI. The mean duration of prophylaxis was 16.4 days for the ITZS group and 21.9 days for the FCZS group (P<0.006). Drug-related adverse events occurred in 28 patients (70.0%) and 19 patients (50.0%) in the ITZS group and the FCZS group, respectively. Common adverse events of ITZS were nausea, vomiting, and diarrhea. Drug-related reversible hepatotoxicity occurred in 4 patients in the ITZS group. There was a significant elevation of total bilirubin level in the ITZS group. The incidence of suspected IFI occurred in 5 patients (12.5%) who received ITZS, compared with 8 (21.1%) who received FCZS (P=0.372). There were no proven IFIs or superficial (oral/vaginal) fungal infections in both groups. Overall mortality was not different between the two groups (2.5% in the ITZS group versus 5.3% in the FCZS group, P=0.610). Conclusion : ITZS and FCZS showed similar protection against IFI during pre-engraftment period. Poor tolerability due to gastrointestinal troubles of ITZS might limit its success as prophylactic therapy. Well matched controlled study with large number of patients will be required in the future.

조혈모세포이식 환자에서 발생한 Cytomegalovirus 질환의 특징 : 일개 대학변원에서 최근 10년간의 경험

최수미,이동건,박선희,김시현,김유진,민창기,김희제,이석,최정현,유진홍,김동욱,이종욱,민우성,신완식,김춘추 대한감염학회 2009 감염과 화학요법 Vol.41 No.1

Background : Studies on cytomegalovirus (CMV) diseases in Korean hematopoietic stem cell transplant (HSCT) recipients are lacking and do not reflect the recent trends of advances and changes. Therefore, we tried to analyze the clinical features of CMV diseases in HSCT recipients over the past 10 years at a tertiary university hospital in Korea. Methods : Retrospective review of medical records was done for all adult HSCT patients who received transplant at the Catholic HSCT Center from January 1998 to January 2008. Results : Forty-four cases (2.2%) of CMV diseases were identified. CMV pneumonia was diagnosed in 17 patients, retinitis in 16 patients, enterocolitis in 7 patients, esophagitis 1 patient, gastritis in 1 patient, duodenitis in 1 patient, and hepatitis in 1 patient. The median onset of symptom was 90 days after transplantation. Late CMV diseases accounted for 47.7%. CMV related death varied from 0 to 58.8% according to the involved organ. CMV retinitis was diagnosed relatively later in the course of transplantation mostly in patients who had chronic graft versus host disease (GVHD). On the contrary, CMV enterocolitis mainly occurred in patients who suffered from acute GVHD. The overall concurrent CMV reactivation was documented to be 63.6%: the concurrent CMV reactivation was observed only in 37.5% of patients with retinitis. Conclusions : We observed some differences in the pattern of CMV disease manifestation according to the involved organ and reconfirmed the fact that CMV pneumonia is the most common and fatal disease in HSCT recipients. Additionally, CMV retinitis was not uncommon in HSCT recipients. Since specific marker does not exist in predicting retinitis, regular ocular examination should be done thoroughly, especially in patients with chronic GVHD.

화상처리를 이용한 지능형 가공시스쳄의 설계 및 구현

김관형,김수정,김영탁,문희근,이상배 한국해양대학교 산업기술연구소 2003 연구논문집 Vol.20 No.-

본 논문은 자동화 시스템에 적용 가능한 지능 제어 시스템에 관한 연구이다. 따라서 본 연구에서는 지능제어 이론과 영상처리기술들을 이용하여 지능형 복합제어시스템을 능동적으로 제어하는 것을 목적으로 한다. 지능형 복합제어시스템의 성능 평가와 제어 전략은 각각 신경회로망과 퍼지이론을 이용하였고, 영상 처리기법을 이용하여 물체의 특정 위치를 추출하였다. 실제 시스템에 적용하여 얻은 실험 결과를 통하여 본 논문에서 제안한 방버의 유효성을 제시하고자 한다. This paper is research into intelligent control system that is able to apply at automatic system. So, in this paper we are in control of intelligent compound control system actively for the use of intelligent control theory and image processing. Performance evaluation and control strategy of intelligent compound contro system were used to neural network and fuzzy theory, we proposed detection of particular position using image processing we propose availability of proposed method based experiment result that apply to real system in this paper.

2019년도 2학기 신라대학교 재학생의 부산인식 설문조사 분석 : 인문사회계 학생을 대상으로

김대래, 신군재, 유영명, 장희정, 정쾌영 신라대학교 부산학연구센터 2016 부산연구 Vol.17 No.2

이 논문은 신라대학교에 재학 중인 학생들을 대상으로 부산에 대한 인식을 알아보기 위해 수행 한 설문지에 대한 분정확히 알아보기 위하여 분석에서는 부산출신자를 대상으로 하였다. 학생들과 부모의 출생연도와 연령 등에 관한 기초분석을 하고 부산의 자랑과 특성에 관한 인식을 분석 하였다. 아울러 학생들의 일석이다. 총 237부의 응답지를 받았는데 그 중에서 부산출생자 153명 을 대상으로 분석을 하였다. 부산에 관한 인식을 좀 더 상생활과 관련된 설문을 분석하였다. 분석에는 spss24를 이용하여 빈도분석과 기술통계분석 그리고 교차분석을 하였다.

회전수에 따른 발레 삐루엣 앙드올 동작의 분석

김은희,이건범,이기청 한국운동역학회 2001 한국운동역학회지 Vol.11 No.3

The purpose of this study was to investigate the difference between the performance and the increase of the number of turn in Ballet Pirouette en de hors. Single turn, double turn, triple turn, and maximum turn of four Ballerinas, who have more than ten-year experience in Korea Ballet Company, was filmed by two 16mm high speed cameras, and used AMTI force platform. DLT method was employed to obtain 3-D coordinates. The results were as follows: 1. The COP displacement was more larger as increases of the number of turn. 2. It is importance to maintain the COG highly as possible, at initial step for the effective multiple turn. 3. In order to perform multiple turn effectively, it would be desirable to maintain consistent position regardless of the number of turn and to which the angle of elbow at the touchdown phase. 4. If the time required of turn were reduced at the starting point and the speed were kept to final interval, the movement of multiple turn could be performed smoothly.

배추 종자 및 어린 싹의 성장 시기에 따른 3-butenyl isothiocyanate 및 total glucosinolates 함량 변화

김연경,김건희 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

배추의 품종별 종자 및 성장 시기에 따른 3-butenyl isothiocyanate 및 total glucosinolates를 분석하기 위하여 동풍 배추(Winter Pride) 및 55일 배추(55 Days) 품종의 종자, 발아 1일, 3일, 5일, 7일 된 어린 배추를 떡잎(cotyledon), 배축(hypocotyl), 뿌리(root) 부분으로 나누고 anion-exchange column(Dowex 1×2-100)을 통과시키고 아세톤 침전법을 사용하여 제조한 crude myrosinaes로 반응시켜 함량 변화를 조사하였다. 종자에서는 55일 배추의 3-butenyl isothiocyanate의 함량이 동풍 배추 종자보다 다소 높았다. 55일 배추의 3-butenyl isothiocyanate 함량은 3-day-old seedlings의 떡잎과 배축에서 가장 높았으며 동풍 배추의 떡잎에서는 3-day-old seedlings까지 함량이 증가한 후 감소하였으며 배축은 3-day-old seedlings까지 함량이 증가하였다. Total glucosinolates를 분석한 결과 종자에서는 동풍 배추보다 55일 배추의 함량이 다소 높게 조사되었으며 55일 배추의 떡잎은 3-day-old seedlings에서 가장 높은 함량을 보였다. 배축 부분은 7-day-old seedlings가지 증가하였으며 뿌리 부분의 glucosinolates는 성장 기간동안 점차 감소하는 경향을 나타내었다. 동풍 배추의 떡잎과 배축 부분은 5-day-old seedlings까지 증가하다가 감소하였다. The main objective of this study was to investigate the pattern of glucosinolates, in terms of 3-buteny1 isothiocyanate, and total glucosinolates, in different parts of young seedlings and seeds in Korean Chinese cabbages. For determination of glucosinolates, 55 days and Winter pride cultivars of Korean Chinese cabbages seed and different parts of 1, 3, 5, and 7-day-old seedling were used for analytical sample preparation provided with an anion exchanges column, and measured by GC and UV-visible Spectrophotometer. The concentration of 3-buteny1 ITC was higher in the cotyledon and the hypocoty1 parts of 55 days cultivar seedling than those of Winter Pride cultivar. In the cotyledon parts of Winter pride cultivar contained the highest concentration of total glucosinolates while those were increased in the hypocotyl and decreased in the hypocotyl parts of seedling in Winter pride cultivar. There was no significant difference of total glucosinolates in the root part. In the seeds, 55 days cultivar concentraion was higher than Winter pride cultivar both of 3-butenyl ITC and total glucosinolates. In our study, the levels of 3-buteny1 ITC and total glucosinolates depended on cultivars, growth stages, and parts in Korean Chinese cabbage.