성장호르몬 분비성 뇌하수체 선종에서 소마토스타틴 수용체 (제2아형, 제5아형), G_i2α 및 Pit-1 유전자 발현

류미숙,양인명,박철영,우정택,김성운,김진우,김영설,최영길,김은희,박승준,김국기 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.2

Background: Mutation of Gs protein subunit (gsp oncogene), detected in about 30∼40% of growth hormone (GH)-secreting pituitary tumors, is associated with an increased long-acting somatostatin analog octreotide sensitivity. However, the mRNA expression of somatostatin receptor (sst) was not changed in the GH-secreting pituitary tumor, regardless of whether they were gsp oncogene positive or negative. This suggests that the expression of genes coding for G_i2α, Pit-1 and the other factors involved in the regulation of secretory activity in somatotrophs is likely to be altered in gsp oncogene positive tumors. We observed the impact of the gsp oncogene on the expression of the genes coding for Gi2, Pit-1 and sst (2&5) in GH-secreting pituitary tumors. Methods: The GH response to octreotide was examined in 13 acromegalic patients before transsphenoidal adenomectomy. Genomic DNA and RNA were extracted from fresh frozen tumor tissues. PCR was performed to amplify and sequence the region between codon 184 and 251 that includes exons 8 and 9 of the Gs gene. Sst2, sst5, G_i2α and Pit-1 mRNA levels were measured by semi-quantitative RT-PCR. Results: Sst2 and sst5 mRNA transcripts were detected in all tumors (7 gsp +, 6 gsp-). The amount of sst transcripts varied considerably varied between the tumors. There were no significant differences in sex, age, tumor size, grade or basal GH levels. Pit-1 and sst2 mRNA levels were not different. In contrast, G_i2 mRNA levels were significantly higher in gsp (+) while sst5 mRNA levels were higher in gsp (-). Conclusion: These data suggests that gsp oncogene may increase Gi2α levels but decrease sst5 mRNA levels. However, Pit-1 and sst2 mRNA expression may not be affected by gsp oncogene. The increased expression of the G_i2α gene might be an inhibitory compensatory response to the action of gsp oncogene

성인 막성신병증의 임상적 고찰

양종오,이상주,박기현,장윤경,이강욱,서광선,신영태 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Membranous nephropathy (MN) is a glomerular disease characterized by diffuse thickening of the glomerular basement membrane without significant mesangial proliferative change and is usually manifested by the nephrotic syndrome. To evaluate the clinical and pathologic characteristics of this disease in adults, we analyzed 65 patients with primary membranous nephropathy who were diagnosed at Department of Internal Medicine, Chungnam National University Hospital from February 1986 to February 2001. The results are as follows: 1) Of total 96 patients with membranous nephropathy, 67.7% was primary MN. Hepatitis B-associated MN and lupus MN were 22.9%, and 9.4%, respectively. Median duration of follow-up was 35.1 months(0.3∼177) in primary MN, 49.6 months(2.5∼103) in lupus MN, and 35.6 months(0.5∼108) in hepatitis B-associated MN. 2) Mean age of the patients with primary MN at the time of diagnosis(43.3 years) was significantly higher than that of lupus MN(30.4 years) and HBV-associated MN(36.2 years). Male to female ratio of primary MN was 1.03:l. 3) At the time of diagnosis, 89.2% of primary MN was presented with nephrotic syndrome. Twenty seven percent of patients showed hypertension. Hematuria was found in 58.5% of primary MN patients. Azotemia was noted in 3.1 %. 4) Global sclerosis, tubular atrophy and interstitial fibrosis were noted in 39.8, 16.1 and 11.3% of primaty MN, respectively. IgG and C3 were deposited on the glomerular capillary loop in 92.7% and 29% of primary MN patients. There was no significant correlation between the electron microscopic pathologic stage and clinical findings. 5) Of 56 patients with primary MN followed more than 6 months, 50(90.9%) patients were treated with prednisolone, cyclophosphamide or cyclosporin. Complete remission was obtained in 29 patients(51.8%), partial remission in 6 patients(10.7%), and no response in 18 patients(32.1%). Of 6 patients who were not treated with immunosuppressive drugs, 3 patients showed spontaneous complete or partial remission. Three patients who did not show clinical improvement progressed to end-stage renal failure. There were no significant clinical difference including renal pathology at the time of diagnosis between the patients who showed complete or partial remission and patients who did not. In conclusion, it was difficult to predict the long-term prognosis of primary MN at the time of diagnosis, clinically. The response to therapeutic modality would be the most important to predict long-term prognosis of primary MN.

소실문자 판독에 관한 연구

양후열,김동욱,최섭,진명수,이영수,나기현 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

L - NAME 주입시 신 교감신경이 신혈류량 변화에 미치는 영향

민영기,양훈모,이석호,김종규 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

Recently it is known renal medullar will be play a important role of blood pressure control and pressure natriuresis. Renomedullary antihypertensive mechanism is activated by increasing perfusion pressure of kidney and can be inhibited by sympathetic nerve stimulation or blockade of nitric oxide(NO) formation. It is important to know what is affect on renal regional blood flow, especially renal medullary blood flow(MBF). For the purpose of investigating the effect of the renal sympathetic nerve on regional blood flow during infusion of blocking NO synthesis(by N^(ω)-nitro-L-arginine methylester:L-NAME) in spontaneous hypertensive rat(SHR) and Wistar rats normal control, Mean arterial pressure(MAP), total renal blood flow(TRBF), midcortical blood flow(CBF), inner medullary blood flow were measured in innervated or denervated renal sympathetic nerve group of SHR and Wister rats. All group that received the L-NAME were seen increasing MAP while TRBF, CBF and MBF decreased and the decrease of TRBF in SHR is more marked than in Wistar rat. In spite of increasing the MAP, MBF in all four group decreased, respectively C: 220 ± 3 U, 205 ± 2 U Wi : 215 ± 4 U, 165 ± 12 U Wd :218 ± 3 U, 170 ± 10 U Si :195 ± 4 U, 98 ± 15 U Sd : 200 ± 3 U, 120 ± 14 U, especially the most remarkable decrease about 50 % in SHR innervated group. Thus, according to these result, NO seems to maintain the MBF and the partial interaction between renal sympathetic nerve and NO in medullary blood flow can be suggested in innervated SHR.

본태성 혈소판 증다증 2예

주기산,임현성,김미자,양태영,허경,김완중,박유환,정춘해 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Essential thrombocythemia is a chronic myeloproliferative disorder of unknown origin, charcterized by excessive number of morphologically and functionally abnormal platelet in peripheral blood and abnormal proliferation of megakaryocytes in bone marrow. Its main clinical manifestations are hemorrhages and thromboses. The aim of therapy is suppression of the excessive bone marrow activity, which can be achieved by radioactive phosphorus, alkylating agent, hydroxyurea and interferon. Two cases were diagnosed at our hospital which had laboratory and clinical features compatible with this disease. We have successfully treated patient with hydroxyurea and platelet antiaggregating agent.

제주도 동북부지역 지하수의 수위변동과 수질조성에 관한 연구

고기원,양성기,문영석 제주대학교 환경연구소 1995 환경연구논문집 : 제주대 Vol.3 No.1

The groundwater levels at 5 sites, the ve.rtid variations of the electric cmductivity at 7 sites and the water quality of the groundwater at 11 sites were measured to study the charactmistics of the groundwater levels' fluctuations and occurrence of eastnorthem part Cheju Island. The result of examination of subsurface geologic structure of the Kujwa area from core logging shows that the area does not have the Seoguipo Formation that acts an aquiclude in the Shinchon area by preventing the downward flow of the hroundwater, In addition, the result also shows that the thickness of basaltic flows below the mean sea level in the Kujwa area is much larger than that the Shinchon area, and this suggests that the Kujwa area moved downward bu sunsidence. The groundwater levels at Kimyong and Kujwa areas occurred in response to oceanic tides. The tidal effect on the groundwater levels upon the distance from seashore. However, structure of the Kujwa area The result of examination of suMace lnnuenced by the amount of precipitation The groundwater qualities of the Kujqa area characterized by Na-Cl type, and that of the Shinchon area by Na-HCO₃type, The electric conductivity peofiles of the groundwater, the Kujwa area is increase with water depth, but Shinchon area is on the decrease or uniformly with water depth, Thes dofference indicates that the groundwater of both areas has different origin and different geologic environments.

제주도 연안해역에 출현하고 있는 적조생물

윤양호,노홍길,김영기 제주대학교 해양연구소 1991 해양자원연구소연구보고 Vol.15 No.-

대황황련해독탕의 항고지혈증 작용

김영석,정은아,장종철,양형길,김남재,조기호,배형섭,이경섭,김동현 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2001 No.-

Whangryunhaedok-Tang (WT) is formulated with Coptidis Rhizoma, Phellodendri Cortex, Scutellariae Radix and Gardeniae Fructus, and Daewhang-whangryunhaedok-Tang (DWT) is made by the combination of Rhei Rhizoma, a wellknown anticostipation drug in WT. Therefore, DWT has been evaluated for antihyperlipidemic effects on experimental hyperlipidemic rats and mice induced by corn oil and high cholesterol-diet. Oral administration of DWT significantly inhibited the increase of serum triglyceride and LDL-cholesterol levels, and the decrease of serum HDL-cholesterol levels in hyperlipidemic rats induced by corn oil. Also, oral administration of DWT significantly prevented the increase of serum total cholesterol, triglyceride and LDL-cholesterol, and liver total cholesterol and triglyceride in 1% cholesterol-diet fed mice. These results suggest that DWT is effective for the treatment of hyperlipidemia.

제주북방, 함덕 연안해역에 있어서 식물플랑크톤 군집의 계절변동 특성

윤양호,노홍길,김영기 제주대학교 해양연구소 1992 해양자원연구소연구보고 Vol.16 No.-

Field survey for oceanographic conditions and the seasonal succession of phytoplanktan were carried out in the Hamdok port from April 1989 to February 1990. Water temperature ranged between 11.4℃ in January and 27.3℃ in August, and salinity fluctuated greatly, with a maximum of 35.6960 in August and minimum of 21.8% in April. A total of 120 species of phytoplankton belonging to 69 genera were observed in which, at least, 8 genera, Oxyphysis, Amphidinium, Cochlodinium, Torodinium, Nematodinium, Katodinium, Ebria and streptoheca are first records in the Cheju coastal waters. The predominant species are centric diatoms. Skeletonema costatum, Chaetoceros spp. through the year, while they are unarmoured phytoflagellates, Heterasigma akashiwo, Gymnodiniurn sp., Gyrodinium sp. in warmer seasons. And phytoplankton cell number fluctuated between 4.1~10^(3) cells/l in winter and 2.4~10^(4) cells/l in spring blooms, and it was under the control of centric diatoms for a year. On the other hand, it is occupied by phytoflagellates including some unarmoured dinoflagellates in summer season.

대황황련해독탕의 사염화탄소 유발 간장해 보호효과 미치 급성독성

김영석,정은아,장종철,양형길,김남재,조기호,배형섭,이경섭,김동현 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2002 東西醫學硏究所 論文集 Vol.2002 No.-

ABSTRACT - This study was performed to evaluate hepatoprotective effect of daewhang-whangryunhaedok-Tang(DWT) on liver injured rats induced by CCI_4 and the acute oral toxicity of it in mice. The activities of serum transaminase(ALT/AST), alkaline phosphatase(ALP) and lactic dehydrogenase(LDH), the levels of serum total cholesterol(TC) and triglyceride(TG), change of liver enlargement, and inhibitory activities of lipid perotidation, catalase and glutathione-S-transfrease(GST) in liver microsome were determined in hepatotoxic rats induced by CCI_4. DWT was significantly reduced the serum ALT, AST, ALP, LDH. TC and TG lecels. And, the increase of lipid peroxidation, decrease of catalase and GST activities in the liver microsome of CCI_4-intoxicated rat were significantly improved by the treatment of DWT. Male and female mice were administered maximum dosages of 5.000 mg/kg b.w. of DWT. After single oral administration of DWT to mice, we observed them daily for 2 weeks.DWT did not induce any toxic signs in the mortalitie, clinical signs, body weight changes, and gross necropsy finfings of mice. Based in these results. It is concluded that DWT may have the hepatoprotective effect on CCI_4 induced hepatotoxicity in rats. Also. DWT may have no side effect and its LD_50 value may be over 5.000mg/kg b.w. in mice.